全文获取类型
收费全文 | 150篇 |
免费 | 3篇 |
国内免费 | 5篇 |
出版年
2022年 | 1篇 |
2021年 | 2篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 3篇 |
2012年 | 7篇 |
2011年 | 7篇 |
2010年 | 2篇 |
2009年 | 4篇 |
2008年 | 6篇 |
2007年 | 2篇 |
2006年 | 5篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 6篇 |
2001年 | 5篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 8篇 |
1996年 | 3篇 |
1995年 | 8篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 4篇 |
1987年 | 8篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1967年 | 1篇 |
排序方式: 共有158条查询结果,搜索用时 15 毫秒
31.
Senescence of carnation (Dianthus caryophyllus L. ev. White Sim) petals coincided with a decrease on a per flower basis in the yield of cell wall and ethanol-insoluble solids. The decrease in cell wall yield per flower was due largely to a loss of neutral sugars, primarily galactose (45%) and arabinose (23%). On a per flower basis, water-and chelator-soluble pectins increased throughout development, comprising in senescent petals 18 and 58%, respectively, of total pectin. Alkali-soluble pectins ranged from 35 to 45% of the total pectin and decreased during senescence. Gel chromatography of chelator- and alkali-soluble pectins revealed no change in molecular size and polygalacturonase activity was not detected. Large-molecular-size hemicelluloses decreased during development, an observation reminiscent of the changes affecting hemicelluloses during the ripening of a number of fruit types. Compositional analysis of the large hemicellulosic polymers revealed a decrease in xylose and galactose content. 相似文献
32.
Five hours after cut carnations had been treated with a pulse of 1 or 4 mM silver thiosulfate (STS), in vivo ethylene binding in petals was inhibited by 22 and 29%, respectively. When binding was measured 4 days after the 4-mM STS treatment, binding was inhibited by 81%. 2,5-Norbornadiene, which substantially delays carnation senescence, inhibited ethylene binding by 41% at a concentration of 1000 l/l. The Kd for ethylene binding in carnations was estimated to be 0.1 l/l in petals and 0.09 l/l in leaves. The concentration of binding sites was estimated to be 6.0×10–9 mol/kg of petals and 2.0×10–9 mol/kg of leaves 相似文献
33.
The senescence of flower petals is a highly regulated developmental process which requires active gene expression and protein synthesis. The biochemical changes associated with petal senescence in carnation flowers include an increase in hydrolytic enzymes, degradation of macro-molecules, increased respiratory activity and a climacteric-like increase in ethylene production. It is clear that the gaseous phytohormone ethylene plays a critical role in the regulation and coordination of senescence processes. Many reviews on physiology and mode of action of ethylene are available. Molecular cloning led to the isolation of genes involved in ethylene biosynthesis and action. This review describes the current status of the studies on regulation of ethylene biosynthesis and ethylene response in carnation flowers. An overview is given of studies on senescence-related gene expression and possibilities to improve postharvest longevity by genetic engineering.Abbreviations ACC
1-aminocyclopropane-1-carboxylic acid
- AIB
-amino-isobutyric acid
- AOA
amino oxyacetic acid
- AVG
aminoathoxyvinyl glycine
- DACP
diazocyclopentadiene
- EFE
ethylene forming enzyme
- MACC
malonyl 1-aminocyclopropane-1-carboxylic acid
- MTA
5-methylthio-adenosine
- NBD
2,5 norbornadiene
- ppb
parts per billion
- SAM
S-adenosyl-methionine
- STS
silver thiosulphate 相似文献
34.
The relative distribution of phytoalexins induced by Fusarium oxysporum fsp. dianthi in carnation and localized by pyrolysis-mass spectrometry in the xylem differs from that found by HPLC in acetone extracts. In situ, in the xylem, there is a predominance of 2-aryl-1,3-benzoxazin-4H-ones over the more fungitoxic N-aroylanthranilates. At least part of the dianthramides in the extracts has to be ascribed to artifacts apparently due to hydrolysis of benzoxazinones. 相似文献
35.
J. Van Staden B. C. Featonby-Smith S. Mayak H. Spiegelstein A. H. Halevy 《Plant Growth Regulation》1987,5(2):75-86
Tentative identification using HPLC and RIA techniques indicated the presence of zeatin-O-glucoside, zeatin, ribosylzeatin, dihydrozeatin, iso-pentenyladenine and iso-pentenyladenosine in the petals of carnation flowers. Dihydrozeatin is apparently responsible for most of the biological activity. Within the petals most activity was detected in the basal parts which also senesced much slower than the upper parts of the petals. Treatment with AOA extended petal longevity and reduced ethylene production. This was associated with higher cytokinin-like activity in the basal parts of the petals.These higher levels of cytokinins were not observed in the petals of ACC treated flowers or in the detached control flowers. It is suggested that cytokinin transport and/or metabolism may play an important role in regulating ethylene production in cut carnations. 相似文献
36.
