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141.
Claus Braestrup† Mogens Nielsen† Povl Krogsgaard-Larsen‡ 《Journal of neurochemistry》1986,47(3):691-696
[3H]Strychnine binding to rat pons + medulla membranes was used as a measure of glycine receptors or glycine receptor-coupled chloride channels in vitro. A series of compounds structurally related to 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol (THIP), which previously were shown to antagonize glycine responses in cat spinal cord, inhibited [3H]strychnine binding in micromolar concentrations. The most potent of these glycine antagonists, 5,6,7,8-tetrahydro-4H-isoxazolo[3,4-d]azepin-3-ol (iso-THAZ), was also the most potent inhibitor of [3H]strychnine binding, with a Ki of 1,400 nM. The Ki value for strychnine was 7.0 nM, whereas the Ki value for the mixed gamma-aminobutyric acid (GABA)/glycine antagonist 3 alpha-hydroxy-16-imino-5 beta-17-aza-androstan-11-one (RU 5135) was only 4.6 nM. Sodium chloride (1,000 mM) enhanced the affinity of strychnine, brucine, isostrychnine, and the nonselective GABA antagonist pitrazepin for [3H]strychnine binding sites, whereas the affinities of glycine, beta-alanine, and taurine were reduced. These sodium chloride shifts, however, were not predictive of antagonist or agonist properties, since the sodium chloride shift for the glycine antagonist iso-THAZ and of the other THIP-related antagonists were similar to those of the glycine-like agonists. The various sodium chloride shifts show that different groups of ligands bind to glycine receptor sites in different ways. 相似文献
142.
神农香菊干花净油成分的研究 总被引:5,自引:2,他引:3
王国亮;王金凤;张红旗;贾卫疆;袁焱明 《武汉植物学研究》1986,4(1):65-68
神农香菊全草提取的香浸膏香气浓郁独特,可用于调配多种高档化妆用香精,也可直接用于饮料中,在香精香料工业中具有较大的实用价值。本文首次报告了我们用毛细管气相色谱仪和色谱/质谱/计算机联用分析仪,对神农香菊干花净油成分进行分析的初步结果。鉴定出的已知成分包括脂肪族类,含氧或非含氧的单萜及倍半萜类化合物32个。 相似文献
143.
Ieharu Hishinuma Tetsuro Ishii Hiroyuki Watanabe Shiro Bannai 《In vitro cellular & developmental biology. Plant》1986,22(3):127-134
Summary Mouse lymphoma L1210 cells maintained in vitro at a high cell density for a certain time period adapted themselves to the
in vitro environment and were able to grow indefinitely. From these adapted cells, more than 30 clones were isolated. They
all had much higher activity to take up cystine than the original L1210 cells, supporting a previous view that the deficiency
of the cystine uptake limits the survival and growth of L1210 cells in vitro. The cystine uptake of one cloned cell line was
characterized. The enhanced uptake of cystine in these cells was mainly mediated by a Na+-independent, saturable system and was potently inhibited by glutamate and some other anionic amino acids, but less by aspartate.
Such activity of cystine uptake was not observed in the original L1210 cells. The results suggest that, upon adaptation in
vitro, L1210 cells acquire a new cystine transport activity necessary for survival and growth in vitro. 相似文献
144.
Summary We have examined the radiolysis of an O2-free aqueous solution of glycine at absorbed doses of60Co gamma-radiation of up to 20 Mrad. At least 20 compounds are formed during radiolysis, among them several amino acids, an oligoamine, and the nitrogen-free polymers (Mw28,000 daltons). When dicyandiamide is present in the solution of glycine, various nitrogen-containing products, including some polymers (Mw12,000 daltons), are synthesized along with radiolytic products of glycine; polyglycines are not formed. We have determined the radiation-chemical yields of radiolytic-product formation and of decomposition of glycine, and have considered possible free-radical reactions leading to the radiation-induced changes observed. 相似文献
145.
Cholecystokinin receptor mediated hydrolysis of inositol phospholipids in guinea pig gastric glands 总被引:1,自引:0,他引:1
CCK-octapeptide (CCK-8) (EC50 = 0.5 nM), in the presence of Li+, increased 3H-inositol phosphate (IP) accumulation in guinea pig gastric glands prelabeled with 3H-inositol. CCK-8 desulfate, human gastrin I and pentagastrin were much less potent than CCK-8. Antagonists of CCK receptors such as proglumide, dibutyryl-c-GMP and CBZ-Tyr (SO3H)-Met-Gly-Trp-Met-AspNH2 shifted the CCK dose response curve to the right. However, histamine (H1 and H2), cholinergic, substance P and alpha- and beta-adrenergic receptor antagonists had no effect on 3H-IP accumulation induced by CCK. The results suggest that CCK receptor activation in gastric glands leads to an enhanced breakdown of inositol phospholipids which may relate to calcium mobilization and pepsinogen secretion. 相似文献
146.
