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91.
Peptidergic neurons of the crab,Cardisoma carnifex,in defined culture maintain characteristic morphologies under a variety of conditions 总被引:5,自引:0,他引:5
Summary Peptidergic neurons dissociated from the neurosecretory cell group, the X-organ, of adult crabs (Cardisoma carnifex) show immediate outgrowth on unconditioned plastic dishes in defined medium. Most of the neurons can be categorized as small cells, branchers or veilers. A fourth type, superlarge, found occasionally, has a soma diameter greater than 40 m and multipolar outgrowth. We report here the effects on morphology that follow alterations of the standard defined culturing conditions. The three common types of neurons are present when cells are grown in crab saline or saline with l-glutamine and glucose (saline medium). Changes of pH between 7.0 to 7.9 have no effect. Osmolarity changes cause transient varicosities in small cells. In some veilers, pits rapidly appear in the veil and then disappear within 35 min. In cultures at 26° C instead of 22° C, veilers extend processes from the initial veil in a pattern similar to branchers, and the processes of adjacent veilers sometimes form appositions. Culturing in higher [K+]o medium ([K+]o=15–110 mM; standard=11 mM) has no long-term effect, but growth is arrested by [K+]o greater than 30 mM. Cultures were also grown in media in which [Ca2+]o ranged from 0.1 M to 26 mM (standard=13 mM). Outgrowth occured from all neuronal types in all [Ca2+]o tested. Thus, the expression of different outgrowth morphologies occurs under a wide variety of culturing conditions. 相似文献
92.
Nitrosomonas europaea is capable of maintaining an anaerobic metabolism, using pyruvate as an electron donor and nitrite as an electron acceptor; utilization of nitrite depends upon supply of both pyruvate and ammonia. The role of ammonia in this reaction was not determined. N europaea also assimilates CO2 anaerobically into cell material in the presence of nitrite (0.5–1.0 mM), pyruvate and ammonia. This reaction was partially inhibited by nitrite which apparently competed with CO2 for reducing power. Anaerobic nitrite respiration is sensitive to ionophores, FCCP being the most effective.Non-standard-abbreviations TCA
trichloroacetic acid
- FCCP
carbonylcyanide-p-trifluoromethoxyphenylhydrazon 相似文献
93.
Synopsis This study tested the hypothesis that visual contact between fish may result in enhanced rates of growth in a schooling fish. Juvenile chum salmon, Oncorhynchus keta, were held singly and reared in isolation or in visual contact with conspecifics. Fish were fed at either a low (6% body weight d–1) or high (20% body weight d–1) ration for 42d. Specific rates of weight gain were 18% greater at low ration and 38% greater at high ration for fish in visual contract with conspecifics than for those held in isolation. The results demonstrate a selective advantage of visual cues associated with schooling behavior and suggest that the efficacy of growth models for schooling fishes may be enhanced by the consideration of social interactions which may facilitate growth. 相似文献
94.
Nicholas F. Hughes 《Environmental Biology of Fishes》1992,33(3):299-305
Synopsis Length-frequency data suggest Nile perch, Lates niloticus, from the Nyanza Gulf grew to a total length of 9 cm by age 118 days and 23 cm by age 287 days. A modified von Bertalanffy growth curve t = 1.35·L(1-e–K(t-t
o)) with the parameters L = 93.1, K = 0.272 and to = 0.046, is suggested to describe growth up to 5 years of age and the relationship t = 1.35·(31.96 + 7.681t) for fish aged 6 years and above. Length-weight relationships were = 0.0234·-gt2.74 for fish between 7 and 15.9 cm total length, = 0.0151·2.94 for fish between 16 and 45.9 cm total length, and = 0.0023·3.44 for fish between 46 and 120 cm total length. Male Nile perch first matured between 50 and 55 cm total length when they were probably 2 years old; female Nile perch first matured between 80 and 85 cm total length when they were probably 4 years old. Small males were common, large males were rare, with the reverse holding for females. Sex change, from male to female, is a possible explanation for this size dimorphism. 相似文献
95.
Synopsis Changes in the daily appetite and weekly growth rates of individual adult minnows,Phoxinus phoxinus, on ad libitum rations were recorded before and after they had experienced 4 or 16 days of food restriction. Feeding levels during the restriction periods were either starvation or a maintenance ration. The latter was estimated from a previously determined regression model. Water temperature was 15°C and the photoperiod 9L15D in all experiments. The mean weight of fish used ranged from 1.06 to 2.15 g. The 4 day restriction had no detectable effects on appetite or growth. After the 16 day restriction, the minnows showed hyperphagia and had increased specific growth rates and growth efficiencies compared with control fish. The compensatory increases in appetite and growth were not sustained and within three weeks had declined to levels not significantly different from those of the control fish. At the end of the experiments, there were no significant differences between the mean weights or cumulative food consumption of the restricted and control groups. The results suggest that adult minnows regulate their appetite and growth rate in relation to their previous nutritional history. 相似文献
96.
