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61.
62.
为了解复旦大学附属华东医院长期住院老年患者感染的鲍曼不动杆菌的耐药性特征,本研究收集了2011年9月~2012年8月临床分离自老年病区长期住院患者的52株鲍曼不动杆菌,分析菌株的耐药性、产酶类型、生物膜形成能力及耐药基因的携带情况。结果显示,菌株主要分布在呼吸重症监护室,分离的标本以痰标本为主,占86.54%。52株鲍曼不动杆菌中,多重耐药菌株最高,占76.92%。对头孢哌酮/舒巴坦耐药率最低,为30.77%,对其余抗菌药物耐药率均在50%以上。产酶阳性率为82.69%,其中产其他水解酶的菌株最多,占63.46%。生物膜形成的检出率为5.77%。6株广泛耐药菌株共检出9种耐药基因。结果提示,从老年病区长期住院患者分离的鲍曼不动杆菌耐药现状严重。因此,医院感染控制应以呼吸重症监护室为重点,同时加强对生物膜阳性菌株所分布病区的耐药性监测和消毒隔离,以预防和控制耐药菌的产生和传播。 相似文献
63.
Joo Sang Lee Nishanth Ulhas Nair Gal Dinstag Lesley Chapman Youngmin Chung Kun Wang Sanju Sinha Hongui Cha Dasol Kim Alexander V. Schperberg Ajay Srinivasan Vladimir Lazar Eitan Rubin Sohyun Hwang Raanan Berger Tuvik Beker Ze’ev Ronai Sridhar Hannenhalli Eytan Ruppin 《Cell》2021,184(9):2487-2502.e13
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64.
随着社会经济的发展和人们生活水平的提高,人们的健康需求指数越来越高,法律意识也越来越强。与此同时,由医疗事故等原因导致的医疗纠纷案件正呈逐年上升趋势。有效地解决医疗纠纷,有助于建立和谐的医患关系,而和谐的医患关系是构建和谐社会一个主要的组成部分。本文在社会学视角下,运用达伦多夫和科塞的社会冲突理论来分析当前的医疗纠纷,从而促进医疗纠纷的解决。 相似文献
65.
S. Manek 《Cytopathology》2012,23(3):146-149
S. Manek The pathology clinic – pathologists should see patients This invited review describes why and how a pathologist should talk to patients in order to enhance the patient care pathway. The pathologist–patient interaction should become a natural extension to multidisciplinary team decision making, and also become the forum in which patients are helped to understand important aspects of their conditions and the pathological basis for their treatment plans. There is a vast amount of information available through the internet and to digest this can be a difficult process for a patient who is already having to cope with a medical condition. The pathologist is often best placed to sieve through this information and offer the patient the relevant detail necessary to understand the condition and the management pathway. Pathologists can provide up‐to‐date, simple information about malignant and even certain significant benign conditions, and they can do this with the help of several pictorial tools. In this way, the pathologist becomes an even more active member of a clinical team and helps both clinicians and patients to deal with illnesses in a novel way hitherto not considered. 相似文献
66.
DC‐CIK cells derived from ovarian cancer patient menstrual blood activate the TNFR1‐ASK1‐AIP1 pathway to kill autologous ovarian cancer stem cells
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Wenxing Qin Ying Xiong Juan Chen Yongyi Huang Te Liu 《Journal of cellular and molecular medicine》2018,22(7):3364-3376
Ovarian cancer stem cells (OCSCs) are highly carcinogenic and have very strong resistance to traditional chemotherapeutic drugs; therefore, they are an important factor in ovarian cancer metastasis and recurrence. It has been reported that dendritic cell (DC)‐cytokine‐induced killer (CIK) cells have significant killing effects on all cancer cells across many systems including the blood, digestive, respiratory, urinary and reproductive systems. However, whether DC‐CIK cells can selectively kill OCSCs is currently unclear. In this study, we collected ovarian cancer patient menstrual blood (OCPMB) samples to acquire mononuclear cells and isolated DC‐CIK cells in vitro. In addition, autologous CD44+/CD133+ OCSCs were isolated and used as target cells. The experimental results showed that when DC‐CIK cells and OCSCs were mixed and cultured in vitro at ratios of 5:1, 10:1 and 50:1, the DC‐CIK cells killed significant amounts of OCSCs, inhibited their invasion in vitro and promoted their apoptosis. The qPCR and Western blot results showed that DC‐CIK cells stimulated high expression levels and phosphorylation of TNFR1, ASK1, AIP1 and JNK in OCSCs through the release of TNF‐α. After the endogenous TNFR1 gene was knocked out in OCSCs using the CRISPR/Cas9 technology, the killing function of DC‐CIK cells on target OCSCs was significantly attenuated. The results of the analyses of clinical samples suggested that the TNFR1 expression level was negatively correlated with ovarian cancer stage and prognosis. Therefore, we innovatively confirmed that DC‐CIK cells derived from OCPMB could secret TNF‐α to activate the expression of the TNFR1‐ASK1‐AIP1‐JNK pathway in OCSCs and kill autologous OCSCs. 相似文献
67.
