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21.
Manh Huynh Vu Tetsu Ando Shigeo Yoshida Nobutaka Takahashi Sadahiro Tatsuki Kazumasa Katagiri 《Bioscience, biotechnology, and biochemistry》2013,77(7):1615-1617
Ferredoxin-nitrite reductase (EC 1.7.7.1), an enzyme which catalyzes the 6-electron reduction of nitrite to ammonia, has been isolated from Spinacia oleracea. The isolated enzyme was homogeneous by disc electrophoresis with polyacrylamide gel. The molecular weight of the enzyme was estimated to be 86,000 by Ultrogel AcA 34 gel filtration. In the oxidized form, the enzyme had absorption maxima at 278, 388 (Soret band), 573 (α band) and 690 nm, indicating that siroheme is directly involved in the catalysis of nitrite reduction. This absorption spectrum was modified by sulfite, hydroxylamine and cyanide. The enzyme exhibited electron paramagnetic resonance signals with g values of 6.9 and 5.2, which are characteristic of a high spin Fe3+ -siroheme in the molecule. These signals disappeared upon the addition of dithionite or nitrite. This isolated enzyme also contained four moles of labile sulfide and 7 g-atoms of iron per 86,000 g of protein. 相似文献
22.
In the course of study on citric acid fermentation by Candida tropicalis KY6224, in which n-alkane mixture (C–12 to C–15) was used as the sole source of carbon, we found that a arabitollike substance was accumulated when the medium-pH was controlled at low level (3.0 to 4.0). This substance was isolated in crystalline forms and identified as d-arabitol.d-Arabitol production was also observed with ethanol, acetic acid and glucose as the sole source of carbon. Important factors for efficient production of d-arabitol were keeping the medium-pH at low-level (3.0 to 4.0) and the concentration of NaCl or KCl at high level (1 to 5%). This strain produced 75 mg/ml of d-arabitol in 120 hr incubation under optimal culture conditions; this corresponds to 50 % of n-alkane consumed. 相似文献
23.
长白山区阔叶红松林经营历史与研究历程 总被引:3,自引:0,他引:3
长白山区位于中国东北东部的山地森林区,地带性植被是阔叶红松林,是我国珍贵木材生产和东北森林带的重要载体,孕育着丰富的物种资源.短短120年来,长白山区广袤的原始森林几乎消失殆尽,被次生林、过伐林和人工林等替代.建国以来,东北的阔叶红松林经营理论与技术在红松更新规律、皆伐与择伐的争论中,在人们对阔叶红松林结构和功能认识不断深入的过程中逐渐完善.但目前红松更新问题依然存在,森林经营依然十分粗放.本文梳理了过去70年来阔叶红松林的经营历史及研究历程,分析当前森林资源类型和经营制度的形成过程,以期为该区天然林可持续经营和质量的精准提升等提供借鉴. 相似文献
24.
2006—2017年长白山阔叶红松林木本植物种子雨的时空动态 总被引:1,自引:0,他引:1
种子是森林更新的基础.为研究长白山阔叶红松林种子雨组成及时空变化,在长白山阔叶红松林25 hm2样地内设置150个面积为0.5 m2的收集器.自2006年5月至2017年12月收集252次,共收集到成熟、未成熟种子764299粒,隶属12科17属27种,其中,主林层树种13种704231粒,占总数的90%以上.种子最多的4个树种依次为紫椴、水曲柳、色木槭、假色槭,每年各个收集器都收集到这4个树种的种子;种子雨大爆发现象在各林层树种均存在,次林层和林下层较主林层树种滞后1~2年;各林层树种集中在秋季产生种子,各树种的种子雨都存在较大的时空变异性,相对而言,多数树种的空间变异性大于时间变异性;与科罗拉多岛(BCI)热带森林样地和浙江古田山亚热带常绿林样地种子产量的年际变异系数相比,长白山阔叶红松林种子产量年际变异系数较大,支持高纬度地区种子产量变异性高于低纬度地区的假说. 相似文献
25.
