Tree ring responses to climate variability of xerophytic thickets from South Soalara,Madagascar

Xerophytic thickets occur along the southwestern part of Madagascar. Although providing a wide variety of resources and services to the local population, this particular vegetation is subjected to deforestation. This study focuses on linking dendroclimatology and dendroecology by examining the spatial and temporal variability of the ecological growth conditions. Information from tree rings was retrieved, on one hand, to identify the problem of the limiting effects of past climate on growth and, on the other hand, to show how local environment takes part in the growth pattern of South Soalara species, in the southwestern part of Madagascar. Methods and principles of dendrochronology were applied on nine species belonging to seven botanical families. A total of 42 stem discs from 3 to 5 trees per species were collected at 30 cm height. All discs exhibited visible tree rings, but anatomical distinctness varied between species. This study highlighted the annual formation of tree rings through successful crossdating techniques. Then, from the nine constructed chronologies, species were grouped into three clusters. Analysis between precipitation and radial growth showed that the response to climate occurred mainly in rainy season. Large-scale climatic drivers such as sea surface temperature (SST¹) of ENSO² regions revealed a teleconnection with tree growth in cluster 1. Mean radial increments were computed from the measured tree ring width and varied from 0.66 to 1.98 mm year⁻¹, showing that those species are slow-growing. All species were recorded as having a certain dendrochronological potential, which was ranked as useful for Rhizogum madagascariense and Terminalia gracilipes, poor for Gyrocarpus americanus and problematic for the other species. It is recommended to increase the number of wood samples and to provide more knowledge on the characteristics of the species in order to improve the quality of the chronology and the climatic signal on tree rings.     

Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah,Saudi Arabia

Little is known about the prevalence of HBV genotypes/sub-genotypes in Jeddah province, although the hepatitis B virus (HBV) was identified as the most predominant type of hepatitis in Saudi Arabia. To characterize HBV genotypes/sub-genotypes, serum samples from 15 patients with chronic HBV were collected and subjected to HBsAg gene amplification and sequence analysis. Phylogenetic analysis of the HBsAg gene sequences revealed that 11 (48%) isolates belonged to HBV/D while 4 (18%) were associated with HBV/C. Notably, a HBV/D sub-genotype phylogenetic tree identified that eight current isolates (72%) belonged to HBV/D1, whereas three isolates (28%) appeared to be more closely related to HBV/D5, although they formed a novel cluster supported by a branch with 99% bootstrap value. Isolates belonging to D1 were grouped in one branch and seemed to be more closely related to various strains isolated from different countries. For further determination of whether the three current isolates belonged to HBV/D5 or represented a novel sub-genotype, HBV/DA, whole HBV genome sequences would be required. In the present study, we verified that HBV/D1 is the most prevalent HBV sub-genotype in Jeddah, and identified novel variant mutations suggesting that an additional sub-genotype designated HBV/DA should be proposed. Overall, the results of the present HBsAg sequence analyses provide us with insights regarding the nucleotide differences between the present HBsAg/D isolates identified in the populace of Jeddah, Saudi Arabia and those previously isolated worldwide. Additional studies with large numbers of subjects in other areas might lead to the discovery of the specific HBV strain genotypes or even additional new sub-genotypes that are circulating in Saudi Arabia.     

Traveling behavior of a termite in tunnels with different curvatures and base surface roughness

This study focused on understanding the termite traveling behavior. Artificial tunnels with different curvatures and base surface roughness were constructed. Each tunnel was 50 mm in length, with widths of W (W = 2, 3, or 4 mm). The distance between the two ends of the tunnel was D (D = 20, 30, 40, or 50 mm). A higher value of D means a lower curvature. The roughness, R (R = 60, 120, 240 and ∞), was generated by uniformly sanding the substrate with a sanding machine. A higher R value means a finer base surface. The symbol ∞ represents a smooth surface. Time (τ) taken by a termite to pass through the tunnel was measured. τ was longer in tunnels with a smooth surface compared with the tunnels with a rough surface. When W = 2, there was no effect of the roughness on speed. This is because the narrow tunnel width diluted the effects of the smoothness of the surface. When W = 3, τ was statistically shorter for R = 240, than for R = 60 and 120. This suggests that an appropriate surface roughness could positively contribute to the traveling speed.     

Impact of priming on global soil carbon stocks

Fresh carbon input (above and belowground) contributes to soil carbon sequestration, but also accelerates decomposition of soil organic matter through biological priming mechanisms. Currently, poor understanding precludes the incorporation of these priming mechanisms into the global carbon models used for future projections. Here, we show that priming can be incorporated based on a simple equation calibrated from incubation and verified against independent litter manipulation experiments in the global land surface model, ORCHIDEE. When incorporated into ORCHIDEE, priming improved the model's representation of global soil carbon stocks and decreased soil carbon sequestration by 51% (12 ± 3 Pg C) during the period 1901–2010. Future projections with the same model across the range of CO₂ and climate changes defined by the IPCC‐RCP scenarios reveal that priming buffers the projected changes in soil carbon stocks — both the increases due to enhanced productivity and new input to the soil, and the decreases due to warming‐induced accelerated decomposition. Including priming in Earth system models leads to different projections of soil carbon changes, which are challenging to verify at large spatial scales.     

