Bioinformatic analysis of BBTV satellite DNA in Hainan

Banana bunchy top virus (BBTV),family Nanaviridae,genus Babuvirus,is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants.It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region.In this study we isolated,cloned and sequenced a BBTV sample from Hainan Island,China.The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs.We also predicted the physical and chemical properties,structure,signal peptide,phosphorylation,secondary structure,tertiary structure and functional domains of its encoding protein,and compare them with the corresponding quantities in the replication initiation protein of BBTV DNA1.     

供应链上“牛鞭效应”的成因及其有效控制方法分析

许多实证研究与企业调查发现,＂牛鞭效应＂现象广泛存在于供应链结构中.本文首先建立了供应链的经济生态系统模型,并利用该模型充分论证了两点：（1）＂牛鞭效应＂是由供应链自身结构产生的;（2）供应链类似于食物链,它不是契约链,维护供应关系健康持续发展的有效措施是,降低供求双方的依赖程度,而不是通过激励的途径促进供应端企业的供应.这两个结论有别于通常供应链研究的结果,所以本文的结论有益于供应链的理论研究与实践应用.     

Mechanistic evaluation of virus clearance by depth filtration

Virus clearance by depth filtration has not been well‐understood mechanistically due to lack of quantitative data on filter charge characteristics and absence of systematic studies. It is generally believed that both electrostatic interactions and sized based mechanical entrapment contribute to virus clearance by depth filtration. In order to establish whether the effectiveness of virus clearance correlates with the charge characteristics of a given depth filter, a counter‐ion displacement technique was employed to determine the ionic capacity for several depth filters. Two depth filters (Millipore B1HC and X0HC) with significant differences in ionic capacities were selected and evaluated for their ability to eliminate viruses. The high ionic capacity X0HC filter showed complete porcine parvovirus (PPV) clearance (eliminating the spiked viruses to below the limit of detection) under low conductivity conditions (≤2.5 mS/cm), achieving a log₁₀ reduction factor (LRF) of > 4.8. On the other hand, the low ionic capacity B1HC filter achieved only ～2.1–3.0 LRF of PPV clearance under the same conditions. These results indicate that parvovirus clearance by these two depth filters are mainly achieved via electrostatic interactions between the filters and PPV. When much larger xenotropic murine leukemia virus (XMuLV) was used as the model virus, complete retrovirus clearance was obtained under all conditions evaluated for both depth filters, suggesting the involvement of mechanisms other than just electrostatic interactions in XMuLV clearance. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 31:431–437, 2015     

阿奇霉素联合辛伐他汀对慢性阻塞性肺疾病合并肺动脉高压患者肺功能的影响

目的:探讨阿奇霉素联合辛伐他汀治疗慢性阻塞性肺疾病合并肺动脉高压的临床疗效及对患者肺功能的影响。方法:选取2013年6月-2016年3月我院收治的慢性阻塞性肺疾病合并肺动脉高压患者107例,根据治疗方法不同分为对照组(49例)与实验组(58例)。对照组患者采用阿奇霉素治疗,实验组患者在对照组基础上给予辛伐他汀治疗。观察并比较两组患者的临床疗效、不良反应以及肺功能指标的变化情况。结果:实验组患者治疗有效率(87.93%)高于对照组(73.47%),差异具有统计学意义(P0.05)。与治疗前比较,两组患者治疗后1 s用力呼吸容积(FEV1)、用力肺活量(FVC)及FEV1/FVC水平均升高,差异具有统计学意义(P0.05);与对照组比较,实验组患者治疗后1 s用力呼吸容积(FEV1)、用力肺活量(FVC)及FEV1/FVC水平较高,差异具有统计学意义(P0.05)。治疗后,两组患者血清总胆固醇(TC)及三酰甘油(TG)水平均降低,差异具有统计学意义(P0.05);与对照组比较,实验组患者治疗后血清总胆固醇(TC)及三酰甘油(TG)水平较低,差异具有统计学意义(P0.05)。两组患者不良反应发生率比较,差异无统计学意义(P0.05)。结论:阿奇霉素联合辛伐他汀治疗慢性阻塞性肺疾病合并肺动脉高压的临床效果显著,不仅能够改善患者肺功能,降低血脂相关指标水平,并且安全性较高,值得临床推广应用。     

水果中阿斯巴甜的研究

采用高效液相色谱-三重四级杆串联质谱(HPLC-QQQ-MS/MS)对橙等8种水果中的苯丙氨酸、天冬氨酸和阿斯巴甜进行测定,采用气相顶空(HS-GC)对水果中的甲醇进行测定,并进行阿斯巴甜的模拟合成实验,研究在水果中是否存在阿斯巴甜及合成阿斯巴甜所必须的苯丙氨酸、天冬氨酸和甲醇以及它们之间的浓度关系。实验结果表明:8种水果中均含有阿斯巴甜、苯丙氨酸、天冬氨酸和甲醇。其中阿斯巴甜的含量为23.4～117μg/kg,天冬氨酸含量为8.72～186 mg/kg,苯丙氨酸含量为1.84～84.2 mg/kg,甲醇含量为1.81～248mg/kg。不同水果中阿斯巴甜含量差异不大,而苯丙氨酸、天冬氨酸和甲醇的含量差异较大。阿斯巴甜的含量与苯丙氨酸、天冬氨酸和甲醇的含量无明显相关性。     

