Anti-inflammatory action of herbal medicine comprised of Scutellaria baicalensis and Chrysanthemum morifolium

ABSTRACT

Various mixtures were prepared depending on the mixing ratio of Scutellaria baicalensis hot water extract (SB-HW), and Chrysanthemum morifolium ethanol extract (CM-E) and their anti-inflammatory activity were compared. Among them, SB-HW (80 μg/mL)/CM-E (120 μg/mL) or SB-HW (40 μg/mL)/CM-E (160 μg/mL) significantly inhibited LPS-stimulated NO and IL-6 levels in RAW 264.7 cells. The SB-HW (80 μg/mL)/CM-E (120 μg/mL) mixture, which was determined as active mixture, significantly reduced MUC5AC secretion in PMA and LPS-induced NCI-H292 cells. The active mixture also reduced the production of PGE₂ and IL-8 in PMA-induced A549 cells. LC-MS/MS analysis showed that the active mixture was composed of high contents of flavone glycosides, such as baicalin and cynaroside. Western blot analysis indicated that the active mixture suppressed phosphorylation of ERK, JNK, and p38, associating with the inhibition of MAPK signaling. Taken together, our results suggest that the active mixture could be applied as a new anti-inflammatory herbal medicine.     

Genetic improvement of pyrethrum

Summary Pyrethrum (Chrysanthemum cinerariefolium), an important paramedicinal plant is a potential source of pyrethrins, which have a long history of safe uses against mosquito larvae — a carrier of malarial parasite. It was introduced in India from Kenya in 1931. Considerable genetic diversity has been generated over the years. Repeated clonal selection could lead to isolation of a number of divergent clones representing selective divergence. Planned hybridization among some of the chosen clones could further enlarge the spectrum of variation as measured by multivariate analyses (D²-statistic and canonical analysis). The resulting hybrids manifested a variable degree of heterosis which was found to be, by and large, positively associated with the degree of divergence between the two constituent parents of a hybrid. However, the choice of the potential hybrid clone(s) for commercial exploitation was most viable when parents for hybridization were short-listed on the basis of parental divergence coupled essentially with per se performance for specific traits. The latter criterion assumes greater significance since low x low or medium x low parental hybrids also tended to register high heterosis for both the pyrethrins content and yield. Four hybrids: 234xL, 8xL, 326×395 and 319xL were identified to be the most promising for clonal selection.CIMAP publication no. 9189