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141.
wo laboratory strains of Glossina morsitans centralis originating from different fly-belts (one from Singida, in Tanzania, and the other from Mumbwa, in Zambia) were compared with respect to vectorial competence for pathogenic Trypanosoma species, genetic variation and inter-colony fertility. The vectorial competence of G.m.centralis of Tanzanian origin for Trypanosoma vivax and T.congolense is similar to, whereas for T.brucei brucei it is lower than the colony of Zambian origin. Nevertheless, these two laboratory strains of G.m.centralis showed levels of susceptibility to the three pathogenic Trypanosoma species which were much greater than previously observed in laboratory colonies of other Glossina species. Electrophoresis of fifteen enzymes revealed that the two colonies differ significantly in allele frequencies at only three loci that are relatively close together on one of the autosomes. Hybridization experiments revealed that G.m.centralis from the two fly-belts are consubspecific. 相似文献
142.
The flies of genus Glossina (Diptera: Glossinidae) are an important vector of African trypanosomiases which cause diseases in humans and animals. The ribosomal DNA Internal Transcribed Spacer-2 (ITS-2) region sequences from different Glossina species were PCR-amplified and analyzed in order to construct a molecular phylogeny for genus Glossina. Trees generated by parsimony confirmed the monophyletic taxonomic placement of genus Glossina where fusca group species formed the deepest branch followed by morsitans and palpalis groups, respectively. The placement of Glossina austeni by both the traditional morphological and biochemical criteria has been controversial. Results presented here, based on ITS-2 locus sequence analysis, suggest that Glossina austeni can be placed into a separate subgenerus which forms a sister-group relationship with the morsitans group species. 相似文献
143.
Development of a low-cost tsetse trap and odour baits for Glossina pallidipes and G.longipennis in Kenya 总被引:1,自引:0,他引:1
Experiments were carried out to improve the NG2B tsetse trap (Brightwell et al., 1987), baited with acetone and cow urine, for use by rural communities to control G.pallidipes Austen and G.longipennis Corti. Modifications included a lower dose rate of acetone, a new cage design and raising the trap about 15-20 cm. Research on different trap cone materials showed that the degree of light transmission of the netting, rather than its colour, was the crucial factor affecting the catch of G.pallidipes. Adding an additional metre of blue cloth to one side of the trap increased catches of females of both species by about 60%. Traps baited with synthetic phenols yielded similar numbers of G.pallidipes and significantly more G.longipennis than those baited with natural cow urine. The latter difference was not apparent when octenol was also used, so cow urine was retained as one of the odour baits in preference to the imported phenols. Although octenol increased catches of G.pallidipes by only about 30%, catches of G.longipennis were increased 2-4-fold, making it a very useful attractant for the latter species. The cost of the trap/odour-bait system was estimated to be US$8.5 per unit per annum. The economics of this method of tsetse control are discussed. 相似文献
144.
Orientation of tsetse flies to wind, within and outside host odour plumes in the field 总被引:1,自引:0,他引:1
Abstract Free-flying wild tsetse flies ( Glossina pallidipes Aust. and G. m. morsitans Westw.) were video recorded in Zimbabwe as they flew within an artificial host odour plume at 3, 7 or 15 m from the source, or in no odour, with and without a 0.75 m2 vertical, black visual target present aligned with the wind. With no visual target present, flights in odour were strongly biased upwind, and in the absence of odour strongly biased downwind. With the target present, between 16% and 40% of the upwind approaching flies responded visually as they passed the target, by circling it, in proportion to the proximity of the source (taken to be proportional to the mean odour concentration). Crosswind approaching flies (for whom the target will have been visible for some metres away) circled more frequently (34–56%), but without obvious correlation with the odour concentration. Circling flies also responded orthokinetically, by slowing down as they passed the target. The departure directions relative to the wind of flies leaving the target were significantly affected by the odour concentration. At 3 m they left the target in all directions, except possibly avoiding due upwind. At 7 m they left with an obliquely upwind bias, but at 15 m and also in no odour, they left with a strong crosswind bias. 相似文献
145.
Changes in temperature, not photoperiod, control the pattern of adult eclosion in the tsetse, Glossina morsitans 总被引:1,自引:0,他引:1
Abstract. The timing of adult eclosion in tsetse, an event that normally occurs in mid-afternoon, is regulated by the daily cycle of temperature elevation. If a temperature cycle is maintained, the rhythm of eclosion persists under continuous light or continuous darkness. Artificially shifting the temperature peak to the scotophase results in a concomitant shift in the eclosion pattern. Daily temperature variations as small as 0.4°C are sufficient to establish the rhythm. Eclosion activity tracks the temperature peak, even if the pulses are of short duration (4 h) or with irregular frequencies of 12 or 36 h. The temperature-induced rhythm offers a simple mechanism for separating females and males. Individuals that pupariate on the same day eclose as adults over a 4–5 day period at 25°C, and in such collections, females are the first to eclose. This distinction makes it possible to collect samples of predominately one sex, a feature that may facilitate the collecting of males for use in the sterile-male technique. 相似文献
146.
