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71.
Ca-pumps in smooth muscle: one in plasma membrane and another in endoplasmic reticulum 总被引:4,自引:0,他引:4
A K Grover 《Cell calcium》1985,6(3):227-236
For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed here to hopefully resolve this issue. The inescapable conclusion is that there are two modes of nonmitochondrial ATP-dependent Ca-transport. The first one, unaffected by oxalate, is localized in the plasma membranes and the second, potentiated by oxalate, is localized in the endoplasmic reticulum. Clear experiments to delineate the roles of the two pumps in the excitation-contraction cycle of the smooth muscle remain to be conducted. 相似文献
72.
Most previously published electromyographic (EMG) studies have indicated that the temporalis muscles in humans become almost
electrically quiet during incisai biting. These data have led various workers to conclude that these muscles may contribute
little to the incisai bite force. The feeding behavior and comparative anatomy of the incisors and temporalis muscles of certain
catarrhine primates, however, suggest that the temporalis muscle is an important and powerful contributor to the bite force
during incision. One purpose of this study is to analyze the EMG activity of the masseter and temporalis muscles in both humans
and macaques with the intention of focusing on the conflict between published EMG data on humans and inferences of muscle
function based on the comparative anatomy and behavior of catarrhine primates. The EMG data collected from humans in the present
study indicate that, in five of seven subjects, the masseter,anterior temporalis, and posterior temporalis muscles are very active during apple incision (i.e., relative to EMG activity
levels during apple and almond mastication). In the other two human subjects the EMG levels of these muscles are lower during
incision than during mastication, but in no instance are these muscles ever close to becoming electrically quiet. The EMG
data on macaques indicate that, in all six subjects, the masseter, anterior temporalis, and posterior temporalis muscles are
very active during incision. These data are in general agreement with inferences on muscle function that have been drawn from
the comparative anatomy and behavior of primates, but they do not agree with previous experimental data. The reason for this
disagreement is probably due to differences in the experimental procedure. In previous studies subjects simply bit isometrically
on their incisors and the resulting EMG pattern was compared to the pattern associated with powerful clenching in centric
occlusion. In the present study the subjects incised into actual food objects, and the resulting EMG pattern was compared
to the pattern associated with mastication of various foods. It is not surprising that these two procedures result in markedly
different EMG patterns, which in turn result in markedly different interpretations of jaw-muscle function. In an attempt to
explain the evolution of the postorbital septum in anthropoids, it has been suggested that the anterior temporalis is more
active than the masseter during incision (Cachel, 1979). The human and macaque EMG data do not support this hypothesis; during
incision, the two muscles show no consistent differences in humans and the masseter appears to be in fact more active than
the anterior temporalis in macaques. 相似文献
73.
The influence of sex steroid and pregnancy on the tissue concentration, uterine motor effect and receptor binding of VIP has been studied in the female genital tract of pregnant rabbits and oophorectomized rabbits during progesterone and/or oestrogen substitution. The concentration of immunoreactive VIP was high in the vagina and cervix, and lower in the uterine body of both pregnant and non-pregnant rabbits. A significant decrease in the VIP concentration (pmol/g wet weight) of the uterine body was observed toward term of pregnancy. The total uterine content of VIP, however, seems unchanged. Treatment of oophorectomized rabbits with ovarian steroids had no effect on the VIP concentration. The sensitivity for and potency of VIP on the relaxation of uterine muscle was significantly higher in oophorectomized rabbits treated with a combination of progesterone and oestrogen than in control rabbits. No difference was observed between non-pregnant and pregnant rabbits. The degradation and binding affinity for 125I-labelled VIP was highest in oophorectomized rabbits substituted with both oestrogen and progesterone. In the pregnant rabbits, the amount of receptors was decreased near term. In conclusion, sex steroids are able to influence the motor effect of VIP at the receptor level, but have no effect on the VIP concentration in the female genital tract. 相似文献
74.
Effect of calcitonin gene-related peptide on the neuroeffector mechanism of sympathetic nerve terminals in rat vas deferens 总被引:2,自引:0,他引:2
In order to evaluate the mode of action of calcitonin gene-related peptide (CGRP) on the neuroeffector mechanism of peripheral sympathetic nerve fibers, the effects of CGRP were tested on the electrical stimulated and the non-stimulated preparations of the isolated rat vas deferens. The contractile responses, which were mediated predominantly by activation of postganglionic noradrenergic nerve fibers, were dose-dependently inhibited by CGRP in concentrations ranging from 0.1 to 10 nM. The inhibitory response produced by CGRP in high concentrations (greater than 2 nM) usually returned to the control level at 20-30 min and were rarely tachyphylactic. The inhibitory action of CGRP was not modified by pretreatment with 10(-7) M propranolol or 10(-7) M atropine. Contractions produced by exogenous norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in unstimulated preparations were not affected by pretreatment with CGRP in a low concentration (less than 2 nM). On the other hand, the contractions were slightly reduced 1 min after pretreatment with CGRP in high concentrations (greater than 5 nM), which recovered in 15 min after constant flow washout. High concentrations of CGRP also caused a concentration-dependent relaxation on the precontracted preparations produced by high potassium (60 mM K+) solution. These results suggest that CGRP in high concentrations (greater than 5 nM) may have a non-specific inhibitory action on the postsynaptic plasma membrane of the smooth muscle cell and a postulated CGRP receptor exists presynaptically in the rat vas deferens and that CGRP may inhibit the release of NE during adrenergic nerve stimulation. 相似文献
75.
