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91.
Delano V. Young Michael C. Dean Peter Heit Stewart D. Chipman 《In vitro cellular & developmental biology. Plant》1980,16(11):949-957
Summary Simian virus 40-transformed 3T3 cells are dependent on serum for survival and growth. This growth activity can be separated
on a pH 2 Sephadex G100 column into two fractions: a high molecular weight activity and a low molecular weight substance that
has recently been characterized as containing as its major agent, biotin.
To replace the remainder of the serum requirement, hormones and other growth factors were tested. Both insulin at high, nonphysiological
concentrations (200 to 500 ng/ml) and transferrin (5×10−8
M) stimulate the growth rate in low serum medium (0.3% v/v bovine calf serum DME) individually and, when added together, are
nearly as growth enhancing as 10% serum.
The need for the residual serum in this medium can be eliminated by the use of crystalline trypsin during trypsinization.
Under these serum-free conditions, biotin and transferrin supplementation provide for moderately good growth (20 to 30 hr
population doubling time, 1×106 cells/3.2-cm dish final cell density). Insulin addition further stimulates the growth rate (16 to 20 hr) and the final density
(1.5×106 cells). Although the protein growth factors, EGF (0.5 to 1.0 ng/ml) and FGF (4 to 10 ng/ml), also appear to enhance growth
individually and additively, their effects are slight and very variable. Nevertheless, the complete serum-free medium (DME
supplemented with biotin, transferrin, insulin, EGF and FGF) yields growth comparable but still inferior to 10% serum supplementation
(14-versus 12-hr population doubling time, 1 to 2×106 versus 2 to 3×106 cells final cell density).
This work was supported by NIH Grant CA 20040. 相似文献
92.
The dose-dependent effect of intravenously infused synthetic somatostatin-14 on basal and postprandial insulin and gastrin release was assessed in anesthetized rats.Infusion of 1 ng · kg?1 · min?1 elicited a significant reduction of basal and postprandial insulin levels compared to the saline control group. At 15 ng · kg?1 · min?1 basal insulin was not affected but postprandial insulin levels were still significantly reduced. At 30 ng · kg?1 · min?1 neither basal nor stimulated insulin levels were affected. At the highest concentration of 120 ng · kg?1 · min?1 basal and postprandial insulin levels were suppressed similar to the lowest infusion rate of 1 ng · kg?1 · min?1. Basal gastrin levels were significantly reduced only at the highest rate of 120 ng · kg?1 · min?1. A significant reduction of postprandial gastrin levels was observed at 15 ng · kg?1 · min?1 and all higher infusion rates employed. Measurements of plasma somatostatin-like immunoreactivity (SLI) demonstrated that plasma SLI levels during the lowest infusion rate of 1 ng · kg?1 · min?1 were not different from the controls. No significant rise of plasma SLI levels was observed in response to the test meal. The higher infusion rates elicited a dose-dependent increase in plasma SLI levels. These data demonstrate that in rats somatostatin exerts a biological effect on insulin release at very low doses while certain greater infusion rates have no suppressive effect. Gastrin secretion is inhibited in a more linear pattern. 相似文献
93.
Brian A. Laishes Gary M. Williams 《In vitro cellular & developmental biology. Plant》1976,12(7):521-532
Summary The conditions for obtaining representative, primary adult rat hepatocyte cultures were explored. The methods applied included
enzymatic liver perfusion which was nondestructive to hepatocytes, the prevention of aggregation of dissociated cells and
the selective attachment of viable cells. These procedures yielded a recovery of 50% of the liver cells which gave rise to
cultures representing 14% of the total liver cells. The cultures were composed of homogeneous epithelial-like cells cytologically
similar to hepatocytes and possessed a number of liver-specific enzymes. There was virtually no cell division initially and
most cells died between 24 and 48 hr. Insulin enhanced the attachment of the liver cells, altered their morphology, but did
not prolong cell survival.
This study was supported by grant no. BC 133 from the American Cancer Society. 相似文献
94.
