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101.
大蒜油诱发的小鼠腹腔中性粒细胞具有活跃的形态学特征,显示出强阳性的过氧化氢酶反应。与酵母聚糖(zymosan A)共同孵育,CeCl_3电镜细胞化学沉淀技术显示,中性粒细胞吞噬zymosan A后呈现强烈的H_2O_2生成反应。大蒜油诱发的大鼠腹腔中性粒细胞与~(51)Cr标记的S180肿瘤细胞共同孵育,它与肿瘤细胞的结合能力以及对肿瘤细胞的杀伤能力都高于糖原诱发的小鼠腹腔中性粒细胞。 相似文献
102.
《Saudi Journal of Biological Sciences》2022,29(4):2520-2525
Background and objectivesGarlic and its number of preparations are known to be effective for treatment of dyslipidemia, but the data about the specific active constituents of the garlic on the possible therapeutic value is scarce. Therefore, the aim of this research was to evaluate the role of garlic oil (GO) and its active element, diallyl disulphide (DADS) for obviating dyslipidemia in animal model.MethodsHigh fat diet (HFD) was given to animals to induce dyslipidemia. Animals of HFD groups were fed with atherogenic diet for 15 days prior to treatment. Animals in their respective groups received vehicle, GO (50 and 100 mg/kg), and DADS (4.47 and 8.94 mg/kg) for five consecutive days. Lipid profiles were estimated in serum, oxidant/antioxidant and liver profile were measured in liver tissue homogenate (LTH).ResultsAnimals fed on HFD developed significant increase in the serum levels of triglycerides (TG), total cholesterol (TC), lactate dehydrogenase (LDL), malondialdehyde (MDA), glutathione peroxidase (GSHPx), glutathione (GSH), and glutathione disulfide (GSSG) that reduced significantly in groups that received GO and DADS treatments. Additionally, significant elevation in serum high density lipoprotein (HDL) level was observed in animals that received GO and DADS. Moreover, hepatic markers such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine transferase (ALT), that were abnormally altered by high fat diet, were significantly restored to almost normal values with GO and DADS treatments. Also, antioxidants such as superoxide dismutase (SOD), catalase (CAT), ferric reducing antioxidant power (FRAP), and total thiol (SH) levels in LTH were increased significantly in GO and DADS treated groups. When compared to DADS, GO showed better therapeutic effectiveness in terms of antihyperlipidemic and antioxidant properties.ConclusionIn hyperlipidemic rats, garlic and its principal active component, diallyl disulphide, were effective in avoiding dyslipidemia and neutralizing reactive free radicals induced by a high fat diet. It's an intriguing observation that GO has a larger therapeutic influence than its active constituent, DADS. These findings suggest that other constituents, in addition to GO's DADS, are involved in the compound's synergistic antihyperlipidemic and antioxidant activities. 相似文献
103.
大蒜花叶病毒外壳蛋白基因cDNA的克隆和序列分析 总被引:1,自引:0,他引:1
我们从自然发病的大蒜中分离得到了大蒜花叶病毒。以其基因组RNA为模板合成了3'末端部分cDNA。从中选出一批插入片段在2.0kb以上的重组克隆,经Northern点杂交分析证实了所选克隆与基因组RNA同源。通过对若干个克隆的插入片段两端部分序列的测定,选出一个克隆pGM495,其插入片段的长度约为2.4kb,3′末端存有一个Poly(A)结构,它应包含了编码该病毒外壳蛋白全部序列。序列测定的结果表明,这个cDNA片段全长为2379bp,其中含有与酶切图谱分析结果相符的EeoRI、PstI及BamHI酶切位点。第一个终止密码子TAA与3′g末端相距264bp,我们根据碱基序列推定的氨基酸序列与其它已发表的Potyvirus的外壳蛋白氨基酸序列以及外壳蛋白翻译后加工的蛋白酶专一切点相比较后推测,编码该病毒外壳蛋白序列可能起始于3′末端上游的1170bp处,共编码302个氨基酸,其分子量为36kD,略大于SDS-PAGE所测定的33kD,非编码区域长264bp,富含AT,并有多个终止密码子的存在。趾3′末端32~27bp处有一个AATAA序列。 相似文献
104.
Transformation and regeneration of garlic (Allium sativum L.) by Agrobacterium-mediated gene transfer 总被引:5,自引:0,他引:5
By using highly regenerative calluses, we developed a stable transformation system in garlic (Allium sativum L.). The temperature and number of days of co-cultivation with Agrobacterium tumefaciens was shown to be an important factor in transient expression of the uid A gene. After a culture period of 5 months in selection medium containing hygromycin, 20 shoots were induced from ca. 1000
calluses, among which 15 plants expressed β-glucuronidase activity upon staining with X-Gluc. Shoots developed into transgenic garlic after 1 month. Integration of the uid A gene was confirmed by Southern blot analysis for genomic DNA of transgenic garlic plants.
Received: 25 October 1999 / Revision received: 16 February 2000 / Accepted: 22 February 2000 相似文献
105.
