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71.
70例颈椎病的TCD研究 总被引:2,自引:0,他引:2
王珏 《上海生物医学工程》2000,(4):36-37,7
目的:探讨颈椎病是否引起椎动脉(VA)的血液动力学异常。材料与方法:用TCD仪对70例颈椎病患者进行检查,指标:频谱、Vs、Vd、Vm、PI,与正常人组50人进行比较。结果:颈椎病组>40岁年龄组的指标与正常人相应年龄组差异显著(P<0.01~0.05),<40岁组无显著性差异(P>0.05),异常改变主要为单侧的VA,颈椎病组异常的VA通常有频谱的相应改变。结论:颈椎病患者TCD的改变一般为单侧的VA流速降低为主要特点。 相似文献
72.
【背景】番茄黄化曲叶病毒(TYLCV)是由媒介昆虫烟粉虱传播的一种双生病毒,对蔬菜及烟草等经济作物造成严重危害。前人资料表明,该病毒于2006年传人我国南方地区,2007年传人山东省,2008年后在山东各地逐渐蔓延扩散。【方法】为了考证TYLcV传人山东省的时间,本研究利用mtCOI基因对于2005和2006年7—8月份在山东省不同地区作物上共采集的15份烟粉虱样品进行了生物型鉴定,并进一步检测了烟粉虱携带TYLCV情况,同时对PCR扩增产物进行了测序分析。【结果】2005年的4份样品烟粉虱生物型均为B型,均不携带TYLCV。2006年的11份烟粉虱样品为B型与Q型混合样品,其中,2份烟粉虱样品检测到TYLCV,进一步证实该病毒为TYLCV。【结论与意义】本研究首次证实了TYLCV早在2006年就已经传入山东省。研究结果不仅对于防控该病毒具有重要指导意义,而且对于其入侵生物学研究也具有重要参考价值。 相似文献
73.
Nitrogen oxide-containing compounds displaced the peripheral benzodiazepine ligand [3H]Ro5-4864 from guinea pig membrane preparations. Sodium nitroprusside (SNP) was the most potent (IC50 = 5.61 ± 1.72 × 10−5 M). Moreover, its ability to bind to these receptors showed marked tissue variability (heart> kidney cerebral cortex). When tested on rat atrium, SNP by itself had no effect on basal inotropy or the increase in inotropy induced by (−)-S-BAY K 8644. In contrast, Ro5-4864 potentiated the marked increase in inotropy induced by (−)-S-Bay K 8644, and SNP completely abolished the potentiation of inotropy observed with Ro5-4864. Since peripheral benzodiazepine receptors are associated with calcium mobilization in the heart, these findings may indicate that some of the clinical effects of nitric oxide-generating drugs could be mediated by these receptors. 相似文献
74.
A study was performed to validate 3 FMDV 3ABC-I-ELISA kits developed in China for the differentiation of FMDV infected and vaccinated animals. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits, Ceditest® FMDV-NS (Ceditest® kit), UBI® FMDV NONSTRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI® kit) and a FMDV 3ABC-I-ELISA kit developed at the Lanzhou Veterinary Research Institute. The test parameters (sensitivity and specificity) of the three kits were determined, and the result obtained from FMD 3ABC-I-ELISA kit was compared with that obtained from two foreign kits. The results indicated that the coincidence rate between the FMDV 3ABC-I-ELISA and Ceditest® kits was 98.05%, and the coincidence rate between the FMDV 3ABC-I-ELISA and UBI® kits was 94.4%; the sensitivity of both Ceditest® and FMDV 3ABC-I-ELISA kit was 100%. However, the sensitivity of the UBI® kit was only 81.8%. With sera from naive or vaccinated non-infected animals, the specificity of all tests exceeded 90%. 相似文献
75.
近年,我国学者在水稻(Oryza sativa)广谱抗病机制、抗性与产量协调机制等方向的研究取得了一系列突破性进展。最近,何祖华和杨卫兵团队在水稻抗病性研究中再次取得突破,发现1个以钙感应蛋白ROD1和过氧化氢酶CatB为核心的信号轴,通过负调控水稻免疫反应,保证正常生长条件下维持低水平的免疫反应,促进水稻正常生长发育... 相似文献
76.
