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961.
Compant S Kaplan H Sessitsch A Nowak J Ait Barka E Clément C 《FEMS microbiology ecology》2008,63(1):84-93
The colonization pattern of Vitis vinifera L. by Burkholderia phytofirmans strain PsJN was determined using grapevine fruiting cuttings with emphasis on putative inflorescence colonization under nonsterile conditions. Two-week-old rooted plants harbouring flower bud initials, grown in nonsterile soil, were inoculated with PsJN:gfp2x. Plant colonization was subsequently monitored at various times after inoculation with plate counts and epifluorescence and/or confocal microscopy. Strain PsJN was chronologically detected on the root surfaces, in the endorhiza, inside grape inflorescence stalks, not inside preflower buds and flowers but rather as an endophyte inside young berries. Data demonstrated low endophytic populations of strain PsJN in inflorescence organs, i.e. grape stalks and immature berries with inconsistency among plants for bacterial colonization of inflorescences. Nevertheless, endophytic colonization of inflorescences by strain PsJN was substantial for some plants. Microscopic analysis revealed PsJN as a thriving endophyte in inflorescence organs after the colonization process. Strain PsJN was visualized colonizing the root surface, entering the endorhiza and spreading to grape inflorescence stalks, pedicels and then to immature berries through xylem vessels. In parallel to these observations, a natural microbial communities was also detected on and inside plants, demonstrating the colonization of grapevine by strain PsJN in the presence of other microorganisms. 相似文献
962.
Targeted gene expression by the Gal4-UAS system in zebrafish 总被引:2,自引:0,他引:2
Targeted gene expression by the Gal4-UAS system is a powerful methodology for analyzing function of genes and cells in vivo and has been extensively used in genetic studies in Drosophila . On the other hand, the Gal4-UAS system had not been applied effectively to vertebrate systems for a long time mainly due to the lack of an efficient transgenesis method. Recently, a highly efficient transgenesis method using the medaka fish Tol2 transposable element was developed in zebrafish. Taking advantage of the Tol2 transposon system, we and other groups developed the Gal4 gene trap and enhancer trap methods and established various transgenic fish expressing Gal4 in specific cells. By crossing such Gal4 lines with transgenic fish lines harboring various reporter genes and effector genes downstream of UAS (upstream activating sequence), specific cells can be visualized and manipulated in vivo by targeted gene expression. Thus, the Gal4 gene trap and enhancer trap approaches together with various UAS lines should be important tools for investigating roles of genes and cells in vertebrates. 相似文献
963.
Information theoretical approaches to sensory processing in electric fish have focused on the encoding of amplitude modulations in a single sensory pathway in the South American gymnotiforms. To assess the generality of these studies, we investigated the encoding of amplitude and phase modulations in the distantly related African fish Gymnarchus. In both the amplitude- and time-coding pathways, primary afferents accurately estimated the time course of random modulations whereas hindbrain neurons extracted information about specific stimulus features. Despite exhibiting a clear preference for encoding amplitude or phase, afferents and hindbrain neurons could encode significant amounts of modulation of their nonpreferred attribute. Although no increase in feature extraction performance occurred where the two pathways converge in the midbrain, neurons there were increasingly sensitive to simultaneous modulation of both attributes. A shift from accurate stimulus estimation in the periphery to increasingly sparse representations of specific features appears to be a general strategy in electrosensory processing. 相似文献
964.
