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171.
Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays 总被引:1,自引:0,他引:1
Oono Y Seki M Satou M Iida K Akiyama K Sakurai T Fujita M Yamaguchi-Shinozaki K Shinozaki K 《Functional & integrative genomics》2006,6(3):212-234
A comparative analysis of gene expression profiles during cold acclimation and deacclimation is necessary to elucidate the molecular mechanisms of cold stress responses in higher plants. We analyzed gene expression profiles in the process of cold acclimation and deacclimation (recovery from cold stress) using two microarray systems, the 7K RAFL cDNA microarray and the Agilent 22K oligonucleotide array. By both microarray analyses, we identified 292 genes up-regulated and 320 genes down-regulated during deacclimation, and 445 cold up-regulated genes and 341 cold down-regulated genes during cold acclimation. Many genes up-regulated during deacclimation were found to be down-regulated during cold acclimation, and vice versa. The genes up-regulated during deacclimation were classified into (1) regulatory proteins involved in further regulation of signal transduction and gene expression and (2) functional proteins involved in the recovery process from cold-stress-induced damages and plant growth. We also applied expression profiling studies to identify the key genes involved in the biosynthesis of carbohydrates and amino acids that are known to play important roles in cold acclimation. We compared genes that are regulated during deacclimation with those regulated during rehydration after dehydration to discuss the similarity and difference of each recovery process.Electronic Supplementary Material Supplementary materials are available for this article at 相似文献
172.
Anthonie W. J. Muller Dirk Schulze-Makuch 《Origins of life and evolution of the biosphere》2006,36(2):177-189
Life has evolved on Earth with electromagnetic radiation (light), fermentable organic molecules, and oxidizable chemicals as sources of energy. Biological use of thermal energy has not been observed although heat, and the thermal gradients required to convert it into free energy, are ubiquitous and were even more abundant at the time of the origin of life on Earth. Nevertheless, Earth-organisms sense thermal energy, and in suitable environments may have gained the capability to use it as energy source. It has been proposed that the first organisms obtained their energy by a first protein named pF1 that worked on a thermal variation of the binding change mechanism of today's ATP sythase enzyme. Organisms using thermosynthesis may still live where light or chemical energy sources are not available. Possible suitable examples are subsurface environments on Earth and in the outer Solar System, in particular the subsurface oceans of the icy satellites of Jupiter and Saturn. 相似文献
173.
174.
A cold inducible multidomain cystatin from winter wheat inhibits growth of the snow mold fungus, Microdochium nivale 总被引:2,自引:0,他引:2
A novel cold-induced cystatin cDNA clone (TaMDC1) was isolated from cold acclimated winter wheat crown tissue by using a macroarray-based differential screening method. The deduced amino acid sequence consisted of a putative N-terminal secretory signal peptide of 37 amino acids and a mature protein (mTaMDC1) with a molecular mass of 23 kDa. The mTaMDC1 had a highly conserved N-terminal cystatin domain and a long C-terminal extension containing a second region, which exhibited partial similarity to the cystatin domain. The recombinant mTaMDC1 was purified from Escherichia coli and its cysteine proteinase inhibitory activity against papain was analyzed. The calculated Ki value of 5.8×10−7 M is comparable to those reported for other phytocystatins. Northern and western blot analyses showed elevated expression of TaMDC1 mRNA and protein during cold acclimation of wheat. In addition to cold, accumulation of the TaMDC1 message was induced by other abiotic stresses including drought, salt and ABA treatment. Investigation of in vitro antifungal activity of mTaMDC1 showed strong inhibition on the mycelium growth of the snow mold fungus Microdochium nivale. Hyphae growth was totally inhibited in the presence of 50 μg/ml mTaMDC1 and morphological changes such as swelling, fragmentation and sporulation of the fungus were observed. The mechanisms of the in vitro antifungal effects and the possible involvement of TaMDC1 in cold induced snow mold resistance of winter wheat are discussed. 相似文献
175.
The expression of a gene, encoding a dehydrin protein designated as DHN24 was analyzed at the protein level in two groups of Solanum species differing in cold acclimation ability. The DHN24 protein displays consensus amino acid sequences of dehydrins, termed K- and S-segments. The S-segment precedes three K-segments, classifying the protein into SK3-type dehydrins. A group of Solanum species able to cold acclimation constituted by S. sogarandinum and S. tuberosum, cv. Aster, and a second one composed of a S. sogarandinum line, that lost ability to cold acclimation, and of S. tuberosum, cv. Irga, displaying low ability to cold acclimation were studied. Under control conditions, noticeable levels of the DHN24 protein was observed in stems, tubers, and roots of Solanum species. No protein was detected in leaves. During low temperature treatment the DHN24 protein level substantially increased in tubers, in transporting organs and in apical parts, and only a small increase was observed in leaves. The increase in protein abundance was only observed in the plants able to cold acclimate and was found to parallel the acclimation capacity. Upon drought stress, the DHN24 level decreased in stems and in leaves, but increased in apical parts. These results suggest that Dhn24 expression is regulated by organ specific factors in the absence of stress and by factors related to cold acclimation processes during low temperature treatment in collaboration with organ-specific factors. A putative function of the SK3-type dehydrin proteins during plant growth and in the tolerance to low temperature is discussed. 相似文献
176.