The Polymerase Chain Reaction (PCR) technique was used for the identification of natural trypanosome infections in Glossina longipalpis (Diptera: Glossinidae) in Côte d'Ivoire. A total number of 139 flies were examined microscopically for the presence of trypanosomes. Out of them 50 were detected positive and were subsequently prepared for the PCR using primers specific for Trypanosoma (Nannomonas) congolense of Savannah, Riverine-Forest, Kilifi, and Tsavo types, T. (N.) simiae, T. (Duttonella) vivax and Trypanozoon. Almost 90% of the infections detected by the PCR were attributed to Nannomonas, especially T. congolense Savannah and Riverine-Forest types, with many infections in which both of these two types were present T. simiae and T. vivax were also detected in some flies. The sequence specificity of the PCR products was confirmed by hybridization with parasite-type specific DNA probes. Differences between parasitological and PCR results are discussed. 相似文献
147.
Glossina morsitans and G. tachinoides were successfully infected with 2 isolates of Trypanosoma vivax which had an inherent property for serial maintenance in mice. The infection rate in the flies was relatively high. Cyclical transmission of these isolates from sheep to sheep and from goat to goat was achieved and did not affect the property of the isolates to infect mice. Mice were not apparently suitable for direct fly transmission experiments of T. vivax. 相似文献
148.
J.W. Hargrove 《Entomologia Experimentalis et Applicata》1999,92(1):89-99
Between November 1988 and July 1995 five technicians carried out ovarian dissections on 16,013 Glossina morsitans morsitans Westwood and 123,848 G. pallidipes Austen captured at Rekomitjie Research Station, Zambezi Valley, Zimbabwe. The ovarian age and uterine content were recorded, as were the lengths (l1, l2 and lu) of the largest and second largest oocyte, and of any uterine inclusion. Major abnormalities and abnormal spermathecal contents were found in <0.1% of all flies dissected. Apparent abortions rates varied significantly between dissectors and occurred at frequencies of 0.8–4.5% in G. m. morsitans and 0.3–2.8% in G. pallidipes. The lowest estimates give the best picture of the field situation. Abortion rates were higher in flies caught on electric nets than in trapped flies where the rate was only 0.15%, indicating that reproductive losses are negligible for most of the year at Rekomitjie. The rates did, however, increase to >2% when mean temperatures exceeded 27°C and flies were captured in artificial refuges. There was little effect of ovarian age on the abortion rate, but the frequency of empty uteri declined markedly with age – with a suggestion, however, that it might increase again in the oldest flies. A knowledge of the rates of reproductive loss is important for the construction of realistic models of the dynamics of tsetse populations. 相似文献
149.
Intraspecific variability in natural populations of Glossina palpalis gambiensis from West Africa, revealed by genetic and morphometric analyses 总被引:2,自引:0,他引:2
Solano P de La Rocque S Cuisance D Geoffroy B De Meeus T Cuny G DuVallet G 《Medical and veterinary entomology》1999,13(4):401-407
Glossina palpalis gambiensis Vanderplank (Diptera: Glossinidae) from West Africa (Senegal and Burkina Faso) were analysed for microsatellite DNA polymorphisms and size of the wings. In the overall sample a strong heterozygote deficiency was found at two polymorphic microsatellite loci. It led to a highly significant value of Fis (within-sample heterozygote deficit) in the western zone of Sideradougou area in Burkina Faso. Genetic differentiation was significant on a macrogeographic scale, i.e. between tsetse coming from Senegal and Burkina Faso. Wing measures also differed between these two countries; flies from Senegal appeared to be smaller. Microsatellite loci further allowed differentiation of populations of G. palpalis gambiensis trapped on the same hydrographic network a few kilometres apart. The results are interpreted as indicating that further investigations will allow the study of genetic variability of tsetse flies in relation to the dynamics of transmission of human and animal trypanosomoses. 相似文献
150.
Monitoring tsetse fly populations.:II. The effect of climate on trap catches of Glossina pallidipes 总被引:1,自引:0,他引:1
BRIAN WILLIAMS ROBERT BRIGHTWELL ROBERT DRANSFIELD 《Medical and veterinary entomology》1990,4(2):181-193
In Part I it was shown that the sampling distribution of trap catches of tsetse flies, Glossina pallidipes Austen, at Nguruman, Kenya, using unbaited biconical traps follows a Poisson distribution. In this paper we examine the effect of humidity and temperature on day-to-day and seasonal variations in the trap catches. It is shown that the seasonal variation is significantly correlated with maximum daily temperature, the catches increasing with temperature when the maximum temperature is below 34 degrees C and decreasing with temperature when it is above 34 degrees C. The correlation between trap catches and relative humidity is not as good as the correlation with the maximum temperature, and the two together do not improve the fit to the trap catches. The day-to-day variation is significantly greater than the intrinsic variation due to the stochastic nature of the sampling process and for some traps it is correlated with temperature and humidity. An autoregressive model gives a half-life for the decay of departures from the mean of about 1 day and it is suggested that this indicates the movement of flies in response to animal movement or to climatic factors other than temperature or humidity. After removing the temperature dependent part of the seasonal variation and the autoregressive component of the data, the male and female catches are still significantly correlated. 相似文献