全文获取类型
收费全文 | 82498篇 |
免费 | 5713篇 |
国内免费 | 3884篇 |
出版年
2023年 | 1175篇 |
2022年 | 1894篇 |
2021年 | 2655篇 |
2020年 | 2521篇 |
2019年 | 2958篇 |
2018年 | 2840篇 |
2017年 | 2041篇 |
2016年 | 2042篇 |
2015年 | 2622篇 |
2014年 | 4888篇 |
2013年 | 6134篇 |
2012年 | 3695篇 |
2011年 | 5042篇 |
2010年 | 3820篇 |
2009年 | 4223篇 |
2008年 | 4361篇 |
2007年 | 4421篇 |
2006年 | 3934篇 |
2005年 | 3534篇 |
2004年 | 3110篇 |
2003年 | 2687篇 |
2002年 | 2381篇 |
2001年 | 1623篇 |
2000年 | 1393篇 |
1999年 | 1401篇 |
1998年 | 1281篇 |
1997年 | 1109篇 |
1996年 | 1048篇 |
1995年 | 954篇 |
1994年 | 900篇 |
1993年 | 803篇 |
1992年 | 716篇 |
1991年 | 679篇 |
1990年 | 524篇 |
1989年 | 481篇 |
1988年 | 435篇 |
1987年 | 422篇 |
1986年 | 375篇 |
1985年 | 541篇 |
1984年 | 722篇 |
1983年 | 540篇 |
1982年 | 593篇 |
1981年 | 449篇 |
1980年 | 444篇 |
1979年 | 357篇 |
1978年 | 271篇 |
1977年 | 212篇 |
1976年 | 185篇 |
1975年 | 150篇 |
1974年 | 146篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
991.
Natural CO2 springs in Italy: a resource for examining long-term response of vegetation to rising atmospheric CO2 concentrations 总被引:2,自引:1,他引:1
It is estimated that more than 100 geothermal CO2 springs exist in central-western Italy. Eight springs were selected in which the atmospheric CO2 concentrations were consistently observed to be above the current atmospheric average of 354μmol mol-1. CO2 concentration measurements at some of the springs are reported. The springs are described, and their major topographic and vegetational features are reported. Preliminary observations made on natural vegetation growing around the gas vents are then illustrated. An azonal pattern of vegetation distribution occurs around every CO2 spring regardless of soil type and phytoclimatic areas. This is composed of pioneer populations of a Northern Eurasiatic species (Agrostis canina L.) which is often associated with Scirpus lacustris L. The potential of these sites for studying the long-term response of vegetation to rising atmospheric CO2 concentrations is discussed. 相似文献
992.
Christopher M. R. Bax Vijai S. Shankar A. S. M. Towhidul Alam Bridget E. Bax Baljit S. Moonga Christopher L. -H. Huang Mone Zaidi Barry R. Rifkin 《Bioscience reports》1993,13(3):169-174
We report the effects of tetracycline analogues on cytosolic Ca2+ transients resulting from application of ionic nickel (Ni2+), a potent surrogate agonist of the osteoclast Ca2+ receptor. Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca2+] response to an application of 5 mM-[Ni2+]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1–100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca2+]. The results suggest a novel action of tetracyclines on the osteoclast Ca2+ receptor. 相似文献
993.
Divalent cation and lipid-protein interactions of biomembranes 总被引:1,自引:0,他引:1
Divalent cations play an important role in the functions of biomembranes. This review deals with three topics: (1) Mg2+-mediated change in physical state of phospholipid induces conformation and activity change of reconstituted mitochondrial H+-ATPase, (2) a proper transmembrane Ca2+ gradient is essential for the higher enzymatic activity of adenylate cyclase, and (3) role of transmembrane Ca2+ gradient in the modulation of reconstituted sarcoplasmic reticulm Ca2+-ATPase activity. 相似文献
994.
995.
