Contrasting genetic and morphological differentiation among geographical lineages of a stenotopic miniature rasborine,Boraras maculatus,in Peninsular Malaysia

The variability in the stenotopic miniature rasborine Boraras maculatus (Cypriniformes: Danionidae: Rasborinae) across acidic-water habitats of Peninsular Malaysia (PM) was investigated using two molecular markers (the mitochondrial cytochrome c oxidase subunit I [COI] gene and the nuclear rhodopsin gene), as well as morphological evidence. Molecular phylogenetic analyses revealed differentiation among populations of B. maculatus in PM with the distinction of four allopatric lineages. Each of them was recognized as a putative species by automatic species delimitation methods. These lineages diverged from each other between 7.4 and 1.9 million years ago. A principal component analysis (PCA) was conducted to examine the multivariate variation in 11 morphometric measurements among three of these lineages. PCA results showed a significant overlap in morphological characteristics among these lineages. Additionally, a photograph-based machine learning approach failed to fully differentiate these lineages, suggesting limited morphological differentiation. B. maculatus represents a case of morphological stasis in a stenotopic miniature species. Strong habitat preference, coupled with long-term habitat fragmentation, may explain why each lineage of B. maculatus has a restricted distribution and did not disperse to other regions within and outside of PM, despite ample possibilities when the Sunda shelf was emerged and drained by large paleodrainages for most of the past 7 million years. The conservation status of B. maculatus and its peat swamp habitats are discussed, and it is concluded that peat swamps comprise several evolutionary units. Each of these units is considered a conservation unit and deserves appropriate protection.     

Two new freshwater fish species of the genus Telestes (Actinopterygii, Cyprinidae) from karst poljes in Eastern Herzegovina and Dubrovnik littoral (Bosnia and Herzegovina and Croatia)

Two new species, Telestes dabar and Telestes miloradi, are described on the basis of morphological comparisons of isolated geographical populations of fishes identified earlier as Telestes metohiensis. A lectotype is designated for Telestes metohiensis, whose range is shown to include waters of Gatačko, Cerničko, and Nevesinjsko poljes in Eastern Herzegovina. Telestes dabar from Dabarsko Polje (Eastern Herzegovina) and Telestes miloradi from Konavosko Polje (south Croatia) share with Telestes metohiensis the following combination of characters that distinguish them from the rest of the genus Telestes: pharyngeal teeth in one row, usually 5–4; preoperculo-mandibular canal not communicating with the infraorbital canal; mouth subterminal, the tip of the mouth cleft on or below the level of the ventral margin of the eye; postcleithrum minute or absent; ventral portion of the trunk with a dark stripe on a pale background; and dorsal portion of trunk uniformly dark and bordered ventrally by a dark midlateral stripe. Telestes dabar and Telestes miloradi are distinguishable from Telestes metohiensis in usually having 8½ branched dorsal-fin rays (vs. usually 7½), 9 or 10 gill rakers (vs. 7–10, usually 8), and the dark stripe on the ventral portion of the trunk below the main pigmented area of the back narrow and usually not reaching posteriorly to the caudal peduncle (vs. dark stripe wide and extending posteriorly to the caudal peduncle). Telestes dabar is distinguished from Telestes miloradi by having scales on most of the body situated close to one another and overlapping in a region behind the pectoral girdle and usually on the caudal peduncle (vs. overlapping scales on most of the body); the lateral line usually incomplete and interrupted, with 24–69, usually 54–65, total scales (vs. lateral line usually complete, with 55–67 total scales); scales above and below the lateral line slightly smaller than lateral-line scales (vs. of about equal size); head width 43–52% HL (vs. 48–58% HL); and lower jaw length 10–12% SL or 36–41% HL (vs. 8–10% SL or 33–38% HL). Telestes miloradi, a very local endemic species,is known only by historical samples. Telestes dabar is an abundant fish in Dabarsko Polje, but its range is critically restricted during the dry season by a few permanent sources. Nothing is known about its occurrence in underground karst waters.     

