Differentiation of Two New York Isolates of Pratylenchus penetrans Based on Their Reaction on Potato

The behavior of two isolates of Pratylenchus penetrans on six potato clones was assessed to test the hypothesis that these nematode isolates from New York were different. Four potato cultivars (Superior, Russet Burbank, Butte, and Hudson) and two breeding lines (NY85 and L118-2) were inoculated with nematode isolates designated Cornell (CR) and Long Island (LI). Population increase and egression of nematodes from roots were used to distinguish resistance and susceptibility of the potato clones. Based on numbers of eggs, juveniles, and adults in their roots 30 days after inoculation, potato clones Butte, Hudson, and L118-2 were designated resistant to the CR isolate and susceptible to the LI isolate. More eggs were found in the roots of all plants inoculated with the LI isolate than with the CR isolate. The clones NY85 and L118-2 were inoculated with the CR and LI isolates in a 2 x 2 factorial experiment to assess differences in nematode egression. Egression was measured, beginning 3 days after inoculation, for 12 days. The rates of egression were similar for the four treatments and fit linear regression models, but differences were detected in numbers of egressed nematodes. More nematodes of the CR isolate than the LI isolate egressed from L118-2. Differences in egression of females was particularly significant and can be used as an alternative or supplement to reproduction tests to assess resistance in potato to P. penetrans and to distinguish variation in virulence.     

冠状动脉内注射负荷量替罗非班对急性心肌梗死患者心肌灌注及心功能的影响

目的：探讨急性ST段抬高型心肌梗死（STEMI）患者急诊经皮冠状动脉注射负荷量盐酸替罗非班对心肌灌注及心功能的影响。方法：选择急性ST段抬高型心肌梗死患者270倒,随机分为实验组（n=133）和对照组（n=137）。所有入选患者均急诊进行经皮冠状动脉介入治疗（PCI）,术中实验组患者予冠脉内注射负荷量盐酸替罗非班,对照组不予注射,主要终点为观察PCI术后即刻造影结果,同时观察术后3天、30天、360天的左室射血分数（EF）,左室舒张末期内径（LVEDD）、左室收缩期末内径（LVESD）及术后360天主要心血管不良事件的发生率;次要终点为一般出血事件的发生率、血小板下降情况、血小板聚集率的变化。结果：实验组患者术后校正的TIMI帧数、术后2小时ST段回落程度、磷酸肌酸肌酶同工酶（CK-MB）峰值及峰值时间显著优于对照组（P〈0．05）．梗死相关血管无复流发生率及360天MACE的发生率显著低于对照组（P〈0．05）,与对照组相比,术后30天、360天的EF值明显增加,术后30天、360天的LVEDD、LVESD显著降低（P〈0．05）;实验组术后2天血小板聚集率明显低于对照组（P〈0．05）。结论：急诊PCI术中联合冠脉内注射负荷量替罗非班可以改善心肌灌注水平及心功能,且不增加出血事件的发生率,提高远期愈后。     

弹性超声成像特征与宫颈癌病理学特征的相关性研究

目的:探讨宫颈癌弹性超声成像特征与病理学特征的相关性。方法:选择2016年9月到2018年2月在我院接受治疗的50例宫颈癌(宫颈癌组)与190例癌前病变患者(癌前组)为研究对象,所有患者都给予彩色多普勒超声与弹性超声成像,记录超声特征;调查宫颈癌患者的病理学特征并进行相关性分析。结果:癌前组中超声血流分型Ⅰ型、Ⅱ型、Ⅲ型、Ⅳ型分别为160例、26例、4例与0例,宫颈癌组分别为0例、6例、20例、24例,两组超声血流分型分布比较差异有统计学意义(P0.05)。癌前组中弹性超声成像评分1分、2分、3分、4分、5分分别为52例、100例、33例、7例、0例,宫颈癌组分别为0例、2例、6例、12例与30例,两组中弹性超声成像评分分布对比差异有统计学意义(P0.05)。在宫颈癌患者中,随着淋巴结转移、分化程度降低、临床分期增加,弹性超声成像评分显著增加,差异有统计学意义(P0.05)。弹性超声成像诊断191例癌前病变与49例宫颈癌,弹性超声成像鉴别诊断宫颈癌的敏感性为100.0%,特异性为98.0%。结论:弹性超声成像诊断与鉴别宫颈癌有很好的敏感性与特异性,与宫颈癌病理学特征有很好的相关性,可与彩色多普勒超声形成互补在临床上应用。     