Inorganic cofactors (Mn, Ca2+ and Cl-) are essential for oxidation of H2O to O2 by Photosystem II. The Mn reductants NH2OH and its N-methyl derivatives have been employed as probes to further examine the interactions between these species and Mn at the active site of H2O oxidation. Results of these studies show that the size of a hydroxylamine derivative regulates its ability to inactivate O2 evolution activity, and that this size-dependent inhibition behavior arises from the protein structure of Photosystem II. A set of anions (Cl-, F- and SO4
2-) is able to slow NH2OH and CH3NHOH inactivation of intact Photosystem II membranes by exerting a stabilizing influence on the extrinsic 23 and 17 kDa polypeptides. In contrast to this non-specific anion effect, only Cl- is capable of attenuating CH3NHOH and (CH3)2NOH inhibition in salt-washed preparations lacking the 23 and 17 kDa polypeptides. However, Cl- fails to protect against NH2OH inhibition in salt-washed membranes. These results indicate that the attack by NH2OH and its N-methyl derivatives on Mn occurs at different sites in the O2-evolving complex. The small reductant NH2OH acts at a Cl--insensitive site whereas the inhibitions by CH3NHOH and (CH3)2NOH involve a site that is Cl- sensitive. These findings are consistent with earlier studies showing that the size of primary amines controls the Cl- sensitivity of their binding to Mn in the O2-evolving complex.Abbreviation MES
4-morpholinoethanesulfonic acid
- PS II
Photosystem II 相似文献
996.
Evaluation of the role of State transitions in determining the efficiency of light utilisation for CO2 assimilation in leaves 总被引:2,自引:0,他引:2
Wheat leaves were exposed to light treatments that excite preferentially Photosystem I (PS I) or Photosystem II (PS II) and induce State 1 or State 2, respectively. Simultaneous measurements of CO2 assimilation, chlorophyll fluorescence and absorbance at 820 nm were used to estimate the quantum efficiencies of CO2 assimilation and PS II and PS I photochemistry during State transitions. State transitions were found to be associated with changes in the efficiency with which an absorbed photon is transferred to an open PS II reaction centre, but did not correlate with changes in the quantum efficiencies of PS II photochemistry or CO2 assimilation. Studies of the phosphorylation status of the light harvesting chlorophyll protein complex associated with PS II (LHC II) in wheat leaves and using chlorina mutants of barley which are deficient in this complex demonstrate that the changes in the effective antennae size of Photosystem II occurring during State transitions require LHC II and correlate with the phosphorylation status of LHC II. However, such correlations were not found in maize leaves. It is concluded that State transitions in C3 leaves are associated with phosphorylation-induced modifications of the PS II antennae, but these changes do not serve to optimise the use of light absorbed by the leaf for CO2 assimilation.Abbreviations Fm, Fo, Fv
maximal, minimal and variable fluorescence yields
- Fm, Fv
maximal and variable fluorescence yields in a light adapted state
- LHC II
light harvesting chlorophyll a/b protein complex associated with PS II
- qP
photochemical quenching
- A820
light-induced absorbance change at 820 nm
- PS I, PS II
relative quantum efficiencies of PS I and PS II photochemistry
- CO
2
quantum yield of CO2 assimilation 相似文献
997.
