污网蝽属记述及一新种描述(半翅目,网蝽科)

补充头刺、后胸臭腺和生殖节等特征后重新定义了东洋区特有属-污网蝽属,并确认该属单质.记述中国污网蝽属昆虫3种,其中狄氏污网蝽Ildefonsus distanti为新种,窄污网蝽Ildefonsus nexus Drake et Ruhoff为中国新纪录种.文中给出了污网蝽属名录及种类检索表.新种的模式标本保存于南开大学昆虫标本室.     

Genomic DNA microarrays for Entamoeba histolytica: applications for use in expression profiling and strain genotyping

The parasite Entamoeba histolytica is a causative agent of dysentery and liver abscesses. Found predominantly in developing countries, this parasitic infection is responsible for significant morbidity and mortality. We have developed a genomic DNA microarray for E. histolytica. The array composed of 11,328 clones contains >2000 unique genes and was utilized for expression profiling and comparative genomic hybridizations of Entamoeba strains. We present a synopsis of our results to date and potential future applications of microarray technology for the study of Entamoeba biology.     

主要作物远缘杂交概况

本文综述了水稻、小麦、玉米、棉花、大豆等作物的远缘杂交的概况。这五种作物远缘杂交中,利用的野生近缘植物有水稻的尼瓦拉野生稻、普通野生稻和药用野生稻,小麦的黑麦、偃麦草、冰草、山羊草、簇毛麦、赖草、鹅观草、新麦草、旱麦草,玉米的类玉米和摩擦禾,棉花的墨西哥野生棉、异常棉、瑟伯氏棉、李奇蒙德氏棉、司笃克氏棉和比克氏棉,大豆的野生大豆和半野生大豆。通过远缘杂交获得了新物种如小偃麦、亚比棉等,还获得了大批异附加系、异代换系、易位系等种质材料,以及生产上推广的优良品种。     

利用普通小麦与近缘属间的复合杂交创造小麦新种质

利用永368(普通小麦)与春品-11(白壳、六倍体小黑麦) 、人工合成小麦双二倍体、柱穗山羊草进行复合杂交,采用系谱法,按照宁夏小麦品种选育目标,经过连续十年的选择,培育出10个稳定优良品系,分别为春节1、2、3、4、5、6号和春山1、2、3、4号.它们与宁夏目前生产上的主栽品种宁春4号所属的遗传类群不同,品质也有明显改进.染色体组原位杂交和醇溶蛋白电泳鉴定结果表明,新创造的7个选系是黑麦染色体1B/1R易位系,另外一个品系是涉及黑麦其他染色体的易位系.     

红菜薹与甘蓝型油菜杂交子房培养研究初报

对甘蓝型油菜和红菜薹种间杂种进行胚胎挽救研究,实验结果表明,采用MS＋（1．0～2．0）mg·L^-1 6-BA＋0．05mg·L^-1 NAA＋0．5％活性炭＋30g·L^-1蔗糖＋7．5g·L^-1琼脂培养基对甘蓝型油菜和红菜薹杂交子房培养效果较为理想;相对于培养基和激素,活性炭对子房培养的影响更加显著。通过对取材时间的研究发现,取授粉后18d子房培养的结籽率最高,15d的次之。而通过对杂种萌发率的研究则表明,授粉后15d的子房培养获得的杂种萌发率最高,为57．03％,18d的最差,仅为38．49％。     

Haemolysin genes of Vibrio cholerae: presence of homologous DNA in non-haemolytic O1 and haemolytic non-O1 strains

Abstract The structural gene for the haemolysin and two accessory genes from a Vibrio cholerae O1 El Tor strain have previously been cloned in Escherichia coli K-12 to give the plasmid pPM431. This plasmid has been used as a probe with a variety of O1 and non-O1 Vibrio cholerae strains to examine by Southern DNA hybridisations for the presence of homologous DNA. Such experiments show that the DNA homologous to that present in pPM431 is present in all of the 20 strains examined, whether they were haemolytic or non-haemolytic, implying that the genes were present but not expressed in non-haemolytic strains. Using a variety of restriction enzymes to cut the chromosomal DNA of different V. cholerae strains and probing with pPM431, it was possible to distinguish O1 and non-O1 strains, as well as haemolytic or non-haemolytic strains. This variability between hly⁺ and hly⁻ may be indicative of a change in the regulatory region of the haemolysin genes. The results also imply a high degree of homology of the haemolysin of O1 and non-O1 strains.