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101.
全球变化和人类活动正以空前的速度在世界范围内改变着生物多样性, 这导致了全球生物多样性的锐减以及生产力的下降、病虫害的增加和抗入侵能力的减弱等生态问题。近30年来, 生态学家开始对于生物多样性的持续丧失是否以及如何影响生态系统功能的问题越来越感兴趣, 生物多样性与生态系统功能(biodiversity and ecosystem functioning, BEF)关系的研究应运而生, 并成为生态学研究的热点之一。但长期以来, 研究者更多地关注单一生态系统功能, 而忽略了生态系统能够同时提供多种生态系统功能的能力, 即生态系统多功能性(ecosystem multifunctionality, EMF)。本文综述了EMF研究中功能指标的选择、生物多样性的不同维度、微生物多样性对EMF的影响以及其他非生物因子对EMF的驱动等进展。因只考虑单一功能可能会低估生物多样性对整体生态系统功能的影响, 故生物多样性与生态系统多功能性(BEMF)关系的研究成为BEF关系研究的重点。近年来, BEMF关系的研究发展较快, 在不同生态系统(包括水生、草地、森林、旱地、农业等)、不同研究尺度(从区域到全球尺度)、BEMF关系的驱动机制(从单一驱动机制到多种驱动机制共同作用)、研究方法(包括新概念以及新的量化方法的提出和应用)等方面均取得了新的进展。但仍有不足之处, 如对于EMF研究中功能指标的选取没有统一的标准、对地下微生物多样性的关注度不够、涉及多营养级水平下的BEMF关系研究较少、驱动EMF的机制仍存在争论等。未来应加强对于功能指标选取的标准研究, 综合分析地上、地下生物多样性以及非生物因子对EMF的整体影响, 加强生态系统多服务性(ecosystem multiserviceability, EMS)方法的研究和应用。 相似文献
102.
《Biotechnic & histochemistry》2013,88(8):494-498
Argyrophilic nucleolar organizing region associated proteins (AgNORs) play roles in cell proliferation and a variety of diseases. We attempted to determine whether decreased NOR protein synthesis causes human hair loss. We studied 21 healthy males who suffered hair loss on the frontal/vertex portion of the head. Hair root cells from normal and hair loss sites were stained for AgNOR. One hundred nuclei per site were evaluated and the AgNOR number and NORa/TNa proportions of individual cells were determined using a computer program. The cells from normal sites had significantly higher AgNOR counts than those from hair loss sites. Also, the cells from the normal sites had significantly higher NORa/TNa than cells from the hair loss sites. In the normal sites, the cells demonstrated more NOR protein synthesis than cells in hair loss sites. Therefore, decreased NOR protein synthesis appears to be related to hair loss in humans. 相似文献
103.
《Biotechnic & histochemistry》2013,88(1):12-13
Ethylenediaminetetraacetic acid (EDTA) solution is used to decalcify bone specimens for histological examination. Sodium hydroxide (NaOH) has been used to dissolve EDTA and to bring EDTA solutions to neutral pH. This solution, however, requires several weeks to decalcify bone specimens. We investigated a new de-calcification fluid using concentrated ammonium hydroxide (NH4OH) to dissolve EDTA and to adjust the pH to neutral. Decalcification was performed using a magnetic stirrer with and without vacuum, or with a sonic cleaner. Decalcification end point was confirmed using both the weight loss and X-ray methods. After decalcification, specimens were processed through paraffin and sections were stained with hematoxylin and eosin. Decalcification employing NH4OH required an average of six days. Light microscopy indicated good retention of cellular detail. 相似文献
104.
Christian Schimper Rupert Keckeis Eduard Burtscher Avinash P. Manian 《Biocatalysis and Biotransformation》2013,31(5-6):383-389
Cellulosic fabric samples of cotton, viscose, lyocell and modal were pretreated with steam and dry heat in the range of 100–190°C. The samples were then treated with a Trichoderma reesei cellulase preparation (total culture filtrate – TC), with mechanical agitation, at a high enzyme dosage of 75% by weight of fabric, for 8 hours. Generally, viscose proved to be easily degradable, followed by cotton and modal. The degree of hydrolysis was the least for Lyocell. Dry heat pretreatments exerted a lower influence on degradation rate than steam pretreatments which showed a distinct maximum at a steam temperature of 130°C. The hydrolysis rate varied strongly depending on treatment conditions and fibre type. Water retention values in treated substrates were changed by up to 20% of initial values. 相似文献
105.
