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31.
Mitsuo Satoh Shinji Hosoi Seiji Sato 《In vitro cellular & developmental biology. Plant》1990,26(11):1101-1104
Summary The protease activity in serum-free conditioned medium of chinese hamster ovary (CHO) cells was measured using peptidyl (or
aminoacyl)-4-methylcoumaryl-7-amides (MCAs) as the substrates. Aminopeptidase increased in level as amounts of nonviable cells
increased during cultivation in serum-free medium, indicating that the activity seems to be originated from intracellular
proteases. The activity toward Boc-Leu-Arg-Arg-MCA, which was strongly inhibited by p-chloromercuribenzonate and N-ethylmaleimide,
was the strongest among those toward peptidyl-MCAs in the conditioned medium within 48 h-cultivation in serum-free medium.
In contrast to the case of aminopeptidase activity, the endopeptidase activity decreased in level after 48 h-cultivation although
amounts of nonviable cells increases. Thus, CHO cells continuously secrete the cysteine proteases.
This work was supported by the management of the Research Association for Biotechnology as a part of the R&D of Basic Technology
for Future Industries sponsored by NEDO (New Energy and Industrial Technology Development Organization). 相似文献
32.
腐霉属分类性状评价及其中国的种 总被引:1,自引:0,他引:1
本文对腐霉属Pythium Pringsheim的研究历史作了简单的回顾,对该属的分类性状和系统进行了论述和评价,最后对中国已发现的55种腐霉,以检索表的方式进行了分类、检索。 相似文献
33.
Chae Oh Lim Soo In Lee Woo Sik Chung Sung Han Park Inhwan Hwang Moo Je Cho 《Plant molecular biology》1996,30(2):373-379
A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root. 相似文献
34.
用胶原酶法分离胰岛细胞、超速离心法提取中国地鼠胰岛和肝脏细胞膜蛋白,SDS电泳、WesternBlotting分离GLUT后,应用GLUT2抗体免疫结合法测定GLUT的含量与分子量,同时测定了动物血糖、尿糖和血浆胰岛素的含量、显微镜下直接计数了胰岛细胞总数。结果表明:NIDDM发病后,自发NIDDM中国地鼠胰岛细胞GLUT含量减少,与GLUT2抗体反应明显减弱,但肝脏GLUT无明显变化。胰岛细胞总数发病前后大体一致。而血糖、尿精及血浆胰岛素浓度明显升高。 相似文献
35.
本文报道小剂量碳酸锂对自发的遗传性糖尿病模型中国地鼠(NIDDM)三个月处理疗效观察。采用胰岛素敏感性指数——空腹血浆胰岛素与血糖乘积的倒数,判断药物疗效。结果显示:碳酸锂使糖尿病鼠血糖降低,血胰岛素无变化,胰岛素敏感性增强。 相似文献
36.
Abstract: cis -Methyldioxolane (CD) is a muscarinic receptor agonist. [3 H] CD has been used to label a subpopulation of muscarinic receptors described as exhibiting high agonist affinity. Pharmacological evidence suggests that the population of receptors labeled by [3 H] CD consists of m2 and/or m4 subtypes; however, no studies have directly addressed the subtype selectivity of [3 H] CD. The present study characterizes binding of this ligand to individual human receptor subtypes expressed in transfected Chinese hamster ovary cells. Results indicate that [3 H] CD binds with high affinity only to Hm2 receptors but not to all Hm2 receptors. Twenty-eight percent of Hm2 receptors bound [3 H] CD with a K D of 3.5 ± 0.5 nM. Binding was eliminated in the presence of guanosine 5'- O -(3-thiotriphosphate), indicating that the Hm2 receptors labeled by [3 H] CD are those that are associated with GDP-bound G protein. Binding of [3 H] CD by only a subpopulation of Hm2 receptors is in agreement with data generated from studies of [3 H] CD binding in mammalian brain. Because muscarinic receptors have been implicated to play a role in the pathogenesis of both Alzheimer's and Parkinson's disease, as well as the neurotoxicity of organophosphorus compounds, knowledge of the binding specificity of the muscarinic agonist [3 H] CD should aid research in these areas. 相似文献
37.
田林老山中山两类森林凋落物研究 总被引:15,自引:2,他引:13
田林老山中山两类森林凋落物研究梁宏温(广西农学院林学分院,南宁530001)StudiesontheLitterfallofTwoForestTypcsinMid—AltitudeofLaoshanMountaininTianlinCounty.¥L... 相似文献
38.
Alwin Krmer Jan Pudil Heinz Frank Franz Oesch Handruedi Glatt 《Mutation research》1993,290(2):165-174
Trans-stilbene oxide, trans-β-methylstyrene, 7,8-oxide, trans-β-ethylstyrene, 7,8-oxide, trans-β-propylstyrene 7,8-oxide and 4-fluorochalcone oxide were investigated for genotoxic activity in bacterial and mammalian cells, in the absence of external xenobiotic-metabolising systems. All compounds strongly enhanced the frequency of sister-chromatid exchanges (SCE) in cultured human lymphocytes. None of them was mutagenic in Salmonella typhimurium (reversion of the his− strains TA98, TA100 and TA104). The limit of detection was 1/20,000 to 1/106 of the activity of the positive control, benzo[a]pyrene 4,5-oxide, depending on the compound and the bacterial strain. Trans-β-methylstyrene 7,8-oxide and 4-fluorochalcone oxide were additionally tested for induction of SCE and gene mutations in the same target cells, namely Chinese hamster V79 cells. Their influence on the level of SCE was similar to that observed in human lymphocytes, whilst gene mutations (at the hprt locus) were not induced. The four investigated styrene oxide derivatives are known to be excellent substrates for a mammalian enzyme, cytosolic epoxide hydrolase (cEH). 4-Fluorochalcone oxide is a potent selective inhibitor of this enzyme and is structurally similar to the investigated styrene oxide derivatives. These properties of the test compounds however cannot explain the observed discrepancies in the results, since the genetic end point (SCE versus gene mutations) was decisive, and SCE were induced in cEH-proficient human lymphocytes as well as in cEH-deficient V79 cells. 相似文献
39.
Heavy metals in some Chinese herbal plants 总被引:1,自引:0,他引:1
The concentrations of nine heavy metals, cadmium, cobalt, copper, iron, manganese, nickel, lead, zinc and mercury in 42 Chinese
herbal medicinal plants were determined. Generally, all the samples studied had, relative to the other trace metals, higher
concentrations of iron, manganese, and zinc. The concentration range of the metals determined was comparable to that in many
of the East Asian vegetables and fruits. A few samples were found to contain relatively higher concentrations of the toxic
metals such as cadmium, lead, and mercury. This was probably caused by contamination during air-drying and preservation. 相似文献
40.
中国瑟姬小蜂及二新种的描述(膜翅目:姬小蜂科) 总被引:1,自引:0,他引:1
瑟姬小蜂属Cirrospilus昆虫是鳞翅目,双翅目,特别是细蛾,潜叶蛾,潜叶蝇类及其他荫蔽性小幼虫或蛹的重要寄生蜂,有些也能重寄生于绒茧蜂和蜘蛛卵等中,本文除二新种描述外,还收入瑟姬小蜂六种。一并列于检索表中。 相似文献