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31.
A study was made of the sterol glucosylating ability of cell-free homogenates obtained from 16 species of photosynthesizing and nonphotosynthesizing lower plants (2 species of Chlorophyceae, 2 species of Cyanophyceae, 1 species of Phycomycetes, 3 species of Ascomycetes, 3 species of Basidomycetes, 1 species of Myxomycetes, 3 species of Musci and 1 species of Sphenopsida). Except for the blue-green and green algae, all the remaining species showed distinct in vitro synthesis of steryl monoglucosides from UDPG and cholesterol or sitosterol. Preliminary studies on the specificity of the relevant enzymes pointed to a correlation between the sterol composition of the plant and the specificity of its glucosylating enzyme. The enzyme from Ascomycetes and Basidomycetes utilized ergosterol better than cholesterol or sitosterol. Enzymic preparations from mosses utilized sitosterol the best. 相似文献
32.
Sayaka?Morita Syuntaro?HiradateEmail author Yoshiharu?Fujii Jiro?Harada 《Plant Growth Regulation》2005,46(2):125-131
Crude extracts of the leaves of Spiraea prunifolia Sieb. showed high plant-growth-inhibiting activity comparable to that of S. thunbergii extracts. To isolate the causal compound in S. prunifolia, we performed bioassay-directed purification by monitoring the biological activity per unit weight of the organism containing the bioactive compound (total activity). We isolated 1-O-cis-cinnamoyl-β-D-glucopyranose (cis-CG) and identified it as the most important growth-inhibiting constituent in the crude extracts. We did not detect 6-O-(4′-hydroxy-2′-methylenebutyroyl)-1-O-cis-cinnamoyl-β-D-glucopyranose (cis-BCG) in S. prunifolia, though it is a major plant growth inhibitor in S. thunbergii together with cis-CG. We estimated the cis-CG content in S. prunifolia to be 3.84 mmol kg−1 F.W. This amount is comparable to the cis-CG plus cis-BCG content in S. thunbergii (3.59 mmol kg−1 F.W.). This indicates that S. prunifolia and S. thunbergii have equally high potential to inhibit plant growth, and cis-CG acts as the most important plant-growth inhibitor in S. prunifolia extracts. 相似文献
33.
Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l−1. 相似文献
34.
Seigler DS 《Phytochemistry》2005,66(13):1567-1580
The major cyanogenic glycoside of Guazuma ulmifolia (Sterculiaceae) is (2R)-taxiphyllin (>90%), which co-occurs with (2S)-dhurrin. Few individuals of this species, but occasional other members of the family, have been reported to be cyanogenic. To date, cyanogenic compounds have not been characterized from the Sterculiaceae. The cyanogenic glycosides of Ostrya virginiana (Betulaceae) are (2S)-dhurrin and (2R)-taxiphyllin in an approximate 2:1 ratio. This marks the first report of the identification of cyanogenic compounds from the Betulaceae. Based on NMR spectroscopic and TLC data, the major cyanogenic glucoside of Tiquilia plicata is dhurrin, whereas the major cyanide-releasing compound of Tiquilia canescens is the nitrile glucoside, menisdaurin. NMR and TLC data indicate that both compounds are present in each of these species. The spectrum was examined by CI-MS, 1H and 13C NMR, COSY, 1D selective TOCSY, NOESY, and 1J/2,3J HETCOR experiments; all carbons and protons are assigned. The probable absolute configuration of (2R)-dhurrin is established by an X-ray crystal structure. The 1H NMR spectrum of menisdaurin is more complex than might be anticipated, containing a planar conjugated system in which most elements are coupled to several other atoms in the molecule. The coupling of one vinyl proton to the protons on the opposite side of the ring involves a 6J- and a 5/7J-coupling pathway. A biogenetic pathway for the origin of nitrile glucosides is proposed. 相似文献
35.
36.
The levels of cyanogenic glucosides (linamarin and lotaustralin) and the activity of linamarase were studied in 5-day old
seedlings of oil flax (Linum usitatissimum L., cv. LCSD 200) under different environmental conditions. White light enhanced the cyanoglucosides content, and this effect
depended on its intensity and the time of exposure. The level of cyanoglucosides rose with temperature, and it reached the
highest level at the highest temperature (30 °C).
Linamarase (EC. 3.2.1.21) activity was the highest at 20°C, especially in light-grown seedlings. Lower enzyme activity at
the extreme temperature (15 and 30 °C) was observed.
Water stress (low water potential, ω=−0.34 MPa) reduced by more than twice the cyanoglucoside level and linamarase activity.
The possible protective, or/and regulatory roles of cyanogenic glucosides was discussed. 相似文献
37.
Phylogenetic relationships within Plantago (Plantaginaceae): evidence from nuclear ribosomal ITS and plastid trnL-F sequence data 总被引:1,自引:0,他引:1
NINA RØNSTED MARK W. CHASE DIRK C. ALBACH MARIA ANGELICA BELLO 《Botanical journal of the Linnean Society. Linnean Society of London》2002,139(4):323-338
A molecular phylogenetic study of Plantago L. (Plantaginaceae) analysed nucleotide variation in the internal transcribed spacers (ITS) of nuclear ribosomal and plastid trnL-F regions. Included are 57 Plantago species, with two Aragoa species as the ingroup and three Veronica species as the outgroup. Phylogenetic analysis using maximum parsimony identified five major clades, corresponding to the taxonomic groups Plantago subgenera Plantago, Coronopus, Psyllium, Littorella and Bougueria . Aragoa is sister to genus Plantago . Plantago subgenus Littorella is sister to the other subgenera of Plantago . The results are in general correlated with a morphological phylogenetic study and iridoid glucoside patterns, but Plantago subgenus Albicans is paraphyletic and should be included in Plantago subgenus Psyllium sensu lato to obtain a monophyletic clade with six sections. Plantago section Hymenopsyllium is more closely related to section Gnaphaloides than to section Albicans . Plantago subgenus Bougueria is sister to subgenus Psyllium s.l. section Coronopus in Plantago subgenus Coronopus is subdivided in two series. Only some of the sections can be resolved into series. DNA variation within genus Plantago is high, a result that would not have been predicted on the basis of morphology, which is relatively stereotyped. If we calibrate a molecular clock based on the divergence of P. stauntoni , endemic to New Amsterdam in the southern Indian Ocean, we calculate the time of the split between Plantago and Aragoa to be 7.1 million years ago, which is congruent with the fossil record. © 2002 The Linnean Society of London, Botanical Journal of the Linnean Society , 2002, 139 , 323–338. 相似文献
38.
Won S. Woo 《Phytochemistry》1974,13(12):2887-2889
39.
40.
D.S. Seigler 《Phytochemistry》1975,14(1):9-29
The literature dealing with the detection, isolation, purification and characterization of cyanogenic glycosides has been integrated with spectral and chemical data as well as other techniques from our laboratory to establish a method for the positive identification of glycosides of this type. The compounds are arranged into biosynthetically related groups (those derived from l-phenylalanine; l-tyrosine; l-leucine, l-valine; l-isoleucine; those with cyclopentene rings and pseudocyanogenic glycosides) and features of each of the above procedures are critically reviewed and spectral data for each group presented (IR, MS, UV and NMR). The NMR spectra of TMS ethers of cyanogenic glycosides have proven especially useful in chemical structure determination. This information is sufficient to permit identification of any of the 26 known glycosides as well as certain uncharacterized ones. 相似文献