《Saudi Journal of Biological Sciences》2022,29(4):2238-2246
Spinach (Spinacia oleracea L.) is considered a nitrogen (N) intensive plant with high nitrate (NO3?) accumulation in its leaves. The current study via a two-year field trial introduced an approach by combining N fertilization from different sources (e.g., ammonium nitrate; 33.5 % N, and urea; 48 % N) at different rates (180, and 360 kg N ha?1) with the foliar spraying of molybdenum (Mo) as sodium molybdate, and/or manganese (Mn) as manganese sulphate at rates of 50 and 100 mgL?1 of each or with a mixture of Mo and Mn at rates of 50 and 50 mg L?1, respectively on growth, chemical constituents, and NO3? accumulation in spinach leaves. Our findings revealed that the highest rate of N fertilization (360 kg N ha?1) significantly increased most of the measured parameters e.g., plant length, fresh and dry weight plant?1, number of leaves plant?1, leaf area plant?1, leaf pigments (chlorophyll a, b and carotenoids), nutrients (N, P, K, Fe, Mn, Zn), total soluble carbohydrates, protein content, net assimilation rate, and NO3? accumulation, but decreased leaf area ratio and relative growth rate. Moreover, plants received urea-N fertilizer gave the highest values of all previous attributes when compared with ammonium nitrate –N fertilizers, and the lowest values of NO3? accumulation. The co-fertilization of N-Mo-Mn gave the highest values in all studied attributes and the lowest NO3? accumulation. The best treatment was recorded under the treatment of 360 kg N-urea ha?1 in parallel with the combined foliar application of Mo and Mn (50 + 50 mg L?1). Our findings proposed that the co-fertilization of N-Mo-Mn could enhance spinach yield and its quality, while reducing NO3? accumulation in leaves, resulting agronomical, environmental and economic benefits. 相似文献
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28.
Yoshinori Asakawa Masao Toyota Tsunematsu Takemoto Claude Suire 《Phytochemistry》1979,18(8):1355-1357
Four novel secoaromadendrane-type sesquiterpene hemiacetals, plagiochilines C, D, E and F, together with the previously known (?)-bicyclogermacrene, have been isolated from the liverwort, Plagiochila asplenioides and their structures have been spectroscopically elucidated. From Plagiochila semidecurrens plagiochilines A and C have been obtained along with (?)-bicyclogermacrene and its related sesquiterpene hydrocarbons. 相似文献
29.
东北山地水生鞘翅目昆虫多样性的比较研究 总被引:4,自引:0,他引:4
调查了东北长白山自然保护区及辽宁省医巫闾山两地静水水体中水生甲虫.结果表明,长白山阔叶红松林生态系统水生甲虫比辽宁医巫闾山农林复合生态系统水生甲虫物种丰富的多.长白山水生甲虫共有17个属29个种,而辽宁医巫闾山水生甲虫只有10个属13个种.两地水生甲虫Shannon多样性指数分别为2.124、1.643,Shannon均匀度分别为1.260、0.641,两种不同生态系统中的水生甲虫物种多度分布较好地拟合于对数级数模型,均以少数物种为优势种,其中长白山自然保护区水生甲虫以Hydroglyphusjaponicus、Haliplussimplex为优势种,辽宁医巫闾山水生甲虫则以Hydroglyphuspusilus、Agabususuriensis、Agabusbrandti占优势. 相似文献
30.
长白山林区森林/沼泽交错群落的植物多样性 总被引:21,自引:0,他引:21
本研究应用样带网格调查方法,对森林/沼泽交错区上6种群落的植物多样性状况、优势种类与分布以及交错区环境梯度进行了研究。结果表明:森林/沼泽交错区植物多样性具有沿着交错区环境梯度逐渐增高的趋势;发育成熟的交错群落具有最高的植物多样性,且高于相对应的典型森林群落。由于交错区群落存在着沼泽植物类群、森林类群以及交错区群落的优势种类群,故发育成熟的交错区群落种类较丰富,一些优势种种群数量为森林中的5倍。因为交错区的生境对于满足这些种类的生活史具有重要作用。森林/沼泽交错群落的特征与交错区环境梯度以及两个植被类型的特征密切相关。 相似文献