Continuous counter‐current chromatography for capture and polishing steps in biopharmaceutical production

The economic advantages of continuous processing of biopharmaceuticals, which include smaller equipment and faster, efficient processes, have increased interest in this technology over the past decade. Continuous processes can also improve quality assurance and enable greater controllability, consistent with the quality initiatives of the FDA. Here, we discuss different continuous multi‐column chromatography processes. Differences in the capture and polishing steps result in two different types of continuous processes that employ counter‐current column movement. Continuous‐capture processes are associated with increased productivity per cycle and decreased buffer consumption, whereas the typical purity‐yield trade‐off of classical batch chromatography can be surmounted by continuous processes for polishing applications. In the context of continuous manufacturing, different but complementary chromatographic columns or devices are typically combined to improve overall process performance and avoid unnecessary product storage. In the following, these various processes, their performances compared with batch processing and resulting product quality are discussed based on a review of the literature. Based on various examples of applications, primarily monoclonal antibody production processes, conclusions are drawn about the future of these continuous‐manufacturing technologies.     

Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors

Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g_{Laminaribiose} g_sugar^?1 in the desorbate with a total productivity of 5.6 mg_{Laminaribiose} L_{enzyme bed}^?1 h^?1 without the use of recycles.     

Seed floating ability and distribution of alpine plants along a northern Swedish river

Abstract. In this study we tested the hypothesis that diaspore buoyancy is an important factor in determining the distribution range of riparian plants, by measuring the floating capacity of fruits and seeds from 17 alpine vascular plant species during 1 yr. Minimum, median, mean, and maximum (≤ 1 yr) floating times of fruits and seeds were then related to the downstream distribution of plants along the free-flowing Vindel River in northern Sweden. The distances from the alpine headwater region to the most downstream occurrence of plants, and to the cessation of a more continuous distribution, were evaluated. The variation in downstream limits of plants could not be statistically explained by variation in seed buoyancy between species, indicating that floating ability of seeds is not important for plant distribution patterns. There may be several reasons for this, and they may differ between species.     

Environmental impacts of forest production and supply of pulpwood: Spanish and Swedish case studies

Background, aim and scope  Forest operations use large amounts of energy, which must be considered when life cycle assessment (LCA) methodology is applied to forest products. Forest management practices differ considerably between countries and may also differ within a country. This paper aims to identify and compare the environmental burdens from forest operations in Sweden and Spain focused on pulpwood production and supply to pulp mills. Materials and methods  To perform the analysis, the main forest plantations were investigated as well as the most important tree species used in pulp mills in both countries: eucalyptus and, Norway spruce and Scots pine, were taken into account for the Spanish and Swedish case studies, respectively. Energy requirements for pulpwood production and supply to Spanish and Swedish pulp mills are evaluated in this paper. All forest operations from site preparation to extraction of felled wood to the delivery point at the roadside are included within the system boundaries as well as wood transport from forest landing to the pulp mill gate. Seedling and machinery production are excluded from the system boundaries due to lack of field data. The impact assessment phase was carried out according to the Swedish Environmental Management Council and, in particular, the impact categories assessed in forest and agricultural LCAs (global warming, acidification, eutrophication and photochemical oxidant formation) were analysed. SimaPro 7.10 software was used to perform the impact assessment stage. Results  Different types of wood are produced in both case studies: softwood in Sweden and hardwood in Spain, with higher production of round wood and biomass per hectare in Sweden. Total energy use for pulpwood production and supply are in a similar order of magnitude, up to 395 MJ and 370 MJ/m³ solid under bark in Spain and Sweden, respectively. Field operations, such as silviculture and logging, are more energy-intensive in the Spanish case study. However, secondary hauling of pulpwood to pulp mill requires more energy in the Swedish case study. These important differences are related to different forest management practices as well as to pulpwood supply to the pulp mill. The eventual imports of pulpwood, application of pesticides, thinning step or final felling considerably affects energy requirements, which are reflected on the environmental results. Discussion  Although differences between both case studies were observed, several stages were investigated: wood delivery to the pulp mill by road, harvesting and forwarding, contribute considerably to acidification, eutrophication and global warming potential in both cases. The type of wood, the machines used in forest operations (mechanised or motor-manual), the use of fossil fuels and the amount of wood produced influence the results. These differences must be kept in mind in comparative studies between such different countries. Conclusions  The results obtained in this work allow one to forecast the importance of forest operations in LCA of forest products (in this case, wood pulp) and the influence of energy use in the results. Special attention has been paid in the inventory analysis stage for both case studies. It is possible to gain a better environmental performance in both case studies if alternative practices are considered, mainly focused on site preparation and stand tending in the Spanish system and on pulpwood supply in the Swedish one. Recommendations and perspectives  This study provides useful information that can assist forest-based industries in the aim of increasing their sustainability. Future work will focus on the study of several transport alternatives of pulpwood supply including railway, road and ship. In addition, pulpwood processing in Spanish and Swedish paper pulp mills considered to be representative of the “state of art” will be carried out in order to get a complete picture of this kind of forest-based industry. In addition, the use of biofuels (such as forest biomass) instead of fossil fuels and CO₂ uptake of wood via photosynthesis will be carried out in order to have a complete perspective of forest ecosystems.     

