全文获取类型
收费全文 | 1059篇 |
免费 | 101篇 |
国内免费 | 70篇 |
出版年
2024年 | 3篇 |
2023年 | 21篇 |
2022年 | 37篇 |
2021年 | 52篇 |
2020年 | 28篇 |
2019年 | 46篇 |
2018年 | 38篇 |
2017年 | 36篇 |
2016年 | 33篇 |
2015年 | 25篇 |
2014年 | 79篇 |
2013年 | 95篇 |
2012年 | 67篇 |
2011年 | 43篇 |
2010年 | 34篇 |
2009年 | 45篇 |
2008年 | 50篇 |
2007年 | 50篇 |
2006年 | 60篇 |
2005年 | 75篇 |
2004年 | 65篇 |
2003年 | 34篇 |
2002年 | 42篇 |
2001年 | 30篇 |
2000年 | 27篇 |
1999年 | 16篇 |
1998年 | 20篇 |
1997年 | 15篇 |
1996年 | 10篇 |
1995年 | 11篇 |
1994年 | 15篇 |
1993年 | 12篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有1230条查询结果,搜索用时 15 毫秒
81.
82.
83.
Gene-array technologies have been applied in a wide number of organisms to study gene expression profiling under several physiological and experimental conditions. Gene-array implementations combined with the information arising from emerging genome sequencing projects are expected to be in the near future a major tool to characterize genes involved in different processes. So far, gene expression profile studies in trypanosomatids have been performed in microarrays that use a glass support to immobilize fragments of genomic DNA followed by fluorescent detection. Here, we wanted to test the potential of genomic DNA macroarrays of Leishmania infantum using nylon membranes and radioactive detection. Nylon macroarrays present a number of advantages since the processing of the membranes is based on standard Southern blotting protocols familiar to molecular biologists, and the data acquisition equipment is available to most research institutions. Nylon macroarrays were employed to search for genes showing increased mRNA abundance during an axenic differentiation of L. infantum promastigotes to amastigotes. Several clones were rescued and, after validation by Northern blot assays, these L. infantum sequences were used to screen the Leishmania major gene database. The L. major contigs with high homology to the L. infantum sequences allowed a consistent identification of the regulated genes. 相似文献
84.
Pathway information is vital for successful quantitative modeling of biological systems. The almost 170 online pathway databases vary widely in coverage and representation of biological processes, making their use extremely difficult. Future pathway information systems for querying, visualization and analysis must support standard exchange formats to successfully integrate data on a large scale. Such integrated systems will greatly facilitate the constructive cycle of computational model building and experimental verification that lies at the heart of systems biology. 相似文献
85.
Zamyatnin AA 《Biochemistry. Biokhimii?a》2004,69(11):1276-1282
Some problems are considered which arise in biochemical studies on structure and function of natural oligopeptides consisting of 2–50 amino acid residues. The problems under consideration include the generation of oligopeptides from precursors, chemical structure, the role of functionally important radicals and spatial configuration, and structure-function relationships. Different types of regulation are shown mainly for oligopeptides involved in muscle contraction.Translated from Biokhimiya, Vol. 69, No. 11, 2004, pp. 1565–1573.Original Russian Text Copyright © 2004 by Zamyatnin. 相似文献
86.
87.
PHProteomicDB is a PHP-written module to help researchers in proteomics to share two-dimenslonal gel electrophoresis data using personal web sites. No technical or PHP knowledge is necessary except a few basics about web site management. PHProteomicDB has a user-friendly administration interface to enter and update data. It creates web pages on the fly displaying gel characteristics, gel pictures, and numbered gel spots with their related identifications pointing to their reference pages in protein databanks. The module is freely available at http://www.huvec.com/index.php3?rub=Download. 相似文献
88.
目的 通过讨论PACS高级预防维护技术,供同他人在日常维护过程中有一定地借鉴作用.方法 从PACS数据库性能检测与优化,存储设备检测及PACS各应用软件模块三方面进行阐述.结果 经过医院一线安装维护人员和PACS厂家多年实践的总结,拥有一定的可参考依据.结论 PACS的高级预防维护是一个复杂的系统工程,包括一般维护和高级维护在内的实践理论才刚刚起步,科学的、规范化的预防维护需要不断地完善和发展. 相似文献
89.
