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131.
In hardwood subtropical forests of southern Florida, nonnative vines have been hypothesized to be detrimental, as many species form dense “vine blankets” that shroud the forest. To investigate the effects of nonnative vines in post‐hurricane regeneration, we set up four large (two pairs of 30 X 60 m) study areas in each of three study sites. One of each pair was unmanaged and the other was managed by removal of nonnative plants, predominantly vines. Within these areas, we sampled vegetation in 5 X 5 m plots for stems 2 cm DBH (diameter at breast height) or greater and in 2 X 0.5 m plots for stems of all sizes. For five years, at annual censuses, we tagged and measured stems of vines, trees, shrubs and herbs in these plots. For each 5 X 5 m plot, we estimated percent coverage by individual vine species, using native and nonnative vines as classes. We investigated the hypotheses that: (1) plot coverage, occurrence and recruitment of nonnative vines were greater than that of native vines in unmanaged plots; (2) the management program was effective at reducing cover by nonnative vines; and (3) reduction of cover by nonnative vines improved recruitment of seedlings and saplings of native trees, shrubs, and herbs. In unmanaged plots, nonnative vines recruited more seedlings and had a significantly higher plot‐cover index, but not a higher frequency of occurrence. Management significantly reduced cover by nonnative vines and had a significant overall positive effect on recruitment of seedlings and saplings of native trees, shrubs and herbs. Management also affected the seedling community (which included vines, trees, shrubs, and herbs) in some unanticipated ways, favoring early successional species for a longer period of time. The vine species with the greatest potential to “strangle” gaps were those that rapidly formed dense cover, had shade tolerant seedling recruitment, and were animal‐dispersed. This suite of traits was more common in the nonnative vines than in the native vines. Our results suggest that some vines may alter the spatiotemporal pattern of recruitment sites in a forest ecosystem following a natural disturbance by creating many very shady spots very quickly. 相似文献
132.
Characterization of kidney epithelial cells from the Florida manatee, Trichechus manatus latirostris
Summary The West-Indian manatee,Trichechus manatus latirostris, is a herbivorous marine mammal found in the coastal waters of Florida. Because of their endangered status, animal experimentation
is not allowed. Therefore, a cell line was developed and characterized from tissue collected during necropsies of the manatees.
A primary cell culture was established by isolating single cells from kidney tissue using both enzymatic and mechanical techniques.
Primary manatee kidney (MK) cells were subcultured for characterization. These cells were morphologically similar to the cell
lines of epithelial origin. An immunocytochemistry assay was used to localize the cytokeratin filaments common to cells of
epithelial origin. At second passage, epithelial-like cells had an average population-doubling time of 48 h, had an optimum
seeding density of 5×103 cells/cm2, and readily attached to plastic culture plates with a high level of seeding efficiency. Although the epithelial-like cells
had a rapid growth rate during the first three passages, the cloning potential was low. These cells did not form colonies
in agar medium, were serum dependent, had a limited life span of approximately nine passages, and possessed cell-contact inhibition.
