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101.
Soniamol Joseph Kainoor K. Janardhanan Varughese George Sabulal Baby 《Phytochemistry letters》2011,4(3):386-388
A new epoxidic ganoderic acid, 8α,9α-epoxy-3,7,11,15,23-pentaoxo-5α-lanosta-26-oic acid (1), together with the known compounds 3β-hydroxy-7,11,15,23-tetraoxo-5α-lanosta-8-en-26-oic acid (2), ergosta-7,22-diene-3β-yl pentadecanoate (3), ergosta-7,22-diene-3β-ol (4), β-sitosterol (5), fatty acids (6–10), fatty acid ester (11) and octadecane (12) were isolated from the fruiting bodies of Ganoderma lucidum from south India. Their structures were determined by 1H, 13C, 13C DEPT, 1H–1H COSY, HMBC, HSQC, NOESY NMR, FT-IR, UV–vis and FABMS spectral analysis. Compounds (1–3) exhibited good antifungal activity against Candida albicans in disc diffusion assay (100 μg/disc). Steroid ester (3) showed moderate anti-inflammatory activity (59.7% inhibition, 100 mg/kg body weight) in carrageenan-induced paw edema. 相似文献
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103.
L. Rajendran R. Akila G. Karthikeyan T. Raguchander D. Saravanakumar R. Samiyappan 《Archives Of Phytopathology And Plant Protection》2013,46(6):690-702
Basal stem rot caused by Ganoderma lucidum is the most serious disease in coconut and arecanut gardens. Twenty-five Ganoderma isolates were collected from different parts of India and the pathogenicity of Ganoderma was proved on coconut seedlings. Mature sporophores developed within 10–13?weeks after inoculation of pathogen under in vivo. To detect the pathogen at early stage, DNA-based technology, polymerase chain reaction was used. In this, the primers Gan1 and Gan2 produced a product of 167?bp in size for all the Ganoderma isolates tested. Simultaneously, ITS 1 and ITS 4 primers amplified a fragment of 680?bp in the Ganoderma isolates. In addition, Ganoderma isolates showed polymorphism in the random amplified polymorphic DNA analysis. 相似文献
104.
M. Karthikeyan R. Bhaskaran K. Radhika S. Mathiyazhagan R. Sandosskumar R. Samiyappan 《Archives Of Phytopathology And Plant Protection》2013,46(7):507-513
Abstract An extracellular, hydrophilic, thermostable phytotoxin was purified to homogeneity from culture fluids of Ganoderma lucidum. The phytotoxin was purified by solvent extraction, gel filtration on Sephadex G-75. Toxicity was evaluated with detached leaf sheath and electrolyte leakage bioassays. Purified phytotoxin induced visible symptoms of the disease, when applied to coconut leaves, fronds and roots even at a low concentration. The toxin is a glycoprotein with carbohydrate as the major component. The importance of the carbohydrate moiety for toxic activity was indicated by inactivation of toxic compounds after periodate oxidation. The toxin caused lesions on a number of other monocots and dicots and proved to be non-host specific. 相似文献
105.
Yoshimitsu Yamazaki Hidekatsu Maeda 《Bioscience, biotechnology, and biochemistry》2013,77(6):1571-1581
A polymerizable NAD derivative, N6-[N-[N-(2-hydroxy-3-methacrylamidopropyl)carbamoylmethyl]carbamoylmethyl]-NAD, formate dehydrogenase, and malate dehydrogenase were entrapped all together in polyacrylamide gels. The entrapment was carried out by radical copolymerization, and consequently NAD was bound on the matrix which enclosed the enzymes. These gels had the function of producing l-malate from oxalacetate and formate. The l-malate production was also continuously done in a column reactor for 3 days. Another gel was similarly prepared with N6-[N-(6-methacrylamidohexyl)carbamoylmethyl]-NAD, horse liver alcohol dehydrogenase, and diaphorase. This gel was shown to catalyze the formation of resorufin from resazurin and ethanol. This gel was applicable to ethanol analysis using a fluorescence spectrophotometer to determine resorufin. The analyzer was usable for one week. 相似文献
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采用极性不同的6种溶剂(石油醚、乙酸乙酯、丙酮、乙醇、甲醇和水)、按索氏提取法逐级萃取破壁灵芝孢子粉,并同时运用气相色谱-质谱联用(GC/MS)和超高效液相串联四极杆飞行时间质谱(UPLC-QTOF/MS)技术对各萃取物进行化学成分分析与鉴定。结果表明:GC/MS共鉴定出101种化合物,其中酸类10种、酯类40种、醇类7种、酮类6种、酚类2种、烃类18种、甾类9种和杂原子化合物9种;UPLC-Q-TOF/MS共推断出40种化合物,其中倍半萜类1种、二萜类1种、三萜类9种、生物碱类4种、酰胺类7种、有机酸类9种以及其他化合物9种。两种测定方法间共有化合物仅1种,仅存在于5种有机溶剂(石油醚、乙酸乙酯、丙酮、乙醇和甲醇)萃取物之一的化合物共105种,2种或2种以上萃取物共有的化合物共31种,实验方法较好地实现了样品中化合物组分的充分分离,扩大了可检测化合物的范围。研究结果为灵芝孢子粉中化学成分的系统分析与鉴定、及灵芝孢子粉的化合物谱图库的完善提供了基础资料,为相关药理、药效分析及灵芝的药用模式真菌研究提供参考。 相似文献
109.
α-Glucosidase inhibitor has considerable potential as a diabetes mellitus type 2 drug because it prevents the digestion of carbohydrates. The search for the constituents reducing α-glucosidase activity led to the finding of active compounds in the fruiting body of Ganoderma lucidum. The CHCl3 extract of the fruiting body of G. lucidum was found to show inhibitory activity on α-glucosidase in vitro. The neutral fraction, with an IC50 of 88.7 μg/ml, had stronger inhibition than a positive control, acarbose, with an IC50 of 336.7 μg/ml (521.5 μM). The neutral fraction was subjected to silica gel column chromatography and repeated p-HPLC to provide an active compound, (3β,24E)-lanosta-7,9(11),24-trien-3,26-diol (ganoderol B). It was found to have high α-glucosidase inhibition, with an IC50 of 48.5 μg/ml (119.8 μM). 相似文献
110.
W.T.E. TingS.Y. Yuan S.D. WuB.V. Chang 《International biodeterioration & biodegradation》2011,65(1):238-242
Biodegradation of two polycyclic aromatic hydrocarbons (PAHs), phenanthrene and pyrene, by a white rot fungus, Ganoderma lucidum, in broth cultures was investigated. It was found that the biomass of the organism decreased with the increase of PAH concentration in the cultures. In the cultures with 2 to 50 mg l−1 PAHs, the degradation rate constants (k1) increased with the PAH concentration, whereas, at the level of 100 mg l−1, the degradation rate constants decreased. In the presence of 20 mg l−1 PAHs, the highest degradation rates of both PAHs occurred in cultures with an initial pH of 4.0 at 30 °C. The addition of CuSO4, citric acid, gallic acid, tartaric acid, veratryl alcohol, guaiacol, 2,2′-azino-bis-(3- ethylbenzothazoline-6-sulfonate) (ABTS) enhanced the degradation of both PAHs and laccase activities; whereas the supplement of oxalate, di-n-butyl phthalate (DBP), and nonylphenol (NP) decreased the degradation of both PAHs and inhibited laccase production. In conclusion, G. lucidum is a promising white rot fungus to degrade PAHs such as phenanthrene and pyrene in the environment. 相似文献