Modification de la fertilité du carpocapse Cydia pomonella à la suite du contact des adultes avec un régulateur ou un inhibiteur de croissance d'insectes

Résumé Des papillons de carpocapse Cydia pomonella L., mâles et femelles séparés, sont soumis au contact des résidus secs du CGA 184'699, un inhibiteur de croissance d'insectes (ICI) et du fenoxycarb, un régulateur de croissance d'insectes (RCI). Ils sont ensuite regroupés dans boîtes non traitées pour l'accouplement et la ponte. La fécondité du carpocapse n'est pratiquement pas affectée par ce conditionnement mais sa fertilité diminue avec l'augmentation de la concentration des produits et avec la durée d'exposition. Avec le CGA 184'699, l'efficacité ovicide induite atteint environ 50% suite à un contact de trois jours avec les résidus d'un traitement à 100 mg/l, alors qu'avec le fenoxycarb elle s'élève à 87% dans les mêmes conditions. Avec le CGA 184'699, seul le contact des femelles avec les résidus provoque cette réduction de fertilité, alors que le fenoxycarb agit sur les deux sexes.

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Summary Codling moth, Cydia pomonella L., males and females were exposed separately to contact with dry residues of CGA 184'699 an insect growth inhibitor (IGI) and fenoxycarb, an insect growth regulator (IGR), The sexes were then grouped together in non treated boxes for mating and egglaying. Fecundity of codling moth was little affected by this treatment but its fertility decreased with increases in the concentration of products and the duration of contact. Induced ovicidal efficacy was about 50% after three days contact with the residues of a 100 mg (a.i.)/l application of CGA 184'699, whereas the same conditions led to about 87% efficacy with fenoxycarb. With CGA 184'699, only contact by females affected egg eclosion whereas fenoxycarb acted on both sexes.

     

Key elements in the successful control of diapausing codling moth,Cydia pomonella (Lepidoptera: Tortricidae) in wooden fruit bins with a South African isolate of Heterorhabditis zealandica (Rhabditida: Heterorhabditidae)

The non-insecticidal control strategies currently being implemented in South African orchards for the control of codling moth, Cydia pomonella (L.) may be hampered by wooden fruit bins being infested with diapausing codling moth larvae, acting as a potential source of re-infestation. Key factors contributing to the success or failure of an entomopathogenic nematode application were investigated using the SF 41 isolate of Heterorhabditis zealandica in laboratory bioassays with wooden minibins. Under operational conditions, an application rate of 100 IJs/mL (LD₉₀₌102 IJs/mL) effectively controlled codling moth larvae in these bins, and for further laboratory bioassays, the LD₅₀ value of 18 IJs/mL (?25 IJs/mL) was identified as the discriminating dosage. Maximum mortality was attained when bins were pre-wet for at least 1 min (>90% RH) and maintained at maximum humidity (>95% RH) post-treatment for at least 3 days (LT₉₀=73 h), to ensure nematode survival and subsequent satisfactory infection of diapausing codling moth larvae. Tarping bins achieved the desired high level of humidity required. Furthermore, adjuvants (specifically Reverseal 10?) also improved an application. The study conclusively illustrated that if all the above-mentioned conditions are met, H. zealandica has the potential to successfully disinfest wooden fruit bins of codling moth.     

Sex linkage of CpGV resistance in a heterogeneous field strain of the codling moth Cydia pomonella (L.)

The occurrence of codling moth populations in European apple orchards that were not controlled by Cydia pomonella granulovirus (CpGV) is the first reported case of field resistance against a baculovirus control agent. A monogenic dominant sex-linked mode of inheritance was previously demonstrated in single-pair crosses between a homogeneous resistant (CpRR1) and a susceptible (CpS) laboratory strain of codling moth. However, resistant field populations (CpR) are more heterogeneous in their levels of resistance, and the possibility that they could harbor different resistance genes to CpRR1 had not been directly addressed. Here we report single pair crossing experiments using a resistant codling moth strain collected from an apple orchard in the southwest of Germany. Single-pair crosses within the field strain revealed a genetic basis to the heterogeneity of CpR concerning CpGV resistance. Hybrid crosses to a susceptible laboratory strain and backcrosses of the F1 generation to the resistant CpR strain confirmed that the homogeneous CpRR1 and the heterogeneous field strain CpR share the same mode of inheritance. Thus the variable levels of CpGV resistance in field populations is likely due to frequency differences of the same resistance-conferring gene, rather than different genes, which will facilitate future efforts to monitor and manage resistance.     

Female sex pheromone of the Chinese tortrix Cydia trasias

The Chinese tortrix, Cydia trasias (Meyrick) (Lepidoptera: Tortricidae), is a major pest of the Chinese scholar-tree (Japanese pagoda tree), Sophora japonica L. Cydia trasias has two or three generations in Beijing, China and overwinters as larvae in seed pods, bark crevices, and twigs of the Chinese scholar-tree (Chen, 1992; Chen & Qi, 1992). The larvae attack both petioles and seed pods. During outbreaks, the larvae can cause extensive leaf drop, bare twigs, and seed pod damage, reducing the ornamental and economic value of the tree (Enda & Yamazaki, 1987; Chen, 1992). Since the Chinese scholar-tree is one of the most popular ornamental trees in urban and suburban areas, trees are sprayed with insecticide to control C. trasias outbreaks. Earlier field trials showed that C. trasias can be controlled by mating disruption (Zhang et al., 2001). In our early paper and patent (Fu & Meng, 1997; Meng et al., 1998), we reported E8,E10-dodecadienyl acetate (E8,E10-12:Ac) as a pheromone component of C. trasias, but the identification of C. trasias sex pheromone was not complete. We report here the identification and field testing of the female-produced sex pheromone in C. trasias.