77.
对13例新石器时代人类的牙槽疾病进行了分析,发现龋病、牙槽萎缩、磨损为主要疾病。中国口腔矫形史有理由可追溯到商代末期。 相似文献
78.
79.
Peter S. Thuy-Boun Ana Y. Wang Ana Crissien-Martinez Janice H. Xu Sandip Chatterjee Gregory S. Stupp Andrew I. Su Walter J. Coyle Dennis W. Wolan 《Molecular & cellular proteomics : MCP》2022,21(3):100197
The gut microbiota plays an important yet incompletely understood role in the induction and propagation of ulcerative colitis (UC). Organism-level efforts to identify UC-associated microbes have revealed the importance of community structure, but less is known about the molecular effectors of disease. We performed 16S rRNA gene sequencing in parallel with label-free data-dependent LC-MS/MS proteomics to characterize the stool microbiomes of healthy (n = 8) and UC (n = 10) patients. Comparisons of taxonomic composition between techniques revealed major differences in community structure partially attributable to the additional detection of host, fungal, viral, and food peptides by metaproteomics. Differential expression analysis of metaproteomic data identified 176 significantly enriched protein groups between healthy and UC patients. Gene ontology analysis revealed several enriched functions with serine-type endopeptidase activity overrepresented in UC patients. Using a biotinylated fluorophosphonate probe and streptavidin-based enrichment, we show that serine endopeptidases are active in patient fecal samples and that additional putative serine hydrolases are detectable by this approach compared with unenriched profiling. Finally, as metaproteomic databases expand, they are expected to asymptotically approach completeness. Using ComPIL and de novo peptide sequencing, we estimate the size of the probable peptide space unidentified (“dark peptidome”) by our large database approach to establish a rough benchmark for database sufficiency. Despite high variability inherent in patient samples, our analysis yielded a catalog of differentially enriched proteins between healthy and UC fecal proteomes. This catalog provides a clinically relevant jumping-off point for further molecular-level studies aimed at identifying the microbial underpinnings of UC. 相似文献
80.
Brain Cytochrome Oxidase in Alzheimer''s Disease 总被引:5,自引:0,他引:5
Stephen J. Kish Catherine Bergeron Ali Rajput† Slobodan Dozic‡ Frank Mastrogiacomo Li-Jan Chang Julie M. Wilson Linda M. DiStefano Jose N. Nobrega§ 《Journal of neurochemistry》1992,59(2):776-779
A recent demonstration of markedly reduced (-50%) activity of cytochrome oxidase (CO; complex 4), the terminal enzyme of the mitochondrial enzyme transport chain, in platelets of patients with Alzheimer's disease (AD) suggested the possibility of a systemic and etiologically fundamental CO defect in AD. To determine whether a CO deficiency occurs in AD brain, we measured the activity of CO in homogenates of autopsied brain regions of 19 patients with AD and 30 controls matched with respect to age, postmortem time, sex, and, as indices of agonal status, brain pH and lactic acid concentration. Mean CO activity in AD brain was reduced in frontal (-26%: p less than 0.01), temporal (-17%; p less than 0.05), and parietal (-16%; not significant, p = 0.055) cortices. In occipital cortex and putamen, mean CO levels were normal, whereas in hippocampus, CO activity, on average, was nonsignificantly elevated (20%). The reduction of CO activity, which is tightly coupled to neuronal metabolic activity, could be explained by hypofunction of neurons, neuronal or mitochondrial loss, or possibly by a more primary, but region-specific, defect in the enzyme itself. The absence of a CO activity reduction in all of the examined brain areas does not support the notion of a generalized brain CO abnormality. Although the functional significance of a 16-26% cerebral cortical CO deficit in human brain is not known, a deficiency of this key energy-metabolizing enzyme could reduce energy stores and thereby contribute to the brain dysfunction and neurodegenerative processes in AD. 相似文献