全文获取类型
收费全文 | 4333篇 |
免费 | 112篇 |
国内免费 | 25篇 |
专业分类
4470篇 |
出版年
2023年 | 25篇 |
2022年 | 34篇 |
2021年 | 41篇 |
2020年 | 62篇 |
2019年 | 63篇 |
2018年 | 74篇 |
2017年 | 40篇 |
2016年 | 66篇 |
2015年 | 54篇 |
2014年 | 85篇 |
2013年 | 225篇 |
2012年 | 61篇 |
2011年 | 361篇 |
2010年 | 274篇 |
2009年 | 368篇 |
2008年 | 297篇 |
2007年 | 285篇 |
2006年 | 282篇 |
2005年 | 271篇 |
2004年 | 308篇 |
2003年 | 99篇 |
2002年 | 170篇 |
2001年 | 58篇 |
2000年 | 51篇 |
1999年 | 66篇 |
1998年 | 133篇 |
1997年 | 49篇 |
1996年 | 74篇 |
1995年 | 146篇 |
1994年 | 66篇 |
1993年 | 17篇 |
1992年 | 34篇 |
1991年 | 15篇 |
1990年 | 22篇 |
1989年 | 16篇 |
1988年 | 14篇 |
1987年 | 17篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1984年 | 17篇 |
1983年 | 16篇 |
1982年 | 22篇 |
1981年 | 9篇 |
1980年 | 14篇 |
1979年 | 15篇 |
1978年 | 10篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 5篇 |
1974年 | 3篇 |
排序方式: 共有4470条查询结果,搜索用时 10 毫秒
991.
Florian Biba Michael Groessl Alexander Egger Michael A. Jakupec Bernhard K. Keppler 《化学与生物多样性》2009,6(12):2153-2165
The lanthanide complex aquatrichloridobis(1,10‐phenanthroline)cerium(III) [Ce(phen)2(H2O)Cl3] (KP776) was fully characterized by elemental analysis, IR‐, and 1H‐ and 13C‐NMR spectroscopy, as well as TG/DTA measurements, and its behavior in H2O, important for the application as a chemotherapeutic, was studied. In addition, the binding of KP776 to nucleotides and single serum proteins was investigated by capillary electrophoresis, whereas binding to proteins in human plasma was observed by ICP‐MS. The compound shows promising anticancer properties in vitro: proliferation of human cancer cell lines is strongly inhibited with IC50 values in the very low micromolar range. 相似文献
992.
Kaspar Hegetschweiler Michael Wrle Marc D. Meienberger Reinhard Nesper Helmut W. Schmalle Robert D. Hancock 《Inorganica chimica acta》1996,250(1-2):35-47
The interaction of 1,3,5-triamino-1,3,5-trideoxy-cis-inositol (taci) and its N-methylated derivative 1,3,5-trideoxy-1,3,5-tris(dimethylamino)-cis-inositol (tdci) with the incomplete [Mo3S4]4+ cube and the heterometallic [Mo3S4Cu]4+ cube have been investigated by X-ray analysis. The crystal structures of [Mo3S4(taci+ rmC3H6O-H2O)3-4H]·2OH2O (1a, rhombohedral, space group R32, A = 15.964(3), C = 40.59(1) Å, Z = 6), [Mo3S4(tdci)3]Br4·9.5EtOH·5H2O (2a, triclinic, space group and [CuBrMo3S4(tdci)3]Br3·11 H2O·EtOH (3a, monoclinic, space group P2,/n, A = 14.887(3), B = 22.570(4), C = 21.974(5) Å, β = 98.54(2)°, Z = 4) revealed andN-N-O and an N-O-O coordination mode for taci and tdci, respectively. In 1a, taci is coordinated as an anion with deprotonated oxygen and nitrogen donors. In addition, the non-coordinating amino group reacted with one equivalent; of acetone, forming a Schiff base condensation product. For 2a, short Mo---O bonds and high pKa values (compared to the aqua ion [Mo3S4(H2O)9]4+) indicate the formation of a zwitterionic form of the tdci ligand with coordinated alkoxo groups and peripheral dimethylammonium groups. No significant differences were found for the structural properties of the Mo-tdci fragment in 2a and 3a. The coordination modes of taci and tdci, as observed in the solid state, are in agreement with the previously reported solution structures, established by NMR spectroscopy. They are attributed to the specific steric requirements of the two ligands and to a pronounced preference of the [Mo3(μS)3(μ3S)]4+ core to coordinate a nitrogen donor trans to μ3S. 相似文献
993.
