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31.
Summary The effect of supercooling and freezing on the photosynthetic capability of representatives of the permanent frost hardy giant rosette plants Dendrosenecio keniodendron, D. brassica and Lobelia telekii, of the tropical alpine regions was investigated with the non-invasive chlorophyll a fluorescence technique. While supercooling, normal chlorophyll a fluorescence kinetics exhibiting the sequence 0, I, (D), P, S, M, were recorded, however with some retardation of both, the fast and the slow characteristics as compared to those obtained at day-time temperature. As long as the leaves remained unfrozen, the rise of the variable fluorescence F
from the level 0 to P was inversely related to a drop of the temperature from about 0°C to-8°C. The increase of F
with lower temperature is understood to result from a decrease of the velocity of the quenching reactions while photoreduction of the primary electron acceptor appeared to be unimpeded. The second fluorescence maximum (M), usually interpreted to indicate the commencement of the biochemical reactions of photosynthesis was consistenly to be observed during supercooling. Fluoescence induction kinetics of frozen leaves showed only fast rise to presumably F
max which was not followed by a significant decay for as long as 4 min. The lack of substantial quenching indicates that in the freeze-dehydrated state neither reoxidation of the primary acceptor nor energetization of the thylakoid membrane was accomplished. This effect however was immediately and fully reserved upon thawing of the leaves when the usual fluorescence induction kinetics as well as normal rates of CO2-uptake were observed. Thus the permanent frost-hardy afroalpine plants do not exhibit any even short-term memory effect of the nocturnal frost on such a delicate process as is photosynthesis. 相似文献
32.
Five plant species were cultivated on a soil from the Neckar alluvial fan near Heidelberg (FRG) polluted by the emissions
of a cement plant. Thallium, cadmium and lead concentrations in seedlings and mature plants were determined by atomic absorption
analysis. AdditionallyBrassica napus L.napus was grown on soils containing 5 different concentrations of heavy metals, achieved by mixing two similar soils, from the
same area but with different metal concentrations.
Thallium and cadmium were shown to be taken up by roots whilst lead which was also absorbed, was deposited mainly on the plant
surface. However during cultivation in the winter months, a remarkable deposit of lead via the roots was found. Thallium in
the soil from a anthrorogen source was more available to plants than thallium of geological origin.
During the lifetime of a plant concentrations of thallium and cadmium were always highest in the seedling. The decrease in
metal concentration with maturity depended on the plant species and the element, but was not a function of the metal concentration
in the soil. 相似文献
33.
Endosymbiotic origin and codon bias of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize 总被引:19,自引:0,他引:19
Henner Brinkmann Pascal Martinez Françoise Quigley William Martin Rüdiger Cerff 《Journal of molecular evolution》1987,26(4):320-328
Summary The nuclei of plant cells harbor genes for two types of glyceraldehyde-3-phosphate dehydrogenases (GAPDH) displaying a sequence divergence corresponding to the prokaryote/eukaryote separation. This strongly supports the endosymbiotic theory of chloroplast evolution and in particular the gene transfer hypothesis suggesting that the gene for the chloroplast enzyme, initially located in the genome of the endosymbiotic chloroplast progenitor, was transferred during the course of evolution into the nuclear genome of the endosymbiotic host. Codon usage in the gene for chloroplast GAPDH of maize is radically different from that employed by present-day chloroplasts and from that of the cytosolic (glycolytic) enzyme from the same cell. This reveals the presence of subcellular selective pressures which appear to be involved in the optimization of gene expression in the economically important graminaceous monocots. 相似文献
34.
Influence of Hydrocortisone on Chick Embryo Retina Development 总被引:1,自引:1,他引:0
Renza Vento Giuseppina D'Ancona Michela Giuliano Gennaro Taibi Giovanni Tesoriere 《Journal of neurochemistry》1987,48(6):1693-1698
Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone. 相似文献
35.
36.
Bertil Löwkvist Hadar Emanuelsson Lo Persson Frank Sundler Anders Lundquist Dr. Olle Heby 《Cell and tissue research》1987,247(1):75-84
Summary The localization of ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis and thus in cell growth, was determined in the 4.5-day-old chick embryo, using two independent methods of analysis. ODC protein was identified by indirect immunofluorescence with a monospecific ODC antibody, and catalytically active ODC was identified by autoradiography with -(5-3H) difluoromethylornithine. Both methods revealed a basically similar distribution of ODC within the embryo. Among the organs, the brain exhibited the highest ODC levels. ODC levels were also high in spinal cord, mesonephric tubules and heart. Similar levels, but confined to limited areas, were found in liver tissue, head mesenchyme, and the oral and pharyngeal regions. Organs that exhibited high ODC levels are all engaged in rapid growth, as well as in extensive tissue remodeling and differentiation. 相似文献
37.
