EUS图像胰腺癌分类中粒子光斑的去除

已有基于图像纹理特征对胰腺超声内镜图像进行是否为癌症的分类方法.放射性粒子植入治疗是一种较常用的姑息治疗方法.治疗后的图像中由植入粒子所产生的光斑对分类的结果有一定的影响.通过使用改进的中值滤波方法去除光斑后分类结果的准确性有了一定的提高.     

Smear morphology of cryptococcosis presenting as a subcutaneous swelling in healthy adults: a report of three cases

OBJECTIVE: To highlight the various morphological smear pattern in cases of subcutaneous Cryptococcus infection in healthy adults. METHOD: Cryptococcus is an opportunist fungus and primary infection is acquired through respiratory tract. Dissemination by blood stream results in systemic infection. Ten to 15% of systemic infection present as cutaneous lesions. Between December 2002 and April 2004 three healthy adults presented to us consecutively with subcutaneous swelling. RESULTS: We diagnosed these cases on FNAC as Cryptococcus. In all the three patients there was no history of local penetrating injury and any signs or symptoms of systemic disease. They were two male and one female, immuno competent and were negative for HIV 1 & 2 tested by ELISA. The sites were right abdominal flank, occipital and left anterior upper thigh. Aspirated materials were oily fibro fatty tissue and necrotic purulent materials. Cryptococcus numbers varied in all the smears so also their size and capsule thickness. Background smear morphology and tissue reaction were also different. It could be gelatinous, granulomatous, and cellulitic response or mixed responses and this can be picked up on cytological smears. Cultures were confirmatory in all the three cases. These lesions were resolved with antifungal treatment. CONCLUSION: Our brief article highlights the morphological spectrum on FNAC smears and diagnostic problems faced in these uncommon circumstances where the aspirates were purulent and the yeasts were small, few and thin walled. In the acute inflammatory smear with occasional giant cells and/or granulomas special stains like PAS or Mucicarmine are necessary to look for budding yeast of Cryptococcus with thin neck.     

Aphanomyces invadans,the causal agent of Epizootic Ulcerative Syndrome,is a global threat to wild and farmed fish

Aphanomyces invadans is a eukaryotic pathogen and the causative agent of Epizootic Ulcerative Syndrome (EUS) in fish and is responsible for mortalities of up to 100% in aquaculture. A. invadans was first discovered in Japan in 1971, and since then it has been found in Australia, North America, Southern African countries and Asia. Methods for the correct identification of A. invadans are well established now and involve PCR-based detection and microscopy. However, the pathogenesis of A. invadans is poorly understood. Environmental stress (mainly temperature) and the associated immunocompromised fish seem to induce infections of A. invadans and outbreaks of EUS. Understanding the process of infection in more depth is fundamental for the discovery of novel effective treatments to combat the disease. In this review, we discuss morphological characteristics of A. invadans and its pathogenicity as well as various approaches of treatment.