全文获取类型
收费全文 | 761篇 |
免费 | 88篇 |
国内免费 | 26篇 |
出版年
2023年 | 9篇 |
2022年 | 11篇 |
2021年 | 17篇 |
2020年 | 25篇 |
2019年 | 23篇 |
2018年 | 29篇 |
2017年 | 27篇 |
2016年 | 28篇 |
2015年 | 26篇 |
2014年 | 38篇 |
2013年 | 51篇 |
2012年 | 20篇 |
2011年 | 37篇 |
2010年 | 14篇 |
2009年 | 46篇 |
2008年 | 35篇 |
2007年 | 50篇 |
2006年 | 36篇 |
2005年 | 42篇 |
2004年 | 45篇 |
2003年 | 36篇 |
2002年 | 21篇 |
2001年 | 22篇 |
2000年 | 19篇 |
1999年 | 25篇 |
1998年 | 10篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 10篇 |
1994年 | 13篇 |
1993年 | 6篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 16篇 |
1989年 | 6篇 |
1988年 | 8篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 2篇 |
排序方式: 共有875条查询结果,搜索用时 31 毫秒
71.
Ine Dorresteijn Jannik Schultner Dale G. Nimmo Joern Fischer Jan Hanspach Tobias Kuemmerle Laura Kehoe Euan G. Ritchie 《Proceedings. Biological sciences / The Royal Society》2015,282(1814)
Apex predators perform important functions that regulate ecosystems worldwide. However, little is known about how ecosystem regulation by predators is influenced by human activities. In particular, how important are top-down effects of predators relative to direct and indirect human-mediated bottom-up and top-down processes? Combining data on species'' occurrence from camera traps and hunting records, we aimed to quantify the relative effects of top-down and bottom-up processes in shaping predator and prey distributions in a human-dominated landscape in Transylvania, Romania. By global standards this system is diverse, including apex predators (brown bear and wolf), mesopredators (red fox) and large herbivores (roe and red deer). Humans and free-ranging dogs represent additional predators in the system. Using structural equation modelling, we found that apex predators suppress lower trophic levels, especially herbivores. However, direct and indirect top-down effects of humans affected the ecosystem more strongly, influencing species at all trophic levels. Our study highlights the need to explicitly embed humans and their influences within trophic cascade theory. This will greatly expand our understanding of species interactions in human-modified landscapes, which compose the majority of the Earth''s terrestrial surface. 相似文献
72.
The use of parallel labeling experiments for 13C metabolic flux analysis (13C-MFA) has emerged in recent years as the new gold standard in fluxomics. The methodology has been termed COMPLETE-MFA, short for complementary parallel labeling experiments technique for metabolic flux analysis. In this contribution, we have tested the limits of COMPLETE-MFA by demonstrating integrated analysis of 14 parallel labeling experiments with Escherichia coli. An effort on such a massive scale has never been attempted before. In addition to several widely used isotopic tracers such as [1,2-13C]glucose and mixtures of [1-13C]glucose and [U-13C]glucose, four novel tracers were applied in this study: [2,3-13C]glucose, [4,5,6-13C]glucose, [2,3,4,5,6-13C]glucose and a mixture of [1-13C]glucose and [4,5,6-13C]glucose. This allowed us for the first time to compare the performance of a large number of isotopic tracers. Overall, there was no single best tracer for the entire E. coli metabolic network model. Tracers that produced well-resolved fluxes in the upper part of metabolism (glycolysis and pentose phosphate pathways) showed poor performance for fluxes in the lower part of metabolism (TCA cycle and anaplerotic reactions), and vice versa. The best tracer for upper metabolism was 80% [1-13C]glucose+20% [U-13C]glucose, while [4,5,6-13C]glucose and [5-13C]glucose both produced optimal flux resolution in the lower part of metabolism. COMPLETE-MFA improved both flux precision and flux observability, i.e. more independent fluxes were resolved with smaller confidence intervals, especially exchange fluxes. Overall, this study demonstrates that COMPLETE-MFA is a powerful approach for improving flux measurements and that this methodology should be considered in future studies that require very high flux resolution. 相似文献
73.
