全文获取类型
收费全文 | 2745篇 |
免费 | 99篇 |
国内免费 | 77篇 |
出版年
2023年 | 78篇 |
2022年 | 108篇 |
2021年 | 154篇 |
2020年 | 86篇 |
2019年 | 88篇 |
2018年 | 91篇 |
2017年 | 37篇 |
2016年 | 37篇 |
2015年 | 57篇 |
2014年 | 115篇 |
2013年 | 123篇 |
2012年 | 73篇 |
2011年 | 75篇 |
2010年 | 59篇 |
2009年 | 85篇 |
2008年 | 119篇 |
2007年 | 97篇 |
2006年 | 93篇 |
2005年 | 57篇 |
2004年 | 72篇 |
2003年 | 66篇 |
2002年 | 60篇 |
2001年 | 61篇 |
2000年 | 66篇 |
1999年 | 64篇 |
1998年 | 43篇 |
1997年 | 78篇 |
1996年 | 64篇 |
1995年 | 68篇 |
1994年 | 65篇 |
1993年 | 60篇 |
1992年 | 59篇 |
1991年 | 38篇 |
1990年 | 49篇 |
1989年 | 52篇 |
1988年 | 36篇 |
1987年 | 33篇 |
1986年 | 30篇 |
1985年 | 27篇 |
1984年 | 33篇 |
1983年 | 23篇 |
1982年 | 28篇 |
1981年 | 25篇 |
1980年 | 12篇 |
1979年 | 10篇 |
1977年 | 8篇 |
1976年 | 12篇 |
1972年 | 11篇 |
1971年 | 7篇 |
1970年 | 7篇 |
排序方式: 共有2921条查询结果,搜索用时 15 毫秒
151.
152.
153.
154.
155.
156.
157.
The mouse primary visual cortex (V1) has emerged as a classical system to study neural circuit mechanisms underlying visual function and plasticity. A variety of efferent-afferent neuronal connections exists within the V1 and between the V1 and higher visual cortical areas or thalamic nuclei, indicating that the V1 system is more than a mere receiver in information processing. Sensory representations in the V1 are dynamically correlated with neural activity oscillations that are distributed across different cortical layers in an input-dependent manner. Circuits consisting of excitatory pyramidal cells (PCs) and inhibitory interneurons (INs) are the basis for generating neural oscillations. In general, INs are clustered with their adjacent PCs to form specific microcircuits that gate or filter the neural information. The interaction between these two cell populations has to be coordinated within a local circuit in order to preserve neural coding schemes and maintain excitation–inhibition (E–I) balance. Phasic alternations of the E–I balance can dynamically regulate temporal rhythms of neural oscillation. Accumulating experimental evidence suggests that the two major sub-types of INs, parvalbumin-expressing (PV+) cells and somatostatin-expressing (SOM+) INs, are active in controlling slow and fast oscillations, respectively, in the mouse V1. The review summarizes recent experimental findings on elucidating cellular or circuitry mechanisms for the generation of neural oscillations with distinct rhythms in either developing or matured mouse V1, mainly focusing on visual relaying circuits and distinct local inhibitory circuits. 相似文献
158.
The present study characterized the receptor‐dependent regulation of dendrite formation of noradrenaline (NA) and dopamine (DA) in cultured neurons obtained from embryonic day 16 rat cerebral cortex. Morphological diversity of cortical dendrites was analyzed on various features: dendrite initiation, dendrite outgrowth, and dendrite branching. Using a combination of immunocytochemical markers of dendrites and GABAergic neurons, we focused on the dendrite morphology of non‐GABAergic neurons. Our results showed that (1) NA inhibited the dendrite branching, (2) β adrenergic receptor (β‐AR) agonist inhibited the dendrite initiation, while promoted the dendrite outgrowth, (3) β1‐AR and β2‐AR were present in all the cultured neurons, and both agonists inhibited the dendrite initiation, while only β1‐AR agonist induced the dendrite branching; (4) DA inhibited the dendrite outgrowth, (5) D1 receptor agonist inhibited the dendrite initiation, while promoted the dendrite branching. In conclusion, this study compared the effects of NA, DA and their receptors and showed that NA and DA regulate different features on the dendrite formation of non‐GABAergic cortical neurons, depending on the receptors. © 2012 Wiley Periodicals, Inc. Develop Neurobiol 73: 370–383, 2013 相似文献
159.
《Fly》2013,7(1):12-15
The ability of the microtubule cytoskeleton to rapidly and locally reorganize itself in response to intra- and extracellular signals is essential to its wide range of functions. A site of tightly regulated microtubule dynamics—and the major interface between the microtubule cytoskeleton and the extracellular environment—is the cell cortex, where the selective stabilization and destabilization of microtubule plus-ends is required for normal cell division, morphogenesis and migration. In a recent study, we found that the cortex of Drosophila S2 and D17 cells is coated with the microtubule severing enzyme and plus-end depolymerase, Kat-60, which actively suppresses microtubule growth and stability along the cell edge. We have proposed that cortical Kat-60 functions by uncapping plus-ends, thereby activating another microtubule depolymerase, KLP10A, preloaded onto the end. The localized destruction of microtubule plus-ends at a specific cortical could feed into larger regulatory pathways, such as those in control of the actin cytoskeleton, to influence cell polarization and motility. 相似文献
160.
Robert Bronstein Luisa Torres Jillian C. Nissen Stella E. Tsirka 《Journal of visualized experiments : JoVE》2013,(78)
Microglia are the resident macrophage-like cells of the central nervous system (CNS) and, as such, have critically important roles in physiological and pathological processes such as CNS maturation in development, multiple sclerosis, and spinal cord injury. Microglia can be activated and recruited to action by neuronal injury or stimulation, such as axonal damage seen in MS or ischemic brain trauma resulting from stroke. These immunocompetent members of the CNS are also thought to have roles in synaptic plasticity under non-pathological conditions. We employ protocols for culturing microglia from the neonatal and adult tissues that are aimed to maximize the viable cell numbers while minimizing confounding variables, such as the presence of other CNS cell types and cell culture debris. We utilize large and easily discernable CNS components (e.g. cortex, spinal cord segments), which makes the entire process feasible and reproducible. The use of adult cells is a suitable alternative to the use of neonatal brain microglia, as many pathologies studied mainly affect the postnatal spinal cord. These culture systems are also useful for directly testing the effect of compounds that may either inhibit or promote microglial activation. Since microglial activation can shape the outcomes of disease in the adult CNS, there is a need for in vitro systems in which neonatal and adult microglia can be cultured and studied. 相似文献