Anatomy of deception: A behavioral contingency analysis

Deception, a basic and pervasive biological phenomenon, takes many forms, variously referred to as mimicry, trickery, seduction, pretense, feigning, masquerading, impersonation, distraction, or false promises, and these share certain common distinguishing behavioral elements that permit them to be classified into categories. A symbolic language for the codification and analysis of behavioral contingencies shows that all instances of deception are based on a misperception, misprediction, non-perception, or non-prediction by the deceived party, and can be further categorized based on features of the contingencies that define them. Instances of particular interest are those in which a deceiving party predicts (and in that sense “intends”) the deception. In those instances, the effect of the deception is usually to the deceiving party's benefit and to the deceived party's detriment.In economics, finance, business, military operations, public affairs, education, and everyday social interaction, deception takes numerous forms. Special forms, usually involving obfuscation, concealment, counterfeiting, and misrepresentation, occur in certain prevalent types of property transfer, including securitization, the creation of derivatives, and various types of Ponzi schemes. Such property transfers tend to be driven by opportunities for deception. They all involve blurring and clouding of the contingencies that defined the transferred properties, thus permitting their obfuscation.     

Logical Structures of Young Chimpanzees' Spontaneous Object Grouping

I investigated sensorimotor precursors of logicoarithmetic operations involved in spontaneous object grouping of five common chimpanzees aged 1 to 4 years. I considered three basic logical relations between objects or sets of objects: equivalence, order, and reversibility relations. Chimpanzees introduced equivalence and order relations within sets of objects as well as between sets. They showed a higher level of logical organization than monkeys do. Compared to human infants, chimpanzees manifested a specific difference and a specific delay. First, chimpanzees only partially mapped the logical properties of their action organization on sets of objects. For example, chimpanzees' simultaneous acts on two sets of objects, though increasing with age, almost never resulted in spatial correspondences between the sets. Secondly, they showed a specific delay in that they produced scarcely any form of reversibility.     

石器研究中“操作链”的概念、内涵及应用

作为旧石器研究中十分重要的研究概念之一, 本文对"操作链"的发展史、理论内涵、实践方法等方面进行讨论, 认为"操作链"概念是一种动态的、综合的理论视角和研究体系, 强调了石器技术系统的两个行为过程(技术表现与思维运作)和一个互动关系(操作序列)。实践应用和术语对比, 为更好地运用"操作链"研究石器并复原史前技术体系提供了参考。文章还提出了"操作链"概念本身存在的问题, 希望能在今后的工作中得到完善。     

福建漳州青花菜栽培钾肥施用技术优选

以青花菜品种‘炎秀’Brassica oleracea var．italica ‘yanxiu’为材料,通过设置钾肥横向与纵向施肥的田间试验,探讨青花菜最佳钾肥用量和最佳钾肥运筹。结果表明,在设定氮、磷肥施用量分别为30.0 kg/667.7 m2、10.0 kg/667.7 m2的条件下,钾肥不同施用量对青花菜的株高、苗期功能叶片数、植株扩展度及全株重（去根）、单球重均有一定的影响,当钾肥施用量为19.1 kg/667.7 m2时,其产量最高,为1763.5 kg/hm2;在同样氮、磷肥的背景条件下,钾肥施用量以15.0 kg/667.7 m2,按基肥/追肥不同比例进行施用,青花菜所表现的株高、苗期功能叶片数、植株扩展度及全株重、单球重等形态及产量指标均有所差异,当基肥∶追肥按0.2∶0.8进行施用时,该产量指标均明显高于其它配比的处理。     

The effects of a split sleep–wake schedule on neurobehavioural performance and predictions of performance under conditions of forced desynchrony

Extended wakefulness, sleep loss, and circadian misalignment are factors associated with an increased accident risk in shiftwork. Splitting shifts into multiple shorter periods per day may mitigate these risks by alleviating prior wake. However, the effect of splitting the sleep–wake schedule on the homeostatic and circadian contributions to neurobehavioural performance and subjective assessments of one’s ability to perform are not known. Twenty-nine male participants lived in a time isolation laboratory for 13?d, assigned to one of two 28-h forced desynchrony (FD) schedules. Depending on the assigned schedule, participants were provided the same total time in bed (TIB) each FD cycle, either consolidated into a single period (9.33?h TIB) or split into two equal halves (2?×?4.67?h TIB). Neurobehavioural performance was regularly assessed with a psychomotor vigilance task (PVT) and subjectively-assessed ability was measured with a prediction of performance on a visual analogue scale. Polysomnography was used to assess sleep, and core body temperature was recorded to assess circadian phase. On average, participants obtained the same amount of sleep in both schedules, but those in the split schedule obtained more slow wave sleep (SWS) on FD days. Mixed-effects ANOVAs indicated no overall difference between the standard and split schedules in neurobehavioural performance or predictions of performance. Main effects of circadian phase and prior wake were present for both schedules, such that performance and subjective ratings of ability were best around the circadian acrophase, worst around the nadir, and declined with increasing prior wake. There was a schedule by circadian phase interaction for all neurobehavioural performance metrics such that performance was better in the split schedule than the standard schedule around the nadir. There was no such interaction for predictions of performance. Performance during the standard schedule was significantly better than the split schedule at 2?h of prior wake, but declined at a steeper rate such that the schedules converged by 4.5–7?h of prior wake. Overall, the results indicate that when the total opportunity for sleep per day is satisfactory, a split sleep–wake schedule is not detrimental to sleep or performance. Indeed, though not reflected in subjective assessments of performance capacity, splitting the schedule may be of some benefit, given its reduction of neurobehavioural impairment at night and its association with increased SWS. Therefore, for some industries that require operations to be sustained around the clock, implementing a split work–rest schedule may be of assistance.