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101.
Bruce A. Robertson Richard L. Hutto Joseph J. Fontaine 《Journal of Field Ornithology》2010,81(4):420-429
ABSTRACT The use of aural surveys to estimate population parameters is widespread in avian studies. Despite efforts to increase the efficacy of this method, the potential for ecological context to bias population estimates remains largely unexplored. For example, food availability and nest predation risk can influence singing activity independent of density and, therefore, may bias aural estimates where these ecological factors vary systematically among habitats or other categories of ecological interest. We used a natural fire event in a mixed‐conifer forest that experienced variation in fire severity (low, intermediate, and high) to determine if aural surveys produce accurate density estimates of Dark‐eyed Juncos ( Junco hyemalis) independent of ecological context. During the first 2‐yr postfire, we censused junco populations in each burn type with intensive spot‐mapping and nest searching, locating 168 nests. Simultaneously, we conducted fixed‐radius point‐count surveys and estimated food availability and nest predation risk in each burn type to test whether ecological context may influence aural detection probability independent of actual density. We found no difference in nesting densities among patches burned at different severity. Arthropod food availability was inversely related to fire severity during the first postfire breeding season, but increased to higher levels across all severities during the second. In both years, aural detections were significantly greater in intermediate severity patches that consistently represented the habitat with the lowest nest predation risk. These results suggest that nest predation risk may significantly bias aural estimates of avian populations. Although traditional aural survey methods such as the Breeding Bird Survey measure habitat attributes, our findings highlight the difficulty in assessing relevant covariates in estimates of avian population. Future research must consider the potential for nest predation and other ecological factors to drive interannual or interhabitat variation in avian population estimates independent of true changes in population size. 相似文献
102.
103.
Deng B 《Journal of mathematical biology》1999,38(1):21-78
In the presence of stimulatory concentrations of glucose, the membrane potential of pancreatic β-cells may experience a transition
from periods of rapid spike-like oscillations alternating with a pseudo-steady state to spike-only oscillations. Insulin secretion
from β-cells closely correlates the periods of spike-like oscillations. The purpose of this paper is to study the mathematical
structure which underlines this transitional stage in a pancreatic β-cell model. It is demonstrated that the transition can
be chaotic but becomes more and more regular with increase in glucose. In particular, the system undergoes a reversed period-doubling
cascade leading to the spike-only oscillations as the glucose concentration crosses a threshold. The transition interval in
glucose concentration is estimated to be extremely small in terms of the rate of change for the calcium dynamics in the β-cells.
The methods are based on the theory of unimodal maps and the geometric and asymptotic theories of singular perturbations.
Received: 25 October 1996/Revised version: 18 August 1997 相似文献
104.
105.
Helguera-Repetto C Cox RA Muñoz-Sànchez JL Gonzalez-y-Merchand JA 《FEMS microbiology letters》2004,235(2):281-288
Although Mycobacterium marinum and Mycobacterium tuberculosis are very closely related they differ significantly in their growth rates. The Type strain of M. marinum and one clinical isolate were investigated and, like M. tuberculosis, were found to have a single rRNA (rrn) operon per genome located downstream from murA gene and controlled by two promoters. No sequence differences were found that account for the difference in the growth rates of the two species. We infer that M. tuberculosis has the capacity to synthesize rRNA much faster than it actually does; and propose that the high number of insertion sequences in this species attenuate growth rate to lower values. 相似文献
106.
Wilén J Hörnsten R Sandström M Bjerle P Wiklund U Stensson O Lyskov E Mild KH 《Bioelectromagnetics》2004,25(1):5-15
Operators of RF plastic sealers (RF operators) are an occupational category highly exposed to radiofrequency electromagnetic fields. The aim of the present study was to make an appropriate exposure assessment of RF welding and examine the health status of the operators. In total, 35 RF operators and 37 controls were included. The leakage fields (electric and magnetic field strength) were measured, as well as induced and contact current. Information about welding time and productivity was used to calculate time integrated exposure. A neurophysiological examination and 24 h ECG were also carried out. The participants also had to answer a questionnaire about subjective symptoms. The measurements showed that RF operators were exposed to rather intense electric and magnetic fields. The mean values of the calculated 6 min, spatially averaged E and H field strengths, in line with ICNIRP reference levels, are 107 V/m and 0.24 A/m, respectively. The maximum measured field strengths were 2 kV/m and 1.5 A/m, respectively. The induced current in ankles and wrists varied, depending on the work situation, with a mean value of 101 mA and a maximum measured value of 1 A. In total, 11 out of 46 measured RF plastic sealers exceeded the ICNIRP reference levels. RF operators, especially the ready made clothing workers had a slightly disturbed two-point discrimination ability compared to a control group. A nonsignificant difference between RF operators and controls was found in the prevalence of subjective symptoms, but the time integrated exposure parameters seem to be of importance to the prevalence of some subjective symptoms: fatigue, headaches, and warmth sensations in the hands. Further, RF operators had a significantly lower heart rate (24 h registration) and more episodes of bradycardia compared to controls. 相似文献
107.