95.
《Chronobiology international》2013,30(3):225-228
Four healthy non obese young volunteers were observed for a 24-hr period, every other month, over the course of one year. Tolbutamide was injected i.v. each day of the experiment every four hours. Tolbutamide-induced insulin secretion (T.I.I.S.) was evaluated by planimetrically measuring insulin areas above basal levels. Tolbutamide-induced hypoglycemic effect was evaluated by measuring the blood glucose difference between the Sth and 25th minute after the drug injection (δG 5′-25′). The macroscopic evaluation of T.I.I.S. and δG 5′-2S′(mean chronograms) permitted the detection of the existence of a circannual variation of both variables. In particular the maximum level of the blood glucose drop (δG 5-25) was registered in February.Subsequently the quantification of the rhythm of T.I.I.S. was obtained by fitting a sine curve, according to the Cosinor method. The highest insulin release was confirmed in winter.As previously documented, the existence of a statistically significant circadian rhythm of T.I.I.S. was confirmed in the morning, i.e. the same period of the day in which insulin-induced hypoglycemia occurs. 相似文献
96.
97.
《Animal : an international journal of animal bioscience》2020,14(8):1676-1683
Improving reproductive performance is one of the most important factors affecting the profitability of dairy herds. This study investigated the effect of feeding a high starch (HS) diet and body condition score (BCS) at calving on blood metabolites, fertility and ovarian function and milk production in Holstein dairy cows. One hundred seventy-four multiparous cows were fed common close-up and early lactation diets during the first 15 days in milk (DIM). Cows were randomly assigned to 1 of 2 experimental diets from 16 until 50 DIM (n = 87 per group); normal starch (228 g/kg diet DM; NS) or HS (270 g/kg diet DM; HS) diets. Each treatment group was further subdivided based on BCS at calving as normal BCS (BCS ⩽ 3.5; normal BCS (NBCS); n = 45) or high BCS (HBCS) (BCS ⩾ 3.75; HBCS; n = 42). A significant difference was detected for increased milk production (47.24 v. 44.55 kg/day) and decreased milk fat (33.93 v. 36.33 g/kg) in cows fed HS or NS, respectively. Plasma glucose and insulin concentrations were significantly higher in cows fed the HS compared to the NS diet. Diets significantly affected DIM at first artificial insemination (AI, 79.51 ± 3.83 v. 90.40 ± 3.83 days for cows fed HS and NS diets, respectively). High BCS groups had greater milk fat content and elevated plasma nonesterified fatty acids (NEFA), β hydroxybutyrate (BHB) and bilirubin concentrations. In general, feeding higher starch diets to normal BCS cows during the first 50 DIM improved productive and reproductive performance of early-lactating dairy cows. 相似文献
98.
《Animal : an international journal of animal bioscience》2020,14(12):2523-2534
Sub-acute ruminal acidosis (SARA) is sometimes observed along with reduced milk fat synthesis. Inconsistent responses may be explained by dietary fat levels. Twelve ruminally cannulated cows were used in a Latin square design investigating the timing of metabolic and milk fat changes during Induction and Recovery from SARA by altering starch levels in low-fat diets. Treatments were (1) SARA Induction, (2) Recovery and (3) Control. Sub-acute ruminal acidosis was induced by feeding a diet containing 29.4% starch, 24.0% NDF and 2.8% fatty acids (FAs), whereas the Recovery and Control diets contained 19.9% starch, 31.0% NDF and 2.6% FA. Relative to Control, DM intake (DMI) and milk yield were higher in SARA from days 14 to 21 and from days 10 to 21, respectively (P < 0.05). Milk fat content was reduced from days 3 to 14 in SARA (P < 0.05) compared with Control, while greater protein and lactose contents were observed from days 14 to 21 and 3 to 21, respectively (P < 0.05). Milk fat yield was reduced by SARA on day 3 (P < 0.05), whereas both protein and lactose yields were higher on days 14 and 21 (P < 0.05). The ruminal acetate-to-propionate