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21.
In a previous laboratory study, larvae of the ant-tended lycaenid butterfly Hemiargus isola developed into larger adults when reared with the ant Formica perpilosa than when reared without ants. Ants neither fed butterfly larvae nor significantly delayed developmental duration. We investigated
two non-exclusive hypotheses for the mechanism of this effect: larvae tended by F. perpilosa (1) consume more food, and (2) digest the food they consume more efficiently, than those reared without ants. Larvae reared
in the laboratory with F. perpilosa ants became significantly heavier adults but produced a significantly lighter fecal mass than their untended counterparts,
suggesting that greater food consumption was not the primary mechanism for the higher growth rates of ant-tended larvae. Tended
and untended larvae were equally proficient at digesting the contents of pollen (a major natural food source) throughout the
tended portion of the life cycle. Taken together, the results suggest that neither greater consumption nor higher assimilation
accounts for the larger size of F. perpilosa-tended larvae. We propose that tended larvae may expend less energy than their untended counterparts.
Received: 3 January 1997 / Accepted: 18 June 1997 相似文献
22.
Synopsis The caloric density of stomach contents in juvenile chinook salmon,Oncorhynchus tshawytscha, was not affected by gastric evacuation, suggesting a constant caloric density of stomach contents during evacuation. Differences in the caloric density of prey consumed did affect caloric density of stomach contents over a 24-h period. Consumption of the amphipodCorophium sp. was associated with reduced caloric densities of stomach contents. During periods whenCorophium contributed more than 4% of the stomach contents, average caloric density declined from 5.56 to 5.33 kcal g–1. Despite this difference, estimates of daily energy intake of juvenile chinook salmon were only 3%, greater when developed from the mean caloric density of stomach contents excludingCorophium. 相似文献
23.
Arivalagan Pugazhendhi Sridevi Dhanarani Theivaraj Gowri Manogari Boovaragamoorthy Veerasamy Veeramani Kathirvel Brindhadevi Naif Abdullah Al-Dhabi Mariadhas Valan Arasu Thamaraiselvi Kaliannan 《Saudi Journal of Biological Sciences》2021,28(1):988
Tetracycline (TC) is one of the common antibiotics which is widely used in livestock growth promotion. The prevalent application TC may pave way to progression of antibiotic resistant bacteria. The main objective of this study is to determine the effect of Autothermal Thermophilic Aerobic Digestion (ATAD) on the fate of TC residues found in digested poultry litter. For the determination of TC in poultry litter, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were done. TLC result revealed that the Rf value of standard TC on TLC plate was 0.97 which correlates with the Rf value of TC at 0, 12, 24 and 36 h of digested poultry litter sample and not at 48, 60 and 72 h. HPLC chromatogram revealed that the limits of detection and the recovery were 5 µg/kg and 96% for standard TC. Linear correlation curves were obtained over the series of 100–500 µg/mL with correlation coefficient of 0.996 and the calibration curve was Y = 0.001X + 0.066. These results confirmed the degradation of TC in ATAD digestion of poultry litter by abiotic processes. 相似文献
24.
Hennemuth W Rhoads LS Eichelberger H Watanabe M Van Bell KM Ke L Kim H Nguyen G Jonas JD Veith D Van Bell CT 《The Journal of eukaryotic microbiology》2008,55(1):44-50
Abiotic factors are thought to be primarily responsible for the loss of bacteriophages from the environment, but ingestion of phages by heterotrophs may also play a role in their elimination. Tetrahymena thermophila has been shown to ingest and inactivate bacteriophage T4 in co-incubation experiments. In this study, other Tetrahymena species were co-incubated with T4 with similar results. In addition, T. thermophila was shown to inactivate phages T5 and lambda in co-incubations. Several approaches, including direct visualization by electron microscopy, demonstrated that ingestion is required for T4 inactivation. Mucocysts were shown to have no role in the ingestion of T4. When (35)S-labeled T4 were fed to T. thermophila in a pulse-chase experiment, the degradation of two putative capsid proteins, gp23(*) and hoc, was observed. In addition, a polypeptide with the apparent molecular mass of 52 kDa was synthesized. This suggests that Tetrahymena can use phages as a minor nutrient source in the absence of bacteria. 相似文献
25.
Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System 总被引:1,自引:0,他引:1
Pedro M. Reis Thomas W. Raab Jean Y. Chuat Martin E. Leser Reinhard Miller Heribert J. Watzke Krister Holmberg 《Food biophysics》2008,3(4):370-381
In the present study, we use a model gastro-intestinal system to study the influence of different food-grade surface-active
molecules (Sn-2 monopalmitin, β-lactoglobulin, or lysophosphatodylcholine) on lipase activity. The interfacial activity of
lipase and surfactants are assessed with the pendant drop technique, a commonly used tensiometry instrument. A mathematical
model is adopted which enables quantitative determination of the composition of the water–oil interface as a function of bulk
surfactant concentration in the water–oil mixtures. Our results show a decrease in gastric lipolysis when interfacially active
molecules are incorporated into a food matrix. However, only the Sn-2 monopalmitin caused a systematic decrease in triglyceride
hydrolysis throughout the gastro-intestinal tract. This effect is most likely due to exclusion of both lipase and triglyceride
from the water–oil interface together with a probable saturation of the solubilization capacity of bile with monoglycerides.
