Individual age can be used to design more efficient and suitable management plans in both in situ and ex situ conservation programmes for targeted wildlife species. DNA methylation is a promising marker of epigenetic ageing that can accurately estimate age from small amounts of biological material, which can be collected in a minimally invasive manner. In this study, we sequenced five targeted genetic regions and used 8–23 selected CpG sites to build age estimation models using machine learning methods at only about $3–7 per sample. Blood samples of seven Felidae species were used, ranging from small to big, and domestic to endangered species: domestic cats (Felis catus, 139 samples), Tsushima leopard cats (Prionailurus bengalensis euptilurus, 84 samples) and five Panthera species (96 samples). The models achieved satisfactory accuracy, with the mean absolute error of the most accurate models recorded at 1.966, 1.348 and 1.552 years in domestic cats, Tsushima leopard cats and Panthera spp. respectively. We developed the models in domestic cats and Tsushima leopard cats, which were applicable to individuals regardless of health conditions; therefore, these models are applicable to samples collected from individuals with diverse characteristics, which is often the case in conservation. We also showed the possibility of developing universal age estimation models for the five Panthera spp. using only two of the five genetic regions. We do not recommend building a common age estimation model for all the target species using our markers, because of the degraded performance of models that included all species. 相似文献
This study provides information on habitat selection by the threatened Murray cod Maccullochella peelii peelii at two spatial scales in the Ovens and Murray Rivers in south-eastern Australia. Both adult (>450 mm total length, L T) and age 0 year (<150 mm L T) M. p. peelii selected macro and microhabitats based on structural variables. At the macrohabitat scale, adults selected channel habitats in the river, floodplain channels at high floods and within Lake Mulwala, whereas the floodplain proper was avoided. Adult and age 0 year fish selected similar microhabitats regardless of site or hydrologic conditions, and selection was primarily influenced by the presence of higher loadings of structural woody habitat, higher c.v. in depth, more overhanging vegetation, shallower comparative depths and lower water velocities, closer to the bank. Age 0 year M. p. peelii appeared to select shallower habitats with greater amounts of structural woody habitat, closer to the river bank than adult fish. 相似文献
The present study was conducted to examine further whether adult women who are in a state of high physical fitness possess
high physiological functions, and also to investigate whether those who exercise regularly are able to maintain a high quality
of various physiological functions. The subjects of this study were 249 healthy Japanese adult women (aged 20–70 years). Of
these subjects 30 had jogged or walked regularly for more than 3 years. The physiological ages (PA) and physical fitness ages
(FA) of the individuals were estimated from 17 physiological function tests and 5 physical fitness tests, respectively, by
principal components analyses. The results indicated that there was a significant correlation between PA and FA (r = 0.76, P < 0.01). To examine this relationship in more detail, the subjects were classified into three physical fitness groups (high,
normal and low) based on the deviation from the regression line of FA. Comparison of the mean PA among three physical fitness
groups revealed that the high physical fitness groups demonstrated a much lower PA (physiologically younger), while the low
physical fitness groups showed a relatively higher PA (physiologically older) in spite of their equivalent chronological ages.
From this series of studies, a new concept is proposed where different individuals have different peak physiological capacities,
but that these capacities change with age at similar rates. It is suggested that interventions such as exercise and a proper
diet for promoting health could increase peak functional capacity but have little effect on the rate of decline.
Accepted: 3 March 1998 相似文献
Previous studies have proposed terrestrial amphipods as potential bioindicators of forest condition. In order to investigate the response of the exotic terrestrial amphipod Talitroides topitotum (Crustacea: Amphipoda: Talitridae) to anthropogenic disturbances and its potential as a bioindicator, we compared its abundance among three forest reserves in southeastern Brazil, under different types and intensities of disturbance. We observed significantly higher abundances in disturbed sites compared to undisturbed sites in two of the reserves sampled, corroborating previous studies. In the third reserve, in which both forest disturbance and the abundance of amphipods were much lower than in the other two reserves, there was no significant difference between the sampling sites. We also speculate about the potential use of terrestrial amphipods as global indicators of forest disturbance. 相似文献
The EU Biodiversity Strategy stipulates in Target 2, Action 5 that the member states must map and assess the state of the ecosystems and their services and promote the integration into the reporting systems at the EU and national level by 2020. Therefore indicators for capturing and assessing ecosystem services (ES) are needed. In this paper we report for which ES class types currently ES indicators are being developed for Germany in the context of an ongoing research project. Additionally, we provide the indicator specifications, which are based on underlying framework concept. By way of the example of the provisioning service ‘raw wood production’ and the development of the main-indicator ‘annual wood accrual’ and six sub-indicators, we illustrate the concrete procedure, including discussion of results and target values. The indicators for the ES wood provision are not only suitable for an exemplary illustration of procedure, data selection and data basis in Germany. Furthermore, it shows that indicators for provisioning ES can eminently conflict with biodiversity and other ES. 相似文献