芪红汤联合美托洛尔对冠心病心绞痛气虚血瘀型患者血浆ET、hs-CRP、NT-proBNP水平的影响

目的:探讨芪红汤联合美托洛尔对冠心病心绞痛气虚血瘀型患者血浆内皮素(ET)、超敏C反应蛋白(hs-CRP)、氨基末端B型脑利钠肽原(NT-proBNP)水平的影响。方法:选择我院2014年6月~2016年6月收治的98例冠心病心绞痛气虚血瘀型患者,按治疗方式分为对照组与研究组,每组49例。对照组选用美托络尔治疗,研究组在美托络尔基础上联合芪红汤治疗,两组均持续治疗1个月。观察和比较两组的临床疗效,治疗前后症状积分、血浆ET、hs-CRP、NT-proBNP、左室射血分数(LVEF)、6 min步行试验、心绞痛持续时间、发作频率及心脏不良事件的发生情况。结果:治疗后,研究组总有效率显著高于对照组(P0.05),两组症状积分、ET、hs-CRP、NT-proBNP、心绞痛持续时间、发作频率均较治疗前显著降低(P0.05),且研究组以上指标均显著低于对照组(P0.05);两组LVEF、6-WMT均较治疗前上升(P0.05),且研究组以上指标均明显高于对照组(P0.05)。研究组心脏不良事件发生率显著低于对照组(P0.05)。结论:芪红汤联合美托络尔治疗冠心病心绞痛气虚血瘀型患者的临床效果肯定,能够有效降低患者血浆ET、hs-CRP、NT-proBNP水平。     

Safety and efficacy of a device to narrow the coronary sinus for the treatment of refractory angina: A single-centre real-world experience

Objective

The coronary sinus Reducer is a recently introduced device to treat patients with severe angina symptoms refractory to optimal medical therapy and not amenable for conventional revascularisation. We aimed to assess the safety and efficacy of the Reducer in a real-world cohort of patients with refractory angina.

Methods

This is a single-centre retrospective registry. Patients with severe angina symptoms, objective evidence of myocardial ischaemia using any adequate non-invasive modality and without options for conventional revascularisation were regarded eligible for Reducer implantation.

Results

Twenty-three patients (74?% male, mean age 70?±?8 years, 91.3?% previous bypass surgery, 82.6?% previous percutaneous intervention, 47.8?% previous myocardial infarction, 52.2?% diabetes mellitus) underwent Reducer implantation. The safety endpoint (successful implantation of the first device without device-related adverse events) was met in all patients. After a median follow-up of 9 (8–14) months the efficacy (any reduction in Canadian Cardiovascular Society (CCS) class and revascularisation-free survival) was reached in 17 patients (74?%): 8 patients (34.8?%) improved by 1 CCS class, 7 (30.4?%) by 2 CCS classes and 2 (8.7?%) by 3 CCS classes. One patient died 4 months after implantation because of progressive heart failure (not associated with Reducer implantation).

Conclusion

In this single-centre real-world experience, Reducer implantation was safe and demonstrated excellent clinical efficacy in the treatment of refractory angina at mid-term follow-up.

     

冠心病患者血清IL-37水平及其与血清IL-6、IL-10、CRP水平的关系

目的:探讨不同类型冠心病患者血清白介素-37(IL-37)的水平及其与血清白介素-6(IL-6)、白介素-10(IL-10)、C反应蛋白(CRP)水平的关系。方法:选取急性心肌梗死患者20例(AMI组)、不稳定性心绞痛患者26例(UAP组)、稳定性心绞痛患者20例(SAP组)及冠脉造影正常者26例(CON组)为研究对象,采用酶联免疫吸附法(ELISA)测定其血清IL-37、IL-6、IL-10和CRP的水平并分析其相关性。结果:1UAP组、AMI组血清IL-37水平均较对照组(CON组)显著增高(p0.05),而SAP组与CON组比较无明显差异(P0.05)。2冠心病患者的血清IL-37水平与其血清CRP(r=0.3,P0.05)、IL-6(r=0.4,P0.05)水平均存在显著正相关性,与IL-10水平无明显相关(P=0.16)。当排除SAP组患者后,冠心病患者的血清IL-37水平与CRP(r=0.3,P0.05)、IL-6(r=0.5,P0.05)、IL-10(r=0.2,P0.05)水平均显著相关。结论:急性冠脉综合症(ACS)患者的血清IL-37水平显著升高,并与IL-6、IL-10、CRP水平相关,可能参与了ACS发病过程中的炎症反应。     