Pollen flow, population composition, and the adaptive significance of distyly in Linum tenuifolium L. (Linaceae) 总被引:1,自引:0,他引:1
MARC S. NICHOLLS 《Biological journal of the Linnean Society. Linnean Society of London》1985,25(3):235-242
The function of distyly as a device promoting inter-morph pollination was proposed by Darwin over a century ago, though only recently have experimental studies been undertaken to test this hypothesis. These studies are unrealistic since they assume regular spatial distribution of morphs within a population and fail to consider intra-flower pollination in assessing distyly's effects. Pollen flow in two populations of Linum tenuifolium L. was investigated using a fluorescent-dye marker technique. One population was dimorphic and self-incompatible; the other was monomorphic and self-compatible. In both populations pollen dispersal was leptokurtic with over 75% of grains detected within 5 m from the source. Intra-flower pollinations comprised a large proportion of individual stigma loads (42% for the dimorphic form; 49% for the monomorphic form). Total stigmatic loads for the dimorphic population showed equal proportions of pin and thrum pollen on both long-style and short-style stigmas, though variation in individual loads was marked. Values ranging from 34.2 to 61.5% legitimate pollen were recorded. The irregular distribution of morphs observed at the dimorphic site will reduce the efficiency of heterostyly as a device promoting intermorph pollination. The reduction in intra-flower pollination suggests adaptive significance of this floral dimorphism in increasing the efficiency of pollen dispersal to the individual. 相似文献
147.
David A. Priestley Brenda G. Werner A. Carl Leopold Murray B. McBride 《Physiologia plantarum》1985,64(1):88-94
In view of their possible role in oxidative deterioration of seeds and pollen, organic free radicals were measured by electron spin resonance in embryonic axes and cotyledons of soybean [ Glycine max (L.) Merr], embryo and endosperm fractions of corn [ Zea mays L.] and pollen of cattail [ Typha latifolia L.]. A pronounced decline in the radical signal ensued when hydration increased above about 7% (wet weight basis) in both the seed materials and in pollen. Moderate hydration of the soybean axis followed by drying led to a small decrease in organic free radicals compared to untreated material, especially if the desiccation step was performed under nitrogen. In a comparison of soybeans of various ages under normal storage, organic free radical levels in the axis showed little or no increase with age. In marked contrast, over 5 days of accelerated aging at 40°C and near-saturating humidity, organic radical levels approximately doubled in the axis. This pronounced increase in free radical content was not associated with a decrease in the proportion of polyunsaturated fatty acids. The data suggest that hydration of seed and pollen causes a release of free radicals from the trapped state. 相似文献
148.
Summary Information is limited on soil contamination of leaves from field-grown row crops, especially with respect to aluminum (Al) analyses. The objective of this study was to determine the influence of washing leaf samples with either deionized water or detergent solution on elemental analyses for several agronomic crop plants. The crop plants sampled were corn (Zea mays L.), soybean (Glycine max L. Merr.), grain sorghum (Sorghum bicolor L. Moench), and wheat (Triticum aestivum L.). The crops were grown on a range of soil types, soil pH values, and tillage practices. Samples of upper leaves and lower leaves were collected separately. The samples were either not washed, washed with deionized water, or washed with detergent solution. After drying, grinding, and digesting, the samples were analyzed for Al, nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu). For all crop plants and conditions studied, there was no effect on measured N, P, K, Ca, Mg, Mn, Zn, or Cu concentrations, but measured Al and Fe concentrations were influenced by washing. In general, washing had a greater effect on Al analyses than on Fe analyses. Soybean samples were most affected by washing, while wheat samples seemed to be least affected. The results reflected greater contamination of lower leaves than upper leaves. Decontamination procedures appear necessary prior to Al and Fe analyses of field-grown crop plants. 相似文献
149.
Summary Glycine betaine is readily accumulated in wheat (Triticum aestivum cv. Inia) shoots during periods of salinity stress. The ability of the plant to utilize betaine as a source of nitrogen remains unresolved. We, therefore, conducted solution culture experiments in a greenhouse to test the hypothesis that betaine is degraded in wheat shoots under conditions of severe nitrogen deficiency. Betaine concentrations increased in continuously salt stressed plants for only 17 days after salinity was imposed. After this period, concentrations (dry weight basis) decreased steadily until plants died 32 days later. Decreases in betaine concentration were also observed in treatments where salinity stress was removed. The rate of decrease in concentration was greatest in the N-free treatment. These decreases in betaine concentration were the result of dilution by plant growth. Betaine contents (mol shoot–1) remained unchanged after removal of substrate nitrate. Therefore our results support the hypothesis that betaine is a stable end product of metabolism. 相似文献
150.
Summary A plant root observation chamber (rhizotron) was designed to examine soil-grown roots under a stereomicroscope and to sample roots and soil during the growth period of a pot study. The mini-rhizotrons are inexpensive to construct and are suitable for replicated, multitreatment experiments. Illustrative data on root hair and lateral development are presented for seedlings of four crop species.Vermont Agric. Exp. Stn. Journal Article No 572. 相似文献