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91.
Anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) by a denitrifying bacterium 总被引:6,自引:0,他引:6
The anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) was studied in a denitrifying bacterium. Cells grown with
2-hydroxybenzoate were simultaneously adapted to degrade benzoate. Extract of these cells formed benzoate or benzoyl-CoA when
incubated under reducing conditions with salicylate, MgATP, and coenzyme A, suggesting a degradation of 2-hydroxybenzoate
via benzoate or benzoyl-CoA. This suggestion was supported by enzyme activity measurements. In extracts of 2-hydroxybenzoate-grown
cells, the following enzyme activities were detected: two CoA ligases, one specific for 2-hydroxybenzoate, the other for benzoate,
and two different enzyme activities catalyzing the reductive transformation of 2-hydroxybenzoyl-CoA. These findings suggest
a degradation of salicylic acid by two new enzymes, 2-hydroxybenzoate-CoA ligase (AMP-forming) and 2-hydroxybenzoyl-CoA reductase
(dehydroxylating), catalyzing (1) 2-hydroxybenzoate + MgATP + CoASH → 2-hydroxybenzoyl-CoA + MgAMP + PPi (2) 2-hydroxybenzoyl-CoA + 2[H] → benzoyl-CoA + H2O Benzoyl-CoA was dearomatized by reduction of the ring. This represents another case in which benzoyl-CoA is a central intermediate
in anaerobic aromatic metabolism.
Received: 1 February 1996 / Accepted: 24 February 1996 相似文献
92.
Kanika Misra Arun B. Banerjee Subhankar Ray Manju Ray 《Molecular and cellular biochemistry》1996,156(2):117-124
Two enzymes, one NADPH-dependent and another NADH-dependent which catalyze the reduction of methylglyoxal to acetol have been isolated and substantially purified from crude extracts of Escherichia coli K12 cells. Substrate specificity and formation of acetol as the reaction product by both the enzymes, reversibility of NADH-dependent enzyme with alcohols as substrates and inhibitor study with NADPH-dependent enzyme indicate that NADPH-dependent and NADH-dependent enzymes are identical with an aldehyde reductase (EC 1.1.1.2) and alcohol dehydrogenase (EC 1.1.1.1) respectively. The Km for methylglyoxal have been determined to be 0.77 mM for NADPH-dependent and 3.8 mM for NADH-dependent enzyme. Stoichiometrically equimolar amount of acetol is formed from methylglyoxal by both NADPH- and NADH-dependent enzymes. In phosphate buffer, both the enzymes are active in the pH range of 5.8–6.6 with no sharp pH optimum. Molecular weight of both the enzymes were found to be 100,000 ± 3,000 by gel filtration on a Sephacryl S-200 column. Both NADPH- and NADH-dependent enzymes are sensitive to sulfhydryl group reagents. 相似文献
93.
M. Popović S. KevreŠan J. Kandrač J. Nikolić N. Petrović R. Kastori 《Biologia Plantarum》1996,38(2):281-287
In young sugar beet plants cadmium suppressed the activity of nitrate reductase, glutamine synthetase and glutamate dehydrogenase,
whereas sulphur exhibited a protective role towards activity of these enzymes, except of glutamine synthetase. Protein synthesis
was suppressed in the absence of S in nutrient medium; the lowest level was at 10-3 M Cd2+. Chloroplast pigment contents were increased by S while Cd2+, even in the lowest concentration, (10−5 M) showed a repressive effect. The highest concentrations of Cd2+ (10−3 M) caused a decrease in dry mass, whereas S induced its increase. Nitrate content was increased in the presence of
Cd2+ and decreased by increased concentration of S.
Acknowledgement: The authors acknowledge financial support of the Ministry for Science and Technology of Serbia.
The paper was presented at 9th Congress of the Federation of European Societies of Plant Physiology, Brno, Czech Republic,
3–8 July 1994. 相似文献
94.
Ferredoxin-sulfite reductases (Fd-SiRs) [hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1] from leek leaves have been
purified to homogeneity. The enzymes (SiR 1, SiR 2 and SiR 3) were separated by Mono Q chromatography. The collective molecular
mass of the enzymes was estimated to be 65 kDa by gel filtration. In all three cases, subunit analysis by SDS-PAGE yielded
a single protein band corresponding to a molecular mass of 64 kDa, indicating that the enzymes each exist as a monomer. In
the oxidized forms, SiR 1, SiR 2 and SiR 3 all exhibited nearly identical absorption maxima at 279∼280, 389∼390, 588 and 714
nm, indicating that siroheme is involved in the catalysis of sulfite reduction. On enzymatic properties, SiR 1, SiR 2 and
SiR 3 could only react with the physiological electron donor, feriedoxin. The enzymes exhibited different heat stabilities.
The pH active curve obtained from SiR 2 was different from the others. Moreover, SiR 1 exhibited a lower Km value for ferredoxin
than SiR 2. Although the N-terminal sequence was the same, the results of some enzymatic properties, amino acid analysis,
and peptide mapping suggested the presence of the Fd-SiR isozymes in leek leaves. 相似文献
95.