胶原膜与羟基磷灰石应用于牙周牙髓联合病变的探讨

目的：初步探索胶原膜与羟基磷灰石在治疗牙周牙髓联合病变中牙槽骨缺损的作用。方法：患牙进行根管治疗,调颌及牙周翻瓣术。其中２１例患牙的牙周骨缺损区植入胶原膜和羟基磷灰石;２０例以传统血充盈法关闭骨腔。二组患牙于治疗后１、３、６、１２、２４个月分别进行随访;结果：提示应用胶原膜和羟基磷灰石组的疗效明显优于对照组,并有统计学上差异。结论：胶原膜加羟基磷灰石治疗牙周牙髓联合病变的方法值得提倡。     

DNA base repair – recognition and initiation of catalysis

Endogenous DNA damage induced by hydrolysis, reactive oxygen species and alkylation modifies DNA bases and the structure of the DNA duplex. Numerous mechanisms have evolved to protect cells from these deleterious effects. Base excision repair is the major pathway for removing base lesions. However, several mechanisms of direct base damage reversal, involving enzymes such as transferases, photolyases and oxidative demethylases, are specialized to remove certain types of photoproducts and alkylated bases. Mismatch excision repair corrects for misincorporation of bases by replicative DNA polymerases. The determination of the 3D structure and visualization of DNA repair proteins and their interactions with damaged DNA have considerably aided our understanding of the molecular basis for DNA base lesion repair and genome stability. Here, we review the structural biochemistry of base lesion recognition and initiation of one-step direct reversal (DR) of damage as well as the multistep pathways of base excision repair (BER), nucleotide incision repair (NIR) and mismatch repair (MMR).     

综合性心理干预对冠心病介入治疗患者抑郁焦虑及生活质量的影响

目的:探究综合性心理干预对冠心病介入治疗患者焦虑、抑郁及生活质量的影响。方法:选择2014年6月~2015年6月期间我院收治冠心病性介入治疗患者3280例为研究对象,采用随机数字法将其分为观察组(1648例)和对照组(1632例),观察组患者给予常规治疗、抗抑郁治疗及综合心理干预,对照组给予常规治疗、抗抑郁治疗;采用焦虑自评量表(SAS)、抑郁自评量表(SDS)评价患者治疗前后焦虑、抑郁状态,生活质量评价量表QLQ-C30量表评价患者治疗前后生活质量的变化情况。结果:两组患者在入院时SAS和SDS得分不存在差异(P0.05);干预1月后两组组患者的SAS和SDS得分均出现显著降低(P0.05),且观察组患者的SAS和SDS评分均明显低于对照组(P0.05);干预前两组患者生存质量各维度的评分均不存在显著差异(P0.05);干预1个月后两组患者躯体功能、角色功能、社会功能、情绪功能及总体症状较干预前均出现明显改善(P0.05),且干预后观察组患者躯体功能、角色功能、社会功能、情绪功能及总体症状均显著优于对照组(P0.05)。结论:冠心病介入治疗患者进行综合性心理干预能够改善患者心理状态,降低患者焦虑、抑郁情绪,提高生活质量,对临床冠心病的治疗有重要的意义。     

Reaction of Six Solanum tuberosum Cultivars to Pratylenchus penetrans

Six potato cultivars - Superior, Yukon Gold, Monona, Norchip, Kennebec, and Russet Burbank - were grown in Tioga loamy sand in tile microplots noninfested or infested with 9,800-11,500 P. penetrans/kg soil. At midseason, soil nematode population densities on Superior potatoes were 39,800/kg compared with 17,500-25,800/kg on the other cultivars. At harvest, 118 or 139 days after planting depending on maturity date, the final soil P. penetrans population density was 24,400/kg with Superior and 34,100-51,500/kg with the other cultivars. No differences occurred in the rate of nematode build-up in soil on the six potato cultivars. The nematode suppressed yield of marketable tubers of Superior by 73% and of Yukon Gold by 25%. Losses for Russet Burbank (61%), Kennebec (55%), Monona (46%), and Norchip (43%) were intermediate.