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891.
Quantifying photosynthetic capacity and its relationship to leaf nitrogen content for global-scale terrestrial biosphere models 总被引:3,自引:0,他引:3
JENS KATTGE WOLFGANG KNORR† THOMAS RADDATZ‡ CHRISTIAN WIRTH 《Global Change Biology》2009,15(4):976-991
Photosynthetic capacity and its relationship to leaf nitrogen content are two of the most sensitive parameters of terrestrial biosphere models (TBM) whose representation in global‐scale simulations has been severely hampered by a lack of systematic analyses using a sufficiently broad database. Here, we use data of qualitative traits, climate and soil to subdivide the terrestrial vegetation into functional types (PFT), and then assimilate observations of carboxylation capacity, Vmax (723 data points), and maximum photosynthesis rates, Amax (776 data points), into the C3 photosynthesis model proposed by Farquhar et al. to constrain the relationship of (Vmax normalised to 25 °C) to leaf nitrogen content per unit leaf area for each PFT. In a second step, the resulting functions are used to predict per PFT from easily measurable values of leaf nitrogen content in natural vegetation (1966 data points). Mean values of thus obtained are implemented into a TBM (BETHY within the coupled climate–vegetation model ECHAM5/JSBACH) and modelled gross primary production (GPP) is compared with independent observations on stand scale. Apart from providing parameter ranges per PFT constrained from much more comprehensive data, the results of this analysis enable several major improvements on previous parameterisations. (1) The range of mean between PFTs is dominated by differences of photosynthetic nitrogen use efficiency (NUE, defined as divided by leaf nitrogen content), while within each PFT, the scatter of values is dominated by the high variability of leaf nitrogen content. (2) We find a systematic depression of NUE on certain tropical soils that are known to be deficient in phosphorous. (3) of tropical trees derived by this study is substantially lower than earlier estimates currently used in TBMs, with an obvious effect on modelled GPP and surface temperature. (4) The root‐mean‐squared difference between modelled and observed GPP is substantially reduced. 相似文献
892.
Tabtoxin is a chlorosis-inducing toxin produced by the plant pathogenic bacterium Pseudomonas syringae pv. tabaci. Previous studies have indicated that tabtoxin inhibits glutamine synthetase (EC 6.3.1.2) in vitro. We report here that tabtoxin also inhibits glutamine synthetase in vivo. The main evidence was that assimilation of exogenous 15 NH3 into Asparagus sprengeri protein was rapidly inhibited in isolated cells exposed to tabtoxin. This was associated with an equivalent decline in glutamine synthetase activity in extracts of these cells and the accumulation of extracellular ammonia. Glutamine synthetase was also inhibited in leaves of Nicotiana tabacum L. cv. White Burley treated with tabtoxin and the affected tissue accumulated ammonia and became chlorotic. However, the development of symptoms and accumulation of ammonia was suppressed when the leaves were held in air containing 1% CO2 to reduce photorespiration. This indicates that the chlorotic symptom did not result from the inhibition of nitrogen assimilation but was a consequence of the interruption of the photorespiratory nitrogen cycle. 相似文献
893.
Formaldehyde is a well-known environmental toxic hazard. It is also a product of oxidative deamination of methylamine catalyzed by semicarbazide-sensitive amine oxidase (SSAO). Increased SSAO-mediated deamination has been implicated in some pathophysiological conditions, such as diabetic complications. The measurement of formaldehyde in the enzymatic reactions and in vivo production using conventional methods was not straightforward due to limitations of selectivity and sensitivity. A novel high-performance liquid chromatography (HPLC)/electrochemical procedure for the measurement of formaldehyde has been developed. The measurement is based on the formation of adducts between formaldehyde and dopamine. These adducts can be selectively purified and concentrated using a batch method of alumina absorption, separated by HPLC, and electrochemically quantified. The method is highly selective and substantially more sensitive, i.e., detection of picomole levels of formaldehyde, than the conventional methods. The procedure not only facilitates the assessment of SSAO activity in vitro but also is useful for assessing formaldehyde in tissues and biological fluids. 相似文献
894.
PERSPECTIVE: GENETIC ASSIMILATION AND A POSSIBLE EVOLUTIONARY PARADOX: CAN MACROEVOLUTION SOMETIMES BE SO FAST AS TO PASS US BY? 总被引:1,自引:0,他引:1
Abstract.— The idea of genetic assimilation, that environmentally induced phenotypes may become genetically fixed and no longer require the original environmental stimulus, has had varied success through time in evolutionary biology research. Proposed by Waddington in the 1940s, it became an area of active empirical research mostly thanks to the efforts of its inventor and his collaborators. It was then attacked as of minor importance during the "hardening" of the neo-Darwinian synthesis and was relegated to a secondary role for decades. Recently, several papers have appeared, mostly independently of each other, to explore the likelihood of genetic assimilation as a biological phenomenon and its potential importance to our understanding of evolution. In this article we briefly trace the history of the concept and then discuss theoretical models that have newly employed genetic assimilation in a variety of contexts. We propose a typical scenario of evolution of genetic assimilation via an intermediate stage of phenotypic plasticity and present potential examples of the same. We also discuss a conceptual map of current and future lines of research aimed at exploring the actual relevance of genetic assimilation for evolutionary biology. 相似文献
895.
