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11.
用抗单纯疱疹病毒(HSV)型共同性gC和gD羊克隆抗体(McAb),包被即Eppendorf管,捕捉HSV,同时加入3个引物:一个是HSV─1/HSV─2型共同性上游引物,另两个分别是HSV─1和HSV─2型特异性下游引物。借此建立了能直接分型检测HSV的抗原捕获聚合酶链式反应(AC─PCR)。HSV─1的扩增产物为477bp,HSV─2的为399bp两型病毒经AC─PCR扩增后产生分子量不同的DNA片段,致使AC─PCR能直接分型检测HSV。HSV─1和HSV─2扩增产物的克隆和序列分析表明,本方法特异性好。用本法检测Balb/c幼鼠中枢神经系统HSV感染的脑标本,进一步证实本方法不仅敏感、特异,而且分型准确。 相似文献
12.
结构化设计思想及Quick BASIC编程环境在实验设计和数据管理中的应用 总被引:2,自引:1,他引:1
封多佳 《微生物学免疫学进展》1995,23(3):155-157
结构化设计思想及MSDOS(V5.0)中的QuickBASIC编程环境通常用于计算机程序设计。作者利用其合理的设计思想及其优越的编辑环境,不需编程或购置更多的软件即能方便地进行实验设计、实验数据管理、文献检索及论文编辑等,可提高实验设计的逻辑性及实验数据管理的效率。 相似文献
13.
Michael W. Palmer 《植被学杂志》1995,6(3):447-447
Abstract. This note is to apologize for an error in the computer program used to evaluate the random data used in Fuzzy Set Ordination according to Zhang & Oxley. After correction of this error no artifacts could be detected any longer. However, the basic conclusion of the earlier critical note still stands: if one selects environmental variables after analyzing the results of a multivariate gradient analysis, and then uses these variables as input into a multiple univariate gradient analysis, the results are expected to be comparable. 相似文献
14.
Summary NMR View is a computer program designed for the visualization and analysis of NMR data. It allows the user to interact with a practically unlimited number of 2D, 3D and 4D NMR data files. Any number of spectral windows can be displayed on the screen in any size and location. Automatic peak picking and facilitated peak analysis features are included to aid in the assignment of complex NMR spectra. NMR View provides structure analysis features and data transfer to and from structure generation programs, allowing for a tight coupling between spectral analysis and structure generation. Visual correlation between structures and spectra can be done with the Molecular Data Viewer, a molecular graphics program with bidirectional communication to NMR View. The user interface can be customized and a command language is provided to allow for the automation of various tasks.Inquiries concerning the availability of NMR View and the Molecular Data Viewer should be sent via email to johnsonb@merck.com or to Bruce A. Johnson, Merck Research Laboratories, RY80Y-103, P.O. Box 2000, Rahway, NJ 07065, U.S.A. 相似文献
15.
J. Sabater Pi M. Bermejo G. Illera J. J. Vea 《International journal of primatology》1993,14(5):797-804
For the first time, three cases of capture and forced interaction were observed between bonobos (Pan paniscus)and two other species of primates (Colobus angolensisand Cercopithecus ascanius)in the Lilungu (Ikela) region, Republic of Zaire. The bonobos interacted with the captured primates as if they were dealing with individuals of their own species. They sought cooperation in their interactions with the captured young primates without scccess. There is no evidence that they ate the captives. 相似文献
16.
带鱼是中国东海的重要经济渔获种类,其可捕规格会随不同时期的捕捞强度而变化。为确定目前东海带鱼最适可捕规格,根据2016—2020年拖网、帆张网及延绳钓等主捕带鱼作业方式采集的带鱼样品和数据,利用FiSAT II软件对其生长、死亡参数进行估算,进而计算可捕规格。结果表明: 带鱼的拐点肛长、临界肛长、1龄鱼肛长分别为382.84、397.12、216.05 mm,50%带鱼性成熟肛长为230.38 mm,最小可捕肛长为219.23 mm。利用Beverton-Holt模型分析带鱼单位补充量渔获量可知,带鱼当前渔业参考点为:tc=0.38 a,F=2.11,处于捕捞过度区域,并得出最适可捕肛长为364.64 mm。但过高的可捕规格会导致短期内带鱼产量急剧下降,渔民难以接受,因此,本研究综合考虑采用接近1龄鱼肛长与50%性成熟肛长作为最适可捕规格较为合适,即220 mm。 相似文献
17.
Nabeel A Affara 《Briefings in Functional Genomics and Prot》2003,2(1):7-20
DNA microarray technology permits the study of biological systems and processes on a genome-wide scale. Arrays based on cDNA clones, oligonucleotides and genomic clones have been developed for investigations of gene expression, genetic analysis and genomic changes associated with disease. Over the past 3-4 years, microarrays have become more widely available to the research community. This has occurred through increased commercial availability of custom and generic arrays and the development of robotic equipment that has enabled array printing and analysis facilities to be established in academic research institutions. This brief review examines the public and commercial resources, the microarray fabrication and data capture and analysis equipment currently available to the user. 相似文献
18.
植物标本标签的计算机印制数据系统 总被引:2,自引:2,他引:0
本文讨论了主要用以印制标本采集记录标签的数据系统及其数据库建库方案与编程原理。该系统可在IBM及其兼容系列个人计算机上使用,适合用于个人或科研与教学机构中小型标本采集信息管理;可用以数据的检索、标本标签的印制和植物名录的打印;也可用以地区性的植物区系与生态学研究。 相似文献
19.
The autogamous diploid annualMicroseris douglasii of California occurs in many isolated populations. The populations consist of one to many highly inbred biotypes. Morphological variation among populations usually is greater than within populations. In spite of the virtual absence of gene flow even within populations, genetically determined character differences are randomly distributed and associated throughout the range of the species. Recent evidence even suggests introgression of chloroplasts from the relatedM. bigelovii. Offspring families from 25 plants of a very variable population were raised and examined for segregation of morphological and molecular (RAPD) markers. All 25 original plants were completely homozygous for all markers, but each differed from all others at least in some markers. The population consisted of two genetically isolated groups of plants: a distinct inbred line (3 plants) and 22 plants with random associations of a common set of markers and characters, possibly recombinant inbreds from a past hybridization event. One of these 22 plants contained a chloroplast genome found inM. bigelovii, the other 24 plants a chloroplast genome found only inM. douglasii. 相似文献
20.
B. Thompson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1978,52(5):201-207
Summary Examples are presented to illustrate some of the effects aberrant values, in particular, measurement errors, may have on estimates of the genetic parameters related to selection studies. It is shown that aberrant values may cause observed response to selection pressure to differ considerably from predicted response. Possible dangers of indiscriminate screening are also discussed. 相似文献