In an 18 year old Japanese larch stand, leaf characteristics such as area, weight, gross photosynthetic rate and respiration
rate were studied in order to obtain basic information on estimating canopy photosynthesis and respiration. The leaf growth
courses in area and weight from bud opening were approximated by simple logistic curves. The growth coefficient for the area
growth curve was 0.155–0.175 day−1, while that for the weight growth was 0.112–0.117 day−1. The larger growth coefficient in area growth caused the seasonal change in specific leaf area (SLA) that increased after bud opening to its peak early in May at almost 300 cm2 g−1 and then decreased until it leveled off at about 140 cm2g−1. The change inSLA indicates the possibility that leaf area growth precedes leaf thickness growth. The relationship between the coefficientsa andb of the gross photosynthetic rate (p)-light flux density (1) curve (p=bI/(1+aI)) and the mean relative light flux density (I′/I
0) at each canopy height were approximated by hyperbolic formulae:a=A/(I′/I
0)+B andb=C/(I′/I
0)+D. Leaf respiration rate was also increased with increasingI′/I
0. Seasonal change of gross photosynthetic rate and leaf respiration rate were related to mean air temperature through linear
regression on semilogarithmic co-ordinates. 相似文献
97.
98.
Summary Experiments conducted on rocky bottoms at 7–11 m depth in the San Juan Archipelago, Washington assessed effects of canopies
of understory kelps on growth of benthic suspension feeders, determined the mechanisms responsible for effects, and assessed
the influence of kelp canopies on survivorship of benthic fauna. Kelp canopics influenced growth rates of diverse suspension
feeders. At several sites the musselMytilus edulis, the barnacleBalanus glandula, and the serpulid polychaetePseudochitinopoma occidentalis grew faster on the bottom beneath kelp canopies than on nearby exposed substrata. The cheilostome bryozoanMembranipora membranacea showed a mixed response to kelp canopies, growing faster in exposed regions at one site, but faster beneath canopies at another.
There were no differences in growth of 2 other species (the cheilostome bryozoanCheilopora praelonga and the spongeMyxilla incrustans) between kelp and no-kelp treatments; however, some processes influenced by plant canopies affected their growth. Specific
mechanisms responsible for kelp effects on growth were assessed in a series of field experiments usingPseudochitinopoma, Membranipora, Cheilopora andMyxilla. Particulate deposition on the bottom, which is more intense beneath canopies, negatively affected growth of all 4 species.
Kelps also reduced rates of flow and prevented devented development of microalgal turfs beneath the canopy.Pseudochitinopoma grew faster in the weaker flows below canopies and bothCheilopora andMyxilla grew faster where there were no microalgal turfs. These other effects of kelp canopies were at least as important to growth
(in the cases ofCheilopora andMyxilla) or more important to growth (in the case ofPseudochitinopoma) than were the general, deleterious effects of higher sedimentation beneath canopies.
The lower growth rates caused by higher sedimentation beneath kelp canopies did not result in higher rates of animal mortality.
Surprisingly, kelp canopies typically did not influence mortality due to predation. For 7 of 12 taxa, mortality rates did
not differ between kelp-covered and exposed treatments. Significantly higher mortality occurred outside canopies for only
4 of 12 taxa, and for at least 2 of these 4 differences probably were not related to predation.Mytilus, a species rare at these depths, exhibited higher mortality beneath kelp canopies due to predation by crabs. Other macrophytes
in fresh and salt water, as well as some benthic animals that create complex, 3-dimensional habitats, should influence benthic
organisms and assemblages in ways analogous to the kelps acting through their effects on flow, particle transport, and shading. 相似文献
99.
Effect of stress and time for recovery on the amount of compensatory growth after grazing 总被引:7,自引:0,他引:7
Summary We tested the hypothesis that the amount of compensatory growth after defoliation is affected by the level of stress at which plants grow when defoliated and by the length of time for recovery. Growth response to defoliation went from partial compensation when plants were growing at high relative growth rates (RGR) to overcompensation when plants were more stressed and growing at low RGR. Defoliation released plants from the limitation imposed by the accumulation of old and dead tissue and this release overrode the negative effect of biomass loss. Compensatory growth resulted from a higher RGR aboveground that was not associated with a reduction in RGR belowground. Time available for recovery had a major impact on the outcome of defoliation. With a short time for recovery, RGR was decreased by defoliation because an immediate increase in net assimilation rate was overridden by a reduction in the ratio of leaf area to plant weight. After defoliation, this ratio increased quickly due to a larger allocation to leaf growth and lower leaf specific weights, resulting in higher RGR. We conclude that the compensatory response to grazing depends on the type and level of stress limiting growth. Allocation and physiological responses to stress may positively or negatively affect the response to grazing and, simultaneously, grazing may alleviate or aggravate the effects of different types of stress. 相似文献
100.
Summary
Gossypium hirsutum L. var. Delta Pine 61 was cultivated in controlled-environment chambers at 1000–1100 mol photosynthetically active photons m-2 s-1 (medium photon flux density) and at 1800–2000 mol photons m-2 s-1 (high photon flux density), respectively. Air temperatures ranged from 20° to 34°C during 12-h light periods, whereas during dark periods temperature was 25° C in all experiments. As the leaf temperature decreased from about 33° to 27° C, marked reductions in dry matter production, leaf chlorophyll content and photosynthetic capacity occurred in plants growing under high light conditions, to values far below those in plants growing at 27° C and medium photon flux densities. The results show that slightly suboptimum temperatures, well above the so-called chilling range (0–12° C), greatly reduce dry matter production in cotton when combined with high photon flux densities equivalent to full sunlight.Abbreviations DW
dry weight
-
F
v
variable fluorescence yield
-
F
M
maximum fluorescence yield
- PFD
photon flux density (400–700 nm) 相似文献