Impact of Paecilomyces lilacinus Inoculum Level and Application Time on Control of Meloidogyne incognita on Tomato

Microplot experiments were conducted to evaluate the effects of inoculum level and time of application of Paecilomyces lilacinus on the protection of tomato against MeIoidogyne incognita. The best protection against M. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase in tomato yield, respectively, compared with tomato plants treated with this nematode alone. Greatest protection against this pathogen was attained when P. lilacinus was delivered into soil 10 days before planting and again at planting. Yield was increased twofold compared with yield in nematode-alone plots and plots with M. incognita plus the fungus. Percentages of P. lilacinus-infected egg masses were greatest in plots treated at midseason or at midseason plus an early application, compared with plots treated with the fungus 10 days before planting and (or) at planting time.     

Screening Subterranean Clover (Trifolium spp.) Germplasm for Resistance to Meloidogyne species

This study was conducted to identify lines of subterranean clover (Trifolium spp.) with resistance to Meloidogyne arenaria (Neal, 1989) Chitwood, 1949, race 1; M. incognita (Kofoid and White, 1919) Chitwood, 1949, race 3; and M. javanica (Treub, 1885) Chitwood, 1949. A collection of 134 subterranean clover lines was evaluated and all had intermediate to high susceptibility. Root galling was negatively correlated with both seed and dry matter yields. Soil fumigation significantly reduced the nematode population in the field. Results indicate there is limited genetic resistance to root-knot nematodes among subterranean clover lines. Alternative sources of variation for this trait should be investigated.     

Characterization of sialic acids containing lipopolysaccharide from Rhizobium meliloti M 11 S

Abstract The lipopolysaccharide (LPS) of Rhizobium meliloti strain M 11 S was isolated and analyzed. It contained fatty acids (3-hydroxymyristic, palmitic, stearic, arachidic acids) and sugars: glucose, galactose, glucosamine, 3-deoxy- d -mannooctulosonic acid and sialic acids (NeuAc, 9- O -acetyl-NeuAc) identified by combined gas-liquid-chromatography/ mass spectrometry (GLC-MS).     

Chromosomal evolution withinPiperaceae

Chromosome numbers for 26 different species of the generaPiper, Peperomia andPothomorphe (Piperaceae) are reported. The basic chromosome numbers are 2n = 26, x = 13 (Piper, Pothomorphe) and 2n = 22, x = 11 (Peperomia), polyploid series are characteristic forPiper andPeperomia. Piper has the smallest chromosomes and prochromosomal interphase nuclei,Peperomia the largest ones and mostly reticulate to euchromatic nuclei.Pothomorphe is intermediate in both characters. The karyomorphological differences betweenPothomorphe andPiper underline their generic separation. Interspecific size variation of chromosomes occurs inPiper andPeperomia. Infraspecific polyploidy was observed inPiper betle. C-banding reveals different patterns of heterochromatin (hc) distribution between the genera investigated. The genome evolution is discussed.     

Vectors permitting visual monitoring of simple transposition events

The construction and use of two novel transposon(Tn)-delivery vectors is described. These vectors carry Inc.W or Inc.N broad-host-range transfer functions cloned next to the narrow-host-range replicon of pBR329. The host specificities of pSLX10 and pSLX23 both complement and extend the host specificities of existing Tn delivery vectors. Plasmids pSLX10 and pSLX23 were shown to transfer at high frequency in intergeneric matings. The lux genes which are present on each vector permit the visual monitoring of transconjugants which have retained a Tn element, but are devoid of plasmid molecules. pSLX10 and pLSX23 were efficiently used to generate a range of auxotrophic mutants in various strains of Pseudomonas as well as to clone genes from Serratia liquefaciens. These vectors may have general applicability to identify and clone genes in a wide range of Gram-negative bacteria.     