兰属春剑叶艺突变体叶片结构的研究

以兰属春剑(Cymbidium tortisepalum Fukuy.var.longibracteatum)叶色突变体‘隆昌素’及其亲本为材料,采用石蜡切片、扫描电镜及透射电镜对叶片的组织结构、扫描结构和超微结构进行了观察。结果显示,春剑叶色突变体叶片的表皮厚度和叶肉细胞面积均大于亲本,但导管数量少于亲本;叶片上、下表皮纹饰与亲本具有明显差异;气孔密度和面积均小于亲本,且气孔闭合度较大,占总气孔数的56%;突变体的叶绿体呈高密度的囊泡状结构,内部严重解体,体积较小的嗜锇滴聚集分布,无基粒片层,无淀粉粒。本研究表明经组培诱变的春剑叶色突变体的叶片结构与亲本存在明显差异。     

Lack of correlation between surface expression and currents in epileptogenic AB-calmodulin binding domain Kv7.2 potassium channel mutants

Heteromers of Kv7.2/Kv7.3 subunits constitute the main substrate of the neuronal M-current that limits neuronal hyper-excitability and firing frequency. Calmodulin (CaM) binding is essential for surface expression of Kv7 channels, and disruption of this interaction leads to diseases ranging from mild epilepsy to early onset encephalopathy. In this study, we addressed the impact of a charge neutralizing mutation located at the periphery of helix B (K526N). We found that, CaM binding and surface expression was impaired, although current amplitude was not altered. Currents were reduced at a faster rate after activation of a voltage-dependent phosphatase, suggesting that phosphatidylinositol-4,5-bisphosphate (PIP₂) binding was weaker. In contrast, a charge neutralizing mutation located at the periphery of helix A (R333Q) did not affect CaM binding, but impaired trafficking and led to a reduction in current amplitude. Taken together, these results suggest that disruption of CaM-dependent or CaM-independent trafficking of Kv7.2/Kv7.3 channels can lead to pathology regardless of the consequences on the macroscopic ionic flow through the channel.     

Fab is the most efficient format to express functional antibodies by yeast surface display

Multiple formats are available for engineering of monoclonal antibodies (mAbs) by yeast surface display, but they do not all lead to efficient expression of functional molecules. We therefore expressed four anti-tumor necrosis factor and two anti-IpaD mAbs as single-chain variable fragment (scFv), antigen-binding fragment (Fab) or single-chain Fabs and compared their expression levels and antigen-binding efficiency. Although the scFv and scFab formats are widely used in the literature, 2 of 6 antibodies were either not or weakly expressed. In contrast, all 6 antibodies expressed as Fab revealed strong binding and high affinity, comparable to that of the soluble form. We also demonstrated that the variations in expression did not affect Fab functionality and were due to variations in light chain display and not to misfolded dimers. Our results suggest that Fab is the most versatile format for the engineering of mAbs.     

Natural products as modulators of the nuclear receptors and metabolic sensors LXR,FXR and RXR

Nuclear receptors (NRs) represent attractive targets for the treatment of metabolic syndrome-related diseases. In addition, natural products are an interesting pool of potential ligands since they have been refined under evolutionary pressure to interact with proteins or other biological targets.This review aims to briefly summarize current basic knowledge regarding the liver X (LXR) and farnesoid X receptors (FXR) that form permissive heterodimers with retinoid X receptors (RXR). Natural product-based ligands for these receptors are summarized and the potential of LXR, FXR and RXR as targets in precision medicine is discussed.     

Reduction of bacterial adhesion on titanium-doped diamond-like carbon coatings

A range of titanium doped diamond-like carbon (Ti-DLC) coatings with different Ti contents were prepared on stainless steel substrates using a plasma-enhanced chemical vapour deposition technique. It was found that both the electron donor surface energy and the surface roughness of the Ti-DLC coatings increased with increasing Ti contents in the coatings. Bacterial adhesion to the coatings was evaluated against Escherichia coli WT F1693 and Pseudomonas aeruginosa ATCC 33347. The experimental data showed that bacterial adhesion decreased with the increases of the Ti content, the electron donor surface energy and surface roughness of the coatings, while the bacterial removal percentage increased with the increases of these parameters. The Ti-DLC coatings reduced bacterial attachment by up to 75% and increased bacterial detachment from 15 to 45%, compared with stainless steel control.     

Fungal Communities on Rock Surfaces in Demänovská Ice Cave and Demänovská Cave of Liberty (Slovakia)

This paper is the first geomycological report regarding the fungal communities on rock surfaces in the Demänovská Ice Cave and the Demänovská Cave of Liberty, Slovakia. The samples were collected in June 2014 from five locations from inside both the caves by using sterile swabs wetted with physiological saline (0.85% NaCl). The density of epilithic fungi isolated from the Demänovská Ice Cave ranged from 238.7 to 575.1 CFU (colony-forming units) per m² of the rock surface, and from the Demänovská Cave of Liberty ranged from 88.6 to 347 CFU. Seventeen different free-living culturable fungi (15 filamentous fungi, one yeast, and one yeast-like fungus) were isolated from the rock surfaces of both caves. Generally, Cladosporium cladosporioides and Aspergillus flavus were the most frequently cultured species from the Demänovská Ice Cave and the Demänovská Cave of Liberty, respectively. Free-living fungi found on the rock surfaces of both caves can lead to their slow biodegradation.