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931.
932.
Single rays as indicators of fin vestigialization in coho salmon: patterns and perspectives 总被引:1,自引:0,他引:1
W. BRUCE CAMPBELL 《Biological journal of the Linnean Society. Linnean Society of London》2000,71(4):789-799
Although finrays in salmon normally contain a pair of elements (biramous), finrays with a single element (uniramous) occasionally develop. Exposure to chronic stress during character development has been shown to increase fluctuating asymmetry, suggesting the occurrence of single finrays may be stress‐induced. On the other hand, single finrays may be evolutionary atavisms, reflecting fin vestigialization caused by reduced selection pressure. To assess the merits of these hypotheses, cleared and stained paired and median fins were examined for single finrays in juvenile coho salmon (Oncorhynchus kisutch Walbaum) from two compatible hatchery stocks and their reciprocal hybrids which had been exposed to different patterns of chronic temperature fluctuation throughout embryogenesis. In the median fins, uniramous secondary finrays predominated, and single primary finrays were infrequent. Single finrays in the median fins did not respond to thermal treatment or cross, suggesting the fins were evolutionarily stable and under strong developmental control. The paired fins were observed to contain only primary finrays. Frequencies of single pelvic finrays increased under thermal stress, as did fluctuating asymmetry, suggesting increased sensitivity to stress due to reduced developmental control in this fin. However, the presence or absence of single finrays in the paired fins did not alter the statistical significance of the conclusions regarding levels of fluctuating asymmetry, the number of asymmetric fish, or the contribution to meristic variation from asymmetry. Locations of single finrays in the paired fins were unaffected by thermal treatment or cross. Single finrays were most commonly observed in the trailing margins of both paired fins, a finding consistent with vestigialization theory. Frequency histograms of single pectoral finray locations revealed a second peak in the leading quarter of the fin. The esults support the hypothesis that single primary finrays are evolutionary atavisms, and that reduced selection pressure is differentially influencing the paired fins. 相似文献
933.
大鼠脊髓后角内神经降压素对一级传入C纤维突触前抑制效应的形态学证据(英文) 总被引:2,自引:0,他引:2
本研究的目的是为了揭示大鼠脊髓后角内的神经降压素是否对一级传入C纤维进行突触前调节,荧光显微镜下观察到,在脊髓Ⅰ-Ⅲ层内,和压素样免疫反应性(NTLI)的分布与来源于西非单豆素的同工凝集素Ⅰ-B4(Ⅰ-B)的结合位点到分布有部分重叠,在其聚集激光扫描显微镜下进一步观察显微镜下,在蛛网膜下腔注射辣椒素的大鼠脊髓后角浅层内,一些NTLI阳性终末与变性终末形成突触性和/或非突触性接触。以上结果表明,NT可通过轴轴突触和/或非突触性轴轴接触的一级传入C纤维的传入进行突触前抑制。此外,脊髓后角内还存在变性终末与含NTLI树突形成的轴树突触。 相似文献
934.
实验性高血压大鼠室旁核加压素分泌的免疫细胞化学研究 总被引:2,自引:0,他引:2
精氨酸加压素(AVP)主要是由下丘脑室旁核(PVN)和视上核(SON)的加压素能神经元合成分泌。近年来的研究表明,AVP作为一种血管活性肽与高血压的发病有关。本文采用二肾一夹法制成高血压模型。应用光、电镜技术、免疫细胞化不技术和图象分析技术对实验性高血压大鼠PVN加压素神经元进行了研究,并与SON加压素神经元及正常大鼠进行比较,研究结果表明,实验性高血压大鼠PVN和SON内AVP阳性细胞中分泌颗粒密集呈棕黄色,正常大鼠组染色浅谈。图象分析检测两组PVN和SON中AVP阳性细胞平均灰度值,所得数据分别经统计学处理,实验组和正常组AVP神经元在PVN有显著性差异。在SON也有显著性差异。但在实验组内的PVN和SON之间无显著性差异,正常大鼠组PVN和SON之间亦无显著性差异。结果表明, 高血压大鼠在血压升高时,PVN和SON内加压素神经元的分泌增强。 相似文献
935.
Masahiro Shibasaki Daiki Masukawa Kazunori Ishii Yui Yamagishi Tomohisa Mori Tsutomu Suzuki 《Journal of neurochemistry》2013,125(5):747-755
Benzodiazepines are commonly used as sedatives, sleeping aids, and anti‐anxiety drugs. However, chronic treatment with benzodiazepines is known to induce dependence, which is considered related to neuroplastic changes in the mesolimbic system. This study investigated the involvement of K+‐Cl? co‐transporter 2 (KCC2) in the sensitization to morphine‐induced hyperlocomotion after chronic treatment with zolpidem [a selective agonist of γ‐aminobutyric acid A‐type receptor (GABAAR) α1 subunit]. In this study, chronic treatment with zolpidem enhanced morphine‐induced hyperlocomotion, which is accompanied by the up‐regulation of KCC2 in the limbic forebrain. We also found that chronic treatment with zolpidem induced the down‐regulation of protein phosphatase‐1 (PP‐1) as well as the up‐regulation of phosphorylated protein kinase C γ (pPKCγ). Furthermore, PP‐1 directly associated with KCC2 and pPKCγ, whereas pPKCγ did not associate with KCC2. On the other hand, pre‐treatment with furosemide (a KCC2 inhibitor) suppressed the enhancing effects of zolpidem on morphine‐induced hyperlocomotion. These results suggest that the mesolimbic dopaminergic system could be amenable to neuroplastic change through a pPKCγ‐PP‐1‐KCC2 pathway by chronic treatment with zolpidem. 相似文献
936.