Hsp42 is the general small heat shock protein in the cytosol of Saccharomyces cerevisiae 总被引:4,自引:0,他引:4 下载免费PDF全文
Haslbeck M Braun N Stromer T Richter B Model N Weinkauf S Buchner J 《The EMBO journal》2004,23(3):638-649
Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the unspecific aggregation of proteins. So far, Hsp26 was the only unambiguously identified member of the sHsp family in Saccharomyces cerevisiae. We show here that the sHsp system in the cytosol of S. cerevisiae consists of two proteins, Hsp26 and Hsp42. Hsp42 forms large dynamic oligomers with a barrel-like structure. In contrast to Hsp26, which functions predominantly at heat shock temperatures, Hsp42 is active as a chaperone under all conditions tested in vivo and in vitro. Under heat shock conditions, both Hsp42 and Hsp26 suppress the aggregation of one-third of the cytosolic proteins. This subset is about 90% overlapping for Hsp42 and Hsp26. The sHsp substrates belong to different biochemical pathways. This indicates a general protective function of sHsps for proteome stability in S. cerevisiae. Consistent with this observation, sHsp knockout strains show phenotypical defects. Taken together, our results define Hsp42 as an important player for protein homeostasis at physiological and under stress conditions. 相似文献
108.
Ice nucleation in nature: supercooling point (SCP) measurements and the role of heterogeneous nucleation 总被引:2,自引:0,他引:2
In biological systems, nucleation of ice from a supercooled aqueous solution is a stochastic process and always heterogeneous. The average time any solution may remain supercooled is determined only by the degree of supercooling and heterogeneous nucleation sites it encounters. Here we summarize the many and varied definitions of the so-called "supercooling point," also called the "temperature of crystallization" and the "nucleation temperature," and exhibit the natural, inherent width associated with this quantity. We describe a new method for accurate determination of the supercooling point, which takes into account the inherent statistical fluctuations of the value. We show further that many measurements on a single unchanging sample are required to make a statistically valid measure of the supercooling point. This raises an interesting difference in circumstances where such repeat measurements are inconvenient, or impossible, for example for live organism experiments. We also discuss the effect of solutes on this temperature of nucleation. Existing data appear to show that various solute species decrease the nucleation temperature somewhat more than the equivalent melting point depression. For non-ionic solutes the species appears not to be a significant factor whereas for ions the species does affect the level of decrease of the nucleation temperature. 相似文献
109.
Two approaches to determine the fraction (μ) of mitochondrial respiration sustained during illumination by measuring CO2 gas exchange are compared. In single leaves, the respiration rate in the light (`day respiration' rate Rd) is determined as the ordinate of the intersection point of A–ci curves at various photon flux densities and compared with the CO2 evolution rate in darkness (`night respiration' rate Rn). Alternatively, using leaves with varying values of CO2 compensation concentration (Γ), intracellular resistance (ri) and Rn, an average number for μ can be derived from the linear regression between Γ and the product riċRn. Both methods also result in a number c* for that intercellular CO2 concentration at which net CO2 uptake rate is equal to –Rd. c* is an approximate value of the photocompensation point Γ* (Γ in the absence of mitochondrial respiration), which is related
to the CO2/O2 specificity factor of Rubisco Sc/o. The presuppositions and limitations for application of both approaches are discussed. In leaves of Nicotiana tabacum, at 22 °C, single leaf measurements resulted in mean values of μ = 0.71 and c* = 34 μmol mol−1. At the photosynthetically active photon flux density of 960 μmol quanta m−2 s−1, nearly the same numbers were derived from the linear relationship between Γ and riċRn. c* and Rd determined by single leaf measurements varied between 31 and 41 μmol mol−1 and between 0.37 and 1.22 μmol m−2 s−1, respectively. A highly significant negative correlation between c* and Rd was found. From the regression equation we obtained estimates for Γ* (39 μmol mol−1), Sc/o (96.5 mol mol−1) and the mesophyll CO2 transfer resistance (7.0 mol−1 m2 s).
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
110.
Evidence for a pre-restriction point Cdk3 activity 总被引:4,自引:0,他引:4
We have examined the activity of cyclin-dependent kinase 3 (cdk3) during G1-phase of the cell cycle in Chinese Hamster Ovary (CHO) fibroblasts. Histone H1 kinase activity associated with anti-cdk3 immunoprecipitates peaked during a brief window of time, 2-3 h prior to the restriction point. In vitro cdk3 activity was sensitive to roscovitine, a drug previously shown to inhibit cdks 1, 2, and 5, but not cdk4 or 6. Early G1-phase activation of cdk3 was downregulated by treatment of cells with MG132, an inhibitor of the proteasome, and by the protein synthesis inhibitor cycloheximide. These results provide evidence for a pre-restriction point cdk3 activity that requires both the synthesis of a regulatory subunit and degradation of an inhibitor. 相似文献