《Molecular & cellular proteomics : MCP》2023,22(1):100481
Meiotic maturation is an intricate and precisely regulated process orchestrated by various pathways and numerous proteins. However, little is known about the proteome landscape during oocytes maturation. Here, we obtained the temporal proteomic profiles of mouse oocytes during in vivo maturation. We successfully quantified 4694 proteins from 4500 oocytes in three key stages (germinal vesicle, germinal vesicle breakdown, and metaphase II). In particular, we discovered the novel proteomic features during oocyte maturation, such as the active Skp1–Cullin–Fbox pathway and an increase in mRNA decay–related proteins. Using functional approaches, we further identified the key factors controlling the histone acetylation state in oocytes and the vital proteins modulating meiotic cell cycle. Taken together, our data serve as a broad resource on the dynamics occurring in oocyte proteome and provide important knowledge to better understand the molecular mechanisms during germ cell development. 相似文献
994.
Julio J. Criado M.C. Garcia-Moreno Rocio R. Macias Jose J.G. Marin Manuel Medarde E. Rodriguez-Fernandez 《Biometals》1999,12(3):283-290
With a view to using bile acids as shuttles for delivering platinum-related cytostatic drugs to liver tumors, a chenodeoxycholylglycinato(CDCG)-derivative of platinum(II) has been synthesized. The complex - named Bamet-M2- was chemically characterized by elemental analysis, FT-IR, NMR, FAB-MS, and UV spectroscopy. The results indicate the following composition: C26H42N2O5Cl2NaPt(II), the metal Pt(II) being bound to two Cl– and one bidentate CDCG moiety, i.e., Na[Pt CDCG(N,O) Cl2]. The compound is highly soluble (up to 20 mM) in water and (up to 35 mM) in ethanol, methanol and DMSO. Hydrolysis was investigated because this is assumed to be an important step in intracellular activation and interaction with DNA of this type of compounds. The reaction kinetics of this complex in aqueous solution show unusual behaviour; the substitution process with the displacement of two Cl– was almost instantaneous, and the resulting species were found to be very stable. Kinetic studies carried out in the presence of different NaCl concentrations (up to 500 mM) revealed similar fast and nonreversible aquations of Bamet-M2. This contrasts with the slow aquation of cisplatin in extracellular-line solutions (i.e., at high NaCl concentrations) as compared with fast hydrolysis in cells. This difference may partly account for the low cytostatic activity observed here for Bamet-M2 against several tumor cell-lines. 相似文献
995.
The photosynthetic apparatus of Rhodopseudomonas palustris contains, in addition to reaction center bacteriochlorophyll (Bchl) two spectral forms of light harvesting (LH) Bchl, i.e. LH Bchl I, characterized by an infrared absorption maximum at 880 nm (890 nm at 77°K) and LH Bchl II absorbing at 805 and 855 nm (805 and 870 nm at 77°K). LH Bchl I seems to be associated with a single protein species of an apparent mol. wt. of 13000 whereas LH Bchl II is apparently associated with two proteins of mol. wts. of 9000 and 11000.Cells in anaerobic cultures adapt to changes of light intensity 1. by variation of the size of the photosynthetic unit, i.e. the molar ratio of LH Bchl II to reaction center Bchl, 2. by variation of the number of photosynthetic units per unit of membrane area, 3. by regulation of the size of the intracytoplasmic membrane system.During adaptation of changes of oxygen partial pressure cells are able to synthesize reaction center Bchl, LH Bchl and intracytoplasmic membranes at different rates. The synthesis of reaction center Bchl and LH Bchl I are, however, coordinated with each other, while the syntheses of LH Bchl II and reaction center Bchl proceed independently.List of Non-Standard Abbreviations Bchl
bacteriochlorophyll
- ICM
mitracytoplasmic membrane
- LDAO
lauryldimethyl aminoxide
- R
Rhodopseudomonas
- RC
reaction center
- SDS
sodium dodecylsulfate 相似文献
996.
