HFRS患者特异性IgA,IgE抗体及其免疫复合物测定

为进一步研究ＨＦＲＳ免疫损伤机制,用ＥＬＩＳＡ法同步测定了１０８例不同临床型、不同病日、病期ＨＦＲＳ患者血清中特异性ＩｇＡ、ＩｇＥ抗体以及ＨＦＲＳ病毒特异性ＩｇＡ、ＩｇＥ型ＣＩＣ的水平及检出率。发现ＨＦＲＳＩｇＡ型抗体在轻型病例高于中、重型病例;ＨＦＲＳＩｇＥ型抗体及ＩｇＥ型ＣＩＣ在重型病例高于中、轻型病例。上述差异在病程早期（发热、休克少尿期,或是３～８病日）尤为突出。ＩｇＡ型ＣＩＣ则未见到上述差异。     

Hemorrhagic fever with renal syndrome complicated by orchitis

Hemorrhagic fever with renal syndrome (HFRS) is a disease caused by viruses of the family Bunyaviridae,genus Hantavirus.HFRS from Dobrava virus (DOBV) is a seldom reported disease in Albania.Clinically HFRS is manifested as mild,moderate,or severe.Therefore,the number of cases of Hantavirus'infection may be underestimated,and should be included in the differential diagnosis of many acute infections,hematologic diseases,acute abdominal diseases and renal diseases complicated by acute renal failure.We report here an atypical presentation of HFRS from Dobrava virus complicated by orchitis with a positive outcome.     

A method for detecting rash and fever illness‐associated viruses using multiplex reverse transcription polymerase chain reaction

In this study, a new multiplex RT‐PCR method for detecting various viral genes in patients with rash and fever illnesses (RFIs ) was constructed. New primer sets were designed for detection of herpes simplex viruses 1 and 2 (HSV1 and 2), and Epstein–Barr virus (EBV). The newly designed and previously reported primer sets were used to detect 13 types of RFI‐associated viruses by multiplex RT‐PCR assay systems. Moreover, to eliminate non‐specific PCR products, a double‐stranded specific DNase was used to digest double‐stranded DNA derived from the templates in clinical specimens. RFI‐associated viruses were detected in 77.0% of the patients (97/126 cases) by the presented method, multiple viruses being identified in 27.8% of the described cases (35/126 cases). Detected viruses and clinical diagnoses were compatible in 32.5% of the patients (41/126 cases). Sensitivity limits for these viruses were estimated to be 10¹–10³ copies/assay. Furthermore, non‐specific PCR products were eliminated by a double‐stranded specific DNase with no influence on sensitivity. These results suggest that this method can detect various RFI‐associated viruses in clinical specimens with high sensitivity and specificity.

     

感染流行性出血热病毒的正常人骨髓细胞膜脂和酶生物活性的研究

采用疏水性荧光探针ＤＰＨ（１,６,－苯基－１,３;５－已三烯）标记感染了流行性出血热病毒的正常人骨髓细胞的细胞膜,根据荧光偏振值的变化推导出膜脂流动性,同时通过细胞组化染色定量方法,测定细胞内酸性磷酸酶（ＡＣＰ）和琥珀酸脱氢酶（ＳＤＨ）的活性。动态观察１、６、２４、７２ｈ培养后的淋巴细胞、单核细胞、中性粒细胞,发现感染后细胞膜脂的流动性明显降低,并随培养时间的延长而明显,和正常对照组比较有显著性差异（Ｐ＜０．０５～０．０１）。通过组化染色和显微分光光度计单细胞扫描定量认为：感染细胞在培养开始的７２ｈ内ＡＣＰ、ＳＤＨ升高,１２０ｈ后明显下降,和正常相比亦有显著差异（Ｐ＜０．０５）,这与病毒侵犯和细胞机能的受损程度有关。     

流行性出血热病毒对乳鼠致病特点的观察

本文观察了两株从病人体内新分离的流行性出血热(EHF)病毒114株和435株对乳鼠的致病特点,并在感染的乳鼠脑内找到了EHF病毒。免疫荧光检查发现,病毒抗原广泛存在于感染鼠的脑、肺、肝、肾等脏器。脑内和腹腔两条感染途径的比较发现,病毒抗原在上述脏器中的分布基本一致,但前者脑内的病毒感染滴度较后者高一个对数单位。对病毒的动态观察发现,EHF病毒首先在乳鼠腹腔感染6小时后的腹腔巨噬细胞(Mφ)中分离到、并持续阳性;病毒血症出现在感染后2天,随之在脑、肺、肝、肾和脾脏中查到。以上结果表明:EHF病毒对乳鼠具有广泛的嗜性,脑内感染途径能获得较高滴度的感染性病毒。提示EHF病毒在鼠mφ中增殖并携带至全身播散,可能是造成乳鼠全身性弥漫性感染的主要原因。     

含HTNV S基因重组痘苗病毒在HFRS患者B淋巴母细胞系中的表达

采用Ficoll密度梯度离心法(淋巴细胞分离液)分离肾综合征出血热(HFRS)患者外周血单个核细胞（PBMC),并用EB病毒(EBV)感染B淋巴细胞,建立永生化的B淋巴母细胞系(B—LCL)。然后,用含汉滩病毒(Hantaan virus,HTNV)S基因的重组痘苗病毒感染B—LCL,应用问接免疫荧光检测核衣壳蛋白的表达。结果表明,B淋巴细胞经EBV感染4周左右,可形成永生化B—LCL。成功转化后的B—LCL,体积增大,且增殖的淋巴细胞积聚成团。汉滩病毒S基因在B—LCL中能有效表达核衣壳蛋白。含S基因的重组痘苗病毒感染的B—LCL可用作HTNV核衣壳蛋白特异性CTL活性研究的靶细胞。     

The effect of α-MSH on fever caused by Staphylococcus aureus cell walls in rabbits

The role of endogenous pyrogens induced by gram-positive bacterial pyrogens is not known. Intravenous alpha-MSH (2.5 micrograms) significantly reduced only the first phase of the biphasic thermal response to IV S. aureus cell walls (5 x 10(7)). Intracerebroventricular alpha-MSH (200 ng) had no effect on the fever response. The fall in serum iron concentration was significantly attenuated by the IV alpha-MSH but was not affected by the ICV alpha-MSH. Intravenous alpha-MSH had no effect on fever or the serum iron response caused by muramyl dipeptide (MDP). We conclude that the first phase of the thermal response to S. aureus cell walls is mediated by an endogenous pyrogen (EP) and the second phase of the response by a mechanism not involving EP, but possibly a muramyl peptide.     

The Genomic Blueprint of Salmonella enterica subspecies enterica serovar Typhi P-stx-12

Salmonella enterica subspecies enterica serovar Typhi is a rod-shaped, Gram-negative, facultatively anaerobic bacterium. It belongs to the family Enterobacteriaceae in the class Gammaproteobacteria, and has the capability of residing in the human gallbladder by forming a biofilm and hence causing the person to become a typhoid carrier. Here we present the complete genome of Salmonella enterica subspecies enterica serotype Typhi strain P-stx-12, which was isolated from a chronic carrier in Varanasi, India. The complete genome comprises a 4,768,352 bp chromosome with a total of 98 RNA genes, 4,691 protein-coding genes and a 181,431 bp plasmid. Genome analysis revealed that the organism is closely related to Salmonella enterica serovar Typhi strain Ty2 and Salmonella enterica serovar Typhi strain CT18, although their genome structure is slightly different.