Distribution, morphology, and orientation of superficial neuromasts and polarization of the hair cells within superficial neuromasts of the goldfish (Carassius auratus) were examined using fluorescence labeling and scanning electron microscopy. On each body side, goldfish have 1,800-2,000 superficial neuromasts distributed across the head, trunk and tail fin. Each superficial neuromast had about 14-32 hair cells that were arranged in the sensory epithelium with the axis of best sensitivity aligned perpendicular to the long axis of the neuromast. Hair cell polarization was rostro-caudal in most superficial neuromasts on trunk scales (with the exception of those on the lateral line scales), or on the tail fin. On lateral line scales, the most frequent hair cell polarization was dorso-ventral in 45% and rostro-caudal in 20% of the superficial neuromasts. On individual trunk scales, superficial neuromasts were organized in rows which in most scales showed similar orientations with angle deviations smaller than 45 degrees . In about 16% of all trunk scales, groups of superficial neuromasts in the dorsal and ventral half of the scale were oriented orthogonal to each other. On the head, most superficial neuromasts were arranged in rows or groups of similar orientation with angle deviations smaller than 45 degrees . Neighboring groups of superficial neuromasts could differ with respect to their orientation. The most frequent hair cell polarization was dorso-ventral in front of the eyes and on the ventral mandible and rostro-caudal below the eye and on the operculum. 相似文献
965.
Plant communities affect the species–area relationship on Carex sempervirens tussocks 总被引:1,自引:1,他引:0
The species–area relationship (SAR) has been extensively studied in a wide range of plant communities, but very few studies have directly addressed how plant communities affect the SAR and what are the underlying mechanisms. Many graminoids form distinct tussocks where many other plant species grow, but no study has investigated whether the SAR holds true for the vegetation on tussocks. In four plant communities on an abandoned subalpine pasture in the Swiss National Park, we made releves on 600 tussocks of Carex sempervirens and measured tussock basal area and other tussock traits. In all four communities, species richness on C. sempervirens tussocks was strongly positively related to tussock basal area (R20.74), while other tussock traits explained very little (R2<0.04). Slope and intercept of the SAR on C. sempervirens tussocks differed significantly among the four communities. This was because plant communities affected richness in smaller tussocks (basal diameter <10 cm) but not that in large tussocks (basal diameter10 cm). We conclude that the SAR holds true for vegetation on C. sempervirens tussocks and changes with plant communities. Changes in the SAR on C. sempervirens tussocks are very likely because smaller tussocks are less independent of the plant communities than the larger ones, regarding disturbance or nutrients. 相似文献
966.
Ruth H. Leeney Rachel Amies Annette C. Broderick Matthew J. Witt Jan Loveridge Joana Doyle Brendan J. Godley 《Biodiversity and Conservation》2008,17(10):2323-2338
Marine vertebrate strandings data can provide insights into the long-term dynamics of cetacean populations, and the threats
they face. We investigate whether the spatio-temporal patterns of cetacean strandings around Cornwall, SW Britain, have changed
in the past century. Analysis of strandings from 1911 to 2006 (n = 2,257) show that, since the mid-1970s, the relative frequency of strandings of common dolphins (Delphinus delphis), harbour porpoises (Phocoena phocoena) and pilot whales (Globicephala melas) has increased significantly. Seasonal peaks in strandings frequencies are apparent, between December and March for harbour
porpoises and common dolphins, and between November and January for pilot whales. There were significant positive trends in
the number of common dolphin and harbour porpoise strandings, as a proportion of total strandings, over time. Strandings of
common dolphins, porpoises and all other species occur more frequently on the south coast of Cornwall. A total of 415 cetaceans
were subject to full veterinary necropsy to determine cause of death, between 1990 and 2006, and 253 (61%) of these individuals
were determined to have died due to bycatch in fishing gear. Analyses of industrialised fishing pressure in UK waters show
the seas around Cornwall to be one of the most heavily fished areas of the UK. We suggest a number of factors that could be
responsible for the recent increases in cetacean strandings in southwest UK waters in recent years, including survey effort,
as well as abundance and range shifts that are potentially linked with climate change. Although detectable levels of bycatch
rate have not increased over time, fisheries interactions are in significant part responsible for mortality patterns and are
worthy of more detailed investigation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
967.
Lydia C. L. Teh Louise S. L. Teh Fung Chen Chung 《Biodiversity and Conservation》2008,17(13):3061-3077
Many marine protected areas (MPAs), particularly in developing countries, have failed because of a lack of enforcement and
monitoring due to limited public funds for conservation. Private investment and management in MPAs offers a potential solution,
and has been applied with initial positive results at the Sugud Islands Marine Conservation Area (SIMCA) in Sabah, Malaysia.