Here we studied the occurrence of Cuckoo Cuculus canorus and top predators as indicators of biodiversity in agro ecosystems of Western Poland, to identify local hotspots. Bird species richness and land-use diversity were used as measures of biodiversity. The relationship between the presence of Cuckoos and four avian top predators with biodiversity measures were examined using Generalized Linear Mixed Models.Cuckoos were mainly distributed on sites with greater species richness, but were absent from the low species richness sites, while the top predators were distributed uniformly. The performance of the best models using the presence of Cuckoo was 27% higher than the best models using top predators. Our results highlight the predictive capacity of Cuckoos as an indicator of bird species richness than top predators and the usefulness of this species in biodiversity studies. The cookoo is charismatic, widespread across the main types of landscapes, and is easy to detect from its song. Importantly, our findings propose that cookoo can be used as effective and cheap tool to monitor the high bird diversity in different European countries. 相似文献
74.
Gatehouse AM Ferry N Edwards MG Bell HA 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2011,366(1569):1438-1452
With a projected population of 10 billion by 2050, an immediate priority for agriculture is to achieve increased crop yields in a sustainable and cost-effective way. The concept of using a transgenic approach was realized in the mid-1990s with the commercial introduction of genetically modified (GM) crops. By 2010, the global value of the seed alone was US $11.2 billion, with commercial biotech maize, soya bean grain and cotton valued at approximately US $150 billion. In recent years, it has become evident that insect-resistant crops expressing δ-endotoxin genes from Bacillus thuringiensis have made a significant beneficial impact on global agriculture, not least in terms of pest reduction and improved quality. However, because of the potential for pest populations to evolve resistance, and owing to lack of effective control of homopteran pests, alternative strategies are being developed. Some of these are based on Bacillus spp. or other insect pathogens, while others are based on the use of plant- and animal-derived genes. However, if such approaches are to play a useful role in crop protection, it is desirable that they do not have a negative impact on beneficial organisms at higher trophic levels thus affecting the functioning of the agro-ecosystem. This widely held concern over the ecological impacts of GM crops has led to the extensive examination of the potential effects of a range of transgene proteins on non-target and beneficial insects. The findings to date with respect to both commercial and experimental GM crops expressing anti-insect genes are discussed here, with particular emphasis on insect predators and parasitoids. 相似文献
75.
Kejík Z Bříza T Králová J Poučková P Král A Martásek P Král V 《Bioorganic & medicinal chemistry letters》2011,21(18):5514-5520
We present here a general system for the coordination attachment of therapeutic proteins to a drug delivery system and its application in combined therapy. Proof of concept is demonstrated by the synthesis and testing of the targeted drug delivery system for cytostatics, which is based on a combination of the drug carrier Zn-porphyrin-cyclodextrin conjugates and their supramolecular coordination complexes with immunoglobulins. This system can be as readily used for a variety of therapeutic and targeting proteins including PAs, MAs, lectins, and HSA. Moreover, it allows combined photodynamic therapy, cell targeted chemotherapy and immunotherapy. When tested in a mouse model with human C32 carcinoma, the therapeutic superiority of the coordination assembly nanosystem was shown in comparison with the efficacy of building blocks used for the construction of the system. 相似文献
76.
The mechanism that facilitates the evolution of maternal care is ambiguous in egg‐laying terrestrial vertebrates: does the ability of mothers to recognize their own eggs lead them under some circumstances to begin providing care or can maternal care evolve from simply being in close proximity to the eggs (e.g. through territorial behaviour)? This question is difficult to answer because in most species, parental care is either absent altogether or present; in only a few species we have the opportunity to observe intraspecific variation in the expression of parental care. We studied a population of long‐tailed skinks (Eutropis longicaudata) in which females have recently evolved maternal care from a noncaring state. Females on Orchid Island, Taiwan, remain with their eggs during incubation and when doing so, actively deter egg predation by egg‐eating snakes (Oligodon formosanus); in all other populations, females lack post‐ovipositional maternal care. Nest‐guarding females on Orchid Island (i) showed antipredator behaviours only in the original nest site in which they laid eggs, even after we removed all of the eggs or substituted them with those of a conspecific; (ii) protect any eggs present inside the original nest site (even when the eggs belong to a conspecific); and (iii) develop this behaviour while gravid (i.e. prior to laying eggs). This supports the hypothesis that long‐tailed skinks cannot recognize their own eggs, suggesting that maternal care is a directed form of territoriality only expressed towards egg‐eating snakes and only during reproduction. Nest guarding is among the most primitive forms of parental care, and the recent evolution of this behaviour in a single population provides insight into one of the mechanisms by which parental care can originate in terrestrial vertebrates. 相似文献
77.
