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51.
WenTing Lu ShaoJun Liu Yu Long Min Tao Chun Zhang Jing Wang Jun Xiao Song Chen JinHui Liu Yun Liu 《Cell and tissue research》2009,336(1):159-163
We have undertaken comparative studies of the number and phenotypes of erythrocytes in the peripheral blood of red crucian
carp (RCC), blunt snout bream (BSB), and their hybrids, including triploids, tetraploids, and pentaploids. The results indicate
that the mean nuclear volume of erythrocytes in peripheral blood increases regularly with increasing ploidy. Furthermore,
many more mature erythrocytes have a dumbbell nucleus in the peripheral blood of polyploid hybrids compared with their diploid
parents. With the increase in ploidy level, the percentage of such erythrocytes increases. The polyploid hybrids also have
a large number of erythrocytes with abnormal shapes. For example, round and tear-shaped erythrocytes have been observed in
the peripheral blood of polyploid hybrids. Since the erythrocytes in polyploid hybrids with their larger volume and lower
specific surface area are unfavorable for the conveyance of oxygen, morphological variations of erythrocytes might improve
defective blood circulation.
This research was supported by the National Natural Science Fund for Distinguished Young Scholars (grant no. 30725028), the
National Natural Science Foundation of China (grant nos. 30330480 and 30571444), National Basic Research Program (grant no.
2007CB109206), and Key Project of Hunan Province (grant no. 2006NK2008). 相似文献
52.
Daniel J. Rigden 《FEBS letters》2009,583(10):1555-814
The Cry and Cyt classes of insecticidal toxins derived from the sporulating bacterium Bacillus thuringiensis are valuable substitutes for synthetic pesticides in agricultural contexts. Crystal structures and many biochemical data have provided insights into their molecular mechanisms, generally thought to involve oligomerization and pore formation, but have not localised the site on Cyt toxins responsible for selective binding of phospholipids containing unsaturated fatty acids. Here, distant homology between the structure of Cyt toxins and Erwinia virulence factor (Evf) is demonstrated which, along with sequence conservation analysis, allows a putative lipid binding site to be localised in the toxins. 相似文献
53.
54.
55.
福建大叶蝉科一新记录属及三新种(同翅目:叶蝉总科) 总被引:2,自引:0,他引:2
本文记述大叶蝉科1中国新记录属──暗大叶蝉属SeasogoniaYoung与1新种──红暗大叶蝉S.sanguineaKuohetzhuo,以及条大叶蝉属AtkinsoniellaDistant2新种──黑背条大叶蝉A.nigridorsumKuohetzhuo与红脉条大叶蝉A.rubrivenosaKuohetZhuo。均采自福建。 相似文献
56.
鲢的远缘杂交子代和人工三倍体的同工酶表达 总被引:11,自引:1,他引:10
用聚丙烯酰胺梯度凝胶电泳分析了鲢♀×鳙♂、鲢♀×团头鲂♂的二倍体和异源三倍体杂种,以及同源三倍体鲢及其亲本种的8种蛋白质和同工酶的表型。二倍体杂交子代的基因表达是多样的,这些多样的表达可能与双亲的这些蛋白质和同工酶的表型差异有关。如与二倍体杂交子代相比,鲢×鳙异源三倍体的肌肉蛋白和血清蛋白有效一致的表型,此外在两种异源三倍体中EST和MDH同工酶的表型接近母本,这些结果表明异源三倍体由于增加了一套 相似文献
57.
Gert Ooms Tonny J. G. Regensburg-Tuink Marten H. Hofker Andre Hoekema Paul J. J. Hooykaas Rob A. Schilperoort 《Plant molecular biology》1982,1(4):265-276
Stable cointegrates between incRh-1 octopine (Ach5) and nopaline (C58) Ti-plasmids, present in ten independently isolated Agrobacterium tumefaciens strains, showed identical restriction endonuclease patterns. Each cointegration event had taken place in the common sequence between the T-regions of both Ti-plasmids. This illustrates a high preference for this region when used in the formation of cointegrates. Four crown gall tissues, obtained after transformation of Nicotiana tabacum cells by one of the mutants, were analysed by using Southern blot analysis for their T-DNA structure. The borders of T-DNA frequently appeared to differ from T-DNA borders previously detected in tumour tissues that had been induced by Agrobacterium strain C58 or Ach5. Therefore, it was concluded that possibly a less stringent mechanism exists for the integration into plant DNA of T-DNA, derived from a composite (octopine/nopaline) T-region than for integration of T-DNA from a normal (octopine or nopaline) T-region.Abbreviations Agr
sensitivity to agrocin 84
- Ape
phage Apl exclusion
- Cb
resistance to carbenicillin
- Occ
octopine catabolism
- Ocs
octopine synthesis
- Noc
nopaline catabolism
- Nos
nopaline synthesis
- Rec
recombination
- Tra
transfer
- Vir
virulence 相似文献
58.