Addition of β-lactoglobulin or lysophopholipids increased the hydrolysis of fat after the gastric phase. These results can
be attributed to an increasing interfacial area with lipase and substrate present at the interface. Otherwise, β-lactoglobulin,
or lysophopholipids reduced fat hydrolysis in the stomach. From the mathematical modeling of the interface composition, we
can conclude that Sn-2 monopalmitin can desorb lipase from the interface, which, together with exclusion of substrate from
the interface, explains the gradually decreased triglyceride hydrolysis that occurs during the digestion. Our results provide
a biophysics approach on lipolysis that can bring new insights into the problem of fat uptake. 相似文献
26.
Light microscopy of native preparations, histology, and electron microscopy have revealed that Phlebotomus duboscqi belongs to a class of sand fly species with prompt development of the peritrophic matrix (PM). Secretion of electron-lucent
fibrils, presumably chitin, starts immediately after the ingestion of a blood meal and, about 6 h later, is followed by secretion
of amorphous electron-dense components, presumably proteins and glycoproteins. The PM matures in less than 12 h and consists
of a thin laminar outer layer and a thick amorphous inner layer. No differences have been found in the timing of the disintegration
of the PM in females infected with Leishmania major. In both groups of females (infected and uninfected), the disintegration of the PM is initiated at the posterior end. Although
parasites are present at high densities in the anterior part of the blood meal bolus, they escape from the PM at the posterior
end only. These results suggest that L. major chitinase does not have an important role in parasite escape from the PM. Promastigotes remain in the intraperitrophic space
until the PM is broken down by sand-fly-derived chitinases and only then migrate anteriorly. Disintegration of the PM occurs
simultaneously with the morphological transformation of parasites from procyclic forms to long nectomonads. A novel role is
ascribed to the anterior plug, a component of the PM secreted by the thoracic midgut; this plug functions as a temporary barrier
to stop the forward migration of nectomonads to the thoracic midgut.
This work was supported by the Ministry of Education of the Czech Republic (projects MSM0021620828 and LC06009). 相似文献
27.
28.
In this work, polydopamine‐coated magnetic graphene (MG@PDA) nanocomposites were synthesized by a facile method. Trypsin was then directly immobilized on the surface of the nanocomposites through simple PDA chemistry with no need for introducing any other coupling groups. The as‐made MG@PDA nanocomposites inherit not only the large surface area of graphene which makes them capable of immobilizing high amount of trypsin (up to 0.175 mg/mg), but also the good hydrophilicity of PDA which greatly improves their biocompatibility. Moreover, the strong magnetic responsibility makes them easy to be separated from the digested peptide solution when applying a magnetic field. The feasibility of the trypsin‐immobilized MG@PDA (MG@PDA‐trypsin) nanocomposites for protein digestion was investigated and the results indicated their high digestion efficiency in a short digestion time (10 min). In addition, the reusability and stability of the MG@PDA‐trypsin nanocomposites were also tested in our work. To further confirm the efficiency of MG@PDA‐trypsin nanocomposites for proteome analysis, they were applied to digest proteins extracted from skimmed milk, followed by nano RPLC‐ESI‐MS/MS analysis, and a total of 321 proteins were identified, much more than those obtained by 16‐h in‐solution digestion (264 proteins), indicating the great potential of MG@PDA‐trypsin nanocomposites as the supports for high‐throughput proteome study. 相似文献
29.
30.
Sulfakinin (SK) is a sulfated insect neuropeptide that is best known for its function as a satiety factor. It displays structural and functional similarities with the vertebrate peptides gastrin and cholecystokinin. Peptidomic studies in multiple insects, crustaceans and arachnids have revealed the widespread occurrence of SK in the arthropod phylum. Multiple studies in hemi- and holometabolous insects revealed the pleiotropic nature of this neuropeptide: in addition to its activity as a satiety factor, SK was also reported to affect muscle contraction, digestive enzyme release, odor preference, aggression and metabolism. However, the main site of action seems to be the digestive system of insects. In this study, we have investigated whether SK can intervene in the control of nutrient uptake and digestion in the migratory locust (Locusta migratoria). We provide evidence that sulfakinin reduces food uptake in this species. Furthermore, we discovered that SK has very pronounced effects on the main digestive enzyme secreting parts of the locust gut. It effectively reduced digestive enzyme secretion from both the midgut and gastric caeca. SK injection also elicited a reduction in absorbance and proteolytic activity of the gastric caeca contents. The characteristic sulfation of the tyrosine residue is crucial for the observed effects on digestive enzyme secretion. In an attempt to provide potential leads for the development of peptidomimetic compounds based on SK, we also tested two mimetic analogs of the natural peptide ligand in the digestive enzyme secretion assay. These analogs were able to mimic the effect of the natural SK, but their effects were milder. The results of this study provide new insights into the action of SK on the digestive system in (hemimetabolous) insects. 相似文献