The strength of trophic cascades in terrestrial habitats has been the subject of considerable interest and debate. We conducted an 8-year experiment to determine how exclusion of vertebrate predators, ungulates alone (to control for ungulate exclusion from predator exclusion plots) or none of these animals influenced how strongly a three-species assemblage of rodent consumers affected plant productivity. We also examined whether predator exclusion influenced the magnitude of post-dispersal seed predation by mice. Both ungulates and rodents had strong direct effects on graminoid biomass. However, rodent impacts on plant biomass did not differ across plots with or without predators and/or ungulates. Deer mice removed more seeds from seed depots on predator exclusion plots, suggesting trait-mediated indirect effects of predators, but this short-term behavioural response did not translate into longer-term impacts on seed survival. These results suggest that vertebrate predators do not fundamentally influence primary production or seed survival in our system. 相似文献
78.
Rui Lourenço Sara Maria Santos João Eduardo Rabaça Vincenzo Penteriani 《Population Ecology》2011,53(1):175-185
Predatory interactions among top predators, like superpredation or intraguild predation (IGP), can influence community structure.
Diurnal raptors occupy high trophic levels in terrestrial food webs, and thus can regulate the presence of mesopredators.
We studied superpredation (the killing and eating of another predator) in four large European raptors. We gathered 121 dietary
studies, totalling 161,456 prey for the Goshawk Accipiter gentilis L., Golden Eagle Aquila chrysaetos L., Bonelli’s Eagle Aquila fasciata Vieillot, and Eagle Owl Bubo bubo L. Results showed that superpredation: (1) is a widespread interaction in large raptors, but it can vary according to the
top predator species; (2) is not an important energetic resource for large raptors, but rather seems mostly related to diet
diversification when the main prey decreases; (3) is spatially clustered reflecting habitat heterogeneity, but shows no temporal
or large-scale spatial trends; and (4) it is associated with lower breeding success of the top predator species. These findings
support the food stress hypothesis as the main driving force behind increases in superpredation and IGP in raptors, with the
decrease in breeding performance as a side effect. Superpredation by large raptors deserves future research to understand
its effects on mesopredators, because on one hand it might contribute to promote biodiversity, while on the other hand, it
can sometimes represent an additional risk for small populations of endangered mesopredators. 相似文献
79.
目的:硬膜外腔注射地塞米松对腰硬联合麻醉腰背痛及神经并发症的预防效果。方法:将200例ASA I-II腰联合麻醉下择期剖宫产患者随机分成2组,A组吗啡2 mg+生理盐水稀释至10ml硬膜外腔注射;B组向硬膜外腔注射吗啡2mg+地塞米松5mg+生理盐水稀释至10 ml硬膜外腔注射。术毕两组均行静脉术后镇痛。观察并记录患者术后24,72 h的腰背痛及神经并发症情况,同时记录每例患者试穿次数。结果:B组患者术后24,72 h的背痛发生率为11.0%,4.0%,明显低于A组(P〈0.05);穿刺1次和2次及以上背痛的发生率分别为5.1%和19.5%,明显低于A组(P〈0.05)。两组72h内神经并发症差异无统计学意义。结论:硬膜外腔注射地塞米松有效预防腰硬联合麻醉剖宫产术后腰背痛,但对神经并发症的影响还有待进一步观察。 相似文献
80.
DAVID B. MACLEAN THEODORE D. SARGENT BONNIE K. MACLEAN 《Biological journal of the Linnean Society. Linnean Society of London》1989,36(3):295-311
Discriminant analysis was used to analyse the results of 348 bird-feeding trials conducted from 1982 to 1985 in Leverett, Massachusetts. Four size classes, seven appearance categories, and five larval host types, based on 163 species of moths and butterflies used as prey in two or more trials, were selected as predictor variables to discriminate between prey taken and not taken by birds. Discriminant analysis of individual feeding trials correctly classified 97.5 percent of prey taken or not-taken and ranked the predictor variables according to their relative importance in determining prey acceptability. Characteristics most acceptable to birds were: (1) large size, (2) bark-like appearance, (3) warning colouration, (4) woody generalist, (5) dead-leaf-like appearance, (6) woody specialist, and (7) medium size. Characteristics least acceptable to birds were: (1) small size, (2) mimetic appearance, (3) butterfly appearance, (4) herbaceous specialist food type, (5) black-and-white appearance, (6) extra large size, and (7) overall generalist feeder. A summary of the analyses includes a discriminant function based on lepidopteran characteristics that can be used to predict the prey acceptability of species not used in this study. A multiple regression analysis of prey taken revealed that size alone and larval host type combined with other prey characteristics were the most important variables in determining the selection of prey regardless of their abundance in the trials. 相似文献