J. Paulo Macedo Jorge Pereira João Faria J. Luis Alves José López-López 《Computer methods in biomechanics and biomedical engineering》2013,16(12):655-662
AbstractObjectives: The purpose of the present study was to evaluate the distribution and magnitude of stresses through the bone tissue surrounding Morse taper dental implants at different positioning relative to the bone crest. Materials and Methods: A mandibular bone model was obtained from a computed tomography scan. A three-dimensional (3D) model of Morse taper implant-abutment systems placed at the bone crest (equicrestal) and 2?mm bellow the bone crest (subcrestal) were assessed by finite element analysis (FEA). FEA was carried out on axial and oblique (45°) loading at 150 N relatively to the central axis of the implant. The von Mises stresses were analysed considering magnitude and volume of affected peri-implant bone. Results: On vertical loading, maximum von Mises stresses were recorded at 6-7?MPa for trabecular bone while values ranging from 73 up to 118?MPa were recorded for cortical bone. On oblique loading at the equiquestral or subcrestal positioning, the maximum von Mises stresses ranged from 15 to 21?MPa for trabecular bone while values at 150?MPa were recorded for the cortical bone. On vertical loading, >99.9vol.% cortical bone volume was subjected to a maximum of 2?MPa while von Mises stress values at 15?MPa were recorded for trabecular bone. On oblique loading, >99.9vol.% trabecular bone volume was subjected to maximum stress values at 5?MPa, while von Mises stress values at 35?MPa were recorded for >99.4vol.% cortical bone. Conclusions: Bone volume-based stress analysis revealed that most of the bone volume (>99% by vol) was subjected to significantly lower stress values around Morse taper implants placed at equicrestal or subcrestal positioning. Such analysis is commentary to the ordinary biomechanical assessment of dental implants concerning the stress distribution through peri-implant sites. 相似文献
106.
Litter decay is a significant part of carbon budget. Due to strong environmental control, the changes in the environment may drastically influence the litter decay rates. Litter decomposition of eight dry tropical woody species, viz. Shorea robusta, Buchanania lanzan, Diospyros melanoxylon, Lagerstroemia parviflora, Lannea coromandelica, Terminalia tomentosa, Holarrhena antidysenterica and Lantana camara was studied to document the effect of intra-annual changes in the environment. Litter decomposition was monitored at monthly intervals at five sites using litter bag technique over an annual cycle in a dry tropical deciduous forest of Vindhyan highland, India. Weight loss differed among species and through months, and ranged from 15.38% in L. camara at Kotwa site in January to 30.72% in T. tomentosa at Hathinala site in August. Peak weight loss occurred in August and averaged 46.2% across species and sites. Nitrogen and phosphorus mineralization rates also varied significantly from species to species. T. tomentosa having higher nitrogen content and lower C/N ratio than other species exhibited faster weight loss. Nitrogen and phosphorus contents of litter showed significant positive correlation with weight loss. C/N ratio was negatively related to decay constant, and the weight loss was positively related to the soil surface temperature as well as soil moisture content. 相似文献
107.
108.
Karl-Frederik Bergeron Tatiana Cardinal Nicolas Pilon 《Journal of visualized experiments : JoVE》2013,(79)
Neural crest cells (NCC) are a transient and multipotent cell population that originates from the dorsal neural tube and migrates extensively throughout the developing vertebrate embryo. In addition to providing peripheral glia and neurons, NCC generate melanocytes as well as most of the cranio-facial skeleton. NCC migration and differentiation is controlled by a combination of their axial origin along the neural tube and their exposure to regionally distinct extracellular cues. Such contribution of extracellular ligands is especially evident during the formation of the enteric nervous system (ENS), a complex interconnected network of neural ganglia that locally controls (among other things) gut muscle movement and intestinal motility. Most of the ENS is derived from a small initial pool of NCC that undertake a long journey in order to colonize - in a rostral to caudal fashion - the entire length of the prospective gut. Among several signaling pathways known to influence enteric NCC colonization, GDNF/RET signaling is recognized as the most important. Indeed, spatiotemporally controlled secretion of the RET ligand GDNF by the gut mesenchyme is chiefly responsible for the attraction and guidance of RET-expressing enteric NCC to and within the embryonic gut. Here, we describe an ex vivo cell migration assay, making use of a transgenic mouse line possessing fluorescently labeled NCC, which allows precise quantification of enteric NCC migration potential in the presence of various growth factors, including GDNF. 相似文献
109.
《African Journal of Aquatic Science》2013,38(3):231-238
The Elands River, Mpumalanga, is an ecologically sensitive river that is constantly threatened by a variety of human activities. The influence of a pulp and paper mill on the river's macroinvertebrate assemblages was studied in March and June 2005, representing high- and low-flow seasons, respectively. Macroinvertebrates were collected from various biotopes using a standard sampling net, and were preserved and identified to family level. A range of multivariate and univariate statistical analyses was applied to assess the spatial and temporal variations in the macroinvertebrate communities. Diversity loss and dominance by a single taxon, Melanoides tuberculata, were noted directly below the mill. Other sites did not appear to be affected. Macroinvertebrate communities at all sites showed a strong seasonal variation. 相似文献
110.