Federico Gordo Cristina Castro Inés Torrejón Sonia Bartolomé Francisco Coca Ana Abella 《Revista espa?ola de geriatría y gerontología》2018,53(4):213-216
Objective
To assess the association of previous functional status in elderly patients admitted to the ICU, estimated by the Barthel and Short Form-Late Life Function and Disability instrument scales, and the relationship with prognosis and functional capacity at hospital discharge.Material and methods
Observational prospective study of ICU-admitted patients older than 74 years, with a length of stay greater than 48 hours. Demographic data, social background, comorbidities, disability questionnaire (Barthel, Short Form-Late Life Function and Disability instrument), main diagnosis and severity (SAPS 3) on ICU admission were recorded. Factors associated with mortality or poor functional status at hospital discharge (Barthel Index less than 35) were established by multivariate analysis.Results
During the study period, 219 elderly patients were admitted in ICU, of whom 129 (15%) had an ICU length of stay greater than 48 hours. The median age was 80 years (77-83), with 52% women. Main diagnoses on admission included ischaemic heart disease (19%), another medical diagnosis (38%), and surgical procedure (43%). A Barthel score <36 (median 95, 85-100) was observed in 3% of the patients on admission. The median ICU length of stay was 5 days (4-8). ICU mortality was 6% (hospital mortality: 10%). On hospital discharge, 7% had severe dependence (Barthel <36). In this population, factors independently associated with mortality or poor functional status at hospital discharge were the pre-admission functional status, based on Short Form-Late Life Function and Disability instrument (OR 0.95, 95% CI, 0.91 to 0.98), and the severity on admission assessed by SAPS 3 (OR 1.10, 95% CI, 1.02 to 1.18), p=.0007.Conclusions
In elderly patients requiring ICU admission, a higher SAPS 3 score and functional impairment on admission were associated with mortality or severe dependence upon discharge. 相似文献68.
Rafael Bielza Paola Fuentes David Blanco Díaz Ricardo Vicente Moreno Estefanía Arias Marta Neira Ana M. Birghilescu Jorge Sanjurjo Javier Escalera David Sanz-Rosa Israel J. Thuissard Jorge F. Gómez Cerezo 《Revista espa?ola de geriatría y gerontología》2018,53(3):121-127
Introduction
The incidence of clinical complications in hip fracture (HF) patients is high and variable due to their heterogeneous nature. The aim of the study was to assess the clinical complications and their associated factors in HF patients admitted to the Orthopaedic Geriatric Unit of a 283 bed University Hospital. An average of 200 HF patients is attended yearly.Material and methods
A prospective, observational and analytical study was conducted on 383 consecutive patients admitted to the unit during the years 2013 and 2014. Clinical complications were defined according to recommendations supported by the AOTrauma Network (International Network of Traumatologists for the Study of Osteosynthesis).Results
A total of 273 patients (71.28%) showed some clinical complication. The main ones were, delirium (55.4%), renal failure (15.4%), and cardiac complications (12.3%). An ASA III-IV score of OR = 1.962 (95% CI; 1.040-3.704, P=.038), lower Barthel index at discharge (b = -3.572, 95% CI -0.866 to -0.104, P=.01), the increase in pre-operative stay (OR = 1.165, 95% CI 1.050-1.294, P=.004) and an increased length of stay (b = 2.663, 95% CI 3.522-0.325; P<.001) were factors associated with clinical complications.Conclusions
Delirium, renal failure, and cardiac complications were the most frequent complications according the new recommendations. An ASA III-IV score, worse functional status at discharge, prolonged pre-operative period, and increased length of stay, were risk factors associated with clinical complications. Cardiac, pulmonary, and gastrointestinal complications were the main causes of mortality in the unit. 相似文献69.
Summary Following the characterization of a hexadecapeptide,-endorphin, melanotropin-potentiating factor (MPF) was isolated from the filtrate of uremic patients suffering from melanosis and carbohydrate intolerance. The structure of MPF has been determined on the bases of chemical and physicochemical examinations which included HPLC, Edman sequence analysis combined analysis of the amino acid composition and FAB-MS analysis. An accumulation of MPF might be a cause of melanosis in uremic patients.Abbreviations Et3N
triethylamine
- TFA
trifluoroacetic acid
- HF
hydrogen fluoride
- CH3CN
acetonitrile
- DCC
dicyclohexyl-carbidimide
- HOBT
N-hydroxybenzotriazole
- OBzl
benzyl ester
- Boc
tert-butoxycarbonyl
- Z
bebzyloxycarbonyl
- EtOH
ethanol
- ECUM
extracorporeal ultrafiltration method
- FAB-MS
fast atom bombardment spectrometry
- HPLC
high-performance liquid chromatography
- TLC
thin-layer chromatography
- AcOH
acetic acid
- AP-M
aminopeptidase-M
- PTH
phenylthiohydantoin 相似文献
70.
用酶组织化学方法探讨经支气管纤维镜(简称纤支镜)活检组织,对肺癌的早期诊断。包括一氧化氮合酶(NOS)、细胞色素氧化酶(CCO)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、酸性磷酸酶(ACP)等。酶组织化学反应结果显示为肺癌组病人NOS、LDH、CCO、ACP的活性显著增高,分别为强阳性(),少数呈最强阳性();而SOH活性为弱阳性(+),较正常人组显著下降。可疑组病人SDH活性增高呈强阳性()或最强阳性(),但NOS、LDH、ACP的活性较肺癌组下降,分别为弱阳性(+)或中等阳性()。上述结果提示:NOS等活性的变化,可作为提高肺癌早期诊断的重要指标之一。 相似文献