The ATP-binding cassette (ABC) transporters are one of the major classes of active transporters. They are widespread in archaea, bacteria, and eukaryota, indicating that they have arisen early in evolution. They are involved in many essential physiological processes, but the majority import or export a wide variety of compounds across cellular membranes. These systems share a common architecture composed of four (exporters) or five (importers) domains. To identify and reconstruct functional ABC transporters encoded by archaeal and bacterial genomes, we have developed a bioinformatic strategy. Cross-reference to the transport classification system is used to predict the type of compound transported. A high quality of annotation is achieved by manual verification of the predictions. However, in order to face the rapid increase in the number of published genomes, we also include analyses of genomes issuing directly from the automated strategy. Querying the database (http://www-abcdb.biotoul.fr) allows to easily retrieve ABC transporter repertories and related data. Additional query tools have been developed for the analysis of the ABC family from both functional and evolutionary perspectives. 相似文献
90.
Byung-Chul Choi Hang-Sik Shin Su-Yol Lee Tak Hur 《The International Journal of Life Cycle Assessment》2006,11(2):122-128
Background, Aims and Scope Telecommunication and information technology, dramatically emerged during the last decade, has generated environmental problems
by accelerating mass production, mass consumption, and mass disposal of personal computers (PCs) in Korea. In addition, it
has led the Korean new economy. The Korean government has encouraged researchers and industry to study the environmental impact,
adequate disposal treatment, and the reasonable recycling rate of an end-of-life personal computer. The main purpose of this
research is to investigate the life cycle environmental impact of PCs and to determine the desirable or feasible recycle rate
of an end-of-life PC. An LCA on a PC was performed based on different recycling scenario. Target audiences are new product
developers, designers, product recovery managers and environmental policy makers who are interested in the environmental impact
of PCs and recycling of end-of-life products.
Methods A target product is a Pentium IV personal computer made in Korea in 2001, excluding the monitor and peripheral equipment.
The procedure of the LCA followed the ISO14040 series. System boundary includes the entire life cycle of the product, including
pre-manufacturing (the electrical parts and components manufacturing), manufacturing, transportation, use, and disposal. The
LCI and impact assessment database for a PC was constructed using SIMAPRO version 4.0 software and LCI information was compiled
by site-specific data and the Korean national database. The LCA was performed on different recycling scenarios: one being
that of the current recycling rate of 46%, and the other being the ideal condition of a 100% recycling rate.
Results and Discussion Abiotic depletion, global warming, ecotoxicity, human toxicity, acidification, ozone layer depletion, photo-oxidant formation,
and eutrophication are adopted as the impact categories. The pre-manufacturing stage was a significant stage for all of the
environmental parameters, besides human toxicity potential. PC manufacturing consists of rather simple processes such as assembly
and packaging. For improving the environmental performance of PCs, environmental management approaches of design for the environment
and green procurement are recommended. The use stage had a significant potential due to the electricity consumption produced
by burning fossil fuel. The disposal stage's contribution to environmental impact was largest in human toxicity, and second
largest in ozone layer depletion potential. The PC recycling was shown to inhibit all environmental impacts with the exception
of the ozone depletion and ecotoxicity potential. The increase of light oil, nitric acid, sulfuric acid, and deoxidating agent
consumption during the recycling process contributes to the environmental impact of ozone and ecotoxicity parameters. Current
recovery and recycling technologies should be taken into account for enhancing the benefits of recycling. Anyway, the effectiveness
of recycling was highlighted by this study. PC recycling reduces the total environmental impact of the product. The PC recycling
is recommended to be raised up to at least 63% in order to reduce the environmental burdens of a PC in other life cycle stages.
Conclusion and Recommendation This study implies that design for the environment (DfE) in the product design stage and green procurement are recommended
for improving the entire environmental performance of electronic equipment such as PCs. The recycling of waste PCs clearly
reduces the environmental burden. There are, however, trade-offs among environmental parameters according to the PC recycling
rate. Current recycling methods are not effective in reducing ozone depletion and ecotoxicity environmental impact. The product
recovery is another key for efficient recycling. Efficient reverse logistics to collect and transport end-of-life PCs should
be taken into account to enhance recycling effects. There were several electrical parts not included in this assessment, due
to the unavailability of adequate data. Further studies with more detail and reliable inventories for electrical parts and
sub-components are recommended. Furthermore, costs of recycling should also be treated in further research. 相似文献