Susanne Homma Andrea Horsch Marie-Noëlle Pouch Franck Petit Yves Briand Hans-Peter Schmid 《Molecular biology reports》1994,20(2):57-61
Proteasomes (prosomes) are large multiprotein complexes. They are involved in protein degradation of ubiquitin-conjugated proteins and in the generation of MHC class I peptides. We gave further evidence that they interfere within vitro protein synthesis. Proteasomes inhibit the translation of Tobacco mosaic virus RNA. Analysis of cell-free systems by sucrose gradient centrifugation revealted that they prevent the formation of 80S initiation complexes but not the early phase of initiation. 相似文献
997.
The formation of ternary complexes, transferrin-anion-In111 has been investigated by means of gamma-gamma coincidence spectrometry of the 172-245 keV rays. The angular correlation between the two gamma-rays emitted in cascade depends on the magnetic and electric fields gradients, consequently the chemical structure of metal holder. Any modification of this structure causes the variation of angular correlation. The study of G22 (infinity) as function of pH (G22(infinity): integrated perturbed angular correlation coefficient) has been performed to turn out the hydrolysis of In111 in aqueous solution, metal complex formation in presence of chelating agents (citric acid and sodium bicarbonate) and the formation of protein-metal complexes. The presence of complexing agents limits the domain of In111 colloid existence and allows fast transfer of ionised indium on the transferrin. Two types of metal-protein interactions has been turn out. The first in the weakly acidic range of pH is characterized by an affinity constant near to this of citric acid. The second lying in neutral and basic range of pH, where the formation rate of transferrin-In111 complex is fast (t less than 500 s). In citrate medium, for pH 6-7,5 the rate of metal transfer on the protein, studied by means of G22 (infinity) = f(t), is function of pH. The binding anion appears as an indispensable element for the formation of protein-metal complexes. The In111 previously chelated by 8-Hydroxyquinoline is fixed by the protein if only exits a binding anion in the solution. This mays bring in the formation of an intermediate active state, indispensable step for the ternary complex formation transferrin-anion-In111. 相似文献
998.
S Báo W de Souza 《Biology of the cell / under the auspices of the European Cell Biology Organization》1992,75(1):37-44
A freeze-fracture study was carried out on spermatid and spermatozoon of the mosquito Culex quinquefasciatus. In the spermatid plasma membrane few and randomly distributed intramembranous particles were observed. In the spermatozoon the density of intramembranous particles was higher on the P- than on the E-fracture face of the plasma membrane. Two populations of particles were observed. Large particles (about 15 nm in diameter) are regularly arranged in double rows as a zipper-line, longitudinally oriented in relation to the main cell axis. These strands of particles were observed in the posterior head region, mainly associated with the E-fracture face. Filipin was used to analyse the presence and distribution of cholesterol in thin sections and freeze-fracture replicas. Filipin-sterol complexes were not homogeneously distributed throughout the spermatozoon plasma membrane. They were more abundant on the P-fracture face of the membrane lining the nuclear region. The results obtained show that Culex spermatozoon differs from those of other species in that its plasma membrane exhibits only a membrane domain, the zipper-line, localized in the postacrosomal region. 相似文献
999.
1000.
Radioactivity eventually destined for the chromatophore membrane of Rhodopseudomonas sphaeroides was shown in pulse-chase studies to appear first in a distinct pigmented fraction. This material formed an upper pigmented band which sedimented more slowly than chromatophores when cell-free extracts were subjected directly to rate-zone sedimentation on sucrose density gradients. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the purified fraction contained polypeptide bands of the same mobility as light-harvesting bacteriochlorophyll a and reaction center-associated protein components of chromatophores; these were superimposed upon cytoplasmic membrane polypeptides. The pulse-chase relation was confined mainly to the polypeptide components of these pigment-protein complexes. It is suggested that the isolated fraction may be derived from sites at which new membrane invagination is initiated. 相似文献