中国云南高原鳅属洞穴盲鱼一新种——邱北盲高原鳅

2002年和2006年,在云南省邱北县腻脚村一喀斯特洞穴深处各采到一批盲鳅标本,经鉴定,为高原鳅属(Triplophysa)一未记录的新种--邱北盲高原鳅。新种具有的特殊头形及可数性状等特征,与中国已记录的穴居高原鳅均不同,而与分布于金沙江水系的前鳍高原鳅(T.anterodorsalis Cao et Zhu)相近,但新种有下列特征可与之相区别:1)无眼vs.眼正常;2)背鳍分枝鳍条7vs.8-9;3)尾叉形vs.尾微凹。新种与同水系(南盘江)的大斑高原鳅(T.macromaculata Yang)也较相近,但可与之相区别:1)无眼vs.有眼;2)无色素vs.有斑;3)尾叉形vs.尾微凹。     

用mtDNA 12S rRNA序列变异检验鲤形目鱼类系统发育关系

通过对鲤形目鱼类5个科的代表类群的完整线粒体12S rRNA进行测序和分析,以检验目前的形态学假说。经序列比对后,有1000个位点,其中467个位点在茎区,533个在环区;有395个位点为变化位点,其中267个为系统发育信息位点。采用邻接法和最大简约法进行了系统发育分析,其结果支持鲤科鱼类成为一个单系群,非鲤科的鲤形目鱼类成为另一个单系群的观点,这与Siebert提出的假说相一致。鲤科鱼类包含3个主要的分支,即鱼丹系、鲤系和雅罗鱼系;但在非鲤科鲤形目鱼类中,其关系不能得到很好的解决,其中鳅科是复系,平鳍鳅科、条鳅亚科和花鳅亚科可能有较近的亲缘关系。     

Threatened fishes of the world: Cyprinus micristius Regan 1906 (Cypriniformes: Cyprinidae)

Conservation status, identification, distribution, abundance, habitat and ecology, conservation actions and recommendations of a endemic cyprinid fish, Cyprinus micristius were introduced based on data and knowledge from a GEF project in Lake Dianchi, Yunnan, China.     

基于Rag1基因序列变异的鲤形目鱼类系统发育重建:采样策略对生命之树的影响

The phylogenetic relationships of species are fundamental to any biological investigation, including all evolutionary studies. Accurate inferences of sister group relationships provide the researcher with an historical framework within which the attributes or geographic origin of species (or supraspecific groups) evolved. Taken out of this phylogenetic context, interpretations of evolutionary processes or origins, geographic distributions, or speciation rates and mechanisms, are subject to nothing less than a biological experiment without controls. Cypriniformes is the most diverse clade of freshwater fishes with estimates of diversity of nearly 3,500 species. These fishes display an amazing array of morphological, ecological, behavioral, and geographic diversity and offer a tremendous opportunity to enhance our understanding of the biotic and abiotic factors associated with diversification and adaptation to environments. Given the nearly global distribution of these fishes, they serve as an important model group for a plethora of biological investigations, including indicator species for future climatic changes. The occurrence of the zebrafish, Danio rerio, in this order makes this clade a critical component in understanding and predicting the relationship between mutagenesis and phenotypic expressions in vertebrates, including humans. With the tremendous diversity in Cypriniformes, our understanding of their phylogenetic relationships has not proceeded at an acceptable rate, despite a plethora of morphological and more recent molecular studies. Most studies are pre-Hennigian in origin or include relatively small numbers of taxa. Given that analyses of small numbers of taxa for molecular characters can be compromised by peculiarities of long-branch attraction and nodal-density effect, it is critical that significant progress in our understanding of the relationships of these important fishes occurs with increasing sampling of species to mitigate these potential problems. The recent Cypriniformes Tree of Life initiative is an effort to achieve this goal with morphological and molecular (mitochondrial and nuclear) data. In this early synthesis of our understanding of the phylogenetic relationships of these fishes, all types of data have contributed historically to improving our understanding, but not all analyses are complementary in taxon sampling, thus precluding direct understanding of the impact of taxon sampling on achieving accurate phylogenetic inferences. However, recent molecular studies do provide some insight and in some instances taxon sampling can be implicated as a variable that can influence sister group relationships. Other instances may also exist but without inclusion of more taxa for both mitochondrial and nuclear genes, one cannot distinguish between inferences being dictated by taxon sampling or the origins of the molecular data.     