Conservation fees charged to visitors to Lankayan Island Dive Resort within the SIMCA have generated a sustainable source
of financing to meet the majority of management costs for the conservation area, which is separately managed by a private
organization called Reef Guardian. The availability of adequate funds has enabled Reef Guardian to invest in personnel training
and surveillance technology to enforce the rules and regulations of the conservation area. In collaboration with government
enforcement agencies, Reef Guardian has reduced threats such as illegal fishing and turtle egg poaching. As a result, there
is a comparatively high abundance of commercially important fish, and turtle nestings at Lankayan Island have increased. Private
management can be effective in conserving biodiversity in MPAs, and may well succeed regionally in suitable locations.
相似文献
Lydia C. L. TehEmail: |
968.
Four microbial communities capable of degrading acetochlor, designated A, D, E, and J, were obtained from acetochlor-contaminated soil and sludge. Acetochlor at an initial concentration of 55mg/L was completely degraded by the four mixed cultures after 4 days. At 80 mg/L acetochlor, more than 99% degradation was observed with D, 84% with A and E, and 88% with J after 9 days. There are primary eight strains of bacteria in community A, three in community D, E, and J, respectively. No single isolate was able to degrade acetochlor efficiently. The acetochlor biodegradation products were identified by gas chromatography-mass spectrometry. The probable degradative pathways of acetochlor involved dechlorination, hydroxylation, deethoxymethylation, cyclization, carboxylation, and decarboxylation. Propachlor, alachlor, and metolachlor, which are also the main components of the chloroacetanilide herbicide, could be degraded by the four mixed cultures to some degree. Given the high degradation rates observed here, the four mixed cultures obtained may be useful in the degradation processes of acetochlor. 相似文献
969.
Facilitative interactions rather than resource partitioning drive diversity-functioning relationships in laboratory fungal communities 总被引:1,自引:1,他引:0
We manipulated the number of saprotrophic fungi in either a complex multi-resource substratum (sterilized forest soil), or a single-resource substratum (powdered cellulose). The substrates were inoculated with five common species of soil fungi in all possible combinations (from monocultures to five species in combination). In both substrates, the rate of organic matter decomposition was positively associated with species richness. The effect of fungal diversity was much stronger in the uniform single-resource substrate ( r 2 = 0.455, P < 0.0001) than in soil ( r 2 = 0.154, P < 0.0001). The results document that species richness of microbial decomposers strongly affects decomposition processes, at least at the species poor end of the diversity gradient. Both, 'sampling effect' and 'species complementarity effect' contributed to the community response with the latter being much more pronounced in uniform substrate than in soil. This indicates that facilitative interactions are more important than resource partitioning for positive effects of species richness. 相似文献
970.
As good as dead? Sublethal predation facilitates lethal predation on an intertidal clam 总被引:2,自引:0,他引:2
Although ecologists have speculated that sublethal predation can impact prey dynamics, consequences of these predator effects have seldom been experimentally tested. In soft‐sediment marine communities, fishes crop extended feeding siphons of buried clams, potentially causing clams to reduce their burial depth, thereby enhancing their susceptibility to excavating lethal predators. We simulated cropping of the confamilial clams, Protothaca staminea and Venerupis philippinarum, by removing the top 40% of siphons, which caused each species to burrow 33–50% shallower than conspecifics with intact siphons. To examine subsequent consequences of reduced burial depth, we exposed cropped and intact clams to natural levels of predation in the field. Because of a naturally longer siphon, Protothaca, even after cropping, remained at relatively safe burial depths. In contrast, siphon cropping nearly doubled the mortality rate of Venerupis. Thus, while sublethal predation facilitates lethal predation, this linkage depends on specific life history characteristics, even among ecologically similar species. 相似文献