Dr. Berend van der Lei Charles R. H. Wildevuur Paul Nieuwenhuis Engbert H. Blaauw Freark Dijk Caesar E. Hulstaert Izaäk Molenaar 《Cell and tissue research》1985,242(3):569-578
Summary The ultrastructure of a new type of vascular graft, prepared from a mixture of polyurethane (95 weight %) and poly-L-lactic acid (5 weight %), was examined six weeks after implantation into the abdominal aorta of rats. These microporous, compliant, biodegradable, vascular grafts function as temporary scaffolds for the regeneration of the arterial wall.Smooth muscle cells, covering the grafts, regenerated a neo-media underneath an almost completely regenerated endothelial layer (neo-intima). These smooth muscle cells varied in morphology from normal smooth muscle cells to myofibroblasts. They were surrounded by elastic laminae and collagen fibers.Macrophages, epithelioid cells, multinucleated giant cells, fibroblasts and capillaries were present in the disintegrating graft lattices. The epithelioid cells and multinucleated giant cells engulfed polymer particles of the disintegrating grafts.The regeneration of the endothelial and smooth muscle cells is similar to the natural response of arterial tissue upon injury. The presence of macrophages, epithelioid cells, multinucleated giant cells, fibroblasts and capillaries in the graft lattices resembles the natural response of tissue against foreign body implants. Both of these responses result in the formation of a neo-artery that possesses sufficient strength, compliance and thromboresistance to function as a small caliber arterial substitute.Supported by Grant nr. 82.042 from the Dutch Heart Foundation 相似文献
76.
A method is proposed to analyse the dispersion profiles of species in classes of environmental variables, based on the decomposition of the expected frequencies in contingency tables with many interacting species. The method has been applied to data of dominant or very frequent graminoid species in grasslands of the Natisone Valley (Friuli, Italy). It allowed to make predictions by removing the random component of variation.Nomenclature follows: P. Zangheri (1976). Flora Italica, CEDAM, Padua.The authors were recipients of an Italian CNR grant (E. Feoli) and a Canadian NSERC grant (L. Orlóci) during tenure of this project. The results are in partial fulfillment of a commitment to the Centro Regionale per la Sperimentazione Agraria per il Friuli-Venezia Giulia. 相似文献
77.
Two phosphoglucose isomerases (PGI) with different electrophoretic mobilities have been found in all groups of teleostean fishes studied, with the exception of the Clupeomorpha. PGI proved to be a good taxonomic criterion to differentiate members of the Nemipteridae, Sciaenidae, Platycephalidae and Stromateidae from the other teleost families. 相似文献
78.
G. H. Okker-Reitsma I. J. Dziadkowiec C. G. Groot 《In vitro cellular & developmental biology. Plant》1985,21(1):22-25
Summary A short method is described for obtaining a large number of pure vascular smooth muscle cells in culture. The smooth muscle
cells were isolated from human umbilical cord arteries digested twice by an enzyme mixture of collagenase, trypsin, elastase,
and DNAase with addition of α-tosyl-lysyl chloromethane. Primary cell culture and first subculture were not contaminated by
endothelial cells, no Factor VIII being produced. The cultures consisted of smooth muscle cells as appeared from phase contrast
and electron microscopy.
Part of this study was supported by a scholarship from the Dutch Ministry of Education and Science and by the Leyden University
Foundation. 相似文献
79.
Evidence for the Neurotrophic Regulation of Collagen-Tailed Acetylcholinesterase in Immature Skeletal Muscle by β-Endorphin 总被引:1,自引:1,他引:0
Laurence W. Haynes Margaret E. Smith Derek G. Smyth† 《Journal of neurochemistry》1984,42(6):1542-1551
Abstract: Acetylcholinesterase (AChE) was extracted in a high-saline medium from gastrocnemius muscles of rat embryos and young rats aged 14 days'gestation to 40 days post partum. The molecular forms of the enzyme were separated by low-salt precipitation, followed by velocity sedimentation. During gestation, all molecular forms increased in activity, particularly the 16 S (A12 ) form. During the first 2 weeks of life, there was a large increase in the activity of soluble AChE (G forms), whilst the activity of insoluble AChE (A forms) was reduced. Denervation of the muscle reversed the change in the relative proportions of the molecular forms. The embryonic pattern of activities of AChE forms persisted in cultures of myotubes obtained at 20 days'gestation and maintained in the absence of spinal cord. When myotubes were maintained in medium previously conditioned by developing spinal cord explants, 16 S AChE declined while the soluble (4 and 6 S) forms increased in activity in a manner resembling that seen in early postnatal muscles in vivo . β-Endorphin (β-EP) immunoreactivity was detected in the spinal cord-conditioned medium and was identified by HPLC and ion-exchange chromatography as β-EP-(l–31) plus its shortened and N -acetylated forms. Cultivation of myotubes in the presence of synthetic camel β-EP resulted in a reversible change in the pattern of AChE forms which was similar to that seen with spinal cord-conditioned medium. These studies provide evidence for the neuroregulation of AChE A and G forms in immature skeletal muscle. A major candidate for this role is β-EP, produced and released by developing spinal cord. 相似文献
80.
In 12 h fasted rats, rates of muscle protein synthesis were stimulated by refeeding for 1 h and by intragastric or intravenous infusion of an amino acid plus glucose mixture for 1 hr, but not by intravenous infusion of amino acids alone for 1 h. Intravenous injection of anti-insulin serum suppressed the response to feeding and to intragastric infusion, but not to intravenous infusion. It is concluded that the response of muscle protein synthesis to food intake is mediated by both insulin and amino acids acting in concert. 相似文献