Flash-induced, fast (t
1/2 1 ms), reversible reduction of the high potential cytochrome b-559 (cyt b-559HP) was observed in chloroplasts in the presence of 2 M protonophore, FCCP (carbonylcyanide p-trifluoromethoxyphenylhydrazone), CCCP (carbonylcyanide 3-chlorophenylhydrazone) or SF 6847 (2,6-di-(t-butyl)-4-(2,2-dicyanovinyl)phenol). These protonophores promote autooxidation of cyt b-559HP in the dark (Arnon and Tang 1988, Proc Natl Acad Sci USA 85: 9524). No fast photoreduction could, however, be observed if the molecules were oxidized with ferricyanide in the absence of protonophores. This suggests that the molecules must be deprotonated to be capable for fast photoreduction.Photoreduction of cyt b-559HP was largely insensitive to DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone), but was inhibited by DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea). With a train of flashes, no oscillation could be observed in the amplitudes of photoreduction. These data strongly suggest that cyt b-559HP is reduced by the semireduced secondary quinone acceptor (QB
–) of Photosystem 2.Abbreviations ADRY-
acceleration of the deactivation reactions of the water-splitting enzyme system Y of photosynthesis
- Ant 2p-
2-(3-chloro-4-trifluoromethyl)anilino-3,5-dinitrothiophene
- cyt-
cyto-chrome
- CCCP-
carbonylcyanide 3-chlorophenylhydrazone
- DBMIB-
2,5-dibromo-3-methyl-6-iso-propyl-p-benzoquinone
- DCMU-
3-(3,4-dichlorophenyl)-1,1-dimehtylurea
- FCCP-
carbonylcyanide p-trifluoromethoxyphenylhydrazone
- FeCy-
ferricyanide
- HP-
high potential form
- HQ-
hydroquinone
- PQ-
plastoquinone
- PS 2-
Photosystem 2
- SF 6847-
2,6-di-(t-butyl)-4-(2,2-dicyanovinyl)-phenol 相似文献
998.
cDNA cloning and expression of a potato (Solanum tuberosum) invertase 总被引:10,自引:0,他引:10
Peter E. Hedley Gordon C. Machray Howard V. Davies Lindsay Burch Robbie Waugh 《Plant molecular biology》1993,22(5):917-922
A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots. 相似文献
999.
Hatanaka Shin-Ichi Furukawa Jun Aoki Toshio Akatsuka Hirokazu Nagasawa Eiji 《Mycoscience》1994,35(4):391-394
Combining different chromatography systems, unusual nonprotein amino acids were isolated and unequivocally identified from a small amount (less than 100 g fresh weight) ofAmanita gymnopus fruit body. Without obtaining crystals of these amino acids, on the basis of1H-NMR determination, high resolution mass spectrometry, chlorine analysis and oxidation with L-amino acid oxidase, one of them proved to be a new chloroamino acid, (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid (G2). The other three were (2S)-2-amino-5-hexenoic acid (G1), (2S)-2-amino-4,5-hexadienoic acid (G3) and (2S)-2-amino-5-hexynoic acid (G4). Amino acid (G1) was also encountered for the first time in natural products. Amino acid (G3) has been reported from several kinds of fungi belonging toAmanita, subgenusLepidella. The occurrence of amino acid (G4) was already reported fromCortinarius claricolor.Part 23 in the series Biochemical studies of nitrogen compounds in fungi. Part 22, Hatanaka, S. I. et al. 1985. Trans. Mycol. Soc. Japan26: 61–68. 相似文献
1000.
DEREK TOBIN ANTHONY G. QUINN SHOSUKE ITO ANTHONY J. THODY 《Pigment cell & melanoma research》1994,7(4):204-209
The present study was carried out to investigate the abundance of tyrosinase and related proteins (TRP-1 and TRP-2) in human epidermis and their relationship to melanin type. Positive immunocytochemical staining was seen for all three proteins in epidermal melanocytes. For each protein the numbers of positively stained melanocytes were similar in all subjects studied irrespective of skin type. Following 5 daily suberythemal doses of UVB the melanocytes were larger, more dendritic, and increased in number. With TRP-1 and TRP-2 the increase in number in response to UVB was unrelated to skin type and, hence, with melanin type but with tyrosinase there was a much greater increase in skin types III and IV than in skin type I and II. The enhanced numbers of tyrosinase-positive melanocytes were accompanied by increased staining intensity, suggesting a greater expression of tyrosinase in the melanocytes from skin types III and IV compared with skin types I and II. This increase in tyrosinase could be related to the greater levels of eumelanin found in skin types III and IV, and this is in keeping with the view that higher levels of tyrosinase are associated with the production of eumelanin than phaeomelanin. 相似文献