Methanogenesis by a Syntrophomonas wolfei/ Methanospirillum hungatei coculture was inhibited in presence of ethylene and the hydrogenation catalyst Pd-BaSO4. However, butyrate oxidation by S. wolfei continued and ethylene was reduced to ethane. Per mol of butyrate oxidized, 2.4 mol acetate was produced and 0.8 mol ethylene was reduced. Acetylene, propylene and butene were less effective as H2 acceptors than ethylene, and addition of bromoethanesulfonic acid was necessary to inhibit methanogenesis in the presence of the two longer-chain olefins. Other hydrogenation catalysts were less effective in the order Pd-charcoal < PE-asbestos < Pd-PEI beads < Pt-Al2O3, Pd-CaCO3. Optimal ethylene hydrogenation was achieved with still incubation in presence of 7.2 mg Pd-BaSO4 and 0.7 g sand per ml medium. The higher catabolic rate of S. wolfei in presence of the methanogen indicated that the biological H2 removal mechanism was more efficient than the catalytic olefin reduction.Abbreviations BES
bromoethane sulfonic acid
- VFA
volatile fatty acid 相似文献
38.
Deme formation in scale insects: a test with the pinyon needle scale and a review of other evidence 总被引:2,自引:0,他引:2
ABSTRACT. 1. Deme formation is the transformation of a generalist population into one which is adapted to its local conditions. This adaptation has been inferred from many things but should be inferred from higher survival or fecundity of scale insects on their natal tree compared to that of immigrant scales on the same tree.
2. Transfers of the scale insect Matsucoccus acalyptus Herbert within and between infested host trees ( Pinus monophylla (Torr. & Frem.) resulted in significant differences in scale survivorship among recipient trees. Survival on individual trees was correlated across years, indicating stable differences in tree susceptibility to scale.
3. A significant natal tree colonized tree interaction was observed for late stage scale survival in one experiment but the interaction was not caused by superior survivorship of scales transferred back to the natal tree. Hence, we found no evidence of deme formation in M.acalyptus.
4. Previous studies have concluded that deme formation occurs in the black pineleaf scale based on a significant natal tree by colonized tree interaction in scale survival. We question this conclusion because the experimental design employed did not include transfers back onto the natal tree. Other indirect evidence for deme formation in scale insects is critically discussed. 相似文献
2. Transfers of the scale insect Matsucoccus acalyptus Herbert within and between infested host trees ( Pinus monophylla (Torr. & Frem.) resulted in significant differences in scale survivorship among recipient trees. Survival on individual trees was correlated across years, indicating stable differences in tree susceptibility to scale.
3. A significant natal tree colonized tree interaction was observed for late stage scale survival in one experiment but the interaction was not caused by superior survivorship of scales transferred back to the natal tree. Hence, we found no evidence of deme formation in M.acalyptus.
4. Previous studies have concluded that deme formation occurs in the black pineleaf scale based on a significant natal tree by colonized tree interaction in scale survival. We question this conclusion because the experimental design employed did not include transfers back onto the natal tree. Other indirect evidence for deme formation in scale insects is critically discussed. 相似文献
39.
Expression vectors for cDNA of the κ and λ1 chains of a monoclonal antibody directed against creatine kinase were introduced into murine myeloma cells. κ and γ1 cDNA were either under the control of the SV40 early promoter or of the cognate promoters and enhancers of the light- and heavy-chain genes. Secretion of immuno-reactive κ and γ1 chains into the culture medium was demonstrated with the SV40 promoter as well as with the cognate promoters. Expression of y 1 cDNA with the SV40 early promoter was about twice as high as with the heavy-chain promoter and enhancer. Expression of κ cDNA under the control of the S V40 early promoter was about 17 times higher than with the light-chain promoter and enhancer. These expression levels were compared to those of a genomic immunoglobulin (Ig) κ determinant, including introns. Such an entire κ gene led to expression of the light chain at levels double those with the κ cDNA construction using the SV40 promoter and about 35 times as high when using κ cDNA and the cognate promoter and enhancer. This result might indicate that, besides the cognate promoter and enhancer elements, other intragenic elements are involved in the regulation of Ig expression. However, the SV40 early promoter seems to be able to compensate for the absence of these postulated regulatory elements probably located in the introns. 相似文献
40.
A field experiment was conducted at the Bangladesh Rice Research Institute, Joydebpur, Dhaka during the late wet season. Basal
application of P at both 5 and 10 kg ha−1 significantly increased total biomass production and nitrogen fixation byAzolla pinnata R. Brown (local strain). Addition of both 5 and 10 kg P ha−1 in equal splits at inoculation and at six day intervals thereafter during growth periods of 12, 24 and 36 days increased
biomass production and nitrogen fixation by Azolla over that attained with the basal application. Biomass and nitrogen fixation
using a split application of 5 kg P ha−1 exceeded that attained with basal application of 10 kg P ha−1 and split application of 10 kg P ha−1 resulted in 0.58, 11.2, and 18.3 t ha−1 more biomass, and 0.47, 18.9, and 18.3 more kg fixed N ha−1 at 12, 24 and 36 days, respectively, than the same amount applied as a basal application. Analyses indicated that the critical
level of dry weight P in Azolla for sustained growth was in the range of 0.15–0.17%. Compared with the control, where no P
was added, and additional 30 and 36 kg N ha−1 were fixed after 24 and 36 days, respectively, when P was provided at 10 kg ha−1 using a split application. A separate field study showed that flooded rice plants received P from incorporated Azolla with
about 28% of the P present in the supplied Azolla being incorporated into the rice plants. 相似文献