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261.
Plant–herbivore interactions have strong ecological and evolutionary consequences, but have been traditionally overlooked
in marine higher plants. Despite recent advances in seagrass ecology that highlight the importance of herbivory, the mechanisms
that regulate the feeding behaviour of seagrass consumers remain largely unknown. Herbivores have been shown to reduce the
sexual reproductive success of seagrasses through direct consumption of inflorescences and seeds, but we know little about
intraspecific variation in susceptibility to grazing of different seagrass tissues. We contrasted the relative palatability
of reproductive and vegetative tissues of the temperate seagrass Posidonia oceanica in the field, and we assessed the feeding preferences among these tissues of the main consumers of the plant, the fish Sarpa salpa and the urchin Paracentrotus lividus. Moreover, we identified the plant traits that explained the observed feeding behaviour. We provide strong evidence for herbivore
selectivity among seagrass tissues. In the field, 70–90% of inflorescences were damaged by herbivores compared to 3–60% of
leaves of similar age. In feeding assays, the urchin P. lividus showed over a twofold preference for reproductive tissue at various stages of development. By contrast, we detected no feeding
activity on either leaves or inflorescences from the fish S. salpa, which is known to migrate to deeper waters soon after flowering starts and during the period of fruit maturation. Despite
being the preferred food of urchins, inflorescences were chemically defended, had higher levels of phenolics and lower nutrient
and calorific content than leaves. We experimentally demonstrated that leaf structural defences are the primary factor in
determining urchin feeding preferences. Removal of plant structure results in a drastic shift in urchin selectivity towards
the most nutritious and less chemically defended leaf tissue, indicating that multiple mechanisms of defence to herbivory
may coexist in seagrasses. 相似文献
262.
The Belize atolls—Glovers Reef, Lighthouse Reef and Turneffe Islands—show differences in geomorphology, lagoonal depth, bottom
sediment, growth of mangroves and sea-grass, exposure to waves and currents as well as in their sedimentation rates and their
age. Bivalve shell assemblages in lagoonal areas reflect these geomorphological differences. On each atoll, 32 to 44 recent
sediment samples were taken (total number of samples 111) and bivalve shells subsequently identified. The resulting database
(32,122 bivalve shells in total) was analysed using Q-mode cluster analyses. Both the distribution of species characteristic
of different lagoonal habitats and the distribution of bivalves with different life and feeding habits were investigated.
Epifaunal suspension feeders were found particularly on hard-bottom along the reef-crests or clinging to mangrove roots. Infaunal
suspension feeders show a more diverse distribution. Deeper lagoonal parts and areas with mangrove growth are often inhabited
by chemosymbiont-carrying bivalves, indicating locations of reduced sediment. Deep burrowing detritus feeders are very abundant
in shallow-water areas with moderate to high water agitation and were seldom found in Halimeda-rich sediments. 相似文献
263.
Taylor JR Whittamore JM Wilson RW Grosell M 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2007,177(6):597-608
The effects of feeding on both acid–base and ion exchange with the environment, and internal acid–base and ion balance, in
freshwater and seawater-acclimated flounder were investigated. Following voluntary feeding on a meal of 2.5–5% body mass and
subsequent gastric acid secretion, no systemic alkaline tide or respiratory compensation was observed in either group. Ammonia
efflux rates more than doubled from 489 ± 35 and 555 ± 64 μmol kg−1 h−1 under control conditions to 1,228 ± 127 and 1,300 ± 154 μmol kg−1 h−1 post-feeding in freshwater and seawater-acclimated fish, respectively. Based on predictions of gastric acid secreted during
digestion, we calculated net postprandial internal base gains (i.e., HCO3− secreted from gastric parietal cells into the blood) of 3.4 mmol kg−1 in seawater and 9.1 mmol kg−1 in freshwater-acclimated flounder. However, net fluxes of ammonia, titratable alkalinity, Na+ and Cl− indicated that branchial Cl−/HCO3− and Na+/H+ exchange played minimal roles in counteracting these predicted base gains and cannot explain the absence of alkaline tide.
Instead, intestinal Cl−/HCO3− exchange appears to be enhanced after feeding in both freshwater and seawater flounder. This implicates the intestine rather
than the gills as a potential route of postprandial base excretion in fish, to compensate for gastric acid secretion. 相似文献
264.
<Emphasis Type="Italic">Bacillus megaterium</Emphasis>—from simple soil bacterium to industrial protein production host 总被引:2,自引:0,他引:2
Vary PS Biedendieck R Fuerch T Meinhardt F Rohde M Deckwer WD Jahn D 《Applied microbiology and biotechnology》2007,76(5):957-967
Bacillus megaterium has been industrially employed for more than 50 years, as it possesses some very useful and unusual enzymes and a high capacity
for the production of exoenzymes. It is also a desirable cloning host for the production of intact proteins, as it does not
possess external alkaline proteases and can stably maintain a variety of plasmid vectors. Genetic tools for this species include
transducing phages and several hundred mutants covering the processes of biosynthesis, catabolism, division, sporulation,
germination, antibiotic resistance, and recombination. The seven plasmids of B. megaterium strain QM B1551 contain several unusual metabolic genes that may be useful in bioremediation. Recently, several recombinant
shuttle vectors carrying different strong inducible promoters and various combinations of affinity tags for simple protein
purification have been constructed. Leader sequences-mediated export of affinity-tagged proteins into the growth medium was
made possible. These plasmids are commercially available. For a broader application of B. megaterium in industry, sporulation and protease-deficient as well as UV-sensitive mutants were constructed. The genome sequence of
two different strains, plasmidless DSM319 and QM B1551 carrying seven natural plasmids, is now available. These sequences
allow for a systems biotechnology optimization of the production host B. megaterium. Altogether, a “toolbox” of hundreds of genetically characterized strains, genetic methods, vectors, hosts, and genomic sequences
make B. megaterium an ideal organism for industrial, environmental, and experimental applications. 相似文献
265.
