Alcian Blue Pyridine Variant–a Superior Alternative to Alcian Blue 8GX: Staining Performance and Stability

We compared the staining performance, dye content, solubility, and visual absorption maximum of two batches of alcian blue pyridine variant and of five batches of alcian blue 8GX (C.I. 74240). Whenever possible, we also compared results to those obtained with the same dye batches produced at an earlier date to provide information concerning dye stability. Both alcian blue pyridine variant batches were of high dye content, stable, of satisfactory solubility, and performed well in both the routine Mowry mucin stain and in the critical electrolyte concentration (CEC) stain. Of the five alcian blue 8GX samples, some were also of appropriate dye content, were sufficiently stable, and gave good staining in the two procedures. Two batches, however, were unstable, and three batches were unsatisfactory in staining performance and solubility in the CEC stain. Consequently alcian blue pyridine variant is a superior substitute for alcian blue 8GX.     

胃癌血管靶向肽GX2 对胃癌血管内皮细胞靶向性的鉴定

目的:证明胃癌血管靶向肽GX2能够与胃癌血管内皮细胞特异性结合,在体内对胃癌血管具有靶向性。方法:体外实验,合成GX2与FITC的复合物FITC-GX2,分离原代人脐静脉内皮细胞HUVEC,将HUVEC与人胃癌细胞系SGC7901共培养,建立共培养血管内皮细胞模型来模拟胃癌血管,利用免疫荧光的方法观察GX2与共培养内皮细胞Co-HUVEC的结合情况;体内实验,构建99mTc标记的GX2分子示踪探针,SPECT显像观察GX2的胃癌血管靶向性。结果:免疫荧光结果显示GX2能与Co-HUVEC特异性结合,而与人胃癌细胞SGC7901不结合;SPECT显像结果证明了GX2在荷瘤裸鼠体内能够浓集到肿瘤部位,具有良好的靶向性。结论:GX2能与胃癌血管内皮细胞特异性结合,且具有在体靶向到胃癌血管的能力;GX2具有胃癌诊断的潜在价值,并能应用于胃癌血管抑制治疗。     

新生血管特异性结合肽GX1二聚体抑制大鼠视网膜微血管内皮细胞血管生成的实验研究

目的:探讨新生血管特异性结合肽GX1二聚体对视网膜新生血管生成的影响。方法:化学合成GX1二聚体、GX1单体、对照肽二聚体,通过CCK-8实验、管状结构形成实验、迁移实验研究GX1二聚体对大鼠视网膜内皮细胞(RMEC)增殖、微管形成、迁移能力的影响,流式细胞学技术分析其对细胞周期分布和凋亡的影响。结果:CCK-8结果显示,与对照肽二聚体及阴性对照组相比,100-200μM GX1二聚体及单体均可抑制RMEC增殖(P<0.05),且随着GX1二聚体及单体浓度升高,抑制作用逐渐增强,呈剂量依赖性;各浓度GX1二聚体均较单体抑制作用增强,并有统计学差异(P<0.05)。管状结构形成实验、细胞损伤迁移实验结果显示与对照肽二聚体及PBS组相比,GX1二聚体及GX1单体均可明显抑制视网膜内皮细胞管状结构的形成及迁移,且二聚体抑制作用强于单体;对照肽二聚体仅有轻微的抑制视网膜内皮细胞管状结构形成的作用,对细胞迁移无明显抑制作用。流式细胞术分析显示与对照肽及阴性对照组相比,GX1二聚体及GX1单体均可诱导细胞凋亡(P<0.05),且GX1二聚体的诱导作用强于GX1单体(P<0.05),而对细胞周期分布则无明显影响。结论:GX1二聚体和GX1单体均可抑制视网膜新生血管内皮细胞增殖、微管形成、迁移能力及诱导凋亡,且GX1二聚体较GX1单体作用增强。GX1二聚体有望代替单体成为糖尿病视网膜病变新生血管靶向治疗小肽类药物。     

Novel Fixation of Plant Tissue, Staining through Paraffin with Alcian Blue and Hematoxylin, and Improved Slide Preparation

Onion (Allium cepa) root tips were fixed in a proprietary solution without aldehyde, toxic metals or acetic acid. Fixed specimens were embedded in paraffin, sectioned on a rotary microtome and mounted on detergent-washed slides without adhesive. Slides with ribbon segments affixed were immersed in 0.2% aqueous alcian blue 8GX in screw-capped Coplin jars in a water bath at 50 C for 1 hr. Excess alcian blue was rinsed off under cold running tap water and the slides were immersed in quick-mixed hematoxylin at room temperature for 15 min. Stained slides were deparaffinized, rinsed with isopropanol, air dried, and coverslips were affixed with resin. Thus, the traditional paraffin microtechnique has been modified at all steps from fixation to finishing slides with coverslips.