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141.
A weathered medium crude oil was applied to experimental plots of Scirpus pungens (Three-square Bulrush) in a freshwater wetland to determine the efficacy of strategies for shoreline oil spill bioremediation based on nutrient enrichment (bioremediation) and plant growth (phytoremediation). Plots were unoiled, oiled with no added nutrients, or oiled with repeated applications of phosphate and nitrate fertilizers. Following initial treatments, the experimental plots were raked to simulate the activity of wave action on oil penetration, and plants in one fertilized plot were cut repeatedly. The sediments were sampled at regular intervals for 15 months after oiling, and the loss of oil was assessed by 4-day laboratory tests of polynuclear aromatic hydrocarbon (PAH) bioaccumulation by trout, as demonstrated by increases in activity of liver cytochrome P450 (CYP1A) enzymes. Oil alone, oil mixed with sediments in the lab, and oiled sediments from treated plots all induced CYP1A activity relative to untreated controls, indicating the presence and bioavailability of PAH. Induction did not vary with nutrient treatments, but declined by 80% within 15 months of oiling, and chemical analyses indicated equivalent losses of hydrocarbons in sediment. These results demonstrate that bioavailable PAHs persisted in measurable quantities for at least 1.25 years following oiling, and that stimulation of plant growth did not affect the rate of oil disappearance. The controlling factors were likely weathering and sediment movement. 相似文献
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Paul W. Weber Laurens E. Howle Mark M. Murray Joseph M. Corless 《Biophysical journal》2011,100(3):525
When radiolabeled precursors and autoradiography are used to investigate turnover of protein components in photoreceptive cone outer segments (COSs), the labeled components—primarily visual pigment molecules (opsins)—are diffusely distributed along the COS. To further assess this COS labeling pattern, we derive a simplified mass-transfer model for quantifying the contributions of advective and diffusive mechanisms to the distribution of opsins within COSs of the frog retina. Two opsin-containing regions of the COS are evaluated: the core axial array of disks and the plasmalemma. Numerical solutions of the mass-transfer model indicate three distinct stages of system evolution. In the first stage, plasmalemma diffusion is dominant. In the second stage, the plasmalemma density reaches a metastable state and transfer between the plasmalemma and disk region occurs, which is followed by an increase in density that is qualitatively similar for both regions. The final stage consists of both regions slowly evolving to the steady-state solution. Our results indicate that autoradiographic and cognate approaches for tracking labeled opsins in the COS cannot be effective methodologies for assessing new disk formation at the base of the COS. 相似文献
145.
截线抽样法广泛应用于鲸豚类动物的考察。通过文献调查并结合我们自己的研究工作,本文从考察设计、
野外考察和数据分析三个方面分析了截线抽样法在我国水域应用时可能出现的问题。针对考察设计,提出了截
线设计的建议。针对野外考察,主要从观察平台、人员配置、观察方法和记录方法几个方面提出建议。针对分
析过程,介绍了使用R 编程平台和Distance 软件进行数据预处理和数据分析的简要过程。笔者期望本文的相关
建议有助于促进我国鲸豚类考察方法的规范化。 相似文献
野外考察和数据分析三个方面分析了截线抽样法在我国水域应用时可能出现的问题。针对考察设计,提出了截
线设计的建议。针对野外考察,主要从观察平台、人员配置、观察方法和记录方法几个方面提出建议。针对分
析过程,介绍了使用R 编程平台和Distance 软件进行数据预处理和数据分析的简要过程。笔者期望本文的相关
建议有助于促进我国鲸豚类考察方法的规范化。 相似文献
146.
