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81.
The metabolism of the herbicide, diclofop-methyl (methyl-2-[4-(2', 4'-dichlorophenoxy) phenoxy]propanoate), in cell suspension cultures of Avena sativa L. (cv. Garry) and in callus of Avena fatua L. (transferred to liquid) was determined as a function of time (8 h to about 3 weeks) and was compared to previous metabolism data from intact plants. A. fatua metabolized 14 C-labeled diclofop-methyl more rapidly than A. sativa, but the metabolites formed were similar if not identical. Within 2 days, approximately 50% of the total 14 C recovered was in A. fatua cells whereas less than 15% was in A. sativa cells. In older cultures of A. fatua, the amounts of 14 C in the cells and in the medium were about 45% each; 10 to 12% was in the non-extractable cell residue. The 14 C recovered from A. sativa cells increased to a maximum of about 35% at 7 days and then slowly decreased to about 18% by 21 days, whereas the 14 C in the medium of A. sativa decreased to about 60% at 7 days and then increased to over 75% by 21 days. The nonextractable 14 C residue was 5% or less even after 21 days. Major metabolites in methanolic extracts of cells of both A. sativa and A. fatua were diclofop (2-[4-(2', 4'-dichlorophenoxy)phenoxy] propanoate), diclofop hydroxylated at an undetermined position on the 2,4-dichlorophenyl ring (ring OH-diclofop), and conjugates of diclofop and ring-OH diclofop. 相似文献
82.
83.
Cleaved AFLP (cAFLP), a modified amplified fragment length polymorphism analysis for cotton 总被引:3,自引:0,他引:3
Zhang J Lu Y Yu S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1385-1395
In certain plant species including cotton (Gossypium hirsutum L. or Gossypium barbadense L.), the level of amplified fragment length polymorphism (AFLP) is relatively low, limiting its utilization in the development
of genome-wide linkage maps. We propose the use of frequent restriction enzymes in combination with AFLP to cleave the AFLP
fragments, called cleaved AFLP analysis (cAFLP). Using four Upland cotton genotypes (G. hirsutum) and three Pima cotton (G. barbadense), we demonstrated that cAFLP generated 67% and 132% more polymorphic markers than AFLP in Upland and Pima cotton, respectively.
This resulted in 15.5 and 25.5 polymorphic cAFLP markers per AFLP primer combination, as compared to 9.1 and 11.0 polymorphic
AFLP. The cAFLP-based genetic similarity (GS) is generally lower than the AFLP-based GS, even though both marker systems are
overall congruent. In some cases, cAFLP can better resolve genetic relationships between genotypes, rendering a higher discriminatory
power. Given the high-resolution power of capillary-based DNA sequencing system, we further propose that AFLP and cAFLP amplicons
from the same primer combination can be pooled as one sample before electrophoresis. The combination produced an average of
18.5 and 31.0 polymorphic markers per primer pair in Upland and Pima cotton, respectively. Using several restriction enzyme
combinations before pre-selective amplification in combination with various frequent 4 bp-cutters or 6 bp-cutters after selective
amplification, the pooled AFLP and cAFLP will provide unlimited number of polymorphic markers for genome-wide mapping and
fingerprinting. 相似文献
84.
本文先通过转移概率给出了刻划杂合状态起始细胞可纯化,单向纯化,双向纯化的特征,然后引入了临界平均后代数概念,并用此概念刻划了单向纯化、双向纯化的实质. 相似文献
85.
野生稻具有许多优良性状,是水稻遗传改良的重要物质基础。为了探讨应用原生质体融合技术转移野生稻有利基因的途径,进行了栽培用与4种野生稻融合试验。总计6·28×107个野生稻原生质体与栽培稻原生质体进行了电激融合。获得了4364块愈伤组织,再生了490个植株。试验了Y射线处理野生稻原生质体的有效剂量和碘代乙酰胺抑制栽培稻原生质体生长的浓度。探讨了原生质体来源对融合效果的影响,讨论了融合亲和性问题。 相似文献
86.
苹果的抗氰呼吸与果实呼吸跃变的关系 总被引:1,自引:0,他引:1
苹果在发育期间呼吸对KCN敏感,进入跃变期时呼吸为KCN不敏感,但对间氯苯氧肟酸(CLAM)敏感。跃变期的苹果切片陈化后有诱导呼吸发生,且KCN与CLAM对诱导呼吸都有一定的抑制作用。当在CLAM存在下进行陈化时,苹果诱导呼吸的发生被抑制约30%,跃变期果实的特点是当果实进入成熟期时呼吸途径发生了由细胞色素途径向抗氰的交替途经转移的现象;同时其果实切片在陈化后可以发生诱导呼吸。 相似文献
87.
88.
Maren Hellwig 《Vegetation History and Archaeobotany》1997,6(2):105-116
A variety of well-preserved plant remains was recovered from a pond and two cesspits from late medieval and post medieval Göttingen. Cultivated plants included cereals, oilseeds and fibre plants, vegetables, fruit, nuts and spices. Rice and spices were imported from India and Africa and point to the relative wealth of the users of one of the pits. Additionally, a number of wild fruit, includingSorbus torminalis (wild service), was gathered from woods, clearings or hedges. Gardens were situated in the town or around its walls. Hops and grapevines were grown in special gardens in favourable places out of town. Apart from human (and sometimes animal) faeces, mostly kitchen refuse and waste from cleaning grain and processing flax in the town were deposited in the pits. Thus weeds of arable land are well represented, some of them indicating mainly basic soil conditions on the cornfields. Short-lived as well as persistent ruderals found suitable growing conditions. Poor grasslands were grazed, those on more fertile soils were also used for haymaking. Swampy areas were exploited for litter. A number of the recorded plants, especially some arable weeds or ones needing damp conditions, are nowadays threatened or extinct in the region.This paper is dedicated to Ulrich Willerding on the occasion of his 65th birthday 相似文献
89.
Helmut Kroll 《Vegetation History and Archaeobotany》1997,6(1):25-67
Publications on archaeological remains of cultivated plants have been collected, mainly from 1995, with some earlier and some later ones. A list is given of the finds according to taxon, country, site, and age. 相似文献
90.
Liming Wang Yuping Tang William Eisner Matthew A. Sparks Anne F. Buckley Robert F. Spurney 《Biochemical and biophysical research communications》2014
To determine if augmenting podocyte injury promotes the development of advanced diabetic nephropathy (DN), we created mice that expressed the enzyme cytosine deaminase (CD) specifically in podocytes of diabetic Akita mice (Akita-CD mice). In these mice, treatment with the prodrug 5-flucytosine (5-FC) causes podocyte injury as a result of conversion to the toxic metabolite 5-fluorouracil (5-FU). We found that treatment of 4–5 week old Akita mice with 5-FC for 5 days caused robust albuminuria at 16 and 20 weeks of age compared to 5-FC treated Akita controls, which do not express CD (Akita CTLs). By 20 weeks of age, there was a significant increase in mesangial expansion in Akita-CD mice compared to Akita CTLs, which was associated with a variable increase in glomerular basement membrane (GBM) width and interstitial fibrosis. At 20 weeks of age, podocyte number was similarly reduced in both groups of Akita mice, and was inversely correlated with the albuminuria and mesangial expansion. Thus, enhancing podocyte injury early in the disease process promotes the development of prominent mesangial expansion, interstitial fibrosis, increased GBM thickness and robust albuminuria. These data suggest that podocytes play a key role in the development of advanced features of diabetic kidney disease. 相似文献