Ben-Izhak Monselise E Parola AH Kost D 《Biochemical and biophysical research communications》2003,302(2):427-434
15N NMR analysis reveals alanine production in Duckweed plants exposed to low intensity sinusoidally varying magnetic fields (SVMF) at 60 and 100Hz, and fed by 15N-labeled ammonium chloride. Alanine does not accumulate in the absence of SVMF. Addition of vitamin C, a radical scavenger, reduced alanine production by 82%, indicating the roll of free radicals in the process. Alanine accumulation in plants and animals in response to exposure to a variety of stress conditions, including SVMF, is a general phenomenon. It is proposed that alanine is a universal first stress signal expressed by cells. 相似文献
896.
Brown KJ Downs CT 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2003,134(4):739-748
Digestive processes determine whether the particular diet of a bird is utilized efficiently and whether energetic demands are met. Assimilation efficiency is often used as an index of whether a diet is digested optimally. Studies on the digestive processing of generalist feeders are scarce. Cape White-eyes (Zosterops pallidus) have a diverse diet of fruit, nectar and insects. The nutrient contents of these three diets vary considerably and require quite different digestive processing. This study compared the digestive efficiencies of Cape White-eyes on these three diets by measuring transit times and assimilation efficiency. Cape White-eyes lost body mass significantly when fed fruit, while they maintained and gained body mass on nectar and mealworm diets, respectively. Assimilation efficiency varied significantly between the three diet types (nectar>mealworms>apples). When given a choice of diets, Cape White-eyes selected the diet, which was most efficiently digested and yielded the greatest energetic reward. Diet preference trials further showed that Cape White-eyes regulated daily energy intake. Assimilation efficiency depends on the accessibility of nutritional contents of a diet. Cape White-eyes did not maximize assimilation efficiency. Instead, they adjusted transit time to maximize the rate of energy gain per gram of food in order to maintain energy balance. 相似文献
897.
AIMS: To investigate the relationship between genomovar status and carbon source utilization, antibiotic susceptibility and growth ability on selective media of 142 clinical and environmental Burkholderia cepacia complex (Bcc) isolates belonging to all nine genomovars. METHODS AND RESULTS: Carbon source utilization and growth on selective media were tested by agar plate multipoint inoculation. Antimicrobial minimum inhibitory concentration (MIC) values were determined by agar dilution. Of all carbon sources, l-arabinose was most frequently utilized, supporting growth of 90% of all isolates. Burkholderia cepacia genomovar VI failed to utilize azelaic acid, penicillin G, phtalate, salicin and tryptamine. Overall, B. vietnamiensis and B. anthina were most susceptible and B. cepacia genomovar VI most resistant to antimicrobial agents. Burkholderia cepacia selective agar (BCSA) and the Mast B. cepacia medium supported growth of Bcc isolates most efficiently. CONCLUSIONS: This study demonstrates phenotypic heterogeneity within the Bcc. Some trends can be observed at the genomovar level, but only B. cepacia genomovar VI could be differentiated unambiguously on the basis of its inability to grow on PCAT. SIGNIFICANCE AND IMPACT OF THE STUDY: This work provides an update on some differential phenotypic characteristics of all nine Bcc genomovars. 相似文献
898.
C4 photosynthesis: discovery and resolution 总被引:1,自引:0,他引:1
Hatch MD 《Photosynthesis research》2002,73(1-3):251-256
This Minireview provides a brief account of the scene and interesting turn of events surrounding the discovery and resolution
of the mechanism of C4 photosynthesis, as well as the recognition of the process by the wider plant science community.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
899.
Pedro A. Sansberro Luis A. Mroginski Oscar A. Masciarelli Rubén Bottini 《Plant Growth Regulation》2002,38(3):231-236
The aim of this work was to analyse the response ofNH4
+ assimilation in leaves of tobacco plants(Nicotiana tabacum L. cv. Tennessee 86), to different Bapplications (B1, 5 M H3BO3; B2, 10M H3BO3; B3, 20 MH3BO3). The plants were grown under controlledenvironmental conditions and received a complete nutrient solution. In thisexperiment, we analysed the foliar concentrations of B andNH4
+, as well as the corresponding enzymaticactivities: GS,GOGAT, GDH, PEPC; the end products of this assimilation, aminoacids and proteins; and finally the concentration of non-structural sugars. Ourresults indicated that the different B treatments influenced the utilization ofNH4
+ by tobacco leaves. The B3 treatment registeredthe lowest NH4
+ concentration, and B1 the highest,due probably to the higher GS, GOGAT and GDH activities registered at B3.Conversely, a decline in the concentration of non-structural sugars wasrecordedat B3. In addition, the high assimilation rate caused a progressiveaccumulationof amino acids as well as proteins, and boosted biomass production in theleaves. 相似文献
900.