Weibo Dong C. Corley Holbrook Patricia Timper Timothy B. Brenneman Benjamin G. Mullinix 《Journal of nematology》2007,39(2):169-175
Use of resistant cultivars is a desirable approach to manage the peanut root-knot nematode (Meloidogyne arenaria). To incorporate resistance into commercially acceptable cultivars requires reliable, efficient screening methods. To optimize the resistance screening protocol, a series of greenhouse tests were done using seven genotypes with three levels of resistance to M. arenaria. The three resistance levels could be separated based on gall indices as early as two weeks after inoculation (WAI) using 8,000 eggs of M. arenaria per plant, while four or more weeks were needed when 1,000–6,000 eggs/plant were used. High inoculum densities (over 8,000 eggs/plant) were needed to separate the three resistance levels based on eggs per gram of root within eight WAI. A gall index based on percentage of galled roots could separate the three resistance levels at lower inoculum levels and earlier harvest dates than other assessment methods. The use of eggs vs. second-stage juveniles (J2) as inoculum provided similar results; however, it took three to five more days to collect J2 than to collect eggs from roots. Plant age affected gall index and nematode reproduction on peanut, especially on the susceptible genotypes AT201 and D098. The genotypes were separated into their correct resistance classes when inoculated 10 to 30 days after planting, but were not separated correctly when inoculated on day 40. 相似文献
266.
Devin A. Lyons Robert E. Scheibling 《Journal of experimental marine biology and ecology》2007,349(1):194-204
Feeding behaviour is influenced by a variety of factors, including nutritional requirements, the quality of available foods, and environmental conditions. We examined the effect of two factors, food morphology and dietary history, on the feeding rate and preference of the sea urchin Strongylocentrotus droebachiensis. Standardizing food shape and structure did not alter urchins' expected preference for the native kelp Laminaria longicruris over the invasive alga Codium fragile ssp. tomentosoides. However, when foods containing L. longicuris were shaped to mimic the algae, the C. fragile mimic was consumed more rapidly than the kelp mimic. Dietary history had no effect on single diet feeding rate. Urchins feeding on C. fragile consistently consumed twice as much (by mass) as those fed kelp, regardless of their previous diet. Despite higher feeding rates on C. fragile, urchins feeding on this alga were unable to compensate for its low energetic content and ingested less energy. Dietary history had a short-term effect on food preference, with urchins tending to prefer less familiar foods. Our findings suggest that urchins feed on C. fragile at a high rate, due to ease of handling and/or compensatory feeding, and that they do not a have strict preference hierarchy. Rather, food choice appears to reflect active maintenance of a mixed diet. 相似文献
267.
We studied foraging site partitioning between the sexes in Neolamprologus tetracanthus, a shrimp-eating Tanganyikan cichlid with harem-polygyny. Females maintained small territories against heterospecific food
competitors within large territories of males, foraging exclusively at the inner side of their own territories (foraging areas).
Males fed as frequently as females in their own territories, but mostly outside female foraging areas, although they frequently
entered female territories and repelled food competitors from the territories. Soon after removal of the resident females,
however, harem males, as well as many food competitors, invaded the vacant territories and intensively devoured prey of female
foraging areas. This indicates that although female foraging areas appear to contain more food than outside the areas, harem
males refrained from foraging there when the resident females were present. We suggest that harem males will attempt to keep
female foraging areas in good condition, whereby they may get females to reside in male territories and/or promote female
gonadal maturation. 相似文献
268.
Melittobia digitata (Hymenoptera: Eulophidae) are parasitoids known for their cooperative escape behavior. The initial sequences of this escape
chewing behavior have been compared to the initial sequences of their feeding behavior. We sought to experimentally test whether
these sequences were interchangeable. We were successful in turning off chewing and turning on feeding in a chewing situation,
and turning on chewing and turning off feeding in a feeding context. We discuss the implications of this finding for the origin
and evolution of these behaviors, and how experimental tests of key cues can be helpful for understanding the evolution of
behavioral patterns. 相似文献
269.
The Tat system has the remarkable capacity of exporting proteins in folded conformation across the cytoplasmic membrane. The functional Tat translocase from Gram-negative bacteria consists of TatA, TatB and TatC proteins. To gain information about the species specificity of the Tat translocase, we cloned tat genes from Gram-negative pathogens Shigella flexneri 2a str. 301, Vibrio cholerae El Tor N16961, Pseudomonas aeruginosa PAO1, thermophilic Sulfolobus solfataricus P2, Thermus thermophilus HB8 and from three Magnetospirillum species (AMB-1, MS-1 and MSR-1), and assessed the capacity of these Tat systems to restore the Tat-dependent growth defect of Escherichia coli tat mutants. We found that whereas the tat genes from the thermophilic bacterial and archaeal species were not functional in E. coli, other tat genes could all complement the phenotype of the E. coli tat mutants. In addition, a chimera composed of the N-terminus of V. cholerae TatE and C-terminus of M. magneticum TatA was functional. Whereas the expression of the tatABC genes from P. aeruginosa and Magnetospirillum strains must be induced to obtain a functional Tat system, overproduction of the V. cholerae TatABC proteins abolished the complementation. The complementation impairment seemed to be correlated with increasing level of slow-migrating TatC isoforms. In vitro studies showed that slow-migrating TatC isoforms in the purified V. cholerae TatABC complex increased with storage time. Together these results showed that the Tat translocases from the Gram-negative bacteria are generally functional in E. coli and the expression level is crucial for in vivo reconstitution of a functional Tat translocase. 相似文献
270.