错位双链RNA,丙氧鸟苷和原核细胞DNA促旋酶B对鸭乙肝病毒的抑制作用

人工合成错位双链ＲＮＡ（ａｍｐｌｉｇｅｎ）是一种已知的干扰素诱导剂。单独和／或与其它抗病毒药物联合使用治疗鸭乙型肝炎病毒（ＤＨＢＶ）阳性鸭。这些药物包括丙氧鸟苷（ｇａｎｃｉｃｌｏｖｉｒ）和原核细胞ＤＮＡ促旋酶Ｂ抑制物（ｃｏｕｍｅｒｍｙｃｉｎＡｌ）。单独和／或联合该两种药物时,对鸭血清和肝组织内ＤＨＢＶ均有抑制作用,但以三种药物联合时抑制效果最好。上述下种方案治疗结束后肝内松弛环状ＤＮＡ（ＲＣ）和超螺旋ＤＮＡ（ＳＣ）持续存在,而且不能消除血中鸭乙肝表面抗原（ＤＨＢｓＡｇ）。对治疗的大多数鸭血清干扰素,测定结果表明,ａｍｐｌｉｇｅｎ能诱生鸭干扰素,但其出现时间与抗病毒效果产生没有平行关系。本研究为人慢性乙型肝炎治疗合理使用抗病毒药物和免疫诱导剂提供了参考。     

[卡其].肯拜尔鸭和北京鸭胰脏和胰管的比较解剖

本研究共观察[卡其]·肯拜尔鸭(Khaki Campbell)45只,北京鸭[Pekin duck(Anas domestica L.)]41只,比较了两种鸭胰叶和胰管的形态结构。结果如下:[卡其]·肯拜尔鸭和北京鸭的胰脏都分为背侧胰叶,腹侧胰叶和脾胰叶。除有背侧胰管和腹侧胰管外,还观察到自背侧胰叶尾端发出另一胰管入十二指肠,并命名为第一胰管。[卡其]·肯拜尔鸭和北京鸭的背侧和腹侧胰管入肠类型均可分为三种:背、腹侧胰管以左、右向排列;背侧胰管在前,腹侧胰管在后;腹侧胰管在前,背侧胰管在后。此外,[卡其]·肯拜尔鸭出现多条胰管入肠的例数多于北京鸭。     

安徽庐江鸭乙型肝炎病毒LJ-76转染细胞系的建立

为建立鸭乙型肝炎病毒ＬＪ－７６的转染细胞系,将ＬＪ－７６病毒ＤＮＡ插入到ｐＵＣ１９的ＥｃｏＲⅠ位点上,分离得到含有双拷贝ＬＪ－７６ＤＮＡ的重组质粒．通过磷酸钙沉淀方法,将经ＣｓＣｌ等密度离心纯化的ＬＪ－７６ＤＮＡ双体导入到人肝癌细胞ＢＥＬ７４０２中．收集转染细胞的培养液进行蔗糖密度梯度离心,所得沉淀经检测发现含有ＬＪ－７６ＤＮＡ并具有特异性ＤＨＢＶ内源性ＤＮＡ多聚酶活性;对上述样品通过ＤｏｔＥＩＡ检测ＤＨＢＶ核心抗原及表面抗原结果为阳性．Ｓｏｕｔｈｅｒｎｂｌｏｔ分析表明转染细胞内存在病毒ＤＮＡ复制中间体ｃｃｃＤＮＡ、ｓｓＤＮＡ和ｒｃＤＮＡ,而ｃｃｃＤＮＡ被认为是复制活动较为活跃的标志．电镜观察转染细胞的上清发现有病毒颗粒的存在．     

用PCR检测临床血标本中人类细小病毒B19 DNA

用ＰＣＲ检测临床血标本中人类细小病毒Ｂ１９　ＤＮＡ杨洪江,张桦,林书祥,牛艾茹（天津市儿童医院儿研所,天津３０００７４）关键词聚合酶链反应（ＰＣＲ）,人类细小病毒Ｂ１９几种人类细小病毒中,只有Ｂ１９病毒是人类的病原体。由于该病毒不能在体外繁殖,获得该...     

Changes at the ecto-mesodermal interface during development of the duck preen gland

Summary An ultrastructural investigation of the organogenesis of the duck preen gland showed variations at the ecto-mesodermal interface in the course of development. During the period of invagination, ectoderm and mesoderm were separated by a continuous basal lamina. Morphogenesis of the tubules is characterized by a preferential deposition of non-oriented collagen fibres localized at the branching sites. Direct contacts between ectodermal extensions and mesodermal cells, through gaps in the basal lamina, appeared at the end-buds after the morphogenetic pattern was established and before the onset of the glandular secretory activity. The correlation between the modification of the ecto-mesodermal interface and the differentiation of uropygial ectoderm is discussed.     

Mechanistic failure mode investigation and resolution of parvovirus retentive filters

Virus retentive filters are a key product safety measure for biopharmaceuticals. A simplistic perception is that they function solely based on a size‐based particle removal mechanism of mechanical sieving and retention of particles based on their hydrodynamic size. Recent observations have revealed a more nuanced picture, indicating that changes in viral particle retention can result from process pressure and/or flow interruptions. In this study, a mechanistic investigation was performed to help identify a potential mechanism leading to the reported reduced particle retention in small virus filters. Permeate flow rate or permeate driving force were varied and analyzed for their impact on particle retention in three commercially available small virus retentive filters. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:959–970, 2016     

猪细小病毒VP2蛋白主要抗原域间接ELISA方法的建立及初步应用

将已获得的含猪细小病毒VP2蛋白主要抗原域编码基因VP2I重组酵母菌株在优化的条件下进行诱导表达,表达产物蛋白含量达227.6μg/mL,在此基础上以重组蛋白作为包被抗原初步建立了检测猪细小病毒抗体水平的间接ELISA方法,并对该方法进行了优化,结果表明抗原最佳包被浓度5.69μg/mL,而血清最佳稀释倍数为1:80。阳性标准初步定为：OD待测样品＞0.5,且OD待测样品/OD阴性血清＞2.0。采用iVP2I-ELISA对猪血清样品进行检测,结果显示iVP2I-ELISA与HI试验的符合率为97.2%,与国外同类试剂盒的符合率达到91.2%。     

一氧化氮对猪细小病毒体外增殖的影响研究

本文研究了来源于不同前体物的一氧化氮(Nitro oxide,NO)对猪细小病毒(Porcine parvovirus,PPV)体外增殖的影响.结果表明,NO前体物S-硝基-N-乙酰青霉胺(SNAP)、L-精氨酸(L-Arg)均能够有效地诱导PK-15细胞产生NO,进而显著地抑制PPV在PK-15细胞上的复制,其效果与前体物的浓度呈正相关,在浓度为100μmol/L和200μmol/L时,SNAP产生NO的能力与抑制病毒复制的作用要强于L-Arg.在病毒感染前6 h和3 h添加SNAP或L-Arg对病毒复制的抑制作用比在病毒感染后3 h和6 h添加的作用强,表明NO的抗病毒作用主要发生在病毒感染的初始阶段.此外,添加具有抑制L-Arg产生NO作用的N-硝基-L-精氨酸(L-NNA)能抵消L-Arg体外抗病毒的作用.