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91.
G. Binelli L. Gianfranceschi M. E. Pè G. Taramino C. Busso J. Stenhouse E. Ottaviano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(1-2):10-16
Summary Densely saturated genetic maps of neutral genetic markers are a prerequisite either for plant breeding programs to improve quantitative traits in crops or for evolutionary studies. cDNA and genomic clones from maize were utilized to initiate the construction of a RFLP linkage map in Sorghum bicolor. To this purpose, an F2 population was produced from starting parental lines IS 18729 (USA) and IS 24756 (Nigeria) that were differentiated with regard to many morphological and agronomical traits. A total of 159 maize clones were hybridized to the genomic DNA of the two parents in order to detect polymorphism: 154 probes hybridized to sorghum and 58 out of these were polymorphic. In almost all of the cases hybridization patterns were similar between maize and sorghum. The analysis of the segregation of 35 polymorphic clones in an F2 population of 149 individuals yielded five linkage groups. The three principal ones recall regions of maize chromosomes 1, 3 and 5: in general, colinearity was maintained. A possible inversion, involving a long region of maize chromosome 3, was detected. Simulations were also performed to empirically obtain a value for the lowest number of individuals of the F2 population needed to obtain the same linkage data.Prof. E. Ottaviano, to whom this paper is dedicated, suddenly died on June 7th, 1991 相似文献
92.
G. Melz R. Schlegel V. Thiele 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(1):33-45
Communicated by G. Wenzel 相似文献
93.
Estimates of marker-associated QTL effects in Monte Carlo backcross generations using multiple regression 总被引:1,自引:0,他引:1
J. Moreno-Gonzalez 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(4):423-434
Summary The decision of whether or not to use QTLassociated markers in breeding programs needs further information about the magnitude of the additive and dominance effects that can be estimated. The objectives of this paper are (1) to apply some of the Moreno-Gonzalez (1993) genetic models to backcross simulation data generated by the Monte Carlo method, and (2) to get simulation information about the number of testing progenies and mapping density in relation to the magnitude of gene effect estimates. Results of the Monte Carlo study show that the stepwise regression analysis was able to detect relatively small additive and dominance effects when the QTL are independently segregating. When testing selfed families derived from backcross individuals, dominance effects had a larger error standard deviation and were estimated at a lower frequency. Linked QTL require a higher marker mapping density on the genome and a larger number of progenies to detect small genetic effects. Reduction of the environmental error variance by evaluating selfed backcross families in replicate experiments increased the power of the test. Expressions of the number of progenies for detecting significant additive effects were developed for some genetic situations. The ratio of the within-backcross genetic variance to the square of a gene effect estimate is a function of the number of progenies, the heritability of the trait, the marker map density and the portion of the genetic variance explained by the model. Different values (from 0 to 1) assigned to (relative position of the QTL in the marker segment) did not cause a large shift in the residual mean square of the model. 相似文献
94.
E. Nevo J. W. Snape B. Lavie A. Beiles 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(1-2):209-216
Summary We demonstrate that the scores and frequencies of chlortoluron (CT) and metoxuron (MX) resistance and susceptible phenotypes of wild emmer wheat, Triticum dicoccoides, are correlated with ecological factors and allozyme markers. Some isozyme markers located on chromosome 6B (e.g. Adh,Est-4 and Got), which also harbours the CT and MX resistance gene, provide good genetic markers for herbicide resistance breeding. Significant correlations between herbicide and photosynthetic characters suggest that the evolution of herbicide resistance polymorphisms may be related to the process of photosynthesis in nature and predated domestication of cultivated wheat. 相似文献
95.
P. A. Anderson G. J. Lawrence A. Pryor 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(7-8):845-850
Summary Random cDNA sequences synthesized from poly A+ RNA extracted from germinated urediospores of the flax rust fungus, Melampsora lini, were used as probes to detect restriction fragment length polymorphisms (RFLPs) in three races of M. lini originating from cultivated flax, Linum usitatissimum, and one race originating from Australian native flax, L. marginale. Fourteen out of 22 probes tested detected RFLPs in the three races from cultivated flax while 19 of the probes detected polymorphisms between these three races and the race from L. marginale. The segregation of seven RFLPs was determined in a family of 19 F2 progeny derived from a cross between two of the rust races. With six of these the inheritance was consistent, in each case, with the segregation of alleles at a single locus. Inheritance of the seventh was unusual and an explanation involving two loci with null alleles at each was proposed. No linkage was detected between any of the RFLP loci and nine unlinked loci specifying avirulence. 相似文献
96.
F M Salzano F L Black S M Callegari-Jacques S E Santos T A Weimer M A Mestriner R R Kubo J P Pandey M H Hutz 《American journal of physical anthropology》1991,85(1):51-60
Data on 31 genetic systems were obtained for 421 individuals belonging to the Arara, Araweté, Mundurucu, and Jamamadi tribes of northern Brazil. The Jamamadi depart farthest, and the Mundurucu least, from South American Indian averages. These data are analyzed together with those of 24 other Amazonian groups. Genetic distances and corresponding dendrograms indicate a cluster of 14 related tribes living north of the Amazon river. These genetic results show only a modest correlation with linguistic and geographic relationships among these groups. 相似文献
97.