Kai Chen Chen Su Wensi Tang Yongbin Zhou Zhaoshi Xu Jun Chen Haiyan Li Ming Chen Youzhi Ma 《The Plant journal : for cell and molecular biology》2021,107(3):740-759
Drought is a critical abiotic stressor that modulates soybean yield. Drought stress drastically enhances reactive oxygen species (ROS) formation, and maintaining ROS content above a cytostatic level but below a cytotoxic level is essential for normal biology processes in plants. At present, most of the known ROS-scavenging systems are in the cytoplasm, and the mechanism of ROS regulation in the nucleus remains unclear. GmNTF2B-1 is a member of the IV subgroup in the nucleus transporter family. Its expression is localized to the roots and is stimulated by drought stress. In this study, the overexpression of GmNTF2B-1 was found to improve the drought tolerance of transgenic soybean by influencing the ROS content in plants. An oxidoreductase, GmOXR17, was identified to interact with GmNTF2B-1 in the nucleus through the yeast two-hybrid, coimmunoprecipitation and bimolecular fluorescence complementation assays. The drought tolerance of GmOXR17 transgenic soybean was similar to that of GmNTF2B-1. GmNTF2B-1 was expressed in both cytoplasm and nucleus, and GmOXR17 transferred from the cytoplasm to the nucleus under drought stress. The overexpression of GmNTF2B-1 enhanced the nuclear entry of GmOXR17, and the overexpression of GmNTF2B-1 or GmOXR17 could decrease the H2O2 content and oxidation level in the nucleus. In conclusion, the interaction between GmNTF2B-1 and GmOXR17 may enhance the nuclear entry of GmOXR17, thereby enhancing nuclear ROS scavenging to improve the drought resistance of soybean. 相似文献
937.
Jun氨基末端激酶 (JunN terminalkinase ,JNK)是一种重要的细胞信号传递者。它参与了细胞生长、分化、程序性死亡等生理过程 ,而且在调节上皮细胞运动和形态发生等方面也起着重要作用。大量研究证实 ,在果蝇Drosophila的背闭合行为 (dorsalclosure,DC)中 ,DJNK(DrosophilaJNK)的调节是关键。文章就果蝇DC的发生过程以及DJNK信号途径的研究进展作一简要的综述。 相似文献
938.
Catecholaminergic fibers in the suprachiasmatic nucleus of adult rats were investigated by use of light- and electron-microscopic immunocytochemistry. The suprachiasmatic nucleus receives a modest density of tyrosine hydroxylase-containing axons, homogeneously distributed in the nucleus and forming varicosities throughout its entire rostro-caudal extension. Immunolabeling with antibodies against dopamine showed that this catecholamine input comprises a dopaminergic component. Many tyrosine hydroxylase-positive cells were localized at the immediate periphery of the suprachiasmatic nucleus. With electron-microscopic examination, dendrites of these neurons were found within the limits of the nucleus as well as at a border zone between the suprachiasmatic nucleus proper and the optic tract where they received unlabeled synapses, providing a morphological support for a possible role of dopaminergic neurons in the integration and/or transfer of light-related signals. More than 91% of catecholaminergic axonal varicosities were found to establish morphologically defined synapses with dendrites. To investigate whether these synapses might be shared with neurons of one or both of the two main peptidergic populations of the nucleus, namely vasoactive intestinal peptide- and vasopressin-containing neurons, we carried out doublelabeling experments combining immunoperoxidase and immunogold-silver labeling. Results showed only a few cases of direct association of the catecholaminergic terminals with these peptidergic categories. In both types of dually stained sections, catecholaminergic synapses were preferentially made with unlabeled dendrites. The homogeneous distribution of tyrosine hydroxylase-immunoreactive fibers in the suprachiasmatic nucleus could therefore reflect a lack of significant catecholaminergic innervation of both vasoactive intestinal peptide- and vasopressin-synthesizing neurons. 相似文献
939.
The effect of reserpine on neuropeptide Y immunoreactive (NPY-IR) neurons in the rat hypothalamic arcuate nucleus was examined
by immunocytochemical techniques. Although only NPY-IR fibers and terminals were distributed in this nucleus in untreated
and saline treated rats, single treatment of reserpine (10 mg/kg, i.p.) visualized abundant NPY-IR neuronal cell bodies: the
increase began at 12 h of postinjection, reached its maximal level at 48 h, and returned to its normal level at 96 h. Pretreatment
of nialamide, a monoamine oxidase inhibitor, prevented these acute reserpine-induced changes, suggesting reserpine acts on
NPY neurons through monoaminergic mechanism. Chronic treatment of haloperidol (5 mg/kg, once daily for 5 days) a dopamine
receptor antagonist, could induce the similar increase of NPY immunoreactivity. However, interruption of adrenergic and serotonergic
neurotransmissions by chronic treatment of propranorol and methysergide, or chemical lesions of ascending noradrenergic and
serotononergic pathways by 6-hydroxydopamine and 5,6-dihydroxytryptamine, could not induce any immunoreactive increase of
NPY in arcuate neurons. These findings strongly suggest that reserpine-induced NPY increase occurs through dopaminergic afferents
in hypothalamic arcuate neurons.
Special issue dedicated to Dr. Kinya Kuriyama. 相似文献
940.