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21.
Cultivable bacterial diversity from the human colon 总被引:1,自引:0,他引:1
Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of the composition and diversity of the colonic microbiota. Importantly, however, it is the continued isolation of bacterial representatives of key groups that offers the best opportunity to conduct detailed metabolic and functional studies. This also permits bacterial genome sequencing which will accelerate the linkage to functionality. Obtaining new human colonic bacterial isolates can be challenging, because most of these are strict anaerobes and many have rather exact nutritional and physical requirements. Despite this many new species are being isolated and described that occupy distinct niches in the colonic microbial community. This review focuses on these under-studied yet important gut anaerobes. 相似文献
22.
Sokolova T Hanel J Onyenwoke RU Reysenbach AL Banta A Geyer R González JM Whitman WB Wiegel J 《Extremophiles : life under extreme conditions》2007,11(1):145-157
Three thermophilic strains of chemolithoautotrophic Fe(III)-reducers were isolated from mixed sediment and water samples (JW/KA-1 and JW/KA-2(T): Calcite Spring, Yellowstone N.P., WY, USA; JW/JH-Fiji-2: Savusavu, Vanu Levu, Fiji). All were Gram stain positive rods (approximately 0.5 x 1.8 microm). Cells occurred singly or in V-shaped pairs, and they formed long chains in complex media. All utilized H(2) to reduce amorphous iron (III) oxide/hydroxide to magnetite at temperatures from 50 to 75 degrees C (opt. approximately 73 degrees C). Growth occurred within the pH(60C) range of 6.5-8.5 (opt. pH(60C) 7.1-7.3). Magnetite production by resting cells occurred at pH(60C) 5.5-10.3 (opt. 7.3). The iron (III) reduction rate was 1.3 mumol Fe(II) produced x h(-1) x ml(-1) in a culture with 3 x 10(7) cells, one of the highest rates reported. In the presence or absence of H(2), JW/KA-2(T) did not utilize CO. The G + C content of the genomic DNA of the type strain is 52.7 +/- 0.3 mol%. Strains JW/KA-1 and JW/KA-2(T) each contain two different 16S rRNA gene sequences. The 16S rRNA gene sequences from JW/KA-1, JW/KA-2(T), or JW/JH-Fiji-2 possessed >99% similarity to each other but also 99% similarity to the 16S rRNA gene sequence from the anaerobic, thermophilic, hydrogenogenic CO-oxidizing bacterium 'Carboxydothermus restrictus' R1. DNA-DNA hybridization between strain JW/KA-2(T) and strain R1(T) yielded 35% similarity. Physiological characteristics and the 16S rRNA gene sequence analysis indicated that the strains represent two novel species and are placed into the novel genus Thermolithobacter within the phylum 'Firmicutes'. In addition, the levels of 16S rRNA gene sequence similarity between the lineage containing the Thermolithobacter and well-established members of the three existing classes of the 'Firmicutes' is less than 85%. Therefore, Thermolithobacter is proposed to constitute the first genus within a novel class of the 'Firmicutes', Thermolithobacteria. The Fe(III)-reducing Thermolithobacter ferrireducens gen. nov., sp. nov. is designated as the type species with strain JW/KA-2(T) (ATCC 700985(T), DSM 13639(T)) as its type strain. Strain R1(T) is the type strain for the hydrogenogenic, CO-oxidizing Thermolithobacter carboxydivorans sp. nov. (DSM 7242(T), VKM 2359(T)). 相似文献
23.
We examined the effects of arbuscular mycorrhizas (AM), phosphorus fertilization, intraspecific density, and their interaction, on the growth, phosphorus uptake, and root morphology of three facultative mycotrophic crops (Capsicum annuum, Zea mays, and Cucurbita pepo). Plants were grown in pots with or without AM at three densities and four phosphorus availabilities for 10 weeks. AM colonization, plant weight, and shoot phosphorus concentration were measured at harvest. Root morphology was assessed for C. annuum and Z. mays. Phosphorus fertilization reduced but did not eliminate AM colonization of all species. AM, phosphorus, and density interacted significantly to modify growth of C. annuum and C. pepo such that increased density and phosphorus diminished beneficial effects of AM. Increased density reduced positive effects of AM on C. annuum and C. pepo shoot phosphorus concentrations. AM altered both Z. mays and C. annuum root morphology in ways that complemented potential phosphorus uptake by mycorrhizas, but increased density and phosphorus diminished these effects. We infer that increased density predominantly influenced plant responses by affecting whether or not carbon (photosynthate) or phosphorus limited plant growth. By exacerbating carbon limitation, high density reduced the benefit/cost ratio of mycorrhizas and minimized their effects. 相似文献
24.
Catalase and Superoxide Dismutase: Distribution, Properties, and Physiological Role in Cells of Strict Anaerobes 总被引:3,自引:0,他引:3
This review considers the distribution of the main enzymes of antioxidative defense, superoxide dismutase (SOD) and catalase, in various groups of strictly anaerobic microorganisms: bacteria of the genus Clostridium, Bacteroides, sulfate-reducing and acetogenic bacteria, methanogenic archaea, etc. Molecular and biochemical properties of purified Fe-containing SODs, cambialistic SODs, and heme catalases are presented. The physiological role and origin of the enzymes of antioxidative defense in strict anaerobes are discussed. Physiological responses (induction of SOD and catalase) to factors provoking oxidative stress in the cells of strict anaerobes able to maintain viability under aerobic conditions are also considered. 相似文献
25.
We investigated the influence of inhibitors of energy metabolism and ionophores on the growth and formation of metabolic products in alkaliphilic anaerobes characterized by various catabolism types. It was shown that blockage of oxidative phosphorylation by the addition of N,N-dicyclohexylcarbodiimide (DCCD), an inhibitor of F1F0 ATP synthase, resulted in a complete arrest of the growth of the acetogenic bacterium Tindallia
magadiensis with arginine as an electron acceptor. In the presence of pyruvate, substrate-level phosphorylation occurred. The methylotrophic methanogenic archaebacterium Methanosalsus
zhilinae did not grow with DCCD and vanadate, an inhibitor of 12 ATPase, suggesting the presence of two ATPase types in this species. In the saccharolytic alkaliphiles Halonatronum saccharophilum, Amphibacillus tropicus, and Spirochaeta alkalica (which are characterized by different pH optima), the contribution of the H+ gradient to the energy metabolism and, presumably, to the maintenance of the intracellular pH level decreased with an increase in the degree of alkaliphily. Based on the data of an inhibitor assay using protonophores, monensin, and amiloride, we suggest that all of the bacteria tested depend on H+and Na+ gradients. The Na+/H+ antiport appears to be a universal mechanism of regulating the intracellular pH level and the interaction between the Na+ and H+ cycles in bacterial cells cultivated under alkaline conditions. 相似文献
26.
Hypoxic/necrotic regions, absent in normal tissues, can be exploited to target tumours in cancer therapy using nonpathogenic strains of the bacterial genus Clostridium. Following administration of Clostridium spores to tumour-bearing organisms, these spores can only germinate within the hypoxic/necrotic regions of solid tumours, proving their exquisite selectivity. Low oxygen tension is a common feature of solid tumours, which may arise from the unique physiological environment, generated to a large extent by the abnormal tumour vasculature, and provides as such a niche for anaerobic bacteria. Some clostridia tested clearly showed innate oncolytic activity, but they could not completely eradicate the tumour. Recombinant clostridia producing prodrug-converting enzymes or cytokines resulted in the production of such proteins solely within the tumour, and where applicable, could convert the prodrug in a toxic compound. Moreover, in some cases, tumour eradication or tumour control could be observed. This review brings an overview of the relative successes and failures of the Clostridium-directed tumour therapy with both wild-type strains and strains producing proteins useful in antitumour therapy. 相似文献
27.
Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon
The release of energy from particulate substrates such as dietary fiber and resistant starch (RS) in the human colon may depend on the presence of specialist primary degraders (or ‘keystone species'') within the microbial community. We have explored the roles of four dominant amylolytic bacteria found in the human colon in the degradation and utilization of resistant starches. Eubacterium rectale and Bacteroides thetaiotaomicron showed limited ability to utilize RS2- and RS3-resistant starches by comparison with Bifidobacterium adolescentis and Ruminococcus bromii. In co-culture, however, R. bromii proved unique in stimulating RS2 and RS3 utilization by the other three bacterial species, even in a medium that does not permit growth of R. bromii itself. Having previously demonstrated low RS3 fermentation in vivo in two individuals with undetectable populations of R. bromii-related bacteria, we show here that supplementation of mixed fecal bacteria from one of these volunteers with R. bromii, but not with the other three species, greatly enhanced the extent of RS3 fermentation in vitro. This argues strongly that R. bromii has a pivotal role in fermentation of RS3 in the human large intestine, and that variation in the occurrence of this species and its close relatives may be a primary cause of variable energy recovery from this important component of the diet. This work also indicates that R. bromii possesses an exceptional ability to colonize and degrade starch particles when compared with previously studied amylolytic bacteria from the human colon. 相似文献
28.
Factors affecting the bionomics of the trichogrammatid egg parasitoid Lathromeris ovicida and its association with the two sympatric scelionid egg parasitoids Telenomus busseolae and Telenomus isis were studied in the laboratory. The potential host species employed were the noctuids Sesamia calamistis and Sesamia botanephaga, and the pyralids Eldana saccharina and Mussidia nigrivenella. Using eggs of S. calamistis Hampson as hosts, the lower and upper thresholds for development were estimated at 14.8 and 34 °C, respectively. The percent parasitism, total progeny, and sex ratio decreased gradually from 42.0 to 10.2, from 56.4 to 10.8, and from 0.74 to 0.61, respectively, with host age. The intrinsic rate of increase and female fecundity was highest on the two noctuids, followed by E. saccharina and M. nigrivenella. Lathromeris ovicida preferentially attacked eggs already parasitised by either Telenomus busseolae or Telenomus isis. Thus, L. ovicida is probably a facultative hyperparasitoid. 相似文献
29.
Strictly anaerobic microorganisms relating to various physiological groups were screened for catalase and superoxide dismutase (SOD) activity. All of the investigated anaerobes possessed SOD activity, necessary for protection against toxic products of oxygen reduction. High specific activities of SOD were found in Acetobacterium woodii and Acetobacterium wieringae. Most of the investigated clostridia and acetogens were catalase-negative. A significant activity of catalase was found in Thermohydrogenium kirishiense, in representatives of the genus Desulfotomaculum, and in several methanogens. Methanobrevibacter arboriphilus had an exceptionally high catalase activity after growth in medium supplemented with hemin. Hemin also produced a strong positive effect on the catalase activity in many other anaerobic microorganisms. In methanogens, the activities of the enzymes of antioxidant defense varied in wide ranges depending on the stage of growth and the energy source. 相似文献
30.