ratio was lower, and the concentrations of propionate and lactate were higher in the SARA treatment compared with Control on day 21 (P < 0.05). Plasma insulin increased during SARA, whereas plasma non-esterified fatty acids and milk β-hydroxybutyrate decreased (P < 0.05). Similarly to fat yield, the yield of milk preformed FA (>16C) was lower on day 3 (P < 0.05) and tended to be lower on day 7 in SARA cows (P < 0.10), whereas yield of de novo FA (<16C) was higher on day 21 (P < 0.01) in the SARA group relative to Control. The t10- to t11-18:1 ratio increased during the SARA Induction period (P < 0.05), but the concentration of t10-18:1 remained below 0.5% of milk fat, and t10,c12 conjugated linoleic acid remained below detection levels. Odd-chain FA increased, whereas branched-chain FA was reduced during SARA Induction from days 3 to 21 (P < 0.05). Sub-acute ruminal acidosis reduced milk fat synthesis transiently. Such reduction was not associated with ruminal biohydrogenation intermediates but rather with a transient reduction in supply of preformed FA. Subsequent rescue of milk fat synthesis may be associated with higher availability of substrates due to increased DMI during SARA. 相似文献
99.
Nikkhil Velingkaar Volha Mezhnina Allan Poe Kuldeep Makwana Richa Tulsian Roman V. Kondratov 《Aging cell》2020,19(4)
Caloric restriction (CR) has positive effects on health and longevity. CR in mammals implements time‐restricted (TR) feeding, a short period of feeding followed by prolonged fasting. Periodic fasting, in the form of TR or mealtime, improves metabolism without reduction in caloric intake. In order to understand the relative contribution of reduced food intake and periodic fasting to the health benefits of CR, we compared physiological and metabolic changes induced by CR and TR (without reduced food intake) in mice. CR significantly reduced blood glucose and insulin around the clock, improved glucose tolerance, and increased insulin sensitivity (IS). TR reduced blood insulin and increased insulin sensitivity, but in contrast to CR, TR did not improve glucose homeostasis. Liver expression of circadian clock genes was affected by both diets while the mRNA expression of glucose metabolism genes was significantly induced by CR, and not by TR, which is in agreement with the minor effect of TR on glucose metabolism. Thus, periodic fasting contributes to some metabolic benefits of CR, but TR is metabolically different from CR. This difference might contribute to differential effects of CR and TR on longevity. 相似文献
100.
Raju Nagaraju Apurva K. R. Joshi Sowmya G. Vamadeva Padmanabhan S. Rajini 《Journal of biochemical and molecular toxicology》2020,34(8)
In our previous study, we demonstrated the potential of monocrotophos (MCP), an organophosphorus insecticide (OPI), to induce glucose intolerance, insulin resistance (IR), and dyslipidemia with hyperinsulinemia in rats after chronic exposure. As hyperinsulinemia is likely to exert an impact on hepatic lipid metabolism, we carried out this study to establish the effect of chronic MCP exposure (0.9 and 1.8 mg/kg/day for 180 days) on hepatic lipid metabolism in rats. The state of IR induced by MCP in rats was associated with an increase in the liver lipid content (triglyceride and cholesterol) and expression levels of sterol regulatory element‐binding proteins, PPARγ, acetyl‐CoA carboxylase, and fatty acid synthase in the liver. Similarly, activities of key enzymes (acetyl‐COA carboxylase, fatty acid synthase, lipin 1, malic enzyme, glucose‐6‐phosphate dehydrogenase, and glycerol‐3‐phosphate dehydrogenase), which regulate lipogenesis, were enhanced in livers of pesticide‐treated rats. A strong correlation was observed between insulin levels, hepatic lipid content, and plasma lipid profile in treated rats. Our study suggests that long‐term exposure to OPIs not only has a propensity to induce a state of hyperinsulinemic IR, but it is also associated with augmented hepatic lipogenesis, which may explain dyslipidemia induced by chronic exposure to MCP. 相似文献