《Saudi Journal of Biological Sciences》2023,30(9):103749
Garlic (Allium sativum) is generally known to be of medicinal value, possessing potentials that include antimicrobial activity, but are often consumed in foods after subjection to cooking heat. The antimicrobial potential of heat stressed garlic may become decreased or lost when cooked, making its medicinal benefit unavailable to consumers. The potential of uncooked and cooked extracts from garlic imported to Jamaica, to inhibit the growth of eight microbes of clinical significance was investigated. Aqueous extracts of fresh garlic of 15 g/100 ml (fw), and dried and pulverized garlic cloves of 12.5 g/100 ml, 25 g/100 ml, 50 g/100 ml, and 100 g/100 ml (dw), were tested for inhibition of microbial growth. Extracts were tested uncooked, and cooked by boiling for 5, 10, and 15 min respectively. Of all the microbes studied, C. albicans incurred the largest zone of inhibition (57.7 ± 0.6 mm at the 100 g/100 ml of the dried extract, F(3, 8) = 51.778, p < 0.001, ω2 = 0.93). Cooking of garlic extracts resulted in statistically significant decreases in zones of inhibition of microbes, as evident in the linear regression and one-way ANOVA analyses, and/or complete loss of microbial inhibition. C. albicans was the most inhibited microbe, followed by E. coli, and Salmonella sp., respectively. The use of uncooked garlic may be the best route for obtaining the greatest antimicrobial potential of garlic against susceptible bacteria and fungi because cooking heat stress resulted in the decrease and complete loss of the antimicrobial potentials of the garlic. 相似文献
106.
Antonio Marques Susana Encarnação Sonia Pedro Maria Leonor Nunes 《World journal of microbiology & biotechnology》2008,24(10):2357-2360
Food can harbour harmful pathogenic microorganisms, such as Salmonella spp. that are responsible by serious human illnesses. The use of natural antimicrobial substances can be practical to maintain
the fresh image of food, to increase its shelf life, safety and marketability. We therefore evaluated the in vitro ability
of three natural antimicrobial substances (garlic, oregano and chitosan) to inhibit S. enterica under different thermal conditions. All substances exhibited strong antibacterial activities at low temperature. Oregano
had the strongest antibacterial properties, followed by chitosan and garlic. These results suggest that garlic, oregano and
chitosan can be practical to protect food and consumers from the risk of contamination by S. enterica, to extend food shelf-life, to reduce food waste and to use in hurdle technologies at low temperature. 相似文献
107.
108.
A restricted part of the undeveloped stem of the garlic clove, called the “stem disc”, which is just under the basement of
the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured
shoots consistently were differentiated from a single clove during 1 month of culture on phytohormone-free Linsmaier and Skoog
medium. In addition, more than 90% of the shoots formed bulblets in vitro during an additional 1 month of culture. Pretreatment
of the garlic bulbs at 4 °C for approximately 8 weeks before preparing the stem discs enhanced both shoot development and
bulblet formation. This novel method for culturing garlic was designated the stem-disc culture method. Shoot development in
this type of in vitro culture apparently is divided into four stages: expansion of tissue zones surrounded by the basal parts
of the immature foliage leaves, formation of dome-shaped structures, bud differentiation directly from each dome, and development
into shoots and bulblets. The dome-shaped structures appeared within 5 days of the onset of culture and had developed independently
into shoots approximately 1 cm high 3 weeks later. Histological observations showed that both the internal cell organization
and formation process of the dome-shaped structures were similar to those in the meristem. In addition, events leading to
the formation of these dome-shaped structures appeared to be initiated by vigorous cell division in the epidermis of concentric
tissue zones surrounded by the basements of immature foliage leaves. The results of several field trials showed that the stem-disc
culture method is useful for the production of garlic seed plants, including virus-free plantlets. Furthermore, it is a novel
field cultivation system for garlic in that the seedlings produced by in vitro-induced bulblets are used as seed instead of
the usual cloves.
Received: 25 November 1997 / Revision received: 4 February 1998 / Accepted: 21 February 1998 相似文献
109.
110.
Yan Wang Tingting Zou Minghui Xiang Chenzhong Jin Xuejiao Zhang Yong Chen 《Preparative biochemistry & biotechnology》2016,46(7):709-716
A soluble glycoprotein was purified to homogeneity from ripe garlic (Allium sativum) bulbs using ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl-52 cellulose anion-exchange chromatography. A native mass of 55.7?kDa estimated on gel permeation chromatography and a molecular weight of 13.2?kDa observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis supported that the glycoprotein is a homotetramer. β-Elimination reaction result suggested that the glycoprotein is an N-linked type. Fourier-transform infrared spectroscopy proved that it contains sugar. Gas chromatography–mass spectrometer analysis showed that its sugar component was galactose. The glycoprotein has 1,1-diphenyl-2-picrylhydrazil free radical scavenging activity and the peroxidation inhibition ability to polyunsaturated fatty acid. These results indicated that the glycoprotein has potential for food additives, functional foods, and even biotechnological and medical applications. 相似文献