177.
Summary. Complete depolymerization of actin filaments (AFs) at low temperature (0 °C) is followed by the formation of transient actin
structures at 25 °C in tobacco BY-2 cells (Nicotiana tabacum L.). Using antibodies against fission yeast actin-related proteins (ARP2 and ARP3), we show here that transient actin structures
(dots, dotted filaments, rods) colocalize with epitopes stained by these antibodies and thus are likely to represent sites
of actin filament nucleation (SANs). In contrast to the cold-induced disassembly of AFs, no transient actin structures were
detectable during recovery of AFs from latrunculin B-induced depolymerization. However, the staining pattern obtained with
ARP antibodies in latrunculin B-treated cells was similar to that in controls and cold-treated cells. This suggests that,
in addition to the complete depolymerization of AFs, disruption of other cellular structures is needed for the formation of
transient actin structures during the early phase of recovery from cold treatment.
Correspondence and reprints: Department of Plant Physiology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague
2, Czech Republic. 相似文献
178.
Mäkinen TM Raatikka VP Rytkönen M Jokelainen J Rintamäki H Ruuhela R Näyhä S Hassi J 《International journal of biometeorology》2006,51(1):27-36
The extent of outdoor exposure during winter and factors affecting it were examined in a cross-sectional population study in Finland. Men and women aged 25–74 years from the National FINRISK 2002 sub-study (n=6,591) were queried about their average weekly occupational, leisure-time and total cold exposure during the past winter. The effects of gender, age, area of residence, occupation, ambient temperature, self-rated health, physical activity and education on cold exposure were analysed. The self-reported median total cold exposure time was 7 h/week (8 h men, 6 h women),<1 h/week (2 h men, 0 h women) at work, 4 h/week (5 h men, 4 h women) during leisure time and 1 h/week (1 h men, 1.5 h women) while commuting to work. Factors associated with increased occupational cold exposure among men were: being employed in agriculture, forestry and industry/mining/construction or related occupations, being less educated and being aged 55–64 years. Factors associated with increased leisure-time cold exposure among men were: employment in industry/mining/construction or related occupations, being a pensioner or unemployed, reporting at least average health, being physically active and having college or vocational education. Among women, being a housewife, pensioner or unemployed and engaged in physical activity increased leisure-time cold exposure, and young women were more exposed than older ones. Self-rated health was positively associated with leisure time cold exposure in men and only to a minor extent in women. In conclusion, the subjects reported spending 4% of their total time under cold exposure, most of it (71%) during leisure time. Both occupational and leisure-time cold exposure is greater among men than women. 相似文献
179.
Shitzer A 《International journal of biometeorology》2006,50(4):224-232
The wind-chill index (WCI), developed in Antarctica in the 1940s and recently updated by the weather services in the USA and
Canada, expresses the enhancement of heat loss in cold climates from exposed body parts, e.g., face, due to wind. The index
provides a simple and practical means for assessing the thermal effects of wind on humans outdoors. It is also used for indicating
weather conditions that may pose adverse risks of freezing at subfreezing environmental temperatures. Values of the WCI depend
on a number of parameters, i.e, temperatures, physical properties of the air, wind speed, etc., and on insolation and evaporation.
This paper focuses on the effects of various empirical correlations used in the literature for calculating the convective
heat transfer coefficients between humans and their environment. Insolation and evaporation are not included in the presentation.
Large differences in calculated values among these correlations are demonstrated and quantified. Steady-state wind-chill-equivalent
temperatures (WCETs) are estimated by a simple, one-dimensional heat-conducting hollow-cylindrical model using these empirical
correlations. Partial comparison of these values with the published “new” WCETs is presented. The variability of the estimated
WCETs, due to different correlations employed to calculate them, is clearly demonstrated. The results of this study clearly
suggest the need for establishing a “gold standard” for estimating convective heat exchange between exposed body elements
and the cold and windy environment. This should be done prior to the introduction and adoption of further modifications to
WCETs and indices. Correlations to estimate the convective heat transfer coefficients between exposed body parts of humans
in windy and cold environments influence the WCETs and need to be standardized. 相似文献
180.
The rapid cold-hardening (RCH) response not only confers dramatic protection against cold-shock (non-freezing) injury, but also "instantaneously" enhances organismal performance. Since cold-shock injury is associated with damage to the cell membrane, we investigated the relationship between RCH and changes in cold tolerance and membrane fluidity at the cellular level. None of the adult flies (Sarcophaga bullata) in the cold-shocked treatment group survived direct transfer to -8 degrees C for 2 h; in contrast, 64.5% of flies in the RCH group survived exposure to -8 degrees C. Differences between the treatment groups also were reflected at the cellular level; only 21.3% of fat body cells in the cold-shocked group survived compared to 68.5% in the RCH group. Using 31P solid-state NMR spectroscopy, we determined that membrane fluidity increased concurrently with rapid cold-hardening of fat body cells. This result suggests that membrane characteristics may be modified very rapidly to protect cells against cold-shock injury. 相似文献