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41.
Annie Giraud Simone Bouchilloux 《Biochemical and biophysical research communications》1983,111(2):353-359
The synthesis of glycosaminoglycans (GAGs) was investigated in porcine thyroid cells under the influence or not of thyrotropin. After labelling with [3H] glucosamine and [35S] , enriched GAG-fractions prepared from culture media, cells, and eventually substrate adhering materials, were analyzed by cellulose acetate electrophoresis combined with specific degradations. They comprised heparan sulfate and hyaluronic acid together with an unknown sulfated component labile to endo-β-galactosidase. Whereas global labellings of newly made GAGs were not significantly modified by thyrotropin, we reproducibly observed with the hormone a substantial increase in the proportion of hyaluronic acid [3H] label and, when cells organized into follicles, of the proportion of cell-associated [3H] GAGs. This system thus offers an interesting model to study how the responsiveness to an hormone and the reorganization that follows might implicate specific glycoconjugates. 相似文献
42.
Larry E. Dillehay Larry H. Thompson Jason L. Minkler Anthony V. Carrano 《Mutation research》1983,109(2):283-296
The majority of the high (12-fold elevated) baseline sister-chromatid exchanges (SCEs) that occur in the CHO mutant line EM9 appear to be a consequence of incorporated BrdUrd, and they arise during replication of DNA containing BrdUrd in a template strand. In normal CHO cells the alkaline elution patterns of DNA newly replicated on a BrdUrd-containing template are significantly altered compared with those seen during the replication on an unsubstituted template. The nascent DNA synthesized on such an altered template is delayed in reaching mature size, possibly because replication forks are temporarily blocked at sites occurring randomly along the template. Transient blockage of replication forks may be a prerequisite for SCE. The delay in replication on BrdUrd-substituted templates was greater in EM9 cells than in parental AA8 cells and was also greater in AA8 cells treated with benzamide, an inhibitor of poly(ADPR) polymerase, than in untreated AA8 cells. Under these conditions, treatment with benzamide also produced a 7-fold increase in SCEs in AA8. An EM9-derived revertant line that has a low baseline SCE frequency showed less delay in replication on BrdUrd-substituted templates than did EM9. However, under conditions where the template strand contained CldUrd, which was shown to produce 4-fold more SCEs than BrdUrd in AA8 cells, the replication delay in AA8 was not any greater in the CldUrd-substituted cells. Thus, other factors besides the delay appear to be involved in the production of SCEs by the template lesions resulting from incorporation of the halogen-substituted pyrimidine molecules. 相似文献
43.
Judith M. Clarkson David L. Mitchell 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1983,740(4):355-361
The effect on DNA repair of several inhibitors of DNA synthesis has been investigated in CHO cells. Three assays were employed following ultraviolet irradiation of G1 cells: unscheduled DNA synthesis, removal of antibody binding sites and alkaline elution. Cytosine arabinoside and aphidicolin were found to reduce unscheduled DNA synthesis in a dose-dependent manner without affecting the removal of antibody-binding sites. Strand rejoining was also inhibited. These results are consistent with the hypothesis that inhibition is due to premature chain termination during repair synthesis some time after excision of the lesion. Conversely, inhibition of unscheduled DNA synthesis by novobiocin is paralleled by inhibition of excision of the lesion. However, no inhibition of incision was apparent. Since nalidixic acid, an inhibitor of topoisomerase II, did not inhibit excision, it is unlikely that the primary site of action of novobiocin is this topoisomerase. The possibility that a second topoisomerase and/or a polymerase are affected is discussed in the light of previously published data. 相似文献
44.
Dr. W. B. Watkins 《Cell and tissue research》1974,155(2):201-210
Summary The immunoglobulin-enzyme bridge technique, in association with rabbit antiporcine neurophysin-II has been applied to the median eminence of the dog and cat in order to study the distribution of neurophysin-like antigens throughout this area of the brain and correlate the findings with the corresponding distribution of neurosecretory material (NSM) as revealed by the crotonaldehyde fuchsin stain.Neurophysin and NSM were both present in the hypothalamo-supraoptico-neurohypophysial system—the pathway taken by oxytocin, vasopressin and neurophysin from the hypothalamus to the posterior pituitary lobe.Whereas the tuberoinfundibular tract of the median eminence was almost devoid of NSM, the presence of neurophysin-like material was clearly evident using immunoperoxidase histochemistry. The significance of a protein in the external median eminence possessing determinants cross-reactive against anti-neurophysin serum is discussed.This work was financed by a grant from the Medical Research Council of New Zealand. 相似文献
45.
Hans-Arne Hansson 《Cell and tissue research》1970,105(2):242-251
Summary The epithelial cells in the retina of rats were examined by scanning and transmission electron microscopy.The apical surface of the epithelial cells is covered by a large number of microvilli, which are embedded in an extracellular material. Distinct holes remaining after detached photoreceptor cell segments are numerous. The cells are polyhedronal, usually hexagonal and holes are scattered in their plasma membrane. Many of the epithelial cells are binucleated. The borders between adjacent cells are always close to each other. Numerous cytoplasmic folds or foot processes occur at the base of the cells as well as an extracellular space.The characteristic surface structures are discussed in relation to the function of the retinal epithelial cells.Supported by grants from the Swedish Medical Research Council (B70-12X-2543-02) and Riksföreningen mot Cancer (69171, 265-B69-01X). 相似文献
46.
47.
猕猴桃种间杂交结果初报 总被引:4,自引:0,他引:4
王圣梅;武显维;黄仁煌;熊治廷;柯善强 《武汉植物学研究》1989,7(4):399-402
目前,国内外栽培的猕猴桃品种均为系统选育而成。采用种间杂交育种,可获得比系统选育更为理想的新型栽培品种。开展猕猴桃远缘杂交研究己见报道的国外有新西兰,国内有郑州果树研究所、北京植物园等,但至今未见有开花结果的资料报道。为了 相似文献
48.
Versieck Jacques Vanballenberghe Lidia Wittoek Ann Vermeir Gerda Vandecasteele Carlo 《Biological trace element research》1990,(1):683-689
A method is described for the determination of mercury in human blood serum and packed blood cells employing neutron activation
analysis. Great attention was devoted to the collection and manipulation of the samples. The accuracy and precision of the
method were tested by analyzing biological reference materials and by comparing the concentrations measured in a number of
serum samples to those obtained by another, independent technique (cold vapor atomic absorption spectrometry) in the same
samples. The article reports the levels measured in blood serum and packed blood cells samples from 15 adult volunteers, as
well as the figures determined in a “second-generation” biological reference material (freeze-dried human serum), prepared
and conditioned at the University of Ghent. 相似文献
49.
黄连木五倍子的生态研究与利用 总被引:1,自引:0,他引:1
一、产地自然概况黄连木五倍子最早发现于河南省南部信阳市郊贤山脚下(东经114°0′,,北纬32°10′)。该地为低山丘陵,海拔90一320m,南部紧连鸡公山厂傍依南湾水库和狮河。 相似文献
50.
中华猕猴桃胚乳植株后代的观察 总被引:2,自引:0,他引:2
对438株定植的中华猕猴桃胚乳培.养的试管苗,经四年的田间观察,并进行连续二年结果分析。与对照的母株相比,胚乳植株在株形、叶片大小、果实形态及果实的主要营养成分含量上都有较大的变化。同时还发现,由同一块愈伤组织诱导的胚乳试管苗后代中也有雌、雄性别的分化。胚乳植株后代的多样性,可为中华猕猴桃的选种及品种繁育提供丰富的材料。 相似文献