The Bacterial flagellar filament can undergo a polymorphic phase transition in response to both mechanical and chemical variations in vitro and in vivo environments. Under mechanical stimuli, such as viscous flow or forces induced by motor rotation, the filament changes its phase from left-handed normal (N) to right-handed semi-coiled (SC) via phase nucleation and growth. Our detailed mechanical analysis of existing experiments shows that both torque and bending moment contribute to the filament phase transition. In this paper, we establish a non-convex and non-local continuum model based on the Ginzburg-Landau theory to describe main characteristics of the filament phase transition such as new-phase nucleation, growth, propagation and the merging of neighboring interfaces. The finite element method (FEM) is adopted to simulate the phase transition under a displacement-controlled loading condition (rotation angle and bending deflection). We show that new-phase nucleation corresponds to the maximum torque and bending moment at the stuck end of the filament. The hysteresis loop in the loading and unloading curves indicates energy dissipation. When the new phase grows and propagates, torque and bending moment remain static. We also find that there is a drop in load when the two interfaces merge, indicating a concomitant reduction in the interfacial energy. Finally, the interface thickness is governed by the coefficients of the gradient of order parameters in the non-local interface energy. Our continuum theory and the finite element method provide a method to study the mechanical behavior of such biomaterials. 相似文献
147.
沼液的定价方法及其应用效果研究 总被引:4,自引:0,他引:4
为了探索沼液的商品化,采用养分含量定价和应用效果定价2种方法确定了沼液的价格,并分析了沼液施用后对土壤基本理化性状的影响作用。结果发现:采用养分含量定价法得到的沼液价格为78.12元/m3,采用沼液应用效果定价法得到的沼液的价格为111.4元/ m3;沼液的实际应用价值要明显高于其养分含量的市场价;沼液施用后可降低土壤容重,增加土壤孔隙度,增加土壤有机质、土壤全氮、土壤有效磷及有效钾含量,有利于土壤肥力的保持。 相似文献
148.
149.
Hong-Xin Fei Ting Liu Xiao-Jie Zhang Shu-Liang Wu 《Bioscience, biotechnology, and biochemistry》2018,82(1):57-64
Alzheimer’s disease (AD) is the most common cause of dementia among elderly population. Deranged β-amyloid (Aβ) trafficking across the blood–brain barrier is known to be a critical element in the pathogenesis of AD. In the vascular endothelial cells of hippocampus, Aβ transport is mainly mediated by low-density lipoprotein-associated protein 1 (LRP1) and the receptor for advanced glycation end (RAGE) products; therefore, LRP1 and RAGE endothelial cells are potential therapeutic targets for AD. In this study, we explored the effects of Formononetin (FMN) on learning and memory improvement in APP/PS1 mice and the related mechanisms. We found that FMN significantly improved learning and memory ability by suppressing Aβ production from APP processing, RAGE-dependent inflammatory signaling and promoted LRP1-dependent cerebral Aβ clearance pathway. Moreover, FMN treatment alleviated ultrastructural changes in hippocampal vascular endothelial cells. In conclusion, we believe that FMN may be an efficacious and promising treatment for AD. 相似文献
150.
M. Pietsch F. Niemeyer U. Simon A. Ignatius K. Urban 《Computer methods in biomechanics and biomedical engineering》2018,21(8):512-520
We present a novel numerical model of the fracture-healing process using interface-capturing techniques, a well-known approach from fields like fluid dynamics, to describe tissue growth. One advantage of this method is its direct connection to experimentally observable parameters, including tissue-growth velocities. In our model, osteogenesis, chondrogenesis and revascularisation are triggered by mechanical stimuli via mechano-transduction based on previously established hypothesis of Claes and Heigele. After experimentally verifying the convergence of the numerical method, we compare the predictions of our model with those of the already established Ulm bone-healing model, which serves as a benchmark, and corroborate our results with existing animal experiments. We demonstrate that the new model can predict the history of the interfragmentary movement and forecast a tissue evolution that appears similar to the experimental results. Furthermore, we compare the relative tissue concentration in the healing domain with outcomes of animal experiments. Finally, we discuss the possible application of the model to new fields, where numerical simulations could also prove beneficial. 相似文献