Th. Geburek G. Von Wuehlisch H.-J. Muhs 《Journal of Zoological Systematics and Evolutionary Research》1991,29(1):66-72
Eleven isozyme systems were investigated in wild boar (Sus scrofa ferus L.) by means of horizontal starch gel-electrophoresis using liver and kidney extracts. Samples of 103 specimen that originated from three adjunct localities from Rhineland-Palatinate (Fed. Rep. Germany) were analysed. AAT, ACO, GDH, GPDH, LDH, ME, MPI, MR, PGDH, and PGI were invariant. Genetic polymorphism is described for PGM. The genetic polymorphism at Pgm2 is explained by a biallelic model. Observed genotypic structure did not differ significantly from Hardy-Wein-berg-proportions at this gene locus. The degree of heterozygosity is 58,3 % and Wright's Fixation Index F =–0.166 at Pgm2. The allelic structure at this gene locus differed to a great extent from that found in domestic swine, i. e. in domestic swine the faster migrating Pgm2 allozyme was only found in relative low frequencies compared to wild boar. The genetic polymorphisms were moderate in the wild boar population compared to electrophoretic data of other animal species. 相似文献
98.
Five polymorphic enzymatic loci were studied in 30 populations of Quercus ilex distributed from Crete to North Africa. A high within-population genetic diversity was found for each locus studied. Most of the alleles were common to all the populations, indicating that the holm oak corresponds to a single genetic entity. Observation of breeding system characteristics showed that the holm oak is monoecious, wind-pollinated, and likely to possess genetic autoincompatibility. Furthermore, studies of flowering phenology in a single population showed that the period of flowering varied notably among trees. An average of 29% of the trees showed inter-annual variation in flowering time. The male, female or vegetative investment also varied widely among trees from one year to another. This results in a diversification of pollen source received by a given tree each year, and also from one year to another. 相似文献
99.
Dental agenesis in the Dariusleut Hutterite Brethren: comparisons to selected Caucasoid population surveys 总被引:1,自引:0,他引:1
We report the results of a study of the prevalence of nonsyndromic dental agenesis among a sample of 208 individuals (105 females, 103 males) between the ages of 15 and 29 years from a North American religious and genetic isolate, the Dariusleut Hutterites of Western Canada. Direct examination of dental casts, oral examination reports, dental treatment records, and a limited number of dental radiographs reveals congenital absence and/or obvious morphometric reduction of at least one tooth (excluding third molars) in 98 subjects (55 females, 43 males), yielding a prevalence estimate of approximately 47%. This estimate is nearly four times those reported for nonisolate Caucasoid populations of European descent and substantially higher than the elevated prevalences observed in several other isolated populations. Although the prevalence of dental agenesis in the Dariusleut is indeed high, neither the incidence of bilateral agenesis (exhibited at least once in 58% of affected dentitions), number of affected teeth per person (mean, 2.4), morphologic tooth classes affected, or combinations of tooth classes affected ostensibly distinguish them from other populations with similar geographic origins. We conclude that the dental agenesis observed in this North American genetic isolate does not represent a private polymorphism or rare developmental variant. Consequently, the results of further study in these Dariusleut Brethren will be directly relevant to critically testing as yet unresolved hypotheses for the mode of gene action and the relative contributions of hereditary and environmental factors to the reduction of tooth numbers in human dentitions. 相似文献
100.
G. G. Foster 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,63(4):295-305
Summary Genetic variability in the non-compound portion of the genomes of compound-chromosome (CC) strains intended for genetic control can be increased by the use of bridging strains which can be crossed to both CC and normal strains. Two bridging systems are described for chromosome-5 CC strains of Lucilia cuprina. The first system relies on the established viability and fertility of males trisomic for chromosome 5R. Males carrying the (5L.YL)23 half-translocation, a C(5R), and a normal chromosome 5 were crossed successfully to a CC strain and a normal strain. The second system uses a pair of reciprocal whole-arm 4;5 translocations to generate gametes disomic for 5R and nullosomic for 5L, which in combination with C(5L)-bearing gametes form viable near-euploid offspring with only small duplications and deficiencies. These offspring (C(5L); (4L.5R)357; (4R.5R)194; (4L.4R)) were crossed successfully with both CC and T(4;5)357/ + individuals. The latter were in turn crossed successfully with normal strains. The T(Y;5)23 system allows replacement of the non-CC genome with wild material more rapidly than the T(4;5)357/T(4;5)194 system, but unlike the latter does not allow replacement of the Y chromosome in the CC strain. The double translocation system is currently being used in L. cuprina. 相似文献