H. Friedman A. Hagiladi N. Resnick A. Barak N. Umiel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):282-287
Within the genetically variable population of Dianthus barbatus’’Dagan’’, we identified genetic lines with flowers possessing ethylene insensitivite-related phenotypes. The phenotypes are: Caf
+
, with a fresh-looking corolla attached to a mature ovary; Inr
–
, with flowers whose petals do not inroll during flower senescence; and Rfi
+
, whose flower petals inroll, but recover from inrolling. The frequencies of Caf
+
, Inr
-
and Rfi
+
were 33%, 25% and 75% in the population inspected, respectively. These relatively high frequencies were probably due to continuous
selection over the years for flowers with open and long-lasting corollas. By examining the distribution of the genetic lines
which express two of these phenotypes, we determined that Caf
+
, Inr
–
and Rfi
+
are independent traits. However, these traits might be associated with male sterility. Exposure of a random sample of the
population to exogenous ethylene, followed by examination of the resulting inrolling and wilting, revealed five different
ethylene response groups. In one group ethylene enhanced both inrolling and wilting, and most genetic lines within this group
exhibited a Caf
–
phenotype. In two other groups ethylene enhanced either the inrolling or the wilting, and both of the Caf
+
and Caf
–
phenotypes were included. Two other groups were completely non-responsive to ethylene, but in one of them the flower life
span was twice as long as in the other, and all the genetic lines exhibited the Caf
+
phenotype. We concluded that the Caf
+
phenotype is most-likely related to ethylene insensitivity and that the inrolling and wilting are controlled by ethylene,
probably via different pathways.
Received: 25 June 2000 / Accepted: 15 November 2000 相似文献
37.
A rationale for the shift in colour towards blue in transgenic carnation flowers expressing the flavonoid 3',5'-hydroxylase gene 总被引:6,自引:0,他引:6
Recently marketed genetically modified violet carnations cv. Moondust and Moonshadow (Dianthus caryophyllus) produce a delphinidin type anthocyanin that native carnations cannot produce and this was achieved by heterologous flavonoid 3',5'-hydroxylase gene expression. Since wild type carnations lack a flavonoid 3',5'-hydroxylase gene, they cannot produce delphinidin, and instead accumulate pelargonidin or cyanidin type anthocyanins, such as pelargonidin or cyanidin 3,5-diglucoside-6"-O-4, 6"'-O-1-cyclic-malyl diester. On the other hand, the anthocyanins in the transgenic flowers were revealed to be delphinidin 3,5-diglucoside-6"-O-4, 6"'-O-1-cyclic-malyl diester (main pigment), delphinidin 3,5-diglucoside-6"-malyl ester, and delphinidin 3,5-diglucoside-6",6"'- dimalyl ester. These are delphinidin derivatives analogous to the natural carnation anthocyanins. This observation indicates that carnation anthocyanin biosynthetic enzymes are versatile enough to modify delphinidin. Additionally, the petals contained flavonol and flavone glycosides. Three of them were identified by spectroscopic methods to be kaempferol 3-(6"'-rhamnosyl-2"'-glucosyl-glucoside), kaempferol 3-(6"'-rhamnosyl-2"'-(6-malyl-glucosyl)-glucoside), and apigenin 6-C-glucosyl-7-O-glucoside-6"'-malyl ester. Among these flavonoids, the apigenin derivative exhibited the strongest co-pigment effect. When two equivalents of the apigenin derivative were added to 1 mM of the main pigment (delphinidin 3,5-diglucoside-6"-O-4,6"'-O-1-cyclic-malyl diester) dissolved in pH 5.0 buffer solution, the lambda(max) shifted to a wavelength 28 nm longer. The vacuolar pH of the Moonshadow flower was estimated to be around 5.5 by measuring the pH of petal. We conclude that the following reasons account for the bluish hue of the transgenic carnation flowers: (1). accumulation of the delphinidin type anthocyanins as a result of flavonoid 3',5'-hydroxylase gene expression, (2). the presence of the flavone derivative strong co-pigment, and (3). an estimated relatively high vacuolar pH of 5.5. 相似文献
38.
39.
40.
The influence of culture filtrates of Fusarium oxysporum f.sp. dianthi which causes Fusarium wilt was investigated on growth and viability of carnation tissue cultures and leaf segments. Culture filtrates of avirulent race 1 of this fungus did not affect calli and leaf segments of cultivars both susceptible and resistant to Fusarium wilt. However, culture filtrates of virulent race 2 decreased viability and suppressed growth of callus of the susceptible cultivar. In contrast, callus of the resistant cultivar showed resistance to the culture filtrates. The results of these experiments may provide information on methods of selection of new wilt resistant carnation varieties.Abbreviations A270
absorbance at 270 nm
- 2,4-d
2,4-dichlorophenoxyacetic acid
- CF-MCD
culture filtrate of 16064 grown in MCD medium
- MCD medium
modified Czapeck-Dox medium
- MS medium
basal medium of Murashige and Skoog
- MW
molecular weight
- PD medium
potato dextrose medium
- TTC
2,3,5-triphenyl tetrazolium chloride 相似文献