Susan Wilkie Gorman Robert Dixon Teasdale Christopher Ashley Cullis 《Plant Systematics and Evolution》1992,183(3-4):223-234
A 5S rRNA gene (5S DNA) from the coniferPinus radiata
D. Don has been cloned and characterized at the nucleotide, genomic and chromosomal levels. Sequencing revealed a repeat unit of 524 base pairs which is present in approximately 3000 copies per diploid genome. Two-dimensional gel electrophoresis indicated that these copies are organized in tandem arrays of various length. Using in situ hybridization techniques, the tandem arrays appear to be present on all of the chromosomes. This complexity of chromosomal organization contrasts markedly with the few sites of uniform length found in angiosperm plants such as wheat, pea, and maize. 相似文献
59.
Anaerobic sludge granules were obtained from laboratory-scale anaerobic bioreactors used to treat pharmaceutical-like (methanol-, acetone- and propanol-contaminated) wastewater under low-temperature conditions (15 degrees C). The microbial diversity and diversity changes of the sludge samples were ascertained by applying 16S rRNA gene cloning and terminal restriction fragment length polymorphism (TRFLP) analyses, respectively, and using sludge samples from the inoculum, throughout and at the conclusion of the bioreactor trial. Data from genetic fingerprinting correlated well with those from physiological activity assays of the reactor biomass. Specifically, for example, TRFLP profiles indicated the dominance of hydrogenotrophic methanogens within the archaeal community, thus supporting the findings of specific methanogenic activity measurements. TRFLP data supported the hypothesis that the deviation between the replicated reactors, in terms of treatment efficiency, was associated with succession within the microbial communities present, and indicated that community development was linked to both operating temperature and wastewater composition. Fluorescence in situ hybridization (FISH) was also applied, to quantitatively assess the abundance of selected microbial groups, and revealed the underestimation of the abundance Methanosarcina by gene cloning analysis and demonstrated the spatial arrangement of these organisms within the architecture of the low-temperature solvent-degrading anaerobic biofilms. 相似文献
60.
为了探讨循环肿瘤细胞(circulating tumor cells,CTCs)在肺癌诊断中的应用及临床意义,本研究收集了我院2014年10月至2017年12月收治并确诊的肺癌患者87例、健康体检者40名以及肺部良性疾病患者50例作为研究对象,采用人循环肿瘤细胞试剂盒测定外周血CTC水平,两组间比较采用Mann-Whitney U检验,多组间比较采用Kruskal-Wallis检验,利用ROC曲线评价CTC在肺癌诊断上的灵敏度以及特异度。实验发现肺癌患者CTC水平(M=12.67 Unints/3 mL)显著高于肺部良性病患者(M=4.76 Unints/3 mL)和健康者(M=4.48 Unints/3 mL),差异存在统计学意义(p<0.001)。在不同性别、不同年龄段以及不同病理类型之间肺癌患者的CTC水平比较差异无统计学意义(p>0.05)。Ⅲ+Ⅳ期肺癌患者CTC水平显著高于Ⅰ+Ⅱ期患者,差异存在统计学意义(p<0.001)。有远处转移的肺癌患者CTC水平显著高于无远处转移的肺癌患者,差异存在统计学意义(p<0.001)。ROC曲线下面积为0.878(95%CI 0.820~0.936),临界值为6.34 Unints/3 mL,对应的灵敏度为0.77,特异度为0.989。本研究初步认为,CTC检测对肺癌诊断具有较高的灵敏度以及特异度,可能存在重要的临床应用价值。 相似文献