The habitat diversity and fish reproductive function of floodplain ecosystems

Synopsis Fish reproduction in floodplain ecosystems, based on relative abundance and total biomass of 0+ juveniles, was studied using the synchronic approach to typological analysis in conjunction with Point Abundance Sampling by modified electrofishing. In 3 different flood plains of the Upper Rhône River, 1015 point samples yielding 4573 juveniles (0+) from 21 species were collected from 48 ecosystems of various geomorphological origin. The results demonstrate the lotic-to-lentic succession of floodplain ecosystems to be a series of non-sequential reproductive zones, with spawning conditions being reflected by the specific composition and guild structure of the YOY fish assemblages. The habitat diversity and the fish reproductive potential of floodplain ecosystems are strongly influenced by geomorphological origin and by past and present hydrological conditions. The YOY assemblages of autogenically driven ecosystems (usually of anastomose or meander origin) tend to differ both in composition and in quantity from those found in allogenically driven ecosystems (generally of braided origin). Ecosystems of intermediate character, and fish reproduction thereof, occur as the result of either ecosystem rejuvenation or senescence: autogenically driven ecosystems by allogenic mechanisms, or allogenically driven ecosystems by anthropic and/or autogenic mechanisms, respectively. Because of co-occurrence of ecosystems at similar and at different successional status, the flood plain as an entity is seen as stable with respect to fish reproduction.     

The perennial taxa ofCrucianella (Rubiaceae) in SW. Asia and their eco-geographical differentiation

The perennial taxa ofCrucianella in Asia form a coherent group, apparently diploid (x = 11) and outbreeding throughout, and should be placed into sect.Roseae. This Irano-Turanian group has its centre of diversity in the mountain systems south of the Caspian Sea and reaches with outposts NE. and E. Anatolia, NE. Iraq, S. Iran and C. Asia. Four species and 13 subspecies (within the polymorphicC. gilanica) are recognized, described (partly as new), and illustrated (Figs. 1–6). Conspectus, keys and distribution maps (Figs. 7 and 8) as well as plesio- and apomorphic character states and data on size of areas are provided (Table 1). There is an obvious correlation between more plesiomorphic taxa with smaller areas in the distribution centre of the group, and more apomorphic taxa with larger areas towards its periphery (Fig. 9). These findings are linked to a working hypothesis on the evolution of the group.Dedicated to Prof. DrLothar Geitler on the occasion of his 90th birthday.     

Chorology of the Euxinian and Hyrcanian element in the woody flora of Asia

The Euxinian and the Hyrcanian floristic province are analysed in respect to their trees and shrubs. These provinces mark the southern limit of the Euro-Siberian Region in SW. Asia. Mesophyllic forests dominate; they are deciduous, but there is a substantial component of evergreen shrubs in the understorey. Characteristics species frequently have a relic nature. Species lists and some exemplary distribution maps are presented for the Euxinian and the Hyrcanian element, both with endemic and more wide-spread species, including the characteristic Euxine-Hyrcanian group.     

Evolution of the Australian flora through the Tertiary

The evolution of the Australian flora through the Tertiary has not been reviewed by a megafossil palaeobotanist for almost a century. Based on material available and published studies, the Australian Tertiary can be considered in three units: Eocene, Oligo-Miocene, and Pliocene. Key taxa in the Eocene includeCasuarinaceae, Proteaceae, Fagaceae, Podocarpaceae, andLauraceae. Many known Eocene deposits are interpreted as warm, humid rainforests.Oligo-Miocene floras reflect a climatic deterioration, with greater occurrence of sclerophylly and xerophylly. A reduction of tropical taxa is seen with an increase ofMyrtaceae and earliest records ofMimosaceae, Chenopodiaceae, andPoaceae. These trends continue into the Pliocene, which is not well represented in Australia.Problems needing to be addressed include the discrepancy between the pollen and megafossil records ofNothofagus, the origins of Australia's wet tropical taxa, and the role of fire in the early evolution of the Australian flora.     

Body shape and differences between species

The variation of body shape among prosimians is reviewed. Special emphasis is placed on the selective advantages, that is the mechanical reasons, to which variants of the locomotor apparatus can be traced back. There are differences found in the cheiridia, but at present they cannot be explained in terms of mechanics; there is nearly no knowledge about the mechanical meaning of their diversity. Myological characteristics of taxa can be explained mechanically, but this has not yet been done. Well known are variations of body proportions. These discriminate higher taxa, and are largely coincident with the often-used locomotor categories. In spite of this, there are only few sound arguments about the real biomechanic value of characteristic proportions for a given locomotor mode. What is known on this field, is reviewed. Progress can be made only, if the mechanical conditions, set by postural behavior and locomotion, are understood completely. The subtle distinctions between lower taxonomic units can normally be identified only on the basis of detailed and quantified analyses of movements on one hand, and of biometrics on the other. In the few cases in which such studies have been made, the differences of morphology fit to the mechanical requirements of locomotion which also differ only in quantitative details.