西昌白鱼一新亚种描述及其亚种分化(鲤形目,鲤科)

1996年11月至1997年6月,于雅砻江下游干流及其支流鳡鱼河中采获一批白鱼属Anabarilius鱼类标本,经鉴定为西昌白色1新亚种。命名为西昌白色雅砻亚种Anabarilius liui yalongensis subsp.nov。与其它3个亚种的主要鉴别特征是：第1鳃弓外侧鳃耙为7—10,其余3个亚种分别为：西昌指名亚种A.liui liui为12-14,程海亚种A．liui chenghaiensis 15-16,宜良亚种A．liui yiliangensis10-12。种内亚种分化主要是受到同域生态隔离和地理隔离作用的影响。     

中国爬岩鳅属鱼类一新种记述(鲤形目,平鳍鳅科)

记述了采自云南省沾益县德泽乡牛栏江水域的平鳍鳅科爬岩鳅属鱼类1新种,牛栏爬岩鳅 Beaufortia niulanensis Chen,Huang et Yang,sp.nov..其特征:背鳍分支鳍条7,腹鳍分支鳍条21,侧线鳞90～95,胸鳍起点相对于鼻孔和眼前缘之间,背鳍起点相对于腹鳍起点至其基部后缘的中点稍后,肛门位于腹鳍基后缘至臀鳍起点间的中点,腹鳍末端接近肛门,这些特征组合可以将新种与同属的其他2个相近种,即四川爬岩鳅B.szechuanensis(Fang)和中间爬岩鳅B.intermedia Tang et Wang区别开来.     

中国广西金线鲃属一新种(鲤形目,鲤科,鲃亚科)

2001年5月作者于广西环江县驯乐乡顺宁村采集到金线鲃属盲鱼5尾,经鉴定为一新种.新种眼睛完全退化,头后急剧隆起但尚未形成额骨突等体型特征与无眼金线鲃Sinocyclocheilus anophthalmus Chen et Chu,1988相似,而明显有别于属内其他物种.但两者在以下性状上又存在明显区别:1)新种背鳍硬刺纤弱;而无眼金线鲃的背鳍硬刺比较粗壮;2)新种背部自额骨后隆起,但是额骨顶部并不隆起;无眼金线鲃则自眼眶上方的额骨顶部开始显著隆起,直至肩部;3)新种侧线平直;无眼金线鲃的侧线在腹鳍和臀鳍之间的上方有一比较明显的向上弯曲;4)新种胸鳍长,超过腹鳍起点;无眼金线鲃胸鳍不超过腹鳍起点;5)分布区亦有所不同,新种分布于柳江水系;无眼金线鲃则分布于南盘江水系.此外,在一些可测性状上二者亦有区别,在标准长与体高和头长的比例、尾柄长与尾柄高的比例、侧线鳞的数目等性状上都有所差异.     

鳅鮀属鱼类一新种(鲤形目:鲤科)

本文记述采自江西的鲤科鱼类一新种。新种命名为江西鳅Ｇｏｂｉｏｂｏｔｉａｊｉａｎｇｘｉｅｎｓｉｓ,ｓｐ．ｎｏｖ,以胸腹部裸露区止于肛门;须短小;胸鳍条不延长,不达腹鳍起点;腹鳍起点至胸鳍起点距离大于至臀鳍起点距离;肛门位于腹鳍起点和臀鳍起点的中点等特征与本属其它种类相区别。