本文报道人疱疹病豢-6型(HHV-6)pSTY28DNA片段的序列测定。应用分子克隆、缺损突变体(Dcletionmutant)制备和序列测定等技术,完成了3.9kbHHV-6pSTY28DNA片段的全序列测定。经DNASIS核酸蛋白软件分析,该片段含有两个开读框架(ORF)核糖核苷酸还原酶(RIR)ORF有2414个核苷酸,可编码805个氨基酸;P41蛋白由1100个核苷酸组成。与其他疱疹病毒作氨基酸同源性比较,HHV-6RiR与人巨细胞病毒(HCMV)有高度同源性,最适记分(Optimizedscore)达459。实验结果支持Esftathiou提出的论点,HHV-6属于β-疱疹病毒。 相似文献
96.
Paul W. Ludden 《Molecular and cellular biochemistry》1994,138(1-2):123-129
Several cases of ADP-ribosylation of endogenous proteins in procaryotes have been discovered and investigated. The most thoroughly studied example is the reversible ADP-ribosylation of the dinitrogenase reductase from the photosynthetic bacteriumRhodospirillum rubrum and related bacteria. A dinitrogenase reductase ADP-ribosyltransferase (DRAT) and a dinitrogenase reductase ADP-ribose glycohydrolase (DRAG) fromR. rubrum have been isolated and characterized. The genes for these proteins have been isolated and sequences and show little similarity to the ADP-ribosylating toxins. Other targets for endogenous ADP-ribosylation by procaryotes include glutamine synthetase inR. rubrum andRhizobium meliloti and undefined proteins inStreptomyces griseus andPseudomonas maltophila. 相似文献
97.
The localization of the dissimilatory sulfite reductase in Desulfovibrio desulfuricans strain Essex 6 was investigated. After treatment of the cells with lysozyme, 90% of the sulfite reductase activity was found in the membrane fraction, compared to 30% after cell rupture with the French press. Sulfite reductase was purified from the membrane (mSiR) and the soluble (sSiR) fractiion. On SDS-PAGE, both mSiR and sSiR exhibited three bands at 50, 45 and 11 kDa, respectively. From their UV/VIS properties (distinct absorption maxima at 391, 410, 583, 630 nm, enzymes as isolated) and the characteristic red fluorescence in alkaline solution, mSiR and sSiR were identified as desulfoviridin. Sulfite reductase (HSO3
-H2S) activity was reconstituted by coupling of mSiR to hydrogenase and cytochrome c
3 from D. desulfuricans. The specific activity of mSiR was 103 nmol H2 min-1 mg-1, and sulfide was the major product (72% of theoretical yield). No coupling was found with sSiR under these conditions. Furthermore, carbon monoxide was used to diferentiate between the membrane-bound and the soluble sulfite reductase. In a colorimetric assay, with photochemically reduced methyl viologen as redox mediator, CO stimulated the activity of sSiR significantly. CO had no effect in the case of mSiR. These studies documented that, as isolated, both forms of sulfite reductase behaved differently in vitro. Clearly, in D. desulfuricans, the six electron conversion HSO3
-H2S was achieved by a membranebound desulfoviridin without the assistance of artificial redox mediators, such as methyl viologen.Abbreviations
SiR
sulfite reductase
-
mSiR
sulfite reductase purified from membranes
-
sSiR
sulfite reductase purified from the soluble fraction
Enzymes Sulfite reductase, EC 1.8.99.1 Cytochrome c
3 hydrogenase, EC 1.12.2.1 相似文献
98.
Theerg3 gene ofNeurospora crassa was sequenced (EMBL accession no. X77955) and found to encode a protein of 490 amino acid residues with significant homology
to the yeast sterol biosynthetic enzyme C-14 reductase (39% identity) and also to the C-tenninal region in the sequence reported
for the chicken lamin B receptor (41% identity). The possibility that a single protein may possess both lamin B receptor and
sterol C-14 reductase functions might account for non-sterol-biosynthetic effects of mutations in sterol biosynthesis genes
and of inhibitors of sterol biosynthetic enzymes. 相似文献
99.
Yosepha Shahak Christof Klughammer Ulrich Schreiber Etana Padan Inge Herrman Günter Hauska 《Photosynthesis research》1994,39(2):175-181
The reduction by sulfide of exogenous ubiquinone is compared to the reduction of cytochromes in chromatophores of Rhodobacter capsulatus. From titrations with sulfide values for Vmax of 300 and 10 moles reduced/mg bacteriochlorophyll a·h, and for Km of 5 and 3 M were estimated, for decyl-ubiquinone-and cytochrome c-reduction, respectively. Both reactions are sensitive to KCN, as has been found for sulfide-quinone reductase (SQR) in Oscillatoria limnetica, which is a flavoprotein. Effects of inhibitors interfering with quinone binding sites suggest that at least part of the electron transport from sulfide in R. capsulatus employs the cytochrome bc
1-complex via the ubiquinone pool.Abbreviations BChl a
bacteriochlorophyll a
- DAD
diaminodurene
- decyl-UQ
decyl-ubiquinone
- LED
light emitting diode
- NQNO
2-n-nonyl-4-hydroxyquinoline-N-oxide
- PQ-1
plastoquinone 1
- SQR
sulfide-quinone reductase (E.C. 1.8.5.'.)
- UQ
ubiquinone 10
- Qc
the quinone reduction site on the cytochrome b
6
f/bc
1, complex (also termed Qi or Qr or Qn)
- Qs
the quinone reduction site on SQR
- Qz
quinol oxidation site on the b
6
f/bc
1, complex (also termed Qo or Qp) 相似文献
100.