These data suggest that the cells were finite (noncontinuous growth), did not possess transformed properties, and were of
epithelial origin. These cells are now referred to as MK epithelial cells. 相似文献
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Abstract. We studied the effect of burning frequency on the density and species richness of understory flowering stems in a Florida sandhill. Flowering stems were censused weekly for 54 weeks in six sites that had been burned one to six times in the previous 16 years. We concurrently measured overstory characteristics such as species composition, density and basal area. We used maximum likelihood and Akaike's Information Criterion to compare linear, quadratic, saturating, and null models of community response to repeating burning. We did not find a relationship between species richness, diversity or flowering stem density and fire frequency. Tree density was related to fire frequency and may represent an indirect pathway for fire effects on understory characteristics. While we found no support for the Intermediate Disturbance Hypothesis, an analysis of our experimental design indicated that we had low statistical power. We develop the hypothesis that a saturating model of response to fire best describes understory species richness in our system. We test this hypothesis using the most extensive published fire data set we are aware of and find support for a saturating model. 相似文献
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BackgroundCancer stage at diagnosis is a critical prognostic factor regarding a patient’s health outcomes. It has yet to be shown whether insurance status across different race has any implications on the stage of disease at the time of diagnosis. This study aimed to investigate whether insurance status was a modifier of the association between race and stage of previously undetected prostate cancer at the time of diagnosis in Florida between 1995 and 2013.MethodsSecondary data analysis of a cross-sectional survey using information from the Florida Cancer Data System (n = 224,819). Study participants included black and white males diagnosed with prostate cancer in Florida between 1995 and 2013. The main outcome variable was stage of prostate cancer at diagnosis. The main independent variable was race (black vs white). Adjusted logistic regression models were used to explore the association between race, insurance status and stage at diagnosis. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated.ResultsBlack males were more likely to be diagnosed with late stage prostate cancer (OR 1.31; 95% CI 1.27–1.35). Being uninsured (OR 2.28; 95% CI 2.13–2.45) or having Medicaid (OR 1.84; 95% CI 1.70–1.98) was associated with a diagnosis of late stage cancer. Stratified analysis for health insurance revealed that blacks had an increased risk for late stage cancer if uninsured (OR 1.29; 95% CI 1.07–1.55) and if having Medicare (OR 1.39; 95% CI 1.31–1.48).Conclusion: Insurance status may modify the effect of race on late stage prostate cancer in black patients. 相似文献
139.
Mottled ducks (Anas fulvigula) are endemic to the Gulf Coast of North America, and their range stretches from Alabama to the Laguna Madre of Mexico, with a distinct population in peninsular Florida and an introduced population in South Carolina. As one of the few non-migratory ducks in North America, mottled ducks depend on a variety of locally available habitat throughout the annual cycle, and threats to these landscapes may affect mottled ducks more acutely than migratory species. Annual population monitoring has revealed declines in mottled duck populations in Texas and Louisiana since 2008, and the genetic integrity of the Florida population has been muddled by the presence of large numbers of feral mallards (Anas platyrhynchos) resulting in hybridization. Similar to other closely related dabbling ducks, mottled duck populations are influenced by recruitment and breeding season survival, so changes in these factors may contribute to population decline. Accordingly, researchers have attempted to address various aspects of mottled duck breeding season ecology and population dynamics since the 1950s. We conducted a literature review on this topic by searching a combination of key terms using Google Scholar, including mottled duck, nesting ecology, habitat use, breeding incidence, nest success, brood, and breeding season survival, and followed citation trees to eventually aggregate information from nearly 50 publications on mottled duck breeding ecology. Our review concluded that mottled ducks use brackish and intermediate coastal marsh, including managed impoundments, and agricultural land during the breeding season. Their nests can be found in pastures, levees, dry cordgrass marsh, cutgrass marsh, spoil banks, and small islands. Nesting propensity and nest success estimates are often lower than other waterfowl species that are characterized by stable or increasing populations. Broods use wetlands composed of a mix of open water with submerged and emergent vegetation. Breeding season survival is higher for the Florida population than the western Gulf Coast population, but adult survival in both geographies is comparable to (or higher than) that of other dabbling duck species. Breeding habitat use, breeding season survival, and nest-site selection and success have been studied extensively in mottled ducks, whereas information on nesting propensity, renesting intensity, and post-hatch ecology is lacking. © 2021 The Wildlife Society. 相似文献
140.
There is a great importance for undergraduate biology students to study organisms in their natural context. Safety concerns surrounding the global COVID‐19 pandemic prevented Marine Invertebrate Zoology students at the University of Tampa from participating in traditional faculty‐led field trips during the Fall of 2020. Instead, students were assigned to conduct a diversity‐focused field trip on their own and report their findings. Here we describe considerations and methods for creating a safe and valuable self‐guided field trip assignment for upper‐level invertebrate zoology students. These methods are adaptable for a variety of different habitat types and can be conducted with little to no special equipment or training. Students were successful in completing this assignment and found it highly enriching. 相似文献