蛋白激酶C对鼻咽癌细胞c－myc，c－fos表达的影响

用蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ,抑制催化亚基）与Ｓｐｈｉｎｇｏｓｉｎｅ（ＳＳ,抑制调节亚基）处理人鼻咽癌细胞系ＣＮＥ－２Ｚ,经点印迹后扫描定量和免疫细胞化学检测ｃ－ｍｙｃ、ｃ－ｆｏｓ表达及其分布。结果发现：（１）ｃ－ｍｙｃ蛋白：主要分布于胞浆,４０．７％的细胞核与胞浆均为阳性;经ＳＳ或ＳＴ处理后,表达明显减弱,且核阳性率分别为１７．３％与２３．３％。扫描定量在ＳＳ组为对照组的６０％±２５．７％（Ｐ＜０．０５）,ＳＴ组为对照组的５５％±２５．９％（Ｐ＜０．０５）。（２）Ｃ－ｆｏｓ蛋白：主要分布于胞浆,２９．４％的细胞核与胞浆均为阳性,经ＳＳ或ＳＴ处理后,表达明显减弱,且核阳性率分别为９％与１０．２％。扫描定量ＳＳ组为对照组的５８．６％±２５％（Ｐ＜０．０５）,ＳＴ组为对照组的５９．７％±２６．２％（Ｐ＜０．０５）。结果表明：使用ＰＫＣ抑制剂后,两种核癌基因产物量均有不同程度的减少,并且存在细胞内分布的改变,特别是其发挥效应的核内表达明显减少。结合我们以前所发现的ＰＫＣ抑制剂明显抑制ＣＮＥ—２Ｚ细胞生长的结果,提示这些癌基因可能参与了ＰＫＣ对ＣＮＥ—２Ｚ细胞生长的调节。     

白细胞介素2亲和性配体的筛选

白细胞介素2(IL-2)及其受体拮抗剂的研究对免疫抑制药物的研制具有重要作用.抗白细胞介素2受体α链中和性单克隆抗体5G1(抗Tac型抗体)能够特异性地阻断IL-2与其受体的结合.因此,5G1可作为目标分子被用来在噬菌体展示肽库中筛选白细胞介素2的亲和性配体.经过4轮亲和性筛选以及5G1亲和活性的测定,6个具有明显5G1亲和活性的噬菌体克隆被发现.DNA序列分析结果显示出,所得到的肽序列具有明显的保守性,即SSFT(L/P)I.该序列与IL-2受体α链没有同源性.因此,SSFT(L/P)I可能模拟了IL-2受体α链上的一个不连续表位(mimotope),为白细胞介素2亲和性配体片段.     

70例颈椎病的TCD研究

目的:探讨颈椎病是否引起椎动脉(VA)的血液动力学异常。材料与方法:用TCD仪对70例颈椎病患者进行检查,指标:频谱、Vs、Vd、Vm、PI,与正常人组50人进行比较。结果:颈椎病组>40岁年龄组的指标与正常人相应年龄组差异显著(P<0.01～0.05),<40岁组无显著性差异(P>0.05),异常改变主要为单侧的VA,颈椎病组异常的VA通常有频谱的相应改变。结论:颈椎病患者TCD的改变一般为单侧的VA流速降低为主要特点。     

博尔纳病病毒抗原免疫的杂交瘤细胞系的评价

目的评价博尔纳病病毒(BDV)抗原免疫的杂交瘤细胞系的产生抗体能力与抗体特异性。方法随机抽取2株由BDV抗原免疫的杂交瘤细胞系H1和H2,通过体内和体外方法制备单克隆抗体,通过间接免疫荧光、免疫印迹和间接酶联免疫吸附3种试验方法对制备的抗体进行特异性鉴定及效价测定。结果杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。结论由杂交瘤细胞系H1和H2产生的单克隆抗体可以用于检测BDV特异性抗原。     

Antiviral Effect of Interferon-Induced Guanylate Binding Protein-1 against Coxsackie Virus and Hepatitis B Virus B3 in Vitro

Guanylate binding protein-1(GBP-1)is an interferon-induced protein.To observe its antiviral effect against Hepatitis B virus(HBV)and Coxsackie virus B3(CVB3),we constructed an eukaryotic expression vector of human GBP-1(hGBP-1).Full-length encoding sequence of hGBP-1 was amplified by long chain RT-PCR and inserted into a pCR2.1 vector,then subcloned into a pCDNA3.1(-)vector.Recombinant hGBP-1 plasmids and pHBV1.3 carrying 1.3-fold genome of HBV were contransfected into HepG2 cells,and inhibition effect of hGBP-1 against HBV replication was observed.Hela cells transfected with recombinant hGBP-1 plasmids were challenged with CVB3,and viral yield in cultures were detected.The results indicated that recombinant eukaryotic expression plasmid of hGBP-1 was constructed successfully and the hGBP-1 gene carried in this plasmid could be efficiently expressed in HepG2 cells and Hela cells.hGBP-1 inhibit CVB3 but not HBV replication in vitro.These results demonstrate that hGBP-1 mediates an antiviral effect against CVB3 but not HBV